Desulfuribacillus stibiiarsenatis
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2918 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E5L866|A0A1E5L866_9BACL Rho_N domain-containing protein OS=Desulfuribacillus stibiiarsenatis OX=1390249 GN=BHU72_11990 PE=4 SV=1
MM1 pKa = 7.61 KK2 pKa = 9.74 ATVDD6 pKa = 3.19 QDD8 pKa = 3.98 LCISCGACIDD18 pKa = 3.5 ICSEE22 pKa = 3.95 VFDD25 pKa = 4.58 WNDD28 pKa = 3.17 DD29 pKa = 3.43 GKK31 pKa = 11.25 AHH33 pKa = 5.96 VKK35 pKa = 10.5 SNEE38 pKa = 3.72 ISSDD42 pKa = 3.51 LEE44 pKa = 4.13 DD45 pKa = 3.7 SVRR48 pKa = 11.84 EE49 pKa = 4.04 ASEE52 pKa = 4.15 SCPTDD57 pKa = 4.71 AIPISS62 pKa = 3.64
Molecular weight: 6.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.859
IPC_protein 3.795
Toseland 3.592
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.826
Protein with the highest isoelectric point:
>tr|A0A1E5L8J7|A0A1E5L8J7_9BACL Uncharacterized protein OS=Desulfuribacillus stibiiarsenatis OX=1390249 GN=BHU72_13405 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.49 KK14 pKa = 8.63 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 RR29 pKa = 11.84 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.69 GRR39 pKa = 11.84 KK40 pKa = 8.69 VLSAA44 pKa = 4.05
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.735
Grimsley 13.056
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.457
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2918
0
2918
900260
27
2477
308.5
34.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.811 ± 0.051
0.947 ± 0.017
5.216 ± 0.032
6.999 ± 0.053
4.2 ± 0.034
6.5 ± 0.042
2.149 ± 0.021
8.772 ± 0.046
6.63 ± 0.044
9.468 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.714 ± 0.023
4.717 ± 0.039
3.498 ± 0.032
4.153 ± 0.035
4.197 ± 0.029
5.934 ± 0.033
5.432 ± 0.042
7.097 ± 0.044
0.923 ± 0.016
3.644 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here