Stenotrophomonas koreensis
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2597 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R0BSN2|A0A0R0BSN2_9GAMM DNA glycosylase OS=Stenotrophomonas koreensis OX=266128 GN=ABB25_02295 PE=4 SV=1
MM1 pKa = 7.58 LSEE4 pKa = 5.95 LITLLTCDD12 pKa = 4.47 DD13 pKa = 4.33 LQGEE17 pKa = 4.3 ALADD21 pKa = 3.62 CVSRR25 pKa = 11.84 ITAAPMDD32 pKa = 4.84 PEE34 pKa = 4.85 LDD36 pKa = 3.66 WIEE39 pKa = 4.49 GDD41 pKa = 3.74 SQAEE45 pKa = 4.03 YY46 pKa = 9.53 MQFAVLAEE54 pKa = 4.35 LGDD57 pKa = 4.05 YY58 pKa = 10.46 FAMGQQPEE66 pKa = 4.16 QLHH69 pKa = 5.78 EE70 pKa = 4.8 EE71 pKa = 3.9 ISEE74 pKa = 4.1 QFAAPLAPLPQADD87 pKa = 4.13 MSLGAYY93 pKa = 7.28 FQWLDD98 pKa = 3.26 AEE100 pKa = 4.55 LAARR104 pKa = 11.84 PTPYY108 pKa = 10.52 QLLEE112 pKa = 3.85 WGNFFDD118 pKa = 5.78 DD119 pKa = 3.48 NLYY122 pKa = 9.29 TFIVRR127 pKa = 11.84 RR128 pKa = 11.84 ADD130 pKa = 3.4 TPRR133 pKa = 11.84 ILSLAGQLGLQVEE146 pKa = 4.75 PANTAGG152 pKa = 3.69
Molecular weight: 16.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.427
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.541
Rodwell 3.452
Grimsley 3.338
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.91
Thurlkill 3.478
EMBOSS 3.541
Sillero 3.745
Patrickios 0.846
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A0R0BU21|A0A0R0BU21_9GAMM Histidine kinase OS=Stenotrophomonas koreensis OX=266128 GN=ABB25_00515 PE=4 SV=1
MM1 pKa = 7.22 MLSIQQGLQEE11 pKa = 4.48 EE12 pKa = 5.28 GVVVPMTKK20 pKa = 9.32 LCRR23 pKa = 11.84 WFGVARR29 pKa = 11.84 RR30 pKa = 11.84 TVYY33 pKa = 10.37 YY34 pKa = 10.31 HH35 pKa = 5.14 QTKK38 pKa = 9.55 QPARR42 pKa = 11.84 VRR44 pKa = 11.84 PEE46 pKa = 3.25 LAEE49 pKa = 4.68 PIKK52 pKa = 10.59 QLIEE56 pKa = 3.85 QEE58 pKa = 4.06 PSFGYY63 pKa = 8.74 RR64 pKa = 11.84 TVAALLGMNKK74 pKa = 8.28 NTVQRR79 pKa = 11.84 IFQLKK84 pKa = 7.71 GWQVRR89 pKa = 11.84 KK90 pKa = 9.9 RR91 pKa = 11.84 PIGRR95 pKa = 11.84 RR96 pKa = 11.84 PRR98 pKa = 11.84 IQALPSVAAAPNQRR112 pKa = 11.84 WATDD116 pKa = 3.44 LCRR119 pKa = 11.84 VWGGKK124 pKa = 9.77 DD125 pKa = 2.94 GWLSLALVIDD135 pKa = 3.83 CHH137 pKa = 6.05 TRR139 pKa = 11.84 QLLGWHH145 pKa = 7.13 LSRR148 pKa = 11.84 TGKK151 pKa = 10.66 ASTASAALEE160 pKa = 3.78 QALITRR166 pKa = 11.84 FGTLGRR172 pKa = 11.84 VSEE175 pKa = 4.24 PFLLRR180 pKa = 11.84 SDD182 pKa = 3.61 NGLVFTSRR190 pKa = 11.84 DD191 pKa = 3.27 YY192 pKa = 10.56 TRR194 pKa = 11.84 LVRR197 pKa = 11.84 SYY199 pKa = 11.26 GLQQEE204 pKa = 5.57 FITPHH209 pKa = 6.55 CPQQNGMVEE218 pKa = 3.99 RR219 pKa = 11.84 VIRR222 pKa = 11.84 TLKK225 pKa = 7.71 EE226 pKa = 3.85 QCVHH230 pKa = 5.07 RR231 pKa = 11.84 HH232 pKa = 4.52 RR233 pKa = 11.84 FEE235 pKa = 4.4 SQVHH239 pKa = 5.97 ALRR242 pKa = 11.84 VIGDD246 pKa = 4.08 WIAFYY251 pKa = 10.9 NRR253 pKa = 11.84 QRR255 pKa = 11.84 PHH257 pKa = 5.78 QALKK261 pKa = 10.74 MMTPDD266 pKa = 3.07 AAYY269 pKa = 10.12 AATLTAA275 pKa = 5.1
Molecular weight: 31.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.692
IPC_protein 10.628
Toseland 10.672
ProMoST 10.452
Dawson 10.789
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 10.906
Grimsley 10.833
Solomon 10.921
Lehninger 10.877
Nozaki 10.672
DTASelect 10.526
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.716
Patrickios 10.599
IPC_peptide 10.921
IPC2_peptide 9.736
IPC2.peptide.svr19 8.508
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2597
0
2597
842727
33
1764
324.5
35.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.444 ± 0.057
0.882 ± 0.015
5.444 ± 0.034
4.976 ± 0.046
3.16 ± 0.027
8.557 ± 0.049
2.28 ± 0.028
4.203 ± 0.029
2.467 ± 0.043
11.475 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.149 ± 0.021
2.609 ± 0.029
5.262 ± 0.037
4.921 ± 0.037
7.203 ± 0.04
5.204 ± 0.034
4.568 ± 0.036
7.357 ± 0.039
1.602 ± 0.026
2.237 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here