Schumannella sp. 10F1D-1
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3272 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A506Y1L2|A0A506Y1L2_9MICO Class I SAM-dependent methyltransferase OS=Schumannella sp. 10F1D-1 OX=2590779 GN=FJ657_06360 PE=4 SV=1
MM1 pKa = 7.64 RR2 pKa = 11.84 PARR5 pKa = 11.84 ILIAALAAATLVTTLSGCALIDD27 pKa = 3.41 TARR30 pKa = 11.84 DD31 pKa = 3.12 LGRR34 pKa = 11.84 ALGGGGAPGSGGSVDD49 pKa = 4.01 PDD51 pKa = 3.45 YY52 pKa = 11.03 DD53 pKa = 3.69 TALDD57 pKa = 3.76 EE58 pKa = 5.78 LEE60 pKa = 4.81 VGDD63 pKa = 5.69 CFLEE67 pKa = 4.58 PDD69 pKa = 4.63 DD70 pKa = 6.11 DD71 pKa = 4.47 IEE73 pKa = 4.24 TAIVDD78 pKa = 3.84 CSDD81 pKa = 3.1 EE82 pKa = 3.97 HH83 pKa = 8.63 DD84 pKa = 4.32 YY85 pKa = 11.53 EE86 pKa = 4.41 VTALVTLEE94 pKa = 4.05 GVDD97 pKa = 3.66 YY98 pKa = 10.97 PGDD101 pKa = 3.51 IKK103 pKa = 11.12 VDD105 pKa = 3.32 KK106 pKa = 10.54 LSRR109 pKa = 11.84 ALCSQALTDD118 pKa = 4.02 YY119 pKa = 11.37 VGIPYY124 pKa = 9.88 SDD126 pKa = 4.02 SSLEE130 pKa = 4.01 SWAWTPDD137 pKa = 3.01 EE138 pKa = 5.85 DD139 pKa = 3.6 GWADD143 pKa = 3.44 GDD145 pKa = 4.16 HH146 pKa = 6.39 VAVCTATDD154 pKa = 3.61 YY155 pKa = 11.59 YY156 pKa = 11.39 YY157 pKa = 11.01 DD158 pKa = 4.06 VLDD161 pKa = 4.25 QSVKK165 pKa = 10.44 DD166 pKa = 3.66 ARR168 pKa = 11.84 II169 pKa = 3.32
Molecular weight: 17.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.63
IPC_protein 3.656
Toseland 3.414
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.478
Grimsley 3.325
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 4.062
Thurlkill 3.49
EMBOSS 3.643
Sillero 3.783
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.745
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|A0A506XVG2|A0A506XVG2_9MICO L-rhamnose mutarotase OS=Schumannella sp. 10F1D-1 OX=2590779 GN=FJ657_16105 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3272
0
3272
1081973
25
6143
330.7
35.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.19 ± 0.061
0.406 ± 0.009
6.364 ± 0.043
5.334 ± 0.049
2.923 ± 0.028
9.377 ± 0.051
1.843 ± 0.02
4.432 ± 0.036
2.041 ± 0.032
10.063 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.483 ± 0.019
1.843 ± 0.03
5.501 ± 0.034
2.768 ± 0.025
7.385 ± 0.058
5.858 ± 0.043
6.136 ± 0.089
8.812 ± 0.036
1.408 ± 0.015
1.832 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here