Saccharibacter sp. 17.LH.SD
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1859 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4YWP2|A0A6I4YWP2_9PROT Head protein OS=Saccharibacter sp. 17.LH.SD OX=2689393 GN=GS501_02445 PE=4 SV=1
MM1 pKa = 7.27 ATQPVDD7 pKa = 3.44 EE8 pKa = 5.49 LDD10 pKa = 3.44 YY11 pKa = 10.53 FYY13 pKa = 11.03 QGAVDD18 pKa = 3.9 TSEE21 pKa = 5.18 LDD23 pKa = 4.84 DD24 pKa = 3.94 VLTPNDD30 pKa = 3.66 SKK32 pKa = 11.7 YY33 pKa = 9.54 ITLKK37 pKa = 10.43 PYY39 pKa = 9.17 WRR41 pKa = 11.84 EE42 pKa = 3.65 PTASPDD48 pKa = 3.65 YY49 pKa = 9.07 PTGAVV54 pKa = 3.14
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.77
IPC_protein 3.668
Toseland 3.465
ProMoST 3.884
Dawson 3.681
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.503
Grimsley 3.389
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.795
Patrickios 0.604
IPC_peptide 3.643
IPC2_peptide 3.757
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A6I4YSP3|A0A6I4YSP3_9PROT Uncharacterized protein OS=Saccharibacter sp. 17.LH.SD OX=2689393 GN=GS501_04975 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.11 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.5 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 SATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.85 GRR39 pKa = 11.84 KK40 pKa = 8.87 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1859
0
1859
618472
26
1734
332.7
36.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.63 ± 0.063
0.993 ± 0.017
5.247 ± 0.043
5.643 ± 0.062
3.73 ± 0.035
7.896 ± 0.059
2.93 ± 0.032
5.618 ± 0.041
3.695 ± 0.041
10.421 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.024
3.194 ± 0.04
5.24 ± 0.047
4.016 ± 0.037
6.61 ± 0.051
6.482 ± 0.043
5.581 ± 0.046
6.793 ± 0.059
1.362 ± 0.025
2.511 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here