Amino acid dipepetide frequency for Paenibacillus phage Tripp

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.285AlaAla: 7.285 ± 0.751
0.911AlaCys: 0.911 ± 0.233
5.1AlaAsp: 5.1 ± 0.583
7.224AlaGlu: 7.224 ± 0.699
2.61AlaPhe: 2.61 ± 0.367
5.221AlaGly: 5.221 ± 0.671
1.032AlaHis: 1.032 ± 0.232
4.735AlaIle: 4.735 ± 0.628
5.828AlaLys: 5.828 ± 0.709
7.467AlaLeu: 7.467 ± 0.552
2.368AlaMet: 2.368 ± 0.357
3.825AlaAsn: 3.825 ± 0.536
2.186AlaPro: 2.186 ± 0.375
2.489AlaGln: 2.489 ± 0.44
4.25AlaArg: 4.25 ± 0.637
4.675AlaSer: 4.675 ± 0.41
4.068AlaThr: 4.068 ± 0.564
5.464AlaVal: 5.464 ± 0.659
1.032AlaTrp: 1.032 ± 0.341
2.853AlaTyr: 2.853 ± 0.46
0.0AlaXaa: 0.0 ± 0.0
Cys
0.85CysAla: 0.85 ± 0.226
0.061CysCys: 0.061 ± 0.061
0.729CysAsp: 0.729 ± 0.196
0.486CysGlu: 0.486 ± 0.191
0.425CysPhe: 0.425 ± 0.143
0.971CysGly: 0.971 ± 0.269
0.364CysHis: 0.364 ± 0.132
0.425CysIle: 0.425 ± 0.153
0.789CysLys: 0.789 ± 0.207
0.789CysLeu: 0.789 ± 0.244
0.121CysMet: 0.121 ± 0.08
0.182CysAsn: 0.182 ± 0.105
0.668CysPro: 0.668 ± 0.177
0.182CysGln: 0.182 ± 0.101
0.971CysArg: 0.971 ± 0.244
0.607CysSer: 0.607 ± 0.163
0.243CysThr: 0.243 ± 0.115
0.486CysVal: 0.486 ± 0.184
0.304CysTrp: 0.304 ± 0.114
0.486CysTyr: 0.486 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
4.857AspAla: 4.857 ± 0.634
0.729AspCys: 0.729 ± 0.193
5.707AspAsp: 5.707 ± 0.639
6.132AspGlu: 6.132 ± 0.634
3.278AspPhe: 3.278 ± 0.433
5.16AspGly: 5.16 ± 0.611
1.396AspHis: 1.396 ± 0.3
5.282AspIle: 5.282 ± 0.605
2.61AspLys: 2.61 ± 0.472
5.1AspLeu: 5.1 ± 0.5
1.761AspMet: 1.761 ± 0.317
2.307AspAsn: 2.307 ± 0.384
2.307AspPro: 2.307 ± 0.319
1.578AspGln: 1.578 ± 0.283
3.643AspArg: 3.643 ± 0.398
3.4AspSer: 3.4 ± 0.379
3.46AspThr: 3.46 ± 0.446
5.889AspVal: 5.889 ± 0.597
1.336AspTrp: 1.336 ± 0.257
2.246AspTyr: 2.246 ± 0.399
0.0AspXaa: 0.0 ± 0.0
Glu
6.132GluAla: 6.132 ± 0.656
0.546GluCys: 0.546 ± 0.257
4.068GluAsp: 4.068 ± 0.486
6.314GluGlu: 6.314 ± 0.615
2.55GluPhe: 2.55 ± 0.457
4.25GluGly: 4.25 ± 0.635
1.093GluHis: 1.093 ± 0.253
5.282GluIle: 5.282 ± 0.583
6.314GluLys: 6.314 ± 0.814
8.439GluLeu: 8.439 ± 0.811
2.428GluMet: 2.428 ± 0.382
2.61GluAsn: 2.61 ± 0.436
2.428GluPro: 2.428 ± 0.494
3.4GluGln: 3.4 ± 0.521
5.282GluArg: 5.282 ± 0.541
3.885GluSer: 3.885 ± 0.534
4.25GluThr: 4.25 ± 0.541
5.1GluVal: 5.1 ± 0.553
1.153GluTrp: 1.153 ± 0.343
4.371GluTyr: 4.371 ± 0.527
0.0GluXaa: 0.0 ± 0.0
Phe
2.793PheAla: 2.793 ± 0.421
0.182PheCys: 0.182 ± 0.109
2.671PheAsp: 2.671 ± 0.395
2.489PheGlu: 2.489 ± 0.483
1.396PhePhe: 1.396 ± 0.289
2.428PheGly: 2.428 ± 0.402
0.729PheHis: 0.729 ± 0.236
2.428PheIle: 2.428 ± 0.343
2.489PheLys: 2.489 ± 0.321
2.125PheLeu: 2.125 ± 0.376
0.971PheMet: 0.971 ± 0.241
2.064PheAsn: 2.064 ± 0.389
1.093PhePro: 1.093 ± 0.336
0.607PheGln: 0.607 ± 0.17
2.125PheArg: 2.125 ± 0.352
1.821PheSer: 1.821 ± 0.294
3.096PheThr: 3.096 ± 0.319
2.368PheVal: 2.368 ± 0.321
0.243PheTrp: 0.243 ± 0.113
1.578PheTyr: 1.578 ± 0.341
0.0PheXaa: 0.0 ± 0.0
Gly
4.189GlyAla: 4.189 ± 0.645
0.789GlyCys: 0.789 ± 0.259
4.25GlyAsp: 4.25 ± 0.521
4.735GlyGlu: 4.735 ± 0.608
2.428GlyPhe: 2.428 ± 0.465
5.403GlyGly: 5.403 ± 0.614
0.85GlyHis: 0.85 ± 0.202
5.1GlyIle: 5.1 ± 0.477
6.132GlyLys: 6.132 ± 0.579
5.707GlyLeu: 5.707 ± 0.593
1.7GlyMet: 1.7 ± 0.455
2.914GlyAsn: 2.914 ± 0.408
1.761GlyPro: 1.761 ± 0.275
1.943GlyGln: 1.943 ± 0.399
4.432GlyArg: 4.432 ± 0.595
2.489GlySer: 2.489 ± 0.42
4.128GlyThr: 4.128 ± 0.452
4.978GlyVal: 4.978 ± 0.608
1.093GlyTrp: 1.093 ± 0.27
3.218GlyTyr: 3.218 ± 0.432
0.0GlyXaa: 0.0 ± 0.0
His
1.7HisAla: 1.7 ± 0.316
0.304HisCys: 0.304 ± 0.118
1.093HisAsp: 1.093 ± 0.223
1.275HisGlu: 1.275 ± 0.305
0.546HisPhe: 0.546 ± 0.217
1.457HisGly: 1.457 ± 0.312
0.243HisHis: 0.243 ± 0.166
0.911HisIle: 0.911 ± 0.249
0.85HisLys: 0.85 ± 0.225
1.275HisLeu: 1.275 ± 0.28
0.486HisMet: 0.486 ± 0.162
0.486HisAsn: 0.486 ± 0.171
0.789HisPro: 0.789 ± 0.2
0.668HisGln: 0.668 ± 0.18
1.093HisArg: 1.093 ± 0.287
0.789HisSer: 0.789 ± 0.236
1.153HisThr: 1.153 ± 0.327
0.729HisVal: 0.729 ± 0.243
0.304HisTrp: 0.304 ± 0.165
0.729HisTyr: 0.729 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
4.735IleAla: 4.735 ± 0.562
0.85IleCys: 0.85 ± 0.309
5.646IleAsp: 5.646 ± 0.575
4.917IleGlu: 4.917 ± 0.422
1.578IlePhe: 1.578 ± 0.317
4.068IleGly: 4.068 ± 0.547
1.032IleHis: 1.032 ± 0.257
3.643IleIle: 3.643 ± 0.412
4.857IleLys: 4.857 ± 0.554
3.764IleLeu: 3.764 ± 0.465
1.153IleMet: 1.153 ± 0.254
2.428IleAsn: 2.428 ± 0.38
2.61IlePro: 2.61 ± 0.452
1.578IleGln: 1.578 ± 0.309
4.978IleArg: 4.978 ± 0.528
3.339IleSer: 3.339 ± 0.501
3.339IleThr: 3.339 ± 0.465
4.128IleVal: 4.128 ± 0.513
0.729IleTrp: 0.729 ± 0.229
2.61IleTyr: 2.61 ± 0.499
0.0IleXaa: 0.0 ± 0.0
Lys
6.071LysAla: 6.071 ± 0.679
0.425LysCys: 0.425 ± 0.186
4.432LysAsp: 4.432 ± 0.514
5.707LysGlu: 5.707 ± 0.86
2.489LysPhe: 2.489 ± 0.408
4.128LysGly: 4.128 ± 0.555
1.093LysHis: 1.093 ± 0.219
4.735LysIle: 4.735 ± 0.628
6.01LysLys: 6.01 ± 0.72
6.132LysLeu: 6.132 ± 0.681
1.578LysMet: 1.578 ± 0.349
2.125LysAsn: 2.125 ± 0.365
2.186LysPro: 2.186 ± 0.386
2.489LysGln: 2.489 ± 0.39
6.071LysArg: 6.071 ± 0.613
3.582LysSer: 3.582 ± 0.415
5.16LysThr: 5.16 ± 0.586
3.825LysVal: 3.825 ± 0.453
1.214LysTrp: 1.214 ± 0.236
3.46LysTyr: 3.46 ± 0.553
0.0LysXaa: 0.0 ± 0.0
Leu
7.953LeuAla: 7.953 ± 0.837
1.032LeuCys: 1.032 ± 0.279
6.496LeuAsp: 6.496 ± 0.643
6.132LeuGlu: 6.132 ± 0.627
2.368LeuPhe: 2.368 ± 0.352
4.371LeuGly: 4.371 ± 0.538
1.275LeuHis: 1.275 ± 0.312
5.221LeuIle: 5.221 ± 0.594
5.039LeuLys: 5.039 ± 0.651
6.071LeuLeu: 6.071 ± 0.624
1.639LeuMet: 1.639 ± 0.31
2.489LeuAsn: 2.489 ± 0.441
3.946LeuPro: 3.946 ± 0.537
2.61LeuGln: 2.61 ± 0.368
6.374LeuArg: 6.374 ± 0.575
5.889LeuSer: 5.889 ± 0.469
5.464LeuThr: 5.464 ± 0.556
4.432LeuVal: 4.432 ± 0.483
1.093LeuTrp: 1.093 ± 0.355
3.278LeuTyr: 3.278 ± 0.513
0.0LeuXaa: 0.0 ± 0.0
Met
1.761MetAla: 1.761 ± 0.286
0.121MetCys: 0.121 ± 0.094
1.396MetAsp: 1.396 ± 0.264
1.639MetGlu: 1.639 ± 0.267
1.093MetPhe: 1.093 ± 0.222
1.457MetGly: 1.457 ± 0.348
0.243MetHis: 0.243 ± 0.12
0.971MetIle: 0.971 ± 0.239
1.943MetLys: 1.943 ± 0.377
2.428MetLeu: 2.428 ± 0.467
0.729MetMet: 0.729 ± 0.239
1.457MetAsn: 1.457 ± 0.312
1.093MetPro: 1.093 ± 0.236
0.546MetGln: 0.546 ± 0.182
2.307MetArg: 2.307 ± 0.375
1.821MetSer: 1.821 ± 0.327
1.578MetThr: 1.578 ± 0.298
1.336MetVal: 1.336 ± 0.274
0.121MetTrp: 0.121 ± 0.086
0.729MetTyr: 0.729 ± 0.224
0.0MetXaa: 0.0 ± 0.0
Asn
2.793AsnAla: 2.793 ± 0.37
0.425AsnCys: 0.425 ± 0.14
2.914AsnAsp: 2.914 ± 0.505
2.975AsnGlu: 2.975 ± 0.386
1.457AsnPhe: 1.457 ± 0.288
3.946AsnGly: 3.946 ± 0.631
0.668AsnHis: 0.668 ± 0.17
1.457AsnIle: 1.457 ± 0.311
2.003AsnLys: 2.003 ± 0.354
2.368AsnLeu: 2.368 ± 0.435
0.607AsnMet: 0.607 ± 0.217
1.093AsnAsn: 1.093 ± 0.225
2.246AsnPro: 2.246 ± 0.477
1.214AsnGln: 1.214 ± 0.298
2.853AsnArg: 2.853 ± 0.483
1.821AsnSer: 1.821 ± 0.353
1.761AsnThr: 1.761 ± 0.387
2.732AsnVal: 2.732 ± 0.419
0.546AsnTrp: 0.546 ± 0.208
1.275AsnTyr: 1.275 ± 0.321
0.0AsnXaa: 0.0 ± 0.0
Pro
2.914ProAla: 2.914 ± 0.363
0.304ProCys: 0.304 ± 0.132
3.096ProAsp: 3.096 ± 0.386
3.218ProGlu: 3.218 ± 0.483
1.032ProPhe: 1.032 ± 0.261
2.125ProGly: 2.125 ± 0.404
0.607ProHis: 0.607 ± 0.22
2.125ProIle: 2.125 ± 0.337
2.55ProLys: 2.55 ± 0.285
2.368ProLeu: 2.368 ± 0.434
0.911ProMet: 0.911 ± 0.229
1.518ProAsn: 1.518 ± 0.32
0.85ProPro: 0.85 ± 0.306
0.546ProGln: 0.546 ± 0.167
2.125ProArg: 2.125 ± 0.453
1.943ProSer: 1.943 ± 0.302
2.489ProThr: 2.489 ± 0.425
1.821ProVal: 1.821 ± 0.358
0.486ProTrp: 0.486 ± 0.196
1.639ProTyr: 1.639 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
2.853GlnAla: 2.853 ± 0.574
0.182GlnCys: 0.182 ± 0.15
1.396GlnAsp: 1.396 ± 0.367
2.064GlnGlu: 2.064 ± 0.338
0.911GlnPhe: 0.911 ± 0.256
1.578GlnGly: 1.578 ± 0.318
0.425GlnHis: 0.425 ± 0.156
2.125GlnIle: 2.125 ± 0.408
2.489GlnLys: 2.489 ± 0.312
2.853GlnLeu: 2.853 ± 0.427
1.032GlnMet: 1.032 ± 0.284
1.396GlnAsn: 1.396 ± 0.259
0.668GlnPro: 0.668 ± 0.208
0.911GlnGln: 0.911 ± 0.246
2.55GlnArg: 2.55 ± 0.443
1.275GlnSer: 1.275 ± 0.265
2.307GlnThr: 2.307 ± 0.467
1.7GlnVal: 1.7 ± 0.338
0.425GlnTrp: 0.425 ± 0.183
1.578GlnTyr: 1.578 ± 0.254
0.0GlnXaa: 0.0 ± 0.0
Arg
3.946ArgAla: 3.946 ± 0.459
0.729ArgCys: 0.729 ± 0.185
3.703ArgAsp: 3.703 ± 0.463
6.617ArgGlu: 6.617 ± 0.697
2.186ArgPhe: 2.186 ± 0.359
3.643ArgGly: 3.643 ± 0.458
1.275ArgHis: 1.275 ± 0.31
4.128ArgIle: 4.128 ± 0.415
6.374ArgLys: 6.374 ± 0.686
6.86ArgLeu: 6.86 ± 0.615
2.186ArgMet: 2.186 ± 0.378
3.218ArgAsn: 3.218 ± 0.476
1.7ArgPro: 1.7 ± 0.293
2.246ArgGln: 2.246 ± 0.416
4.128ArgArg: 4.128 ± 0.647
2.732ArgSer: 2.732 ± 0.445
2.671ArgThr: 2.671 ± 0.339
3.885ArgVal: 3.885 ± 0.555
1.093ArgTrp: 1.093 ± 0.265
2.793ArgTyr: 2.793 ± 0.372
0.0ArgXaa: 0.0 ± 0.0
Ser
4.432SerAla: 4.432 ± 0.464
0.85SerCys: 0.85 ± 0.245
3.035SerAsp: 3.035 ± 0.466
4.068SerGlu: 4.068 ± 0.599
2.307SerPhe: 2.307 ± 0.376
4.31SerGly: 4.31 ± 0.468
0.911SerHis: 0.911 ± 0.212
2.55SerIle: 2.55 ± 0.347
3.643SerLys: 3.643 ± 0.387
3.825SerLeu: 3.825 ± 0.5
1.457SerMet: 1.457 ± 0.286
1.518SerAsn: 1.518 ± 0.339
2.186SerPro: 2.186 ± 0.359
1.821SerGln: 1.821 ± 0.427
2.732SerArg: 2.732 ± 0.507
2.853SerSer: 2.853 ± 0.413
2.186SerThr: 2.186 ± 0.355
3.703SerVal: 3.703 ± 0.522
0.729SerTrp: 0.729 ± 0.22
2.003SerTyr: 2.003 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
5.221ThrAla: 5.221 ± 0.747
0.546ThrCys: 0.546 ± 0.193
3.582ThrAsp: 3.582 ± 0.555
4.492ThrGlu: 4.492 ± 0.494
2.368ThrPhe: 2.368 ± 0.376
4.735ThrGly: 4.735 ± 0.458
1.336ThrHis: 1.336 ± 0.239
2.793ThrIle: 2.793 ± 0.41
4.917ThrLys: 4.917 ± 0.698
4.675ThrLeu: 4.675 ± 0.466
0.971ThrMet: 0.971 ± 0.304
1.518ThrAsn: 1.518 ± 0.398
2.368ThrPro: 2.368 ± 0.352
2.064ThrGln: 2.064 ± 0.438
2.55ThrArg: 2.55 ± 0.335
2.793ThrSer: 2.793 ± 0.427
2.428ThrThr: 2.428 ± 0.429
4.068ThrVal: 4.068 ± 0.527
0.85ThrTrp: 0.85 ± 0.201
2.55ThrTyr: 2.55 ± 0.405
0.0ThrXaa: 0.0 ± 0.0
Val
5.767ValAla: 5.767 ± 0.577
0.85ValCys: 0.85 ± 0.237
4.614ValAsp: 4.614 ± 0.569
4.978ValGlu: 4.978 ± 0.504
2.489ValPhe: 2.489 ± 0.36
4.492ValGly: 4.492 ± 0.502
1.214ValHis: 1.214 ± 0.261
4.675ValIle: 4.675 ± 0.557
4.614ValLys: 4.614 ± 0.62
5.889ValLeu: 5.889 ± 0.588
1.639ValMet: 1.639 ± 0.301
2.307ValAsn: 2.307 ± 0.383
1.943ValPro: 1.943 ± 0.317
1.821ValGln: 1.821 ± 0.293
3.703ValArg: 3.703 ± 0.456
3.157ValSer: 3.157 ± 0.426
4.007ValThr: 4.007 ± 0.491
4.31ValVal: 4.31 ± 0.692
0.486ValTrp: 0.486 ± 0.168
2.186ValTyr: 2.186 ± 0.331
0.0ValXaa: 0.0 ± 0.0
Trp
1.093TrpAla: 1.093 ± 0.313
0.0TrpCys: 0.0 ± 0.0
1.032TrpAsp: 1.032 ± 0.317
1.275TrpGlu: 1.275 ± 0.309
0.486TrpPhe: 0.486 ± 0.163
0.607TrpGly: 0.607 ± 0.188
0.425TrpHis: 0.425 ± 0.178
0.668TrpIle: 0.668 ± 0.205
0.789TrpLys: 0.789 ± 0.205
1.457TrpLeu: 1.457 ± 0.341
0.304TrpMet: 0.304 ± 0.123
0.668TrpAsn: 0.668 ± 0.2
0.121TrpPro: 0.121 ± 0.087
1.032TrpGln: 1.032 ± 0.281
0.729TrpArg: 0.729 ± 0.193
0.486TrpSer: 0.486 ± 0.151
0.789TrpThr: 0.789 ± 0.237
1.275TrpVal: 1.275 ± 0.265
0.182TrpTrp: 0.182 ± 0.112
0.486TrpTyr: 0.486 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.278TyrAla: 3.278 ± 0.485
0.304TyrCys: 0.304 ± 0.164
3.218TyrAsp: 3.218 ± 0.475
3.157TyrGlu: 3.157 ± 0.407
1.7TyrPhe: 1.7 ± 0.32
3.885TyrGly: 3.885 ± 0.545
0.668TyrHis: 0.668 ± 0.2
2.732TyrIle: 2.732 ± 0.434
2.671TyrLys: 2.671 ± 0.452
3.582TyrLeu: 3.582 ± 0.432
0.668TyrMet: 0.668 ± 0.235
1.032TyrAsn: 1.032 ± 0.247
1.518TyrPro: 1.518 ± 0.331
0.971TyrGln: 0.971 ± 0.211
3.218TyrArg: 3.218 ± 0.495
1.761TyrSer: 1.761 ± 0.323
2.246TyrThr: 2.246 ± 0.327
2.975TyrVal: 2.975 ± 0.363
0.486TyrTrp: 0.486 ± 0.172
1.821TyrTyr: 1.821 ± 0.338
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16473 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski