Phenylobacterium soli
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3905 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328AMB1|A0A328AMB1_9CAUL Peptidase_S8 domain-containing protein OS=Phenylobacterium soli OX=2170551 GN=DJ017_11645 PE=4 SV=1
MM1 pKa = 7.86 PSGPSGSLLVTPGGPITDD19 pKa = 3.4 STYY22 pKa = 8.35 TARR25 pKa = 11.84 SFRR28 pKa = 11.84 LTNTSTDD35 pKa = 3.44 GQQLNTVTIDD45 pKa = 2.91 ISGSVLGGMIYY56 pKa = 10.59 DD57 pKa = 3.86 PTGQAGDD64 pKa = 3.51 STARR68 pKa = 11.84 PFTIDD73 pKa = 3.09 SQTGTFSLVNATLSNGSDD91 pKa = 2.86 ATGYY95 pKa = 6.68 TTLTLQLSGFDD106 pKa = 3.89 PGEE109 pKa = 4.06 EE110 pKa = 4.15 LDD112 pKa = 4.53 FSIDD116 pKa = 3.17 VDD118 pKa = 4.01 PNSIRR123 pKa = 11.84 GTTSPGPGEE132 pKa = 4.39 SGSVAGVEE140 pKa = 4.23 LTGSQVTFGFASTTLSNEE158 pKa = 3.52 IFKK161 pKa = 10.93 QPTSPGGGVVSVEE174 pKa = 4.16 TGLPGAPTEE183 pKa = 4.44 TVTGVSGNTASVSVAQQTVNLTGPAGATVRR213 pKa = 11.84 VMVLEE218 pKa = 4.6 GAAYY222 pKa = 7.37 TQGVPSTSPQLGPYY236 pKa = 8.32 QANSAVAVATYY247 pKa = 10.83 DD248 pKa = 3.41 VTLDD252 pKa = 3.88 GQGHH256 pKa = 6.85 GSTPVTLTHH265 pKa = 6.39 TVTEE269 pKa = 4.45 GGLNIITSAVVGADD283 pKa = 3.19 GVVGPASDD291 pKa = 5.64 PITLAYY297 pKa = 9.98 NVSPPPPPPPPPPVAGVTIVQSGGSTAVTEE327 pKa = 4.71 GGATDD332 pKa = 3.87 TVTLALAKK340 pKa = 10.44 QPTANVTVTVAGDD353 pKa = 3.66 ADD355 pKa = 4.02 VTGAPQSFTFTPTNWSTPQTLTVTAVDD382 pKa = 3.55 DD383 pKa = 4.03 TFIEE387 pKa = 4.72 GTEE390 pKa = 4.03 TAHH393 pKa = 7.57 LGFTTASPDD402 pKa = 3.09 TSYY405 pKa = 11.95 NNLAVAPLNVTVTDD419 pKa = 4.06 NDD421 pKa = 3.4 QSSTPNFVQSQIAGLPDD438 pKa = 3.53 ANYY441 pKa = 9.88 TSLQFGPDD449 pKa = 2.79 GRR451 pKa = 11.84 LYY453 pKa = 10.53 VSKK456 pKa = 11.2 VDD458 pKa = 3.72 GTILALKK465 pKa = 9.35 LQQTTTGYY473 pKa = 9.99 QVAAGGIEE481 pKa = 4.26 TINAIKK487 pKa = 10.61 SITNYY492 pKa = 10.62 NDD494 pKa = 3.78 DD495 pKa = 3.89 GTLASAGYY503 pKa = 6.93 QTSRR507 pKa = 11.84 QVTGIFVTSGAAPAGGGAAPVVMYY531 pKa = 10.11 VSSSDD536 pKa = 3.34 PRR538 pKa = 11.84 IGAGVNGGDD547 pKa = 4.0 VNLDD551 pKa = 3.59 TNSGVISRR559 pKa = 11.84 LTQQTDD565 pKa = 3.88 GSWQRR570 pKa = 11.84 VDD572 pKa = 4.07 LVRR575 pKa = 11.84 GLPRR579 pKa = 11.84 SEE581 pKa = 4.58 EE582 pKa = 3.91 NHH584 pKa = 6.08 SVNGLQVTPDD594 pKa = 3.36 GTTMYY599 pKa = 10.62 LAIGGNTNMGAPSNNFAYY617 pKa = 9.1 TPEE620 pKa = 4.12 YY621 pKa = 10.28 AYY623 pKa = 10.86 SAAILKK629 pKa = 9.83 IDD631 pKa = 4.62 LVAIDD636 pKa = 4.72 AMASKK641 pKa = 9.72 TDD643 pKa = 3.53 QFGNTYY649 pKa = 10.35 KK650 pKa = 11.01 YY651 pKa = 10.97 NLPTLPASAGDD662 pKa = 4.78 PYY664 pKa = 10.92 PSDD667 pKa = 3.68 PFGGSDD673 pKa = 3.65 GFSQAKK679 pKa = 8.89 IVAGSPVQIFSPGYY693 pKa = 8.38 RR694 pKa = 11.84 NPYY697 pKa = 10.43 DD698 pKa = 3.93 LVLTQSGQIYY708 pKa = 8.42 TVDD711 pKa = 3.1 NGANAGWGGVPVNEE725 pKa = 4.23 GTPNVTNAPVEE736 pKa = 4.33 GGVDD740 pKa = 3.57 VPDD743 pKa = 3.45 QLFLVTQGFYY753 pKa = 10.75 GGHH756 pKa = 6.98 PNPIRR761 pKa = 11.84 ANPTGAGLYY770 pKa = 9.6 PNGSTTPTTTYY781 pKa = 9.12 PQGWPPVSTADD792 pKa = 3.91 PVEE795 pKa = 4.2 GDD797 pKa = 3.37 YY798 pKa = 11.05 RR799 pKa = 11.84 GPGQSGSLYY808 pKa = 10.81 SGFQASTNGITEE820 pKa = 4.17 YY821 pKa = 10.1 TASTFGNAMKK831 pKa = 10.77 GNLLIVSWDD840 pKa = 3.48 DD841 pKa = 3.08 QLYY844 pKa = 9.73 RR845 pKa = 11.84 VQLGANGTSVTSVTKK860 pKa = 9.3 LTNGSTVLGNGSPLDD875 pKa = 3.63 VTSPGDD881 pKa = 3.66 GQPFAGNIFVANYY894 pKa = 10.08 GGGITAFIPTASPPPPPPPPNVADD918 pKa = 3.68 SDD920 pKa = 3.95 GDD922 pKa = 4.31 GIVDD926 pKa = 3.52 TRR928 pKa = 11.84 DD929 pKa = 3.29 PFAVDD934 pKa = 3.82 ASNGTATTVSAGQTLTWEE952 pKa = 5.17 FSQNIAPPSPPGLFNMGFTGLMSNGVDD979 pKa = 4.19 GYY981 pKa = 9.39 TALYY985 pKa = 10.31 DD986 pKa = 3.68 PANIKK991 pKa = 10.35 PGGAASGFQVEE1002 pKa = 4.64 KK1003 pKa = 9.98 TGSGDD1008 pKa = 3.1 AHH1010 pKa = 7.18 LDD1012 pKa = 3.3 NHH1014 pKa = 6.27 QEE1016 pKa = 3.74 NGFQFGVHH1024 pKa = 6.17 LQPGVTGVVFQTTIDD1039 pKa = 3.62 NPFAGTPSTSASASKK1054 pKa = 10.59 SQGFFIGTGDD1064 pKa = 3.28 QDD1066 pKa = 3.64 NYY1068 pKa = 10.8 LKK1070 pKa = 10.93 VVATGAQGSGNIEE1083 pKa = 3.75 VDD1085 pKa = 3.55 TEE1087 pKa = 4.25 NAGVWASTAYY1097 pKa = 9.51 KK1098 pKa = 10.72 VNIISNSVGALDD1110 pKa = 5.11 TITLRR1115 pKa = 11.84 LNVDD1119 pKa = 3.41 VTTGLATPSWSYY1131 pKa = 9.6 TVNGVASTGSGAAVQLSGATLSALNGAYY1159 pKa = 8.95 TVDD1162 pKa = 3.41 GHH1164 pKa = 7.11 ASALAVGIISTSDD1177 pKa = 3.12 GTNDD1181 pKa = 3.47 PFTALWQNMSITASGSGGSPPPPPPPPPPPPPPPPPPPPPPSGAGVVLTATAGGSNLVGGSGDD1244 pKa = 3.54 DD1245 pKa = 3.64 TLVGGSGADD1254 pKa = 3.21 TMTGAGGADD1263 pKa = 3.09 HH1264 pKa = 7.12 FVFPHH1269 pKa = 6.56 LAWTSDD1275 pKa = 3.02 QVTDD1279 pKa = 4.07 FTDD1282 pKa = 3.91 GTDD1285 pKa = 4.17 LIDD1288 pKa = 3.66 VTGLLSDD1295 pKa = 4.07 AGYY1298 pKa = 8.93 TGSNPIADD1306 pKa = 4.42 GYY1308 pKa = 11.16 VKK1310 pKa = 10.84 LIANGSGGTWLYY1322 pKa = 10.78 FDD1324 pKa = 5.11 RR1325 pKa = 11.84 DD1326 pKa = 3.68 GTGTADD1332 pKa = 2.79 QWGTYY1337 pKa = 7.15 VANISGVTPSQLTSADD1353 pKa = 3.79 FVASGSPPPPPSPPPPPPPPPPPPPPPPPPPPPPPPPPPPPSGGGVVLTASAGGSNLVGGSGDD1416 pKa = 3.54 DD1417 pKa = 3.64 TLVGGSGADD1426 pKa = 3.21 TMTGAGGADD1435 pKa = 3.09 HH1436 pKa = 7.12 FVFPHH1441 pKa = 6.56 LAWTSDD1447 pKa = 3.02 QVTDD1451 pKa = 4.07 FTDD1454 pKa = 3.91 GTDD1457 pKa = 4.17 LIDD1460 pKa = 3.66 VTGLLSDD1467 pKa = 4.07 AGYY1470 pKa = 8.93 TGSNPIADD1478 pKa = 4.42 GYY1480 pKa = 11.16 VKK1482 pKa = 10.84 LIANGSGGTWLYY1494 pKa = 10.78 FDD1496 pKa = 5.11 RR1497 pKa = 11.84 DD1498 pKa = 3.68 GTGTADD1504 pKa = 2.79 QWGTYY1509 pKa = 7.15 VANISGVTPTQLTSADD1525 pKa = 3.97 FVASGSPPPPPSPPPPPPPPPPPPPPPPPPPPPPPPPSGGVVLTATAGGSNLVGGAGDD1583 pKa = 3.66 DD1584 pKa = 3.78 TLNAGSGPDD1593 pKa = 3.4 TLTGGGGADD1602 pKa = 3.08 HH1603 pKa = 6.43 MVWGDD1608 pKa = 3.77 VPWTAGHH1615 pKa = 5.6 VTDD1618 pKa = 5.07 FTLGADD1624 pKa = 3.93 KK1625 pKa = 11.05 LDD1627 pKa = 3.8 AKK1629 pKa = 10.92 AWLAKK1634 pKa = 10.14 VGYY1637 pKa = 10.42 AGTNPIADD1645 pKa = 3.75 QYY1647 pKa = 11.46 IKK1649 pKa = 10.62 LISDD1653 pKa = 4.1 GAGNTWVYY1661 pKa = 10.6 FDD1663 pKa = 5.13 RR1664 pKa = 11.84 DD1665 pKa = 3.58 GTGTADD1671 pKa = 2.63 KK1672 pKa = 10.06 WGTFVTTLDD1681 pKa = 3.42 HH1682 pKa = 6.11 VAPGSITLGDD1692 pKa = 4.32 WIIKK1696 pKa = 9.51
Molecular weight: 170.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.674
IPC2_protein 3.783
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.503
Solomon 3.859
Lehninger 3.821
Nozaki 3.961
DTASelect 4.291
Thurlkill 3.656
EMBOSS 3.834
Sillero 3.973
Patrickios 1.291
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A328AGA1|A0A328AGA1_9CAUL Uncharacterized protein OS=Phenylobacterium soli OX=2170551 GN=DJ017_00610 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 9.11 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.53 GFRR19 pKa = 11.84 LRR21 pKa = 11.84 MSTKK25 pKa = 10.17 NGQKK29 pKa = 9.5 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3905
0
3905
1208133
14
2196
309.4
33.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.292 ± 0.072
0.785 ± 0.012
5.572 ± 0.031
5.862 ± 0.044
3.495 ± 0.022
9.062 ± 0.042
1.928 ± 0.016
4.107 ± 0.027
3.176 ± 0.034
9.968 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.235 ± 0.021
2.225 ± 0.03
5.715 ± 0.033
3.181 ± 0.024
7.443 ± 0.043
4.795 ± 0.032
5.035 ± 0.047
7.491 ± 0.028
1.447 ± 0.016
2.187 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here