Beggiatoa sp. PS
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6644 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7BSP7|A7BSP7_9GAMM Uncharacterized protein OS=Beggiatoa sp. PS OX=422289 GN=BGP_1754 PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 4.56 GEE4 pKa = 4.59 EE5 pKa = 4.46 VEE7 pKa = 5.13 SSTTLPLEE15 pKa = 4.74 GGLHH19 pKa = 5.93 SIKK22 pKa = 10.4 VEE24 pKa = 4.21 YY25 pKa = 10.34 VAGTSITVDD34 pKa = 2.66 WSEE37 pKa = 3.98 TSEE40 pKa = 3.87 IAGNNLSFDD49 pKa = 3.61 GMDD52 pKa = 4.8 DD53 pKa = 3.95 YY54 pKa = 12.21 VDD56 pKa = 3.89 LQTDD60 pKa = 3.94 VVEE63 pKa = 4.41 LAQADD68 pKa = 4.21 FSLEE72 pKa = 3.58 AWINTAYY79 pKa = 10.47 SGSGQGILVSNDD91 pKa = 3.13 ADD93 pKa = 4.02 SSWEE97 pKa = 3.82 LGEE100 pKa = 4.33 KK101 pKa = 10.15 AFHH104 pKa = 5.63 VTSSGTLQFVGWGNGYY120 pKa = 9.92 IKK122 pKa = 10.8 GSTAVNDD129 pKa = 4.81 GQWHH133 pKa = 5.53 HH134 pKa = 6.42 VAVVWDD140 pKa = 3.89 YY141 pKa = 10.14 TAGSGKK147 pKa = 10.02 IYY149 pKa = 10.59 LDD151 pKa = 3.94 GADD154 pKa = 4.1 DD155 pKa = 4.09 TDD157 pKa = 3.68 TGGTNYY163 pKa = 9.89 VASNANNAGDD173 pKa = 4.04 TLKK176 pKa = 10.45 IAYY179 pKa = 10.3 SNFNSSEE186 pKa = 3.72 APNYY190 pKa = 9.98 FNGGIDD196 pKa = 4.0 EE197 pKa = 4.2 VRR199 pKa = 11.84 VWNTARR205 pKa = 11.84 SQAEE209 pKa = 3.45 IQAYY213 pKa = 7.74 MKK215 pKa = 9.76 STLKK219 pKa = 9.88 GTEE222 pKa = 4.21 SNLVAYY228 pKa = 8.59 YY229 pKa = 10.43 QLDD232 pKa = 3.87 EE233 pKa = 4.64 SSGTTATDD241 pKa = 3.15 TTGNYY246 pKa = 10.39 DD247 pKa = 3.23 GTLNGDD253 pKa = 3.86 PTWGVSDD260 pKa = 5.28 APLTDD265 pKa = 4.26 LPAPGSALEE274 pKa = 4.42 LDD276 pKa = 3.73 GSGDD280 pKa = 4.54 YY281 pKa = 9.24 ITLPDD286 pKa = 4.46 DD287 pKa = 3.7 KK288 pKa = 10.86 TWFNGDD294 pKa = 3.43 LTIEE298 pKa = 4.17 SWVYY302 pKa = 8.04 PHH304 pKa = 7.3 SYY306 pKa = 10.98 ANWSRR311 pKa = 11.84 ILDD314 pKa = 3.68 IGQGSGDD321 pKa = 3.93 YY322 pKa = 10.8 NVVLALTNEE331 pKa = 4.44 TSGKK335 pKa = 9.02 PSFRR339 pKa = 11.84 VFDD342 pKa = 4.55 DD343 pKa = 3.42 NGNRR347 pKa = 11.84 NPLEE351 pKa = 4.43 SNTALTISTWNHH363 pKa = 4.82 IAVTLSGTTATMYY376 pKa = 10.81 INGAEE381 pKa = 4.24 SGSITNSYY389 pKa = 9.62 IPSDD393 pKa = 3.67 VTRR396 pKa = 11.84 DD397 pKa = 3.2 KK398 pKa = 11.45 AYY400 pKa = 9.7 IGHH403 pKa = 6.19 SNWSNDD409 pKa = 2.49 ADD411 pKa = 3.79 ANAVFDD417 pKa = 5.1 EE418 pKa = 3.93 MRR420 pKa = 11.84 IWDD423 pKa = 3.83 VARR426 pKa = 11.84 SQAEE430 pKa = 3.45 IQAYY434 pKa = 7.31 MNRR437 pKa = 11.84 TMDD440 pKa = 3.63 GNEE443 pKa = 4.15 SGLIAYY449 pKa = 9.03 YY450 pKa = 10.94 NFDD453 pKa = 3.63 EE454 pKa = 4.9 KK455 pKa = 10.91 AASSIAVLSDD465 pKa = 2.93 IAGNNLGTLNGNPTWVDD482 pKa = 2.39 SDD484 pKa = 4.27 APVSKK489 pKa = 10.23 MIAPGNAMSFDD500 pKa = 3.69 GVDD503 pKa = 3.79 DD504 pKa = 3.87 YY505 pKa = 12.18 VAFDD509 pKa = 3.88 DD510 pKa = 5.61 TIPLDD515 pKa = 3.53 DD516 pKa = 4.35 TYY518 pKa = 11.08 TIEE521 pKa = 4.93 GWFQTSNTGDD531 pKa = 3.17 ILSFTDD537 pKa = 3.52 SGNHH541 pKa = 4.88 YY542 pKa = 11.05 ALIEE546 pKa = 3.94 LAGNGIMRR554 pKa = 11.84 FLHH557 pKa = 6.08 RR558 pKa = 11.84 VPAGSANGTNIYY570 pKa = 10.53 SSAGYY575 pKa = 10.91 ADD577 pKa = 4.56 GSWHH581 pKa = 6.91 HH582 pKa = 6.23 FACVKK587 pKa = 10.76 DD588 pKa = 3.63 EE589 pKa = 4.14 TGMYY593 pKa = 9.69 IYY595 pKa = 10.09 MDD597 pKa = 4.33 GEE599 pKa = 4.5 QVASGSDD606 pKa = 3.39 SNAIGDD612 pKa = 4.03 SVEE615 pKa = 4.13 VVIGRR620 pKa = 11.84 LSPTNSSRR628 pKa = 11.84 YY629 pKa = 9.25 FEE631 pKa = 4.74 GSLDD635 pKa = 4.79 EE636 pKa = 3.91 IRR638 pKa = 11.84 IWNTARR644 pKa = 11.84 TQTEE648 pKa = 3.9 IQADD652 pKa = 4.07 MYY654 pKa = 10.64 RR655 pKa = 11.84 ALGSFEE661 pKa = 4.3 TGLVAYY667 pKa = 9.45 YY668 pKa = 10.72 KK669 pKa = 10.33 FDD671 pKa = 3.52 QDD673 pKa = 3.33 SGTEE677 pKa = 4.17 VVDD680 pKa = 3.15 ITSFDD685 pKa = 3.53 NDD687 pKa = 3.14 GTLNGGPSWVTSEE700 pKa = 4.37 VPMDD704 pKa = 3.59
Molecular weight: 75.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.834
IPC_protein 3.872
Toseland 3.643
ProMoST 4.012
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.541
Solomon 3.859
Lehninger 3.821
Nozaki 3.961
DTASelect 4.228
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 1.011
IPC_peptide 3.859
IPC2_peptide 3.973
IPC2.peptide.svr19 3.877
Protein with the highest isoelectric point:
>tr|A7BUP4|A7BUP4_9GAMM Uncharacterized protein OS=Beggiatoa sp. PS OX=422289 GN=BGP_5125 PE=4 SV=1
MM1 pKa = 7.51 TINRR5 pKa = 11.84 LMAAKK10 pKa = 9.97 PPMILKK16 pKa = 10.05 RR17 pKa = 11.84 QMALKK22 pKa = 10.05 PLMILKK28 pKa = 9.64 QRR30 pKa = 11.84 MVLKK34 pKa = 10.49 PPMILNRR41 pKa = 11.84 RR42 pKa = 11.84 MTINQLMALKK52 pKa = 9.29 PQMMHH57 pKa = 6.63 KK58 pKa = 10.13 LKK60 pKa = 10.61 HH61 pKa = 6.13 RR62 pKa = 11.84 SLLILL67 pKa = 3.99
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.994
IPC_protein 12.544
Toseland 12.72
ProMoST 13.203
Dawson 12.72
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.618
Grimsley 12.764
Solomon 13.203
Lehninger 13.115
Nozaki 12.72
DTASelect 12.705
Thurlkill 12.72
EMBOSS 13.217
Sillero 12.72
Patrickios 12.34
IPC_peptide 13.217
IPC2_peptide 12.193
IPC2.peptide.svr19 9.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6644
0
6644
1440897
17
3115
216.9
24.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.802 ± 0.033
1.049 ± 0.013
5.095 ± 0.027
6.617 ± 0.037
4.476 ± 0.029
5.911 ± 0.041
2.33 ± 0.016
7.528 ± 0.032
6.24 ± 0.041
10.618 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.017
4.931 ± 0.026
4.203 ± 0.025
5.056 ± 0.034
4.381 ± 0.028
6.031 ± 0.028
5.828 ± 0.035
5.868 ± 0.025
1.354 ± 0.015
3.335 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here