Paenibacillus phage Tripp
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N9SJW5|A0A0N9SJW5_9CAUD Single-stranded DNA-binding protein OS=Paenibacillus phage Tripp OX=1718161 GN=TRIPP_57 PE=4 SV=1
MM1 pKa = 7.88 PEE3 pKa = 4.73 LDD5 pKa = 3.53 RR6 pKa = 11.84 FDD8 pKa = 4.75 YY9 pKa = 11.06 DD10 pKa = 3.31 GSEE13 pKa = 4.2 PDD15 pKa = 3.8 VVATCASCDD24 pKa = 3.6 GEE26 pKa = 4.75 VYY28 pKa = 10.71 EE29 pKa = 5.47 GDD31 pKa = 3.86 DD32 pKa = 3.77 VVLTTEE38 pKa = 4.04 GDD40 pKa = 3.6 IVHH43 pKa = 6.53 EE44 pKa = 4.26 EE45 pKa = 4.11 CFAAFARR52 pKa = 11.84 EE53 pKa = 3.83 IYY55 pKa = 10.28 RR56 pKa = 11.84 NVSGTIDD63 pKa = 3.32 ANGRR67 pKa = 11.84 III69 pKa = 4.67
Molecular weight: 7.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.528
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|A0A0N9SJU7|A0A0N9SJU7_9CAUD Uncharacterized protein OS=Paenibacillus phage Tripp OX=1718161 GN=TRIPP_50 PE=4 SV=1
MM1 pKa = 7.65 KK2 pKa = 10.7 SNIFYY7 pKa = 10.71 EE8 pKa = 4.55 VVGKK12 pKa = 9.85 NIRR15 pKa = 11.84 KK16 pKa = 7.99 YY17 pKa = 10.65 RR18 pKa = 11.84 EE19 pKa = 3.38 IRR21 pKa = 11.84 GYY23 pKa = 10.41 SLQLLGDD30 pKa = 3.75 RR31 pKa = 11.84 VGLTKK36 pKa = 10.74 KK37 pKa = 10.34 LFNDD41 pKa = 3.06 MRR43 pKa = 11.84 AVILRR48 pKa = 11.84 LTT50 pKa = 3.67
Molecular weight: 5.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 9.882
IPC_protein 10.511
Toseland 10.628
ProMoST 10.277
Dawson 10.76
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.818
Solomon 10.833
Lehninger 10.804
Nozaki 10.599
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.672
Patrickios 10.906
IPC_peptide 10.833
IPC2_peptide 9.151
IPC2.peptide.svr19 8.55
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
92
0
92
16473
24
962
179.1
20.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.062 ± 0.268
1.014 ± 0.116
6.817 ± 0.192
7.989 ± 0.331
3.569 ± 0.149
6.823 ± 0.309
1.688 ± 0.119
6.198 ± 0.186
7.291 ± 0.336
8.317 ± 0.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.477 ± 0.14
3.715 ± 0.164
3.466 ± 0.197
3.072 ± 0.19
6.611 ± 0.256
5.111 ± 0.19
5.682 ± 0.227
6.52 ± 0.247
1.39 ± 0.131
4.189 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here