Amino acid dipepetide frequency for Pseudomonas phage Achelous

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.688AlaAla: 10.688 ± 1.181
0.281AlaCys: 0.281 ± 0.139
5.766AlaAsp: 5.766 ± 0.798
6.609AlaGlu: 6.609 ± 0.91
3.656AlaPhe: 3.656 ± 0.441
7.945AlaGly: 7.945 ± 1.137
1.758AlaHis: 1.758 ± 0.352
4.078AlaIle: 4.078 ± 0.515
4.781AlaLys: 4.781 ± 0.547
8.227AlaLeu: 8.227 ± 0.832
2.672AlaMet: 2.672 ± 0.387
4.43AlaAsn: 4.43 ± 0.431
3.164AlaPro: 3.164 ± 0.544
4.359AlaGln: 4.359 ± 0.645
5.414AlaArg: 5.414 ± 0.706
3.867AlaSer: 3.867 ± 0.612
5.695AlaThr: 5.695 ± 0.754
5.695AlaVal: 5.695 ± 0.557
1.125AlaTrp: 1.125 ± 0.376
3.023AlaTyr: 3.023 ± 0.413
0.0AlaXaa: 0.0 ± 0.0
Cys
0.422CysAla: 0.422 ± 0.239
0.141CysCys: 0.141 ± 0.12
0.984CysAsp: 0.984 ± 0.274
0.281CysGlu: 0.281 ± 0.167
0.422CysPhe: 0.422 ± 0.158
0.492CysGly: 0.492 ± 0.171
0.141CysHis: 0.141 ± 0.105
0.773CysIle: 0.773 ± 0.324
0.844CysLys: 0.844 ± 0.25
0.844CysLeu: 0.844 ± 0.244
0.211CysMet: 0.211 ± 0.105
0.492CysAsn: 0.492 ± 0.189
0.422CysPro: 0.422 ± 0.183
0.352CysGln: 0.352 ± 0.173
0.703CysArg: 0.703 ± 0.253
0.633CysSer: 0.633 ± 0.24
0.352CysThr: 0.352 ± 0.159
0.703CysVal: 0.703 ± 0.275
0.0CysTrp: 0.0 ± 0.0
0.281CysTyr: 0.281 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
5.977AspAla: 5.977 ± 0.58
0.492AspCys: 0.492 ± 0.178
4.711AspAsp: 4.711 ± 0.61
4.711AspGlu: 4.711 ± 0.507
2.672AspPhe: 2.672 ± 0.382
5.766AspGly: 5.766 ± 0.679
1.336AspHis: 1.336 ± 0.318
3.375AspIle: 3.375 ± 0.518
4.641AspLys: 4.641 ± 0.443
4.641AspLeu: 4.641 ± 0.62
1.547AspMet: 1.547 ± 0.302
1.969AspAsn: 1.969 ± 0.308
3.094AspPro: 3.094 ± 0.419
2.25AspGln: 2.25 ± 0.415
3.938AspArg: 3.938 ± 0.608
3.656AspSer: 3.656 ± 0.484
4.078AspThr: 4.078 ± 0.521
3.445AspVal: 3.445 ± 0.546
1.195AspTrp: 1.195 ± 0.299
2.32AspTyr: 2.32 ± 0.381
0.0AspXaa: 0.0 ± 0.0
Glu
7.102GluAla: 7.102 ± 0.896
0.633GluCys: 0.633 ± 0.247
4.359GluAsp: 4.359 ± 0.519
3.164GluGlu: 3.164 ± 0.535
2.531GluPhe: 2.531 ± 0.315
4.008GluGly: 4.008 ± 0.398
1.547GluHis: 1.547 ± 0.307
2.461GluIle: 2.461 ± 0.497
2.742GluLys: 2.742 ± 0.503
6.399GluLeu: 6.399 ± 0.625
2.25GluMet: 2.25 ± 0.265
2.039GluAsn: 2.039 ± 0.291
1.828GluPro: 1.828 ± 0.341
3.164GluGln: 3.164 ± 0.484
3.445GluArg: 3.445 ± 0.588
2.602GluSer: 2.602 ± 0.499
2.742GluThr: 2.742 ± 0.418
5.133GluVal: 5.133 ± 0.621
1.477GluTrp: 1.477 ± 0.379
2.531GluTyr: 2.531 ± 0.417
0.0GluXaa: 0.0 ± 0.0
Phe
2.813PheAla: 2.813 ± 0.462
0.352PheCys: 0.352 ± 0.18
3.445PheAsp: 3.445 ± 0.364
1.969PheGlu: 1.969 ± 0.359
2.18PhePhe: 2.18 ± 0.415
2.391PheGly: 2.391 ± 0.378
0.633PheHis: 0.633 ± 0.217
1.898PheIle: 1.898 ± 0.337
2.039PheLys: 2.039 ± 0.387
2.32PheLeu: 2.32 ± 0.37
1.336PheMet: 1.336 ± 0.282
2.672PheAsn: 2.672 ± 0.449
1.828PhePro: 1.828 ± 0.373
1.406PheGln: 1.406 ± 0.402
2.391PheArg: 2.391 ± 0.308
2.32PheSer: 2.32 ± 0.338
3.234PheThr: 3.234 ± 0.615
2.461PheVal: 2.461 ± 0.439
0.633PheTrp: 0.633 ± 0.167
1.125PheTyr: 1.125 ± 0.284
0.0PheXaa: 0.0 ± 0.0
Gly
5.625GlyAla: 5.625 ± 0.866
0.703GlyCys: 0.703 ± 0.221
5.133GlyAsp: 5.133 ± 0.575
4.781GlyGlu: 4.781 ± 0.621
3.375GlyPhe: 3.375 ± 0.527
6.961GlyGly: 6.961 ± 0.884
1.898GlyHis: 1.898 ± 0.449
3.797GlyIle: 3.797 ± 0.473
6.258GlyLys: 6.258 ± 0.77
5.203GlyLeu: 5.203 ± 0.695
2.18GlyMet: 2.18 ± 0.393
3.586GlyAsn: 3.586 ± 0.542
1.406GlyPro: 1.406 ± 0.263
3.375GlyGln: 3.375 ± 0.411
4.359GlyArg: 4.359 ± 0.467
4.078GlySer: 4.078 ± 0.486
4.149GlyThr: 4.149 ± 0.544
4.922GlyVal: 4.922 ± 0.551
1.758GlyTrp: 1.758 ± 0.311
2.813GlyTyr: 2.813 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
1.758HisAla: 1.758 ± 0.329
0.352HisCys: 0.352 ± 0.179
1.828HisAsp: 1.828 ± 0.362
0.984HisGlu: 0.984 ± 0.206
0.773HisPhe: 0.773 ± 0.217
2.039HisGly: 2.039 ± 0.386
0.492HisHis: 0.492 ± 0.198
0.703HisIle: 0.703 ± 0.193
1.898HisLys: 1.898 ± 0.389
2.883HisLeu: 2.883 ± 0.436
0.633HisMet: 0.633 ± 0.195
1.055HisAsn: 1.055 ± 0.331
1.195HisPro: 1.195 ± 0.344
1.125HisGln: 1.125 ± 0.262
0.844HisArg: 0.844 ± 0.22
1.195HisSer: 1.195 ± 0.221
1.125HisThr: 1.125 ± 0.242
1.055HisVal: 1.055 ± 0.294
0.492HisTrp: 0.492 ± 0.187
0.984HisTyr: 0.984 ± 0.277
0.0HisXaa: 0.0 ± 0.0
Ile
5.414IleAla: 5.414 ± 0.491
0.211IleCys: 0.211 ± 0.114
4.289IleAsp: 4.289 ± 0.53
3.023IleGlu: 3.023 ± 0.486
1.336IlePhe: 1.336 ± 0.296
3.797IleGly: 3.797 ± 0.503
1.406IleHis: 1.406 ± 0.381
2.742IleIle: 2.742 ± 0.779
2.953IleLys: 2.953 ± 0.38
2.602IleLeu: 2.602 ± 0.498
0.984IleMet: 0.984 ± 0.211
2.391IleAsn: 2.391 ± 0.397
2.461IlePro: 2.461 ± 0.395
2.32IleGln: 2.32 ± 0.476
3.023IleArg: 3.023 ± 0.399
2.32IleSer: 2.32 ± 0.487
3.234IleThr: 3.234 ± 0.462
2.391IleVal: 2.391 ± 0.412
0.633IleTrp: 0.633 ± 0.324
0.984IleTyr: 0.984 ± 0.223
0.0IleXaa: 0.0 ± 0.0
Lys
5.484LysAla: 5.484 ± 0.692
0.563LysCys: 0.563 ± 0.155
3.586LysAsp: 3.586 ± 0.518
4.008LysGlu: 4.008 ± 0.518
1.617LysPhe: 1.617 ± 0.33
4.008LysGly: 4.008 ± 0.66
1.055LysHis: 1.055 ± 0.256
2.32LysIle: 2.32 ± 0.343
2.109LysLys: 2.109 ± 0.345
5.274LysLeu: 5.274 ± 0.642
1.406LysMet: 1.406 ± 0.314
2.25LysAsn: 2.25 ± 0.427
2.672LysPro: 2.672 ± 0.391
2.742LysGln: 2.742 ± 0.45
3.797LysArg: 3.797 ± 0.593
2.32LysSer: 2.32 ± 0.266
3.023LysThr: 3.023 ± 0.394
4.149LysVal: 4.149 ± 0.464
0.773LysTrp: 0.773 ± 0.218
2.742LysTyr: 2.742 ± 0.405
0.0LysXaa: 0.0 ± 0.0
Leu
6.82LeuAla: 6.82 ± 0.694
1.125LeuCys: 1.125 ± 0.329
6.188LeuAsp: 6.188 ± 0.557
5.484LeuGlu: 5.484 ± 0.497
2.25LeuPhe: 2.25 ± 0.321
5.977LeuGly: 5.977 ± 0.603
2.18LeuHis: 2.18 ± 0.459
3.938LeuIle: 3.938 ± 0.482
3.445LeuLys: 3.445 ± 0.452
6.188LeuLeu: 6.188 ± 0.717
2.672LeuMet: 2.672 ± 0.472
3.375LeuAsn: 3.375 ± 0.436
3.445LeuPro: 3.445 ± 0.442
2.813LeuGln: 2.813 ± 0.544
5.203LeuArg: 5.203 ± 0.665
4.641LeuSer: 4.641 ± 0.494
5.695LeuThr: 5.695 ± 0.64
4.711LeuVal: 4.711 ± 0.539
0.773LeuTrp: 0.773 ± 0.201
1.898LeuTyr: 1.898 ± 0.284
0.0LeuXaa: 0.0 ± 0.0
Met
3.234MetAla: 3.234 ± 0.558
0.211MetCys: 0.211 ± 0.101
1.266MetAsp: 1.266 ± 0.255
2.602MetGlu: 2.602 ± 0.626
1.125MetPhe: 1.125 ± 0.198
1.969MetGly: 1.969 ± 0.302
0.563MetHis: 0.563 ± 0.217
0.984MetIle: 0.984 ± 0.281
1.898MetLys: 1.898 ± 0.41
1.898MetLeu: 1.898 ± 0.304
1.195MetMet: 1.195 ± 0.342
1.688MetAsn: 1.688 ± 0.347
1.055MetPro: 1.055 ± 0.349
1.547MetGln: 1.547 ± 0.238
1.969MetArg: 1.969 ± 0.33
2.531MetSer: 2.531 ± 0.403
2.25MetThr: 2.25 ± 0.288
1.828MetVal: 1.828 ± 0.34
0.281MetTrp: 0.281 ± 0.156
0.844MetTyr: 0.844 ± 0.219
0.0MetXaa: 0.0 ± 0.0
Asn
3.234AsnAla: 3.234 ± 0.628
0.211AsnCys: 0.211 ± 0.133
2.25AsnAsp: 2.25 ± 0.398
1.969AsnGlu: 1.969 ± 0.348
2.461AsnPhe: 2.461 ± 0.402
3.656AsnGly: 3.656 ± 0.408
0.773AsnHis: 0.773 ± 0.204
2.039AsnIle: 2.039 ± 0.408
2.813AsnLys: 2.813 ± 0.393
3.797AsnLeu: 3.797 ± 0.469
1.617AsnMet: 1.617 ± 0.303
1.758AsnAsn: 1.758 ± 0.344
3.023AsnPro: 3.023 ± 0.468
2.039AsnGln: 2.039 ± 0.385
2.672AsnArg: 2.672 ± 0.519
2.742AsnSer: 2.742 ± 0.42
2.883AsnThr: 2.883 ± 0.305
2.813AsnVal: 2.813 ± 0.406
0.703AsnTrp: 0.703 ± 0.244
1.406AsnTyr: 1.406 ± 0.305
0.0AsnXaa: 0.0 ± 0.0
Pro
3.867ProAla: 3.867 ± 0.459
0.492ProCys: 0.492 ± 0.222
2.039ProAsp: 2.039 ± 0.425
3.305ProGlu: 3.305 ± 0.512
1.828ProPhe: 1.828 ± 0.386
1.266ProGly: 1.266 ± 0.32
1.266ProHis: 1.266 ± 0.331
2.18ProIle: 2.18 ± 0.448
2.391ProLys: 2.391 ± 0.324
2.883ProLeu: 2.883 ± 0.534
1.055ProMet: 1.055 ± 0.202
2.672ProAsn: 2.672 ± 0.496
1.969ProPro: 1.969 ± 0.318
1.828ProGln: 1.828 ± 0.234
1.547ProArg: 1.547 ± 0.264
2.25ProSer: 2.25 ± 0.364
2.672ProThr: 2.672 ± 0.44
3.516ProVal: 3.516 ± 0.422
0.703ProTrp: 0.703 ± 0.193
1.898ProTyr: 1.898 ± 0.407
0.0ProXaa: 0.0 ± 0.0
Gln
5.977GlnAla: 5.977 ± 0.733
0.492GlnCys: 0.492 ± 0.158
2.531GlnAsp: 2.531 ± 0.595
2.391GlnGlu: 2.391 ± 0.36
2.039GlnPhe: 2.039 ± 0.332
3.938GlnGly: 3.938 ± 0.438
0.914GlnHis: 0.914 ± 0.206
2.602GlnIle: 2.602 ± 0.386
1.828GlnLys: 1.828 ± 0.339
3.797GlnLeu: 3.797 ± 0.77
1.406GlnMet: 1.406 ± 0.428
0.984GlnAsn: 0.984 ± 0.213
1.195GlnPro: 1.195 ± 0.19
3.445GlnGln: 3.445 ± 0.623
2.461GlnArg: 2.461 ± 0.371
2.32GlnSer: 2.32 ± 0.493
2.672GlnThr: 2.672 ± 0.562
3.234GlnVal: 3.234 ± 0.518
0.563GlnTrp: 0.563 ± 0.182
1.336GlnTyr: 1.336 ± 0.286
0.0GlnXaa: 0.0 ± 0.0
Arg
5.063ArgAla: 5.063 ± 0.483
0.773ArgCys: 0.773 ± 0.238
3.516ArgAsp: 3.516 ± 0.53
3.164ArgGlu: 3.164 ± 0.503
1.969ArgPhe: 1.969 ± 0.359
4.219ArgGly: 4.219 ± 0.595
1.406ArgHis: 1.406 ± 0.281
2.531ArgIle: 2.531 ± 0.302
3.094ArgLys: 3.094 ± 0.328
4.641ArgLeu: 4.641 ± 0.517
2.18ArgMet: 2.18 ± 0.378
3.164ArgAsn: 3.164 ± 0.504
1.969ArgPro: 1.969 ± 0.439
2.953ArgGln: 2.953 ± 0.415
2.883ArgArg: 2.883 ± 0.492
2.039ArgSer: 2.039 ± 0.312
3.164ArgThr: 3.164 ± 0.64
5.063ArgVal: 5.063 ± 0.696
0.914ArgTrp: 0.914 ± 0.233
1.477ArgTyr: 1.477 ± 0.295
0.0ArgXaa: 0.0 ± 0.0
Ser
4.57SerAla: 4.57 ± 0.771
0.352SerCys: 0.352 ± 0.139
3.516SerAsp: 3.516 ± 0.368
2.953SerGlu: 2.953 ± 0.421
2.602SerPhe: 2.602 ± 0.451
3.656SerGly: 3.656 ± 0.528
1.125SerHis: 1.125 ± 0.334
2.883SerIle: 2.883 ± 0.398
2.672SerLys: 2.672 ± 0.372
4.43SerLeu: 4.43 ± 0.496
1.898SerMet: 1.898 ± 0.468
1.406SerAsn: 1.406 ± 0.363
2.25SerPro: 2.25 ± 0.299
2.391SerGln: 2.391 ± 0.441
3.375SerArg: 3.375 ± 0.43
2.32SerSer: 2.32 ± 0.483
3.023SerThr: 3.023 ± 0.474
3.797SerVal: 3.797 ± 0.472
0.633SerTrp: 0.633 ± 0.207
1.688SerTyr: 1.688 ± 0.341
0.0SerXaa: 0.0 ± 0.0
Thr
5.274ThrAla: 5.274 ± 0.556
0.914ThrCys: 0.914 ± 0.3
3.586ThrAsp: 3.586 ± 0.487
3.938ThrGlu: 3.938 ± 0.459
2.531ThrPhe: 2.531 ± 0.456
6.399ThrGly: 6.399 ± 0.558
1.898ThrHis: 1.898 ± 0.324
4.078ThrIle: 4.078 ± 0.536
3.164ThrLys: 3.164 ± 0.505
4.641ThrLeu: 4.641 ± 0.568
2.109ThrMet: 2.109 ± 0.427
2.742ThrAsn: 2.742 ± 0.405
3.164ThrPro: 3.164 ± 0.479
2.391ThrGln: 2.391 ± 0.457
2.391ThrArg: 2.391 ± 0.341
2.672ThrSer: 2.672 ± 0.438
3.586ThrThr: 3.586 ± 0.551
3.234ThrVal: 3.234 ± 0.574
0.352ThrTrp: 0.352 ± 0.129
1.969ThrTyr: 1.969 ± 0.355
0.0ThrXaa: 0.0 ± 0.0
Val
5.484ValAla: 5.484 ± 0.748
0.633ValCys: 0.633 ± 0.192
3.938ValAsp: 3.938 ± 0.529
4.149ValGlu: 4.149 ± 0.609
2.531ValPhe: 2.531 ± 0.407
4.149ValGly: 4.149 ± 0.643
1.547ValHis: 1.547 ± 0.424
3.164ValIle: 3.164 ± 0.504
3.938ValLys: 3.938 ± 0.65
4.43ValLeu: 4.43 ± 0.708
2.039ValMet: 2.039 ± 0.322
3.305ValAsn: 3.305 ± 0.558
3.305ValPro: 3.305 ± 0.426
3.445ValGln: 3.445 ± 0.573
2.953ValArg: 2.953 ± 0.574
4.219ValSer: 4.219 ± 0.595
3.938ValThr: 3.938 ± 0.602
5.274ValVal: 5.274 ± 0.611
1.266ValTrp: 1.266 ± 0.252
2.391ValTyr: 2.391 ± 0.519
0.0ValXaa: 0.0 ± 0.0
Trp
1.617TrpAla: 1.617 ± 0.317
0.281TrpCys: 0.281 ± 0.147
0.633TrpAsp: 0.633 ± 0.183
1.125TrpGlu: 1.125 ± 0.196
0.633TrpPhe: 0.633 ± 0.217
0.914TrpGly: 0.914 ± 0.198
0.563TrpHis: 0.563 ± 0.165
0.492TrpIle: 0.492 ± 0.173
0.703TrpLys: 0.703 ± 0.175
1.055TrpLeu: 1.055 ± 0.236
0.422TrpMet: 0.422 ± 0.149
0.703TrpAsn: 0.703 ± 0.256
0.914TrpPro: 0.914 ± 0.292
0.703TrpGln: 0.703 ± 0.25
0.984TrpArg: 0.984 ± 0.244
0.914TrpSer: 0.914 ± 0.263
0.844TrpThr: 0.844 ± 0.235
0.984TrpVal: 0.984 ± 0.282
0.352TrpTrp: 0.352 ± 0.161
0.563TrpTyr: 0.563 ± 0.285
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.813TyrAla: 2.813 ± 0.565
0.281TyrCys: 0.281 ± 0.132
2.25TyrAsp: 2.25 ± 0.522
1.547TyrGlu: 1.547 ± 0.356
0.914TyrPhe: 0.914 ± 0.25
2.813TyrGly: 2.813 ± 0.36
0.914TyrHis: 0.914 ± 0.338
1.477TyrIle: 1.477 ± 0.342
1.688TyrLys: 1.688 ± 0.417
2.742TyrLeu: 2.742 ± 0.376
1.055TyrMet: 1.055 ± 0.259
2.18TyrAsn: 2.18 ± 0.407
1.266TyrPro: 1.266 ± 0.413
1.477TyrGln: 1.477 ± 0.378
1.828TyrArg: 1.828 ± 0.38
1.898TyrSer: 1.898 ± 0.388
2.672TyrThr: 2.672 ± 0.439
1.688TyrVal: 1.688 ± 0.357
0.773TyrTrp: 0.773 ± 0.209
0.773TyrTyr: 0.773 ± 0.252
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 47 proteins (14223 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski