Sphingomonas sp. YZ-8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3584 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A494TFV1|A0A494TFV1_9SPHN FAD-binding protein OS=Sphingomonas sp. YZ-8 OX=2319844 GN=D3Y57_09455 PE=3 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.71VVTDD7 pKa = 3.32ACIKK11 pKa = 9.7CKK13 pKa = 10.78YY14 pKa = 7.81MDD16 pKa = 4.28CVEE19 pKa = 4.32VCPVDD24 pKa = 4.15CFYY27 pKa = 11.35EE28 pKa = 4.72GDD30 pKa = 3.56NMLVINPSEE39 pKa = 4.67CIDD42 pKa = 4.07CGVCEE47 pKa = 4.44PEE49 pKa = 4.82CPAEE53 pKa = 5.13AILPDD58 pKa = 3.96TEE60 pKa = 4.37NGLEE64 pKa = 4.15KK65 pKa = 10.06WLEE68 pKa = 4.04LNTTFSAQWPNITRR82 pKa = 11.84SRR84 pKa = 11.84AAPADD89 pKa = 3.53ADD91 pKa = 3.64EE92 pKa = 4.85FKK94 pKa = 11.0SVEE97 pKa = 4.02GKK99 pKa = 10.03FEE101 pKa = 4.41KK102 pKa = 10.6FFSPEE107 pKa = 3.88PGQGDD112 pKa = 3.47

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A494TEW7|A0A494TEW7_9SPHN Methyltransferase domain-containing protein OS=Sphingomonas sp. YZ-8 OX=2319844 GN=D3Y57_08335 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.61GFRR19 pKa = 11.84HH20 pKa = 5.84RR21 pKa = 11.84MSTVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84IKK41 pKa = 10.86LSAA44 pKa = 3.84

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3584

0

3584

1076289

29

2348

300.3

32.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.742 ± 0.054

0.819 ± 0.014

5.859 ± 0.028

5.01 ± 0.038

3.604 ± 0.027

8.786 ± 0.038

2.016 ± 0.019

5.578 ± 0.026

3.221 ± 0.03

9.549 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.02

2.885 ± 0.026

5.006 ± 0.028

3.012 ± 0.022

6.881 ± 0.04

5.75 ± 0.027

5.862 ± 0.039

7.336 ± 0.034

1.352 ± 0.017

2.265 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski