Phenylobacterium soli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Phenylobacterium

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3905 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A328AMB1|A0A328AMB1_9CAUL Peptidase_S8 domain-containing protein OS=Phenylobacterium soli OX=2170551 GN=DJ017_11645 PE=4 SV=1
MM1 pKa = 7.86PSGPSGSLLVTPGGPITDD19 pKa = 3.4STYY22 pKa = 8.35TARR25 pKa = 11.84SFRR28 pKa = 11.84LTNTSTDD35 pKa = 3.44GQQLNTVTIDD45 pKa = 2.91ISGSVLGGMIYY56 pKa = 10.59DD57 pKa = 3.86PTGQAGDD64 pKa = 3.51STARR68 pKa = 11.84PFTIDD73 pKa = 3.09SQTGTFSLVNATLSNGSDD91 pKa = 2.86ATGYY95 pKa = 6.68TTLTLQLSGFDD106 pKa = 3.89PGEE109 pKa = 4.06EE110 pKa = 4.15LDD112 pKa = 4.53FSIDD116 pKa = 3.17VDD118 pKa = 4.01PNSIRR123 pKa = 11.84GTTSPGPGEE132 pKa = 4.39SGSVAGVEE140 pKa = 4.23LTGSQVTFGFASTTLSNEE158 pKa = 3.52IFKK161 pKa = 10.93QPTSPGGGVVSVEE174 pKa = 4.16TGLPGAPTEE183 pKa = 4.44TVTGVSGNTASVSVAQQTVNLTGPAGATVRR213 pKa = 11.84VMVLEE218 pKa = 4.6GAAYY222 pKa = 7.37TQGVPSTSPQLGPYY236 pKa = 8.32QANSAVAVATYY247 pKa = 10.83DD248 pKa = 3.41VTLDD252 pKa = 3.88GQGHH256 pKa = 6.85GSTPVTLTHH265 pKa = 6.39TVTEE269 pKa = 4.45GGLNIITSAVVGADD283 pKa = 3.19GVVGPASDD291 pKa = 5.64PITLAYY297 pKa = 9.98NVSPPPPPPPPPPVAGVTIVQSGGSTAVTEE327 pKa = 4.71GGATDD332 pKa = 3.87TVTLALAKK340 pKa = 10.44QPTANVTVTVAGDD353 pKa = 3.66ADD355 pKa = 4.02VTGAPQSFTFTPTNWSTPQTLTVTAVDD382 pKa = 3.55DD383 pKa = 4.03TFIEE387 pKa = 4.72GTEE390 pKa = 4.03TAHH393 pKa = 7.57LGFTTASPDD402 pKa = 3.09TSYY405 pKa = 11.95NNLAVAPLNVTVTDD419 pKa = 4.06NDD421 pKa = 3.4QSSTPNFVQSQIAGLPDD438 pKa = 3.53ANYY441 pKa = 9.88TSLQFGPDD449 pKa = 2.79GRR451 pKa = 11.84LYY453 pKa = 10.53VSKK456 pKa = 11.2VDD458 pKa = 3.72GTILALKK465 pKa = 9.35LQQTTTGYY473 pKa = 9.99QVAAGGIEE481 pKa = 4.26TINAIKK487 pKa = 10.61SITNYY492 pKa = 10.62NDD494 pKa = 3.78DD495 pKa = 3.89GTLASAGYY503 pKa = 6.93QTSRR507 pKa = 11.84QVTGIFVTSGAAPAGGGAAPVVMYY531 pKa = 10.11VSSSDD536 pKa = 3.34PRR538 pKa = 11.84IGAGVNGGDD547 pKa = 4.0VNLDD551 pKa = 3.59TNSGVISRR559 pKa = 11.84LTQQTDD565 pKa = 3.88GSWQRR570 pKa = 11.84VDD572 pKa = 4.07LVRR575 pKa = 11.84GLPRR579 pKa = 11.84SEE581 pKa = 4.58EE582 pKa = 3.91NHH584 pKa = 6.08SVNGLQVTPDD594 pKa = 3.36GTTMYY599 pKa = 10.62LAIGGNTNMGAPSNNFAYY617 pKa = 9.1TPEE620 pKa = 4.12YY621 pKa = 10.28AYY623 pKa = 10.86SAAILKK629 pKa = 9.83IDD631 pKa = 4.62LVAIDD636 pKa = 4.72AMASKK641 pKa = 9.72TDD643 pKa = 3.53QFGNTYY649 pKa = 10.35KK650 pKa = 11.01YY651 pKa = 10.97NLPTLPASAGDD662 pKa = 4.78PYY664 pKa = 10.92PSDD667 pKa = 3.68PFGGSDD673 pKa = 3.65GFSQAKK679 pKa = 8.89IVAGSPVQIFSPGYY693 pKa = 8.38RR694 pKa = 11.84NPYY697 pKa = 10.43DD698 pKa = 3.93LVLTQSGQIYY708 pKa = 8.42TVDD711 pKa = 3.1NGANAGWGGVPVNEE725 pKa = 4.23GTPNVTNAPVEE736 pKa = 4.33GGVDD740 pKa = 3.57VPDD743 pKa = 3.45QLFLVTQGFYY753 pKa = 10.75GGHH756 pKa = 6.98PNPIRR761 pKa = 11.84ANPTGAGLYY770 pKa = 9.6PNGSTTPTTTYY781 pKa = 9.12PQGWPPVSTADD792 pKa = 3.91PVEE795 pKa = 4.2GDD797 pKa = 3.37YY798 pKa = 11.05RR799 pKa = 11.84GPGQSGSLYY808 pKa = 10.81SGFQASTNGITEE820 pKa = 4.17YY821 pKa = 10.1TASTFGNAMKK831 pKa = 10.77GNLLIVSWDD840 pKa = 3.48DD841 pKa = 3.08QLYY844 pKa = 9.73RR845 pKa = 11.84VQLGANGTSVTSVTKK860 pKa = 9.3LTNGSTVLGNGSPLDD875 pKa = 3.63VTSPGDD881 pKa = 3.66GQPFAGNIFVANYY894 pKa = 10.08GGGITAFIPTASPPPPPPPPNVADD918 pKa = 3.68SDD920 pKa = 3.95GDD922 pKa = 4.31GIVDD926 pKa = 3.52TRR928 pKa = 11.84DD929 pKa = 3.29PFAVDD934 pKa = 3.82ASNGTATTVSAGQTLTWEE952 pKa = 5.17FSQNIAPPSPPGLFNMGFTGLMSNGVDD979 pKa = 4.19GYY981 pKa = 9.39TALYY985 pKa = 10.31DD986 pKa = 3.68PANIKK991 pKa = 10.35PGGAASGFQVEE1002 pKa = 4.64KK1003 pKa = 9.98TGSGDD1008 pKa = 3.1AHH1010 pKa = 7.18LDD1012 pKa = 3.3NHH1014 pKa = 6.27QEE1016 pKa = 3.74NGFQFGVHH1024 pKa = 6.17LQPGVTGVVFQTTIDD1039 pKa = 3.62NPFAGTPSTSASASKK1054 pKa = 10.59SQGFFIGTGDD1064 pKa = 3.28QDD1066 pKa = 3.64NYY1068 pKa = 10.8LKK1070 pKa = 10.93VVATGAQGSGNIEE1083 pKa = 3.75VDD1085 pKa = 3.55TEE1087 pKa = 4.25NAGVWASTAYY1097 pKa = 9.51KK1098 pKa = 10.72VNIISNSVGALDD1110 pKa = 5.11TITLRR1115 pKa = 11.84LNVDD1119 pKa = 3.41VTTGLATPSWSYY1131 pKa = 9.6TVNGVASTGSGAAVQLSGATLSALNGAYY1159 pKa = 8.95TVDD1162 pKa = 3.41GHH1164 pKa = 7.11ASALAVGIISTSDD1177 pKa = 3.12GTNDD1181 pKa = 3.47PFTALWQNMSITASGSGGSPPPPPPPPPPPPPPPPPPPPPPSGAGVVLTATAGGSNLVGGSGDD1244 pKa = 3.54DD1245 pKa = 3.64TLVGGSGADD1254 pKa = 3.21TMTGAGGADD1263 pKa = 3.09HH1264 pKa = 7.12FVFPHH1269 pKa = 6.56LAWTSDD1275 pKa = 3.02QVTDD1279 pKa = 4.07FTDD1282 pKa = 3.91GTDD1285 pKa = 4.17LIDD1288 pKa = 3.66VTGLLSDD1295 pKa = 4.07AGYY1298 pKa = 8.93TGSNPIADD1306 pKa = 4.42GYY1308 pKa = 11.16VKK1310 pKa = 10.84LIANGSGGTWLYY1322 pKa = 10.78FDD1324 pKa = 5.11RR1325 pKa = 11.84DD1326 pKa = 3.68GTGTADD1332 pKa = 2.79QWGTYY1337 pKa = 7.15VANISGVTPSQLTSADD1353 pKa = 3.79FVASGSPPPPPSPPPPPPPPPPPPPPPPPPPPPPPPPPPPPSGGGVVLTASAGGSNLVGGSGDD1416 pKa = 3.54DD1417 pKa = 3.64TLVGGSGADD1426 pKa = 3.21TMTGAGGADD1435 pKa = 3.09HH1436 pKa = 7.12FVFPHH1441 pKa = 6.56LAWTSDD1447 pKa = 3.02QVTDD1451 pKa = 4.07FTDD1454 pKa = 3.91GTDD1457 pKa = 4.17LIDD1460 pKa = 3.66VTGLLSDD1467 pKa = 4.07AGYY1470 pKa = 8.93TGSNPIADD1478 pKa = 4.42GYY1480 pKa = 11.16VKK1482 pKa = 10.84LIANGSGGTWLYY1494 pKa = 10.78FDD1496 pKa = 5.11RR1497 pKa = 11.84DD1498 pKa = 3.68GTGTADD1504 pKa = 2.79QWGTYY1509 pKa = 7.15VANISGVTPTQLTSADD1525 pKa = 3.97FVASGSPPPPPSPPPPPPPPPPPPPPPPPPPPPPPPPSGGVVLTATAGGSNLVGGAGDD1583 pKa = 3.66DD1584 pKa = 3.78TLNAGSGPDD1593 pKa = 3.4TLTGGGGADD1602 pKa = 3.08HH1603 pKa = 6.43MVWGDD1608 pKa = 3.77VPWTAGHH1615 pKa = 5.6VTDD1618 pKa = 5.07FTLGADD1624 pKa = 3.93KK1625 pKa = 11.05LDD1627 pKa = 3.8AKK1629 pKa = 10.92AWLAKK1634 pKa = 10.14VGYY1637 pKa = 10.42AGTNPIADD1645 pKa = 3.75QYY1647 pKa = 11.46IKK1649 pKa = 10.62LISDD1653 pKa = 4.1GAGNTWVYY1661 pKa = 10.6FDD1663 pKa = 5.13RR1664 pKa = 11.84DD1665 pKa = 3.58GTGTADD1671 pKa = 2.63KK1672 pKa = 10.06WGTFVTTLDD1681 pKa = 3.42HH1682 pKa = 6.11VAPGSITLGDD1692 pKa = 4.32WIIKK1696 pKa = 9.51

Molecular weight:
170.59 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A328AGA1|A0A328AGA1_9CAUL Uncharacterized protein OS=Phenylobacterium soli OX=2170551 GN=DJ017_00610 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.11RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.53GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTKK25 pKa = 10.17NGQKK29 pKa = 9.5VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.34 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3905

0

3905

1208133

14

2196

309.4

33.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.292 ± 0.072

0.785 ± 0.012

5.572 ± 0.031

5.862 ± 0.044

3.495 ± 0.022

9.062 ± 0.042

1.928 ± 0.016

4.107 ± 0.027

3.176 ± 0.034

9.968 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.021

2.225 ± 0.03

5.715 ± 0.033

3.181 ± 0.024

7.443 ± 0.043

4.795 ± 0.032

5.035 ± 0.047

7.491 ± 0.028

1.447 ± 0.016

2.187 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski