Limnohabitans sp. 103DPR2

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Limnohabitans; unclassified Limnohabitans

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2804 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0MHY7|A0A0P0MHY7_9BURK HemY_N domain-containing protein OS=Limnohabitans sp. 103DPR2 OX=1678129 GN=L103DPR2_02444 PE=4 SV=1
MM1 pKa = 7.53PIKK4 pKa = 10.79YY5 pKa = 7.64GTNGNDD11 pKa = 3.02NPLRR15 pKa = 11.84GTSGNDD21 pKa = 3.04SLYY24 pKa = 11.36GLAGDD29 pKa = 5.39DD30 pKa = 5.35FILTEE35 pKa = 4.61DD36 pKa = 3.84GEE38 pKa = 5.11DD39 pKa = 3.62YY40 pKa = 11.54VEE42 pKa = 5.77AGDD45 pKa = 5.92GDD47 pKa = 4.77DD48 pKa = 4.64EE49 pKa = 5.16VNGYY53 pKa = 10.78DD54 pKa = 4.14GVGGSYY60 pKa = 9.69TYY62 pKa = 11.24YY63 pKa = 9.86PVAGIKK69 pKa = 9.01TIHH72 pKa = 6.52GGNGNDD78 pKa = 3.51FLVGGSAGDD87 pKa = 3.48VLYY90 pKa = 10.99GDD92 pKa = 4.61EE93 pKa = 6.01GNDD96 pKa = 3.26QLYY99 pKa = 10.81GRR101 pKa = 11.84GGNDD105 pKa = 2.9ILSGGPGADD114 pKa = 3.75YY115 pKa = 11.39LNGGPGDD122 pKa = 3.62DD123 pKa = 3.74TYY125 pKa = 11.67YY126 pKa = 11.11VSDD129 pKa = 3.13IHH131 pKa = 8.09DD132 pKa = 4.2VIEE135 pKa = 4.8DD136 pKa = 3.49VSGTDD141 pKa = 3.06TAYY144 pKa = 10.84VATSFVKK151 pKa = 10.35IPSSIEE157 pKa = 3.47KK158 pKa = 10.46VIYY161 pKa = 9.98TDD163 pKa = 4.38GAQSLPYY170 pKa = 9.53WVDD173 pKa = 2.85ALLPDD178 pKa = 4.19EE179 pKa = 4.64AAGNAFEE186 pKa = 4.79SLLGSAHH193 pKa = 6.25TYY195 pKa = 10.19FYY197 pKa = 10.36TFPTSLPTYY206 pKa = 7.41DD207 pKa = 3.36TNYY210 pKa = 10.23SHH212 pKa = 7.23GLGFKK217 pKa = 10.33PFTSTQMARR226 pKa = 11.84AEE228 pKa = 3.99AALSIVSSVIDD239 pKa = 3.08VHH241 pKa = 6.31FQKK244 pKa = 9.98TNNPGVLNTFVFANNDD260 pKa = 3.51QPSSAGSGNFPSDD273 pKa = 3.5YY274 pKa = 10.34MIGSDD279 pKa = 4.78LYY281 pKa = 11.29FDD283 pKa = 4.14NSSLNAAFADD293 pKa = 4.05RR294 pKa = 11.84TYY296 pKa = 11.55GALTLIHH303 pKa = 6.93EE304 pKa = 4.83IGHH307 pKa = 6.01GLGLEE312 pKa = 4.45HH313 pKa = 7.29PFSHH317 pKa = 6.42AQAGSSSVSDD327 pKa = 3.7PPYY330 pKa = 9.85LTGTEE335 pKa = 4.59EE336 pKa = 4.0STAWTVMSYY345 pKa = 11.42NDD347 pKa = 4.81APAQYY352 pKa = 9.53YY353 pKa = 10.82LSFSPLDD360 pKa = 3.52IAALQYY366 pKa = 10.56IYY368 pKa = 10.89GPSKK372 pKa = 9.91TSRR375 pKa = 11.84TGNDD379 pKa = 3.2TYY381 pKa = 11.05KK382 pKa = 10.9VSATEE387 pKa = 5.0PNFIWDD393 pKa = 3.64GAGVDD398 pKa = 4.03TLDD401 pKa = 5.21ASNLNQGSTLYY412 pKa = 8.75LTPGYY417 pKa = 8.95WGYY420 pKa = 11.43VGNNKK425 pKa = 8.3ATNITAAGQVTVNFGSAIEE444 pKa = 4.15NLTGSSFADD453 pKa = 2.99KK454 pKa = 10.9LYY456 pKa = 11.12GNEE459 pKa = 5.2LGNQMSGGMGNDD471 pKa = 3.32WLEE474 pKa = 3.86GWAGDD479 pKa = 3.74DD480 pKa = 3.94TLVGGQGDD488 pKa = 4.04DD489 pKa = 3.55QLQGGSGIDD498 pKa = 3.18TALFGGAYY506 pKa = 10.12ASYY509 pKa = 9.23TFEE512 pKa = 4.28NTSSTFSVKK521 pKa = 10.3DD522 pKa = 3.15KK523 pKa = 10.65RR524 pKa = 11.84ANADD528 pKa = 3.75GIDD531 pKa = 3.45VLTSVEE537 pKa = 4.03RR538 pKa = 11.84LKK540 pKa = 11.3FSDD543 pKa = 3.27KK544 pKa = 10.94SVAIDD549 pKa = 3.61LDD551 pKa = 4.21GNAGIVVKK559 pKa = 10.8VIGAVLGSDD568 pKa = 3.34AVKK571 pKa = 9.89TPGIVGTGLRR581 pKa = 11.84YY582 pKa = 9.9VDD584 pKa = 4.25NGMSYY589 pKa = 11.7ADD591 pKa = 4.58LGLTALNAVGAMTPDD606 pKa = 4.85AIVSTLWRR614 pKa = 11.84NVVGSIASATEE625 pKa = 3.47KK626 pKa = 10.94APYY629 pKa = 10.62LKK631 pKa = 10.03MLADD635 pKa = 3.67GTKK638 pKa = 10.33PGDD641 pKa = 3.76LVVLAGDD648 pKa = 4.65FSLNMNKK655 pKa = 9.63IGLMGLAQTGIEE667 pKa = 4.32FSS669 pKa = 3.51

Molecular weight: 70.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0MCP3|A0A0P0MCP3_9BURK Major Facilitator Superfamily protein OS=Limnohabitans sp. 103DPR2 OX=1678129 GN=L103DPR2_00246 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2804

0

2804

922556

31

4090

329.0

35.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.25 ± 0.058

0.964 ± 0.015

4.99 ± 0.034

5.213 ± 0.042

3.757 ± 0.028

7.89 ± 0.04

2.352 ± 0.026

4.886 ± 0.033

4.696 ± 0.039

10.384 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.934 ± 0.027

3.347 ± 0.033

4.851 ± 0.031

4.591 ± 0.036

5.161 ± 0.041

5.956 ± 0.043

5.397 ± 0.056

7.547 ± 0.031

1.533 ± 0.025

2.3 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski