Canis lupus familiaris (Dog) (Canis familiaris)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45399 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5F4C8E2|A0A5F4C8E2_CANLF Olfactory receptor OS=Canis lupus familiaris OX=9615 GN=OR4A39 PE=3 SV=1
PP1 pKa = 5.79EE2 pKa = 4.36TKK4 pKa = 10.04TEE6 pKa = 3.76AVLNGKK12 pKa = 8.91GLGTGLLYY20 pKa = 9.53ITEE23 pKa = 4.7SCLSWLDD30 pKa = 3.49GSGLGFSLEE39 pKa = 4.09YY40 pKa = 9.04PTISLHH46 pKa = 6.43AVSRR50 pKa = 11.84DD51 pKa = 3.01LNAYY55 pKa = 8.49PRR57 pKa = 11.84EE58 pKa = 4.02HH59 pKa = 7.32LYY61 pKa = 11.69VMVNAKK67 pKa = 10.12FGQEE71 pKa = 4.03SKK73 pKa = 11.01EE74 pKa = 4.13SVAEE78 pKa = 4.02EE79 pKa = 4.62EE80 pKa = 4.52EE81 pKa = 5.18DD82 pKa = 3.96SDD84 pKa = 6.17DD85 pKa = 3.89DD86 pKa = 4.15VEE88 pKa = 5.15PIAEE92 pKa = 4.09FRR94 pKa = 11.84FVPSDD99 pKa = 3.36KK100 pKa = 10.88SALEE104 pKa = 3.9AMFTAMCEE112 pKa = 4.33CQALHH117 pKa = 7.39PDD119 pKa = 4.25PEE121 pKa = 6.21DD122 pKa = 4.16EE123 pKa = 6.53DD124 pKa = 5.1SDD126 pKa = 5.45DD127 pKa = 4.29YY128 pKa = 11.91DD129 pKa = 4.07GEE131 pKa = 4.35EE132 pKa = 4.51YY133 pKa = 10.58DD134 pKa = 5.25VEE136 pKa = 4.24AHH138 pKa = 5.97EE139 pKa = 4.68QGQGDD144 pKa = 3.76IPTFYY149 pKa = 9.69TYY151 pKa = 10.91EE152 pKa = 4.64GGLSHH157 pKa = 7.05LTAEE161 pKa = 4.71GQATLEE167 pKa = 4.16RR168 pKa = 11.84LEE170 pKa = 4.8GMLSQSVSSQYY181 pKa = 11.68NMAGVRR187 pKa = 11.84TEE189 pKa = 4.15DD190 pKa = 3.91SIRR193 pKa = 11.84DD194 pKa = 3.72YY195 pKa = 11.39EE196 pKa = 5.4DD197 pKa = 3.26GMEE200 pKa = 4.14VDD202 pKa = 3.82TTPTVAGQFEE212 pKa = 4.72DD213 pKa = 3.71TDD215 pKa = 3.5VDD217 pKa = 3.87HH218 pKa = 7.41

Molecular weight: 24.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5F4C3Z1|A0A5F4C3Z1_CANLF Dual-specificity kinase OS=Canis lupus familiaris OX=9615 GN=DYRK3 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20654

24745

45399

29901829

10

27097

658.6

73.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.951 ± 0.014

2.176 ± 0.009

4.82 ± 0.008

7.161 ± 0.015

3.555 ± 0.008

6.546 ± 0.017

2.56 ± 0.005

4.447 ± 0.011

5.838 ± 0.014

9.853 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.004

3.653 ± 0.009

6.35 ± 0.021

4.796 ± 0.011

5.761 ± 0.012

8.346 ± 0.015

5.293 ± 0.009

5.926 ± 0.01

1.215 ± 0.004

2.601 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski