[Ruminococcus] lactaris ATCC 29176

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Mediterraneibacter; [Ruminococcus] lactaris

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2706 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5CNM6|B5CNM6_9FIRM Uncharacterized protein OS=[Ruminococcus] lactaris ATCC 29176 OX=471875 GN=RUMLAC_01064 PE=4 SV=1
MM1 pKa = 7.17RR2 pKa = 11.84TIFAEE7 pKa = 4.1YY8 pKa = 10.28NPGRR12 pKa = 11.84NSIDD16 pKa = 3.35VYY18 pKa = 10.65TSVGYY23 pKa = 7.71MLRR26 pKa = 11.84IDD28 pKa = 3.39CWEE31 pKa = 4.04AEE33 pKa = 4.31KK34 pKa = 10.92DD35 pKa = 3.67LKK37 pKa = 8.08TTSGSDD43 pKa = 3.28CALNALAIDD52 pKa = 4.79DD53 pKa = 4.25PLEE56 pKa = 3.93YY57 pKa = 10.7ARR59 pKa = 11.84LYY61 pKa = 11.16LDD63 pKa = 5.06GNLQMWVDD71 pKa = 4.24AEE73 pKa = 4.39DD74 pKa = 3.74SLDD77 pKa = 3.46IFF79 pKa = 4.97

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5CNG2|B5CNG2_9FIRM S4 domain protein OS=[Ruminococcus] lactaris ATCC 29176 OX=471875 GN=RUMLAC_00998 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2706

0

2706

800791

39

2647

295.9

33.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.325 ± 0.049

1.386 ± 0.019

5.634 ± 0.048

8.092 ± 0.067

3.928 ± 0.039

7.166 ± 0.042

1.728 ± 0.022

7.107 ± 0.048

7.295 ± 0.043

8.781 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.099 ± 0.029

4.26 ± 0.042

3.157 ± 0.026

3.307 ± 0.028

4.523 ± 0.044

5.735 ± 0.042

5.506 ± 0.068

6.923 ± 0.04

0.92 ± 0.02

4.128 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski