Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H1N1 subtype

Average proteome isoelectric point is 7.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P06821|M2_I34A1 Matrix protein 2 OS=Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) OX=211044 GN=M PE=1 SV=1
MM1 pKa = 8.06DD2 pKa = 6.23PNTVSSFQDD11 pKa = 2.76ILLRR15 pKa = 11.84MSKK18 pKa = 8.31MQLEE22 pKa = 4.55SSSGDD27 pKa = 3.52LNGMITQFEE36 pKa = 4.44SLKK39 pKa = 10.58LYY41 pKa = 10.39RR42 pKa = 11.84DD43 pKa = 3.79SLGEE47 pKa = 3.64AVMRR51 pKa = 11.84MGDD54 pKa = 3.57LHH56 pKa = 7.44SLQNRR61 pKa = 11.84NEE63 pKa = 3.78KK64 pKa = 9.44WRR66 pKa = 11.84EE67 pKa = 3.47QLGQKK72 pKa = 10.12FEE74 pKa = 4.56EE75 pKa = 4.15IRR77 pKa = 11.84WLIEE81 pKa = 3.85EE82 pKa = 4.31VRR84 pKa = 11.84HH85 pKa = 5.68KK86 pKa = 11.14LKK88 pKa = 9.34ITEE91 pKa = 4.12NSFEE95 pKa = 4.57QITFMQALHH104 pKa = 6.65LLLEE108 pKa = 4.52VEE110 pKa = 4.16QEE112 pKa = 3.38IRR114 pKa = 11.84TFSFQLII121 pKa = 3.9

Molecular weight: 14.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P0CK64|PAX_I34A1 Protein PA-X OS=Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) OX=211044 GN=PA PE=3 SV=1
MM1 pKa = 7.16GQEE4 pKa = 4.5QDD6 pKa = 4.02TPWILSTGHH15 pKa = 6.99ISTQKK20 pKa = 10.39RR21 pKa = 11.84QDD23 pKa = 3.37GQQTPKK29 pKa = 10.65LEE31 pKa = 4.38HH32 pKa = 6.57RR33 pKa = 11.84NSTRR37 pKa = 11.84LMGHH41 pKa = 5.94CQKK44 pKa = 9.71TMNQVVMPKK53 pKa = 10.26QIVYY57 pKa = 8.45WKK59 pKa = 9.9QWLSLRR65 pKa = 11.84NPILVFLKK73 pKa = 10.09TRR75 pKa = 11.84VLKK78 pKa = 10.5RR79 pKa = 11.84WRR81 pKa = 11.84LFSKK85 pKa = 10.95HH86 pKa = 5.1EE87 pKa = 3.95

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

5298

87

759

407.5

46.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.7 ± 0.442

1.755 ± 0.281

4.36 ± 0.224

7.512 ± 0.577

3.775 ± 0.243

6.474 ± 0.401

1.812 ± 0.163

6.644 ± 0.307

6.116 ± 0.312

8.097 ± 0.461

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.096 ± 0.364

5.247 ± 0.499

3.926 ± 0.16

3.945 ± 0.288

6.852 ± 0.562

7.456 ± 0.407

6.229 ± 0.335

5.681 ± 0.482

1.604 ± 0.218

2.718 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski