Gokushovirinae Bog5712_52

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0G2UK10|A0A0G2UK10_9VIRU Major capsid protein VP1 OS=Gokushovirinae Bog5712_52 OX=1655649 PE=3 SV=1
MM1 pKa = 7.42SSKK4 pKa = 10.1IVPPFLRR11 pKa = 11.84TPYY14 pKa = 10.62NYY16 pKa = 10.57DD17 pKa = 3.06RR18 pKa = 11.84DD19 pKa = 4.06AVSDD23 pKa = 3.76EE24 pKa = 4.14TGLRR28 pKa = 11.84CEE30 pKa = 4.4DD31 pKa = 3.0VSLAVQDD38 pKa = 5.26ARR40 pKa = 11.84DD41 pKa = 3.53EE42 pKa = 4.4CDD44 pKa = 2.77INTIVKK50 pKa = 10.13NFGLTGSLPDD60 pKa = 3.49VVVPPSYY67 pKa = 11.38GDD69 pKa = 3.41YY70 pKa = 10.66TGVSDD75 pKa = 5.01FQSAMNAVRR84 pKa = 11.84QAAEE88 pKa = 4.37DD89 pKa = 4.39FMALPAQVRR98 pKa = 11.84SRR100 pKa = 11.84FEE102 pKa = 4.13NDD104 pKa = 2.8PQRR107 pKa = 11.84YY108 pKa = 9.18LEE110 pKa = 4.8FFTDD114 pKa = 4.35PKK116 pKa = 10.97NQDD119 pKa = 2.74EE120 pKa = 4.9AIRR123 pKa = 11.84LGLATRR129 pKa = 11.84SPEE132 pKa = 4.13DD133 pKa = 3.39PSAILARR140 pKa = 11.84GVASTPPAAPVAVAGQPAAPAGAASPPAGGG170 pKa = 3.62

Molecular weight: 18.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0G2UEC1|A0A0G2UEC1_9VIRU DNA pilot protein VP2 OS=Gokushovirinae Bog5712_52 OX=1655649 PE=4 SV=1
VV1 pKa = 6.27TPALLCVTFWARR13 pKa = 11.84VHH15 pKa = 6.67PGPFFNGGFMRR26 pKa = 11.84PLKK29 pKa = 10.07RR30 pKa = 11.84KK31 pKa = 8.89HH32 pKa = 5.29VSKK35 pKa = 10.86GKK37 pKa = 8.75SAKK40 pKa = 7.79TFRR43 pKa = 11.84KK44 pKa = 9.62HH45 pKa = 4.67SHH47 pKa = 6.12HH48 pKa = 6.89SKK50 pKa = 10.68APNVAQAPMRR60 pKa = 11.84GGWRR64 pKa = 11.84LL65 pKa = 3.13

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1464

65

536

244.0

26.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.954 ± 2.691

1.093 ± 0.423

5.26 ± 0.539

3.279 ± 0.585

4.986 ± 0.983

9.016 ± 0.817

1.776 ± 0.513

3.893 ± 0.781

3.21 ± 0.819

8.197 ± 0.529

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.563

3.825 ± 0.787

6.148 ± 0.878

4.918 ± 1.077

6.489 ± 1.204

7.104 ± 0.789

6.011 ± 1.413

5.874 ± 0.992

1.298 ± 0.285

3.415 ± 0.566
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski