Escherichia phage D6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222YY85|A0A222YY85_9CAUD Uncharacterized protein OS=Escherichia phage D6 OX=2014434 GN=D6_91 PE=4 SV=1
MM1 pKa = 6.88NTIKK5 pKa = 10.42EE6 pKa = 4.38MPVEE10 pKa = 4.01RR11 pKa = 11.84DD12 pKa = 3.3EE13 pKa = 4.43YY14 pKa = 11.11GCWTHH19 pKa = 7.27PEE21 pKa = 4.09YY22 pKa = 11.03EE23 pKa = 4.32KK24 pKa = 11.01FCAGRR29 pKa = 11.84EE30 pKa = 4.13YY31 pKa = 10.52ISTEE35 pKa = 4.16EE36 pKa = 3.27FDD38 pKa = 4.76AWMKK42 pKa = 10.58EE43 pKa = 4.05NNLQWTIRR51 pKa = 11.84SMDD54 pKa = 3.81EE55 pKa = 4.95DD56 pKa = 5.67DD57 pKa = 5.23FDD59 pKa = 6.72LDD61 pKa = 4.33AAGPDD66 pKa = 3.18IAVWEE71 pKa = 4.31PEE73 pKa = 4.05RR74 pKa = 11.84PEE76 pKa = 4.2GEE78 pKa = 3.99GWFVGSIHH86 pKa = 6.53DD87 pKa = 4.13TEE89 pKa = 5.44DD90 pKa = 3.87GPVCIWLRR98 pKa = 11.84EE99 pKa = 4.0KK100 pKa = 10.79VAAA103 pKa = 4.6

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222YXX4|A0A222YXX4_9CAUD Uncharacterized protein OS=Escherichia phage D6 OX=2014434 GN=D6_52 PE=4 SV=1
MM1 pKa = 7.45SWRR4 pKa = 11.84GWGRR8 pKa = 11.84AEE10 pKa = 4.7IMILRR15 pKa = 11.84QCAGTMTVEE24 pKa = 4.33SIGRR28 pKa = 11.84LIGRR32 pKa = 11.84TGSAVRR38 pKa = 11.84TKK40 pKa = 10.72ARR42 pKa = 11.84QLRR45 pKa = 11.84ICMILKK51 pKa = 10.51GDD53 pKa = 3.8FHH55 pKa = 7.15PSAKK59 pKa = 9.74YY60 pKa = 9.38RR61 pKa = 11.84QGDD64 pKa = 3.56IEE66 pKa = 4.71LARR69 pKa = 11.84QLHH72 pKa = 5.8RR73 pKa = 11.84CGVPRR78 pKa = 11.84RR79 pKa = 11.84EE80 pKa = 3.92IAEE83 pKa = 4.2KK84 pKa = 11.08LEE86 pKa = 4.22MPLGMINQYY95 pKa = 10.52VYY97 pKa = 10.41FEE99 pKa = 4.07RR100 pKa = 11.84RR101 pKa = 11.84VYY103 pKa = 10.27EE104 pKa = 4.01VV105 pKa = 2.89

Molecular weight: 12.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

118

0

118

27189

27

2278

230.4

25.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.452 ± 0.263

1.022 ± 0.101

5.962 ± 0.227

6.955 ± 0.282

3.443 ± 0.144

6.591 ± 0.266

1.714 ± 0.115

6.234 ± 0.115

5.995 ± 0.229

8.22 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.659 ± 0.141

4.991 ± 0.231

3.888 ± 0.12

3.796 ± 0.136

5.925 ± 0.206

6.561 ± 0.233

5.69 ± 0.243

6.506 ± 0.183

1.273 ± 0.086

3.123 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski