Capybara microvirus Cap3_SP_641
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8Z7|A0A4P8W8Z7_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_641 OX=2585485 PE=4 SV=1
MM1 pKa = 7.26 NAVSDD6 pKa = 4.04 EE7 pKa = 4.23 TALRR11 pKa = 11.84 CNDD14 pKa = 3.55 PSLTQQQFKK23 pKa = 10.91 EE24 pKa = 4.06 EE25 pKa = 3.99 SDD27 pKa = 3.35 INNIVDD33 pKa = 4.48 LFMKK37 pKa = 9.77 TGHH40 pKa = 6.95 LPTPVSMPQYY50 pKa = 10.25 VDD52 pKa = 3.3 YY53 pKa = 11.32 EE54 pKa = 4.79 GVFDD58 pKa = 5.12 FQSAMNAVRR67 pKa = 11.84 QADD70 pKa = 4.14 EE71 pKa = 3.91 NFMRR75 pKa = 11.84 MDD77 pKa = 3.41 AKK79 pKa = 10.73 LRR81 pKa = 11.84 ARR83 pKa = 11.84 FHH85 pKa = 6.67 NSPQEE90 pKa = 3.9 FLEE93 pKa = 5.28 FFADD97 pKa = 3.76 PNNTEE102 pKa = 3.67 EE103 pKa = 4.31 AVRR106 pKa = 11.84 LGLAIAKK113 pKa = 9.16 PKK115 pKa = 10.5 EE116 pKa = 3.94 FTSPVVEE123 pKa = 4.2 SAKK126 pKa = 10.83 ADD128 pKa = 3.59 GAASS132 pKa = 3.37
Molecular weight: 14.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.667
IPC2_protein 4.584
IPC_protein 4.469
Toseland 4.317
ProMoST 4.584
Dawson 4.431
Bjellqvist 4.571
Wikipedia 4.304
Rodwell 4.317
Grimsley 4.228
Solomon 4.418
Lehninger 4.38
Nozaki 4.533
DTASelect 4.698
Thurlkill 4.329
EMBOSS 4.329
Sillero 4.584
Patrickios 4.126
IPC_peptide 4.431
IPC2_peptide 4.584
IPC2.peptide.svr19 4.513
Protein with the highest isoelectric point:
>tr|A0A4P8W5D6|A0A4P8W5D6_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_641 OX=2585485 PE=3 SV=1
MM1 pKa = 7.25 CTALWQHH8 pKa = 6.85 PSHH11 pKa = 6.76 GPTKK15 pKa = 10.39 CGQCVEE21 pKa = 4.27 CRR23 pKa = 11.84 LAYY26 pKa = 9.97 SRR28 pKa = 11.84 EE29 pKa = 3.38 WAIRR33 pKa = 11.84 ITHH36 pKa = 5.98 EE37 pKa = 4.0 QQMHH41 pKa = 6.22 KK42 pKa = 10.07 VSCMLNLTYY51 pKa = 10.74 DD52 pKa = 3.92 DD53 pKa = 3.65 EE54 pKa = 4.95 HH55 pKa = 8.01 LPKK58 pKa = 10.38 HH59 pKa = 5.35 GQLHH63 pKa = 6.26 KK64 pKa = 10.88 EE65 pKa = 3.82 HH66 pKa = 6.3 LQKK69 pKa = 10.66 FFKK72 pKa = 10.52 RR73 pKa = 11.84 MRR75 pKa = 11.84 KK76 pKa = 9.02 AGYY79 pKa = 8.83 KK80 pKa = 9.44 FRR82 pKa = 11.84 YY83 pKa = 7.94 VASGEE88 pKa = 4.16 YY89 pKa = 10.49 GDD91 pKa = 4.08 VSRR94 pKa = 11.84 RR95 pKa = 11.84 PHH97 pKa = 4.95 FHH99 pKa = 6.29 IALFGVDD106 pKa = 4.27 FDD108 pKa = 4.72 ADD110 pKa = 3.61 RR111 pKa = 11.84 VPFGRR116 pKa = 11.84 SGGDD120 pKa = 2.79 RR121 pKa = 11.84 TYY123 pKa = 9.44 TSSRR127 pKa = 11.84 VLHH130 pKa = 6.3 LWPFGNHH137 pKa = 7.51 LIGTLNFEE145 pKa = 4.02 SAAYY149 pKa = 8.06 IARR152 pKa = 11.84 YY153 pKa = 8.94 IMKK156 pKa = 10.13 KK157 pKa = 9.76 IKK159 pKa = 10.26 GPNASPVPLFNDD171 pKa = 3.73 DD172 pKa = 3.37 EE173 pKa = 4.57 TGEE176 pKa = 4.28 VVMPNPEE183 pKa = 3.95 FLLVSKK189 pKa = 10.83 GRR191 pKa = 11.84 GKK193 pKa = 10.75 GQGIGGSWFRR203 pKa = 11.84 DD204 pKa = 3.39 YY205 pKa = 11.64 FMTDD209 pKa = 3.26 VFPHH213 pKa = 6.57 AAVITPQRR221 pKa = 11.84 SKK223 pKa = 11.5 APVPRR228 pKa = 11.84 YY229 pKa = 10.04 YY230 pKa = 9.65 KK231 pKa = 10.24 TLLKK235 pKa = 10.7 EE236 pKa = 4.16 LGSDD240 pKa = 4.49 LALDD244 pKa = 3.55 MQFRR248 pKa = 11.84 SSVRR252 pKa = 11.84 ADD254 pKa = 3.29 MEE256 pKa = 4.19 LEE258 pKa = 3.66 RR259 pKa = 11.84 RR260 pKa = 11.84 MLEE263 pKa = 4.02 DD264 pKa = 3.19 QPVRR268 pKa = 11.84 KK269 pKa = 9.02 IARR272 pKa = 11.84 QIVSQSRR279 pKa = 11.84 NNLSKK284 pKa = 10.93 RR285 pKa = 11.84 ILL287 pKa = 3.61
Molecular weight: 33.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.941
IPC2_protein 9.033
IPC_protein 9.004
Toseland 9.633
ProMoST 9.414
Dawson 9.911
Bjellqvist 9.648
Wikipedia 10.087
Rodwell 10.175
Grimsley 9.984
Solomon 9.94
Lehninger 9.897
Nozaki 9.75
DTASelect 9.604
Thurlkill 9.75
EMBOSS 10.072
Sillero 9.853
Patrickios 5.347
IPC_peptide 9.926
IPC2_peptide 8.39
IPC2.peptide.svr19 7.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1330
85
552
266.0
29.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.624 ± 1.97
0.602 ± 0.337
5.564 ± 0.621
3.835 ± 0.925
5.263 ± 0.793
7.594 ± 1.025
2.03 ± 0.779
4.06 ± 0.353
3.985 ± 0.897
8.346 ± 0.524
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.632 ± 0.512
5.94 ± 0.871
5.038 ± 0.699
5.714 ± 0.877
5.414 ± 1.004
7.82 ± 0.687
5.338 ± 0.809
6.692 ± 1.05
0.977 ± 0.284
3.534 ± 0.459
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here