Amino acid dipepetide frequency for Arthrobacter sp. RIT-PI-e

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.687AlaAla: 18.687 ± 0.207
0.701AlaCys: 0.701 ± 0.026
7.615AlaAsp: 7.615 ± 0.106
7.839AlaGlu: 7.839 ± 0.105
3.643AlaPhe: 3.643 ± 0.078
12.811AlaGly: 12.811 ± 0.144
2.335AlaHis: 2.335 ± 0.055
4.297AlaIle: 4.297 ± 0.092
2.295AlaLys: 2.295 ± 0.064
13.541AlaLeu: 13.541 ± 0.151
2.567AlaMet: 2.567 ± 0.058
2.11AlaAsn: 2.11 ± 0.049
6.55AlaPro: 6.55 ± 0.126
3.669AlaGln: 3.669 ± 0.068
8.702AlaArg: 8.702 ± 0.109
6.79AlaSer: 6.79 ± 0.084
6.555AlaThr: 6.555 ± 0.101
12.047AlaVal: 12.047 ± 0.128
1.636AlaTrp: 1.636 ± 0.053
2.287AlaTyr: 2.287 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.707CysAla: 0.707 ± 0.03
0.044CysCys: 0.044 ± 0.007
0.313CysAsp: 0.313 ± 0.019
0.278CysGlu: 0.278 ± 0.018
0.179CysPhe: 0.179 ± 0.015
0.661CysGly: 0.661 ± 0.025
0.124CysHis: 0.124 ± 0.012
0.212CysIle: 0.212 ± 0.015
0.061CysLys: 0.061 ± 0.009
0.53CysLeu: 0.53 ± 0.029
0.107CysMet: 0.107 ± 0.012
0.104CysAsn: 0.104 ± 0.011
0.35CysPro: 0.35 ± 0.023
0.116CysGln: 0.116 ± 0.011
0.387CysArg: 0.387 ± 0.021
0.38CysSer: 0.38 ± 0.024
0.406CysThr: 0.406 ± 0.025
0.443CysVal: 0.443 ± 0.023
0.084CysTrp: 0.084 ± 0.012
0.134CysTyr: 0.134 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
8.242AspAla: 8.242 ± 0.118
0.262AspCys: 0.262 ± 0.018
3.873AspAsp: 3.873 ± 0.091
3.636AspGlu: 3.636 ± 0.074
1.741AspPhe: 1.741 ± 0.053
6.149AspGly: 6.149 ± 0.109
1.35AspHis: 1.35 ± 0.04
2.172AspIle: 2.172 ± 0.056
0.928AspLys: 0.928 ± 0.043
6.432AspLeu: 6.432 ± 0.099
0.836AspMet: 0.836 ± 0.029
0.96AspAsn: 0.96 ± 0.031
4.195AspPro: 4.195 ± 0.074
1.609AspGln: 1.609 ± 0.046
4.03AspArg: 4.03 ± 0.067
2.635AspSer: 2.635 ± 0.064
3.382AspThr: 3.382 ± 0.063
5.45AspVal: 5.45 ± 0.084
0.798AspTrp: 0.798 ± 0.03
1.241AspTyr: 1.241 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.912GluAla: 6.912 ± 0.096
0.276GluCys: 0.276 ± 0.019
3.891GluAsp: 3.891 ± 0.069
3.89GluGlu: 3.89 ± 0.088
1.637GluPhe: 1.637 ± 0.05
4.634GluGly: 4.634 ± 0.081
1.574GluHis: 1.574 ± 0.046
2.504GluIle: 2.504 ± 0.059
1.363GluLys: 1.363 ± 0.045
6.758GluLeu: 6.758 ± 0.099
0.875GluMet: 0.875 ± 0.034
1.406GluAsn: 1.406 ± 0.046
2.937GluPro: 2.937 ± 0.066
2.6GluGln: 2.6 ± 0.057
4.674GluArg: 4.674 ± 0.087
2.724GluSer: 2.724 ± 0.064
2.822GluThr: 2.822 ± 0.064
4.876GluVal: 4.876 ± 0.087
0.661GluTrp: 0.661 ± 0.031
1.192GluTyr: 1.192 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.742PheAla: 3.742 ± 0.078
0.21PheCys: 0.21 ± 0.013
2.247PheAsp: 2.247 ± 0.054
1.773PheGlu: 1.773 ± 0.045
1.124PhePhe: 1.124 ± 0.041
3.348PheGly: 3.348 ± 0.066
0.561PheHis: 0.561 ± 0.029
1.111PheIle: 1.111 ± 0.042
0.529PheLys: 0.529 ± 0.025
3.101PheLeu: 3.101 ± 0.065
0.514PheMet: 0.514 ± 0.022
0.723PheAsn: 0.723 ± 0.028
1.406PhePro: 1.406 ± 0.043
0.722PheGln: 0.722 ± 0.031
1.858PheArg: 1.858 ± 0.044
1.944PheSer: 1.944 ± 0.052
2.202PheThr: 2.202 ± 0.048
2.651PheVal: 2.651 ± 0.056
0.443PheTrp: 0.443 ± 0.022
0.65PheTyr: 0.65 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
11.238GlyAla: 11.238 ± 0.115
0.64GlyCys: 0.64 ± 0.026
4.522GlyAsp: 4.522 ± 0.088
5.09GlyGlu: 5.09 ± 0.096
3.242GlyPhe: 3.242 ± 0.062
8.377GlyGly: 8.377 ± 0.11
1.951GlyHis: 1.951 ± 0.048
4.579GlyIle: 4.579 ± 0.086
2.141GlyLys: 2.141 ± 0.056
9.395GlyLeu: 9.395 ± 0.122
2.029GlyMet: 2.029 ± 0.051
2.022GlyAsn: 2.022 ± 0.056
4.465GlyPro: 4.465 ± 0.084
2.628GlyGln: 2.628 ± 0.058
6.985GlyArg: 6.985 ± 0.099
6.255GlySer: 6.255 ± 0.097
6.659GlyThr: 6.659 ± 0.092
8.111GlyVal: 8.111 ± 0.098
1.472GlyTrp: 1.472 ± 0.047
2.215GlyTyr: 2.215 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.392HisAla: 2.392 ± 0.055
0.14HisCys: 0.14 ± 0.015
1.185HisAsp: 1.185 ± 0.035
1.199HisGlu: 1.199 ± 0.044
0.605HisPhe: 0.605 ± 0.027
2.16HisGly: 2.16 ± 0.058
0.635HisHis: 0.635 ± 0.029
0.687HisIle: 0.687 ± 0.027
0.284HisLys: 0.284 ± 0.018
2.321HisLeu: 2.321 ± 0.05
0.328HisMet: 0.328 ± 0.02
0.355HisAsn: 0.355 ± 0.023
1.631HisPro: 1.631 ± 0.048
0.586HisGln: 0.586 ± 0.029
1.807HisArg: 1.807 ± 0.046
1.02HisSer: 1.02 ± 0.04
1.139HisThr: 1.139 ± 0.035
1.708HisVal: 1.708 ± 0.052
0.295HisTrp: 0.295 ± 0.02
0.441HisTyr: 0.441 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.418IleAla: 5.418 ± 0.09
0.293IleCys: 0.293 ± 0.019
2.945IleAsp: 2.945 ± 0.064
2.435IleGlu: 2.435 ± 0.053
1.209IlePhe: 1.209 ± 0.046
4.354IleGly: 4.354 ± 0.089
0.712IleHis: 0.712 ± 0.031
1.75IleIle: 1.75 ± 0.052
0.78IleLys: 0.78 ± 0.034
3.826IleLeu: 3.826 ± 0.082
0.656IleMet: 0.656 ± 0.03
0.937IleAsn: 0.937 ± 0.033
2.237IlePro: 2.237 ± 0.052
0.894IleGln: 0.894 ± 0.034
2.638IleArg: 2.638 ± 0.063
2.413IleSer: 2.413 ± 0.058
3.052IleThr: 3.052 ± 0.064
3.506IleVal: 3.506 ± 0.075
0.44IleTrp: 0.44 ± 0.026
0.687IleTyr: 0.687 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
2.318LysAla: 2.318 ± 0.071
0.064LysCys: 0.064 ± 0.009
1.28LysAsp: 1.28 ± 0.037
1.115LysGlu: 1.115 ± 0.045
0.51LysPhe: 0.51 ± 0.024
1.553LysGly: 1.553 ± 0.041
0.413LysHis: 0.413 ± 0.021
0.975LysIle: 0.975 ± 0.036
0.669LysLys: 0.669 ± 0.031
1.916LysLeu: 1.916 ± 0.054
0.379LysMet: 0.379 ± 0.025
0.504LysAsn: 0.504 ± 0.026
1.113LysPro: 1.113 ± 0.036
0.656LysGln: 0.656 ± 0.029
1.367LysArg: 1.367 ± 0.044
1.025LysSer: 1.025 ± 0.037
1.024LysThr: 1.024 ± 0.041
1.603LysVal: 1.603 ± 0.051
0.18LysTrp: 0.18 ± 0.014
0.488LysTyr: 0.488 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.014LeuAla: 14.014 ± 0.153
0.568LeuCys: 0.568 ± 0.026
6.513LeuAsp: 6.513 ± 0.107
5.827LeuGlu: 5.827 ± 0.085
2.938LeuPhe: 2.938 ± 0.068
9.932LeuGly: 9.932 ± 0.132
2.127LeuHis: 2.127 ± 0.058
4.17LeuIle: 4.17 ± 0.093
1.804LeuLys: 1.804 ± 0.043
11.241LeuLeu: 11.241 ± 0.188
1.777LeuMet: 1.777 ± 0.051
1.894LeuAsn: 1.894 ± 0.052
6.109LeuPro: 6.109 ± 0.097
2.883LeuGln: 2.883 ± 0.062
7.636LeuArg: 7.636 ± 0.106
5.781LeuSer: 5.781 ± 0.089
7.029LeuThr: 7.029 ± 0.089
9.773LeuVal: 9.773 ± 0.123
1.165LeuTrp: 1.165 ± 0.044
1.737LeuTyr: 1.737 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.177MetAla: 2.177 ± 0.047
0.094MetCys: 0.094 ± 0.009
0.968MetAsp: 0.968 ± 0.034
0.83MetGlu: 0.83 ± 0.031
0.551MetPhe: 0.551 ± 0.028
1.535MetGly: 1.535 ± 0.041
0.356MetHis: 0.356 ± 0.019
0.947MetIle: 0.947 ± 0.039
0.456MetLys: 0.456 ± 0.028
1.831MetLeu: 1.831 ± 0.057
0.349MetMet: 0.349 ± 0.018
0.509MetAsn: 0.509 ± 0.023
1.026MetPro: 1.026 ± 0.031
0.54MetGln: 0.54 ± 0.026
1.23MetArg: 1.23 ± 0.039
1.472MetSer: 1.472 ± 0.042
1.574MetThr: 1.574 ± 0.043
1.552MetVal: 1.552 ± 0.045
0.193MetTrp: 0.193 ± 0.016
0.303MetTyr: 0.303 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.358AsnAla: 2.358 ± 0.057
0.127AsnCys: 0.127 ± 0.011
1.09AsnAsp: 1.09 ± 0.036
1.04AsnGlu: 1.04 ± 0.035
0.652AsnPhe: 0.652 ± 0.032
1.915AsnGly: 1.915 ± 0.046
0.433AsnHis: 0.433 ± 0.023
0.902AsnIle: 0.902 ± 0.035
0.382AsnLys: 0.382 ± 0.025
2.135AsnLeu: 2.135 ± 0.048
0.351AsnMet: 0.351 ± 0.023
0.53AsnAsn: 0.53 ± 0.03
1.596AsnPro: 1.596 ± 0.043
0.609AsnGln: 0.609 ± 0.028
1.379AsnArg: 1.379 ± 0.037
1.061AsnSer: 1.061 ± 0.036
1.285AsnThr: 1.285 ± 0.047
1.666AsnVal: 1.666 ± 0.049
0.312AsnTrp: 0.312 ± 0.02
0.499AsnTyr: 0.499 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
7.765ProAla: 7.765 ± 0.121
0.218ProCys: 0.218 ± 0.015
3.831ProAsp: 3.831 ± 0.078
4.167ProGlu: 4.167 ± 0.06
1.747ProPhe: 1.747 ± 0.042
5.956ProGly: 5.956 ± 0.108
1.149ProHis: 1.149 ± 0.034
1.652ProIle: 1.652 ± 0.045
0.907ProLys: 0.907 ± 0.035
5.169ProLeu: 5.169 ± 0.082
1.005ProMet: 1.005 ± 0.034
0.893ProAsn: 0.893 ± 0.032
2.7ProPro: 2.7 ± 0.092
1.498ProGln: 1.498 ± 0.042
3.61ProArg: 3.61 ± 0.068
3.31ProSer: 3.31 ± 0.072
3.107ProThr: 3.107 ± 0.073
5.242ProVal: 5.242 ± 0.07
0.773ProTrp: 0.773 ± 0.03
1.062ProTyr: 1.062 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.491GlnAla: 3.491 ± 0.066
0.114GlnCys: 0.114 ± 0.011
1.791GlnAsp: 1.791 ± 0.051
1.78GlnGlu: 1.78 ± 0.045
0.823GlnPhe: 0.823 ± 0.03
2.441GlnGly: 2.441 ± 0.064
0.747GlnHis: 0.747 ± 0.032
1.274GlnIle: 1.274 ± 0.04
0.617GlnLys: 0.617 ± 0.027
3.366GlnLeu: 3.366 ± 0.063
0.516GlnMet: 0.516 ± 0.028
0.611GlnAsn: 0.611 ± 0.028
1.488GlnPro: 1.488 ± 0.045
1.352GlnGln: 1.352 ± 0.05
2.401GlnArg: 2.401 ± 0.053
1.372GlnSer: 1.372 ± 0.047
1.303GlnThr: 1.303 ± 0.038
2.478GlnVal: 2.478 ± 0.051
0.506GlnTrp: 0.506 ± 0.027
0.65GlnTyr: 0.65 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
8.157ArgAla: 8.157 ± 0.111
0.408ArgCys: 0.408 ± 0.021
3.805ArgAsp: 3.805 ± 0.062
4.138ArgGlu: 4.138 ± 0.07
2.371ArgPhe: 2.371 ± 0.053
5.623ArgGly: 5.623 ± 0.086
1.603ArgHis: 1.603 ± 0.048
3.542ArgIle: 3.542 ± 0.065
1.586ArgLys: 1.586 ± 0.046
7.092ArgLeu: 7.092 ± 0.101
1.631ArgMet: 1.631 ± 0.048
1.651ArgAsn: 1.651 ± 0.042
3.777ArgPro: 3.777 ± 0.075
2.173ArgGln: 2.173 ± 0.048
6.486ArgArg: 6.486 ± 0.12
4.698ArgSer: 4.698 ± 0.077
4.99ArgThr: 4.99 ± 0.076
5.441ArgVal: 5.441 ± 0.079
1.141ArgTrp: 1.141 ± 0.04
1.589ArgTyr: 1.589 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
7.177SerAla: 7.177 ± 0.098
0.352SerCys: 0.352 ± 0.019
2.883SerAsp: 2.883 ± 0.055
2.978SerGlu: 2.978 ± 0.057
1.94SerPhe: 1.94 ± 0.043
6.063SerGly: 6.063 ± 0.086
1.023SerHis: 1.023 ± 0.035
2.482SerIle: 2.482 ± 0.056
1.063SerLys: 1.063 ± 0.039
5.553SerLeu: 5.553 ± 0.089
1.318SerMet: 1.318 ± 0.034
1.185SerAsn: 1.185 ± 0.033
3.239SerPro: 3.239 ± 0.065
1.357SerGln: 1.357 ± 0.037
4.03SerArg: 4.03 ± 0.064
3.877SerSer: 3.877 ± 0.08
4.131SerThr: 4.131 ± 0.07
4.829SerVal: 4.829 ± 0.057
0.933SerTrp: 0.933 ± 0.034
1.33SerTyr: 1.33 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
8.013ThrAla: 8.013 ± 0.098
0.324ThrCys: 0.324 ± 0.018
3.605ThrAsp: 3.605 ± 0.071
3.372ThrGlu: 3.372 ± 0.062
1.92ThrPhe: 1.92 ± 0.041
6.397ThrGly: 6.397 ± 0.09
1.208ThrHis: 1.208 ± 0.038
2.565ThrIle: 2.565 ± 0.049
1.119ThrLys: 1.119 ± 0.039
6.258ThrLeu: 6.258 ± 0.096
1.124ThrMet: 1.124 ± 0.037
1.166ThrAsn: 1.166 ± 0.041
4.092ThrPro: 4.092 ± 0.074
1.555ThrGln: 1.555 ± 0.043
3.853ThrArg: 3.853 ± 0.065
3.705ThrSer: 3.705 ± 0.072
4.189ThrThr: 4.189 ± 0.077
5.869ThrVal: 5.869 ± 0.073
0.834ThrTrp: 0.834 ± 0.032
1.343ThrTyr: 1.343 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
10.13ValAla: 10.13 ± 0.111
0.519ValCys: 0.519 ± 0.025
5.525ValAsp: 5.525 ± 0.083
5.057ValGlu: 5.057 ± 0.092
2.761ValPhe: 2.761 ± 0.053
7.188ValGly: 7.188 ± 0.1
1.971ValHis: 1.971 ± 0.049
4.142ValIle: 4.142 ± 0.086
1.549ValLys: 1.549 ± 0.047
10.583ValLeu: 10.583 ± 0.15
1.573ValMet: 1.573 ± 0.047
1.898ValAsn: 1.898 ± 0.05
5.162ValPro: 5.162 ± 0.076
2.506ValGln: 2.506 ± 0.05
6.193ValArg: 6.193 ± 0.096
5.144ValSer: 5.144 ± 0.084
5.603ValThr: 5.603 ± 0.082
9.075ValVal: 9.075 ± 0.121
1.006ValTrp: 1.006 ± 0.034
1.479ValTyr: 1.479 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.42TrpAla: 1.42 ± 0.043
0.104TrpCys: 0.104 ± 0.012
0.786TrpAsp: 0.786 ± 0.034
0.598TrpGlu: 0.598 ± 0.029
0.516TrpPhe: 0.516 ± 0.022
0.961TrpGly: 0.961 ± 0.034
0.326TrpHis: 0.326 ± 0.018
0.618TrpIle: 0.618 ± 0.029
0.288TrpLys: 0.288 ± 0.019
1.621TrpLeu: 1.621 ± 0.045
0.302TrpMet: 0.302 ± 0.023
0.414TrpAsn: 0.414 ± 0.023
0.597TrpPro: 0.597 ± 0.029
0.533TrpGln: 0.533 ± 0.024
0.993TrpArg: 0.993 ± 0.039
0.897TrpSer: 0.897 ± 0.033
0.852TrpThr: 0.852 ± 0.031
1.076TrpVal: 1.076 ± 0.035
0.307TrpTrp: 0.307 ± 0.02
0.301TrpTyr: 0.301 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.321TyrAla: 2.321 ± 0.049
0.164TyrCys: 0.164 ± 0.016
1.251TyrAsp: 1.251 ± 0.04
1.166TyrGlu: 1.166 ± 0.043
0.751TyrPhe: 0.751 ± 0.032
1.955TyrGly: 1.955 ± 0.048
0.317TyrHis: 0.317 ± 0.019
0.597TyrIle: 0.597 ± 0.028
0.333TyrLys: 0.333 ± 0.02
2.248TyrLeu: 2.248 ± 0.055
0.255TyrMet: 0.255 ± 0.018
0.45TyrAsn: 0.45 ± 0.024
1.091TyrPro: 1.091 ± 0.04
0.635TyrGln: 0.635 ± 0.029
1.644TyrArg: 1.644 ± 0.043
1.242TyrSer: 1.242 ± 0.037
1.208TyrThr: 1.208 ± 0.04
1.667TyrVal: 1.667 ± 0.049
0.331TyrTrp: 0.331 ± 0.019
0.471TyrTyr: 0.471 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2792 proteins (870992 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski