Salmonella phage St162
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A291AXA4|A0A291AXA4_9CAUD Putative tail tape measure protein OS=Salmonella phage St162 OX=2024312 GN=St162_gp56 PE=4 SV=1
MM1 pKa = 6.5 VARR4 pKa = 11.84 YY5 pKa = 9.06 RR6 pKa = 11.84 FNYY9 pKa = 7.46 VQEE12 pKa = 4.15 MFEE15 pKa = 4.17 TDD17 pKa = 3.69 AGEE20 pKa = 4.25 QYY22 pKa = 11.04 CEE24 pKa = 4.14 TEE26 pKa = 3.49 RR27 pKa = 11.84 DD28 pKa = 3.19 EE29 pKa = 4.15 WVKK32 pKa = 11.35 YY33 pKa = 10.22 EE34 pKa = 5.22 DD35 pKa = 3.74 YY36 pKa = 11.67 AEE38 pKa = 4.29 LKK40 pKa = 10.48 EE41 pKa = 4.67 LLDD44 pKa = 3.9 ALAYY48 pKa = 10.45 SVLNYY53 pKa = 9.63 PLSPDD58 pKa = 3.48 TEE60 pKa = 4.14 EE61 pKa = 3.98 LAKK64 pKa = 10.75 GVLGDD69 pKa = 3.66
Molecular weight: 8.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.827
IPC2_protein 4.062
IPC_protein 3.948
Toseland 3.783
ProMoST 3.999
Dawson 3.91
Bjellqvist 4.113
Wikipedia 3.795
Rodwell 3.783
Grimsley 3.694
Solomon 3.884
Lehninger 3.846
Nozaki 4.024
DTASelect 4.151
Thurlkill 3.808
EMBOSS 3.808
Sillero 4.062
Patrickios 0.846
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.989
Protein with the highest isoelectric point:
>tr|A0A291AX61|A0A291AX61_9CAUD Uncharacterized protein OS=Salmonella phage St162 OX=2024312 GN=St162_gp24 PE=4 SV=1
MM1 pKa = 7.52 SLATDD6 pKa = 3.39 VLKK9 pKa = 10.75 RR10 pKa = 11.84 ANADD14 pKa = 3.31 LSPPSKK20 pKa = 10.13 STWADD25 pKa = 3.25 YY26 pKa = 10.34 MGLAIRR32 pKa = 11.84 KK33 pKa = 7.19 KK34 pKa = 10.47 AKK36 pKa = 9.65 RR37 pKa = 11.84 AAPKK41 pKa = 10.45 GPRR44 pKa = 11.84 LNRR47 pKa = 11.84 KK48 pKa = 8.69 KK49 pKa = 10.86 GKK51 pKa = 6.12 TVKK54 pKa = 10.27 HH55 pKa = 5.21 VAPKK59 pKa = 9.26 HH60 pKa = 4.44 EE61 pKa = 4.45 HH62 pKa = 5.01 VAGVAFNSRR71 pKa = 11.84 NNTWDD76 pKa = 3.12 AYY78 pKa = 10.49 FYY80 pKa = 11.23 NGVKK84 pKa = 9.82 TIRR87 pKa = 11.84 IGMFHH92 pKa = 5.74 TQARR96 pKa = 11.84 AIIARR101 pKa = 11.84 RR102 pKa = 11.84 IYY104 pKa = 9.65 MYY106 pKa = 9.49 WRR108 pKa = 11.84 KK109 pKa = 10.11 CGFDD113 pKa = 3.89 NIPNKK118 pKa = 9.56 PEE120 pKa = 3.47 RR121 pKa = 11.84 RR122 pKa = 11.84 LYY124 pKa = 10.2 TMRR127 pKa = 11.84 NCSDD131 pKa = 3.09 KK132 pKa = 11.38 SS133 pKa = 3.42
Molecular weight: 15.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.984
IPC_protein 10.687
Toseland 10.921
ProMoST 10.57
Dawson 10.994
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 11.359
Grimsley 11.038
Solomon 11.111
Lehninger 11.082
Nozaki 10.891
DTASelect 10.687
Thurlkill 10.906
EMBOSS 11.316
Sillero 10.921
Patrickios 11.082
IPC_peptide 11.111
IPC2_peptide 9.56
IPC2.peptide.svr19 8.356
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
62
0
62
12799
32
828
206.4
22.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.095 ± 0.543
1.102 ± 0.128
6.477 ± 0.237
6.274 ± 0.325
3.5 ± 0.178
7.782 ± 0.232
1.727 ± 0.182
5.055 ± 0.165
6.086 ± 0.337
7.813 ± 0.295
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.43 ± 0.128
4.563 ± 0.225
3.828 ± 0.207
3.992 ± 0.402
5.391 ± 0.23
5.68 ± 0.313
6.172 ± 0.403
7.016 ± 0.335
1.5 ± 0.169
3.516 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here