Vitrella brassicaformis (strain CCMP3155)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Colpodellida; Vitrellaceae; Vitrella; Vitrella brassicaformis

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23030 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G4EEP3|A0A0G4EEP3_VITBC Uncharacterized protein OS=Vitrella brassicaformis (strain CCMP3155) OX=1169540 GN=Vbra_11403 PE=4 SV=1
MM1 pKa = 7.77AARR4 pKa = 11.84PQPGVLYY11 pKa = 10.44RR12 pKa = 11.84VDD14 pKa = 4.55DD15 pKa = 4.39DD16 pKa = 4.3PGVRR20 pKa = 11.84RR21 pKa = 11.84GGDD24 pKa = 3.38LEE26 pKa = 4.47RR27 pKa = 11.84YY28 pKa = 9.08GYY30 pKa = 10.48QRR32 pKa = 11.84TALEE36 pKa = 3.8DD37 pKa = 3.32HH38 pKa = 6.53VRR40 pKa = 11.84FGSQQRR46 pKa = 11.84STWISTSKK54 pKa = 10.73SPSAALYY61 pKa = 10.37YY62 pKa = 10.55GLRR65 pKa = 11.84DD66 pKa = 3.31EE67 pKa = 5.22NIRR70 pKa = 11.84DD71 pKa = 3.4KK72 pKa = 10.55TLLVIRR78 pKa = 11.84PDD80 pKa = 3.78DD81 pKa = 3.93GNRR84 pKa = 11.84GHH86 pKa = 7.12DD87 pKa = 3.45VSGRR91 pKa = 11.84LPDD94 pKa = 3.53QRR96 pKa = 11.84AHH98 pKa = 6.14NFADD102 pKa = 3.47ASEE105 pKa = 4.13EE106 pKa = 4.42VVFDD110 pKa = 3.43SCIPAQCIVDD120 pKa = 4.05RR121 pKa = 11.84VPLSEE126 pKa = 5.83LLDD129 pKa = 3.85EE130 pKa = 5.14LDD132 pKa = 3.79IDD134 pKa = 4.09EE135 pKa = 4.95GDD137 pKa = 3.99ARR139 pKa = 11.84DD140 pKa = 3.62MAQSLGVRR148 pKa = 11.84DD149 pKa = 4.03FRR151 pKa = 11.84EE152 pKa = 4.06AMEE155 pKa = 5.15GYY157 pKa = 8.76YY158 pKa = 9.66QVEE161 pKa = 3.83GRR163 pKa = 11.84MDD165 pKa = 3.51EE166 pKa = 4.59LYY168 pKa = 9.19ATGRR172 pKa = 11.84PVHH175 pKa = 6.76SSTAGDD181 pKa = 3.38GGYY184 pKa = 10.35DD185 pKa = 3.45EE186 pKa = 5.96PSYY189 pKa = 11.62DD190 pKa = 3.43WADD193 pKa = 3.76PPDD196 pKa = 4.11GAEE199 pKa = 3.94SPVYY203 pKa = 9.89RR204 pKa = 11.84VGGFTVLDD212 pKa = 4.42DD213 pKa = 4.85EE214 pKa = 6.11PDD216 pKa = 3.32DD217 pKa = 3.99TYY219 pKa = 11.89EE220 pKa = 3.83NDD222 pKa = 3.56YY223 pKa = 10.74EE224 pKa = 4.61NEE226 pKa = 4.09VAHH229 pKa = 6.72TGGLWMVDD237 pKa = 3.69DD238 pKa = 5.27DD239 pKa = 5.34QDD241 pKa = 3.69PAANGPYY248 pKa = 9.73DD249 pKa = 4.8DD250 pKa = 4.36EE251 pKa = 5.25PSYY254 pKa = 11.06VGHH257 pKa = 6.99IGGFYY262 pKa = 10.28IVDD265 pKa = 3.83EE266 pKa = 4.55QPAEE270 pKa = 3.92ALSAGRR276 pKa = 11.84SYY278 pKa = 11.65GDD280 pKa = 3.37QDD282 pKa = 4.21SEE284 pKa = 4.45DD285 pKa = 4.23DD286 pKa = 3.7EE287 pKa = 5.91GEE289 pKa = 4.0EE290 pKa = 4.57SEE292 pKa = 4.8VNSGSHH298 pKa = 6.87DD299 pKa = 3.93DD300 pKa = 4.53GYY302 pKa = 11.8GSYY305 pKa = 10.47EE306 pKa = 3.98VVEE309 pKa = 4.18EE310 pKa = 5.12HH311 pKa = 7.01VDD313 pKa = 3.58QSDD316 pKa = 3.45AAAAAADD323 pKa = 3.92GAEE326 pKa = 4.37EE327 pKa = 4.65GGGDD331 pKa = 3.79PEE333 pKa = 4.2YY334 pKa = 10.87DD335 pKa = 3.33YY336 pKa = 11.88GGNDD340 pKa = 3.12GDD342 pKa = 4.83AYY344 pKa = 10.7GAADD348 pKa = 4.32DD349 pKa = 4.98GGYY352 pKa = 10.12SDD354 pKa = 4.73GYY356 pKa = 10.47GWGSEE361 pKa = 4.38GGDD364 pKa = 3.82SDD366 pKa = 3.58WSAA369 pKa = 3.46

Molecular weight: 40.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G4EN05|A0A0G4EN05_VITBC Uncharacterized protein OS=Vitrella brassicaformis (strain CCMP3155) OX=1169540 GN=Vbra_22906 PE=4 SV=1
MM1 pKa = 7.06ATSRR5 pKa = 11.84YY6 pKa = 10.19ARR8 pKa = 11.84MLQLWWLTLPHH19 pKa = 5.58RR20 pKa = 11.84QKK22 pKa = 10.48RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84MGSSRR30 pKa = 11.84PLPQFCRR37 pKa = 11.84VVPPPLPRR45 pKa = 11.84HH46 pKa = 5.86HH47 pKa = 6.95SRR49 pKa = 11.84PASRR53 pKa = 11.84RR54 pKa = 11.84HH55 pKa = 5.23RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84SRR60 pKa = 11.84QTRR63 pKa = 11.84QNLSVRR69 pKa = 11.84RR70 pKa = 11.84WII72 pKa = 4.1

Molecular weight: 8.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23030

0

23030

11959242

19

9609

519.3

57.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.602 ± 0.019

1.701 ± 0.009

5.942 ± 0.012

6.353 ± 0.015

3.172 ± 0.011

7.649 ± 0.017

2.806 ± 0.008

3.683 ± 0.011

4.169 ± 0.016

8.856 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.313 ± 0.006

2.552 ± 0.009

6.776 ± 0.02

4.362 ± 0.013

7.073 ± 0.017

7.455 ± 0.015

5.502 ± 0.013

6.459 ± 0.013

1.359 ± 0.006

2.183 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski