Arthrobacter sp. RIT-PI-e

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; unclassified Arthrobacter

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0BBL2|A0A0L0BBL2_9MICC SCP_3 domain-containing protein OS=Arthrobacter sp. RIT-PI-e OX=1681197 GN=AC792_14595 PE=4 SV=1
MM1 pKa = 7.33SRR3 pKa = 11.84GGPGYY8 pKa = 10.17GDD10 pKa = 3.18PVGDD14 pKa = 3.33VRR16 pKa = 11.84RR17 pKa = 11.84AGRR20 pKa = 11.84SLLLVTVLALASCASQDD37 pKa = 3.2GSGNAFPGGAVPDD50 pKa = 4.03YY51 pKa = 10.52QLGGAYY57 pKa = 8.14EE58 pKa = 4.2PPADD62 pKa = 3.44VGVVVRR68 pKa = 11.84DD69 pKa = 3.91ASDD72 pKa = 3.42EE73 pKa = 4.04PAEE76 pKa = 4.66GIYY79 pKa = 10.07SICYY83 pKa = 9.87LNAFQTQPGEE93 pKa = 4.1EE94 pKa = 4.24RR95 pKa = 11.84SWLAADD101 pKa = 4.95LVLEE105 pKa = 4.19VDD107 pKa = 4.68GEE109 pKa = 4.5PVTDD113 pKa = 4.51PDD115 pKa = 3.78WPDD118 pKa = 3.2EE119 pKa = 4.02YY120 pKa = 11.64LLDD123 pKa = 3.92TGTDD127 pKa = 3.16AKK129 pKa = 10.4RR130 pKa = 11.84AAVADD135 pKa = 5.13RR136 pKa = 11.84IGTAIDD142 pKa = 3.46GCADD146 pKa = 3.43SGFDD150 pKa = 5.22AVEE153 pKa = 4.06LDD155 pKa = 3.9NLDD158 pKa = 3.26SYY160 pKa = 11.47LRR162 pKa = 11.84SEE164 pKa = 4.81GRR166 pKa = 11.84LTLDD170 pKa = 4.12DD171 pKa = 4.24NVMLARR177 pKa = 11.84TLAEE181 pKa = 4.02RR182 pKa = 11.84AHH184 pKa = 7.04DD185 pKa = 4.42LGLQVGQKK193 pKa = 9.7NAAEE197 pKa = 4.04EE198 pKa = 4.18SAALQDD204 pKa = 3.49AGFDD208 pKa = 3.54FAVAEE213 pKa = 4.03QCVEE217 pKa = 4.1FEE219 pKa = 4.25EE220 pKa = 5.48CDD222 pKa = 3.32AYY224 pKa = 10.25TDD226 pKa = 3.82VYY228 pKa = 11.09GEE230 pKa = 4.07AVLAVEE236 pKa = 4.49YY237 pKa = 9.97PEE239 pKa = 5.14EE240 pKa = 5.09SGLPDD245 pKa = 3.59PCAVDD250 pKa = 3.8GRR252 pKa = 11.84PASTVLRR259 pKa = 11.84DD260 pKa = 3.8LEE262 pKa = 4.71LVTPADD268 pKa = 3.29PGYY271 pKa = 10.87LYY273 pKa = 10.4RR274 pKa = 11.84ACC276 pKa = 5.04

Molecular weight: 29.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0BM10|A0A0L0BM10_9MICC XRE family transcriptional regulator OS=Arthrobacter sp. RIT-PI-e OX=1681197 GN=AC792_02000 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.31VRR4 pKa = 11.84NSLRR8 pKa = 11.84ALKK11 pKa = 9.69KK12 pKa = 9.55IPGSQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 8.58KK31 pKa = 9.2NPRR34 pKa = 11.84IKK36 pKa = 10.52ARR38 pKa = 11.84QGG40 pKa = 2.94

Molecular weight: 4.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2792

0

2792

870992

37

1536

312.0

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.668 ± 0.074

0.562 ± 0.01

5.992 ± 0.039

5.821 ± 0.049

3.086 ± 0.029

9.401 ± 0.041

2.092 ± 0.022

4.118 ± 0.034

1.915 ± 0.036

10.492 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.849 ± 0.018

2.032 ± 0.027

5.576 ± 0.039

2.912 ± 0.024

7.19 ± 0.046

5.774 ± 0.033

6.251 ± 0.038

8.906 ± 0.037

1.351 ± 0.02

2.01 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski