Pseudomonas phage Achelous

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Colwellvirinae; Uliginvirus; Pseudomonas virus Achelous

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GMU0|A0A2S1GMU0_9CAUD Uncharacterized protein OS=Pseudomonas phage Achelous OX=2163982 PE=4 SV=1
MM1 pKa = 7.53ARR3 pKa = 11.84MTVTFEE9 pKa = 3.59VDD11 pKa = 4.08QIEE14 pKa = 4.19EE15 pKa = 4.08DD16 pKa = 3.77CDD18 pKa = 3.82RR19 pKa = 11.84EE20 pKa = 4.18KK21 pKa = 11.17LALEE25 pKa = 5.4LIDD28 pKa = 5.17SLQAQYY34 pKa = 10.7PYY36 pKa = 10.54IDD38 pKa = 3.86INPEE42 pKa = 3.86HH43 pKa = 7.88VEE45 pKa = 4.15VEE47 pKa = 4.16

Molecular weight: 5.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GMT5|A0A2S1GMT5_9CAUD Uncharacterized protein OS=Pseudomonas phage Achelous OX=2163982 PE=4 SV=1
MM1 pKa = 7.87DD2 pKa = 4.17RR3 pKa = 11.84VKK5 pKa = 10.22TKK7 pKa = 10.9ASDD10 pKa = 3.55LPRR13 pKa = 11.84IKK15 pKa = 10.88AEE17 pKa = 5.24LIAKK21 pKa = 9.86QNGLCPITGRR31 pKa = 11.84SLRR34 pKa = 11.84GMTSSNVVVDD44 pKa = 4.81HH45 pKa = 5.89NHH47 pKa = 3.47QTGIIRR53 pKa = 11.84AALPRR58 pKa = 11.84GVNGLEE64 pKa = 3.85GKK66 pKa = 9.72LRR68 pKa = 11.84NLCIRR73 pKa = 11.84WGGCKK78 pKa = 7.33TQRR81 pKa = 11.84EE82 pKa = 4.58IIDD85 pKa = 3.78LLKK88 pKa = 11.17GLVAYY93 pKa = 10.3YY94 pKa = 9.27EE95 pKa = 4.15LHH97 pKa = 6.11GTPQTPYY104 pKa = 9.15VHH106 pKa = 6.6HH107 pKa = 7.02TFLTPTEE114 pKa = 4.08EE115 pKa = 4.07RR116 pKa = 11.84NKK118 pKa = 10.54RR119 pKa = 11.84NAAARR124 pKa = 11.84KK125 pKa = 8.67KK126 pKa = 10.07AAASRR131 pKa = 11.84ASS133 pKa = 3.2

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

14223

39

1260

302.6

33.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.323 ± 0.496

0.949 ± 0.133

6.581 ± 0.297

6.215 ± 0.299

3.944 ± 0.264

7.636 ± 0.243

2.341 ± 0.181

4.851 ± 0.223

5.59 ± 0.252

7.889 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.094 ± 0.203

4.486 ± 0.171

4.162 ± 0.257

4.612 ± 0.324

5.618 ± 0.22

5.154 ± 0.239

6.103 ± 0.302

6.609 ± 0.344

1.54 ± 0.152

3.305 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski