Amino acid dipepetide frequency for Escherichia phage D6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.011AlaAla: 9.011 ± 0.628
0.993AlaCys: 0.993 ± 0.218
5.848AlaAsp: 5.848 ± 0.508
7.099AlaGlu: 7.099 ± 0.588
3.568AlaPhe: 3.568 ± 0.309
6.657AlaGly: 6.657 ± 0.502
0.993AlaHis: 0.993 ± 0.16
6.731AlaIle: 6.731 ± 0.434
4.818AlaLys: 4.818 ± 0.454
9.158AlaLeu: 9.158 ± 0.681
2.575AlaMet: 2.575 ± 0.347
4.303AlaAsn: 4.303 ± 0.39
3.053AlaPro: 3.053 ± 0.308
4.119AlaGln: 4.119 ± 0.357
5.517AlaArg: 5.517 ± 0.438
5.223AlaSer: 5.223 ± 0.448
5.223AlaThr: 5.223 ± 0.461
5.885AlaVal: 5.885 ± 0.425
1.067AlaTrp: 1.067 ± 0.221
2.685AlaTyr: 2.685 ± 0.269
0.0AlaXaa: 0.0 ± 0.0
Cys
0.846CysAla: 0.846 ± 0.19
0.037CysCys: 0.037 ± 0.039
0.662CysAsp: 0.662 ± 0.149
0.331CysGlu: 0.331 ± 0.107
0.257CysPhe: 0.257 ± 0.092
0.92CysGly: 0.92 ± 0.169
0.257CysHis: 0.257 ± 0.081
0.846CysIle: 0.846 ± 0.181
0.699CysLys: 0.699 ± 0.167
1.03CysLeu: 1.03 ± 0.221
0.147CysMet: 0.147 ± 0.066
0.478CysAsn: 0.478 ± 0.134
0.294CysPro: 0.294 ± 0.094
0.515CysGln: 0.515 ± 0.144
0.772CysArg: 0.772 ± 0.176
0.515CysSer: 0.515 ± 0.135
0.515CysThr: 0.515 ± 0.148
0.515CysVal: 0.515 ± 0.171
0.147CysTrp: 0.147 ± 0.066
0.441CysTyr: 0.441 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
6.253AspAla: 6.253 ± 0.446
0.478AspCys: 0.478 ± 0.157
4.23AspAsp: 4.23 ± 0.581
4.046AspGlu: 4.046 ± 0.502
2.575AspPhe: 2.575 ± 0.318
5.149AspGly: 5.149 ± 0.545
0.478AspHis: 0.478 ± 0.122
4.303AspIle: 4.303 ± 0.361
3.053AspLys: 3.053 ± 0.307
4.45AspLeu: 4.45 ± 0.393
1.913AspMet: 1.913 ± 0.25
2.428AspAsn: 2.428 ± 0.354
1.913AspPro: 1.913 ± 0.257
1.839AspGln: 1.839 ± 0.259
2.611AspArg: 2.611 ± 0.286
3.531AspSer: 3.531 ± 0.421
3.715AspThr: 3.715 ± 0.371
3.752AspVal: 3.752 ± 0.366
0.993AspTrp: 0.993 ± 0.163
1.913AspTyr: 1.913 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
6.51GluAla: 6.51 ± 0.523
0.662GluCys: 0.662 ± 0.182
3.605GluAsp: 3.605 ± 0.291
4.855GluGlu: 4.855 ± 0.533
2.464GluPhe: 2.464 ± 0.292
3.936GluGly: 3.936 ± 0.458
1.471GluHis: 1.471 ± 0.22
4.598GluIle: 4.598 ± 0.435
4.598GluLys: 4.598 ± 0.411
6.326GluLeu: 6.326 ± 0.437
2.097GluMet: 2.097 ± 0.331
3.163GluAsn: 3.163 ± 0.302
2.244GluPro: 2.244 ± 0.242
3.715GluGln: 3.715 ± 0.376
4.45GluArg: 4.45 ± 0.408
4.414GluSer: 4.414 ± 0.406
2.869GluThr: 2.869 ± 0.318
3.678GluVal: 3.678 ± 0.372
0.92GluTrp: 0.92 ± 0.175
2.979GluTyr: 2.979 ± 0.318
0.0GluXaa: 0.0 ± 0.0
Phe
2.611PheAla: 2.611 ± 0.261
0.662PheCys: 0.662 ± 0.167
1.986PheAsp: 1.986 ± 0.298
2.575PheGlu: 2.575 ± 0.253
0.993PhePhe: 0.993 ± 0.194
2.207PheGly: 2.207 ± 0.337
0.699PheHis: 0.699 ± 0.136
2.354PheIle: 2.354 ± 0.32
1.839PheLys: 1.839 ± 0.249
2.428PheLeu: 2.428 ± 0.295
0.92PheMet: 0.92 ± 0.186
2.097PheAsn: 2.097 ± 0.256
1.287PhePro: 1.287 ± 0.232
1.14PheGln: 1.14 ± 0.201
1.692PheArg: 1.692 ± 0.222
2.391PheSer: 2.391 ± 0.299
2.906PheThr: 2.906 ± 0.384
2.391PheVal: 2.391 ± 0.28
0.257PheTrp: 0.257 ± 0.103
0.993PheTyr: 0.993 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
5.444GlyAla: 5.444 ± 0.61
0.883GlyCys: 0.883 ± 0.167
4.156GlyAsp: 4.156 ± 0.437
4.634GlyGlu: 4.634 ± 0.386
2.391GlyPhe: 2.391 ± 0.301
4.708GlyGly: 4.708 ± 0.433
1.214GlyHis: 1.214 ± 0.159
3.862GlyIle: 3.862 ± 0.418
5.223GlyLys: 5.223 ± 0.353
4.708GlyLeu: 4.708 ± 0.386
1.839GlyMet: 1.839 ± 0.259
3.752GlyAsn: 3.752 ± 0.457
0.588GlyPro: 0.588 ± 0.152
1.876GlyGln: 1.876 ± 0.201
3.862GlyArg: 3.862 ± 0.448
4.267GlySer: 4.267 ± 0.428
3.494GlyThr: 3.494 ± 0.417
5.296GlyVal: 5.296 ± 0.385
1.14GlyTrp: 1.14 ± 0.196
2.575GlyTyr: 2.575 ± 0.289
0.0GlyXaa: 0.0 ± 0.0
His
1.434HisAla: 1.434 ± 0.205
0.368HisCys: 0.368 ± 0.127
0.92HisAsp: 0.92 ± 0.248
1.03HisGlu: 1.03 ± 0.263
0.736HisPhe: 0.736 ± 0.149
1.324HisGly: 1.324 ± 0.193
0.625HisHis: 0.625 ± 0.152
1.177HisIle: 1.177 ± 0.2
0.736HisLys: 0.736 ± 0.182
1.434HisLeu: 1.434 ± 0.271
0.294HisMet: 0.294 ± 0.104
0.625HisAsn: 0.625 ± 0.171
1.067HisPro: 1.067 ± 0.226
0.772HisGln: 0.772 ± 0.171
0.736HisArg: 0.736 ± 0.148
1.361HisSer: 1.361 ± 0.252
0.772HisThr: 0.772 ± 0.19
0.993HisVal: 0.993 ± 0.186
0.184HisTrp: 0.184 ± 0.088
0.552HisTyr: 0.552 ± 0.138
0.0HisXaa: 0.0 ± 0.0
Ile
5.959IleAla: 5.959 ± 0.477
0.625IleCys: 0.625 ± 0.144
4.45IleAsp: 4.45 ± 0.412
4.634IleGlu: 4.634 ± 0.388
2.023IlePhe: 2.023 ± 0.239
3.788IleGly: 3.788 ± 0.324
1.067IleHis: 1.067 ± 0.202
3.237IleIle: 3.237 ± 0.341
4.046IleLys: 4.046 ± 0.453
4.083IleLeu: 4.083 ± 0.409
1.618IleMet: 1.618 ± 0.234
4.23IleAsn: 4.23 ± 0.434
3.126IlePro: 3.126 ± 0.319
1.729IleGln: 1.729 ± 0.233
3.678IleArg: 3.678 ± 0.376
4.818IleSer: 4.818 ± 0.417
4.009IleThr: 4.009 ± 0.422
3.237IleVal: 3.237 ± 0.352
0.588IleTrp: 0.588 ± 0.15
1.398IleTyr: 1.398 ± 0.287
0.0IleXaa: 0.0 ± 0.0
Lys
6.657LysAla: 6.657 ± 0.513
0.515LysCys: 0.515 ± 0.164
3.274LysAsp: 3.274 ± 0.339
3.972LysGlu: 3.972 ± 0.417
1.287LysPhe: 1.287 ± 0.202
3.641LysGly: 3.641 ± 0.412
1.251LysHis: 1.251 ± 0.188
3.494LysIle: 3.494 ± 0.373
3.899LysLys: 3.899 ± 0.445
5.002LysLeu: 5.002 ± 0.455
1.692LysMet: 1.692 ± 0.265
3.2LysAsn: 3.2 ± 0.321
2.391LysPro: 2.391 ± 0.317
2.722LysGln: 2.722 ± 0.324
3.972LysArg: 3.972 ± 0.395
3.899LysSer: 3.899 ± 0.346
3.237LysThr: 3.237 ± 0.329
3.347LysVal: 3.347 ± 0.378
0.699LysTrp: 0.699 ± 0.146
1.765LysTyr: 1.765 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
7.65LeuAla: 7.65 ± 0.538
1.067LeuCys: 1.067 ± 0.188
4.708LeuAsp: 4.708 ± 0.467
5.848LeuGlu: 5.848 ± 0.588
2.428LeuPhe: 2.428 ± 0.346
3.862LeuGly: 3.862 ± 0.352
1.655LeuHis: 1.655 ± 0.222
4.745LeuIle: 4.745 ± 0.423
5.186LeuLys: 5.186 ± 0.453
6.4LeuLeu: 6.4 ± 0.586
2.06LeuMet: 2.06 ± 0.354
4.414LeuAsn: 4.414 ± 0.395
3.531LeuPro: 3.531 ± 0.343
3.053LeuGln: 3.053 ± 0.318
5.113LeuArg: 5.113 ± 0.45
6.216LeuSer: 6.216 ± 0.425
5.407LeuThr: 5.407 ± 0.369
6.106LeuVal: 6.106 ± 0.41
0.552LeuTrp: 0.552 ± 0.138
2.207LeuTyr: 2.207 ± 0.364
0.0LeuXaa: 0.0 ± 0.0
Met
3.09MetAla: 3.09 ± 0.331
0.11MetCys: 0.11 ± 0.06
1.324MetAsp: 1.324 ± 0.185
1.434MetGlu: 1.434 ± 0.224
1.103MetPhe: 1.103 ± 0.203
1.251MetGly: 1.251 ± 0.186
0.331MetHis: 0.331 ± 0.103
1.655MetIle: 1.655 ± 0.27
1.582MetLys: 1.582 ± 0.238
2.28MetLeu: 2.28 ± 0.331
0.772MetMet: 0.772 ± 0.178
1.361MetAsn: 1.361 ± 0.207
1.251MetPro: 1.251 ± 0.193
1.03MetGln: 1.03 ± 0.197
2.133MetArg: 2.133 ± 0.321
2.06MetSer: 2.06 ± 0.272
1.913MetThr: 1.913 ± 0.244
1.214MetVal: 1.214 ± 0.208
0.184MetTrp: 0.184 ± 0.084
0.515MetTyr: 0.515 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
4.855AsnAla: 4.855 ± 0.456
0.552AsnCys: 0.552 ± 0.154
2.611AsnAsp: 2.611 ± 0.283
2.979AsnGlu: 2.979 ± 0.313
1.434AsnPhe: 1.434 ± 0.228
5.26AsnGly: 5.26 ± 0.663
0.625AsnHis: 0.625 ± 0.134
3.899AsnIle: 3.899 ± 0.365
3.347AsnLys: 3.347 ± 0.369
3.2AsnLeu: 3.2 ± 0.315
0.92AsnMet: 0.92 ± 0.152
3.09AsnAsn: 3.09 ± 0.423
2.759AsnPro: 2.759 ± 0.309
1.692AsnGln: 1.692 ± 0.273
2.869AsnArg: 2.869 ± 0.331
1.986AsnSer: 1.986 ± 0.27
2.575AsnThr: 2.575 ± 0.383
2.722AsnVal: 2.722 ± 0.298
0.993AsnTrp: 0.993 ± 0.212
1.545AsnTyr: 1.545 ± 0.197
0.0AsnXaa: 0.0 ± 0.0
Pro
3.605ProAla: 3.605 ± 0.328
0.441ProCys: 0.441 ± 0.138
3.126ProAsp: 3.126 ± 0.36
4.34ProGlu: 4.34 ± 0.459
1.398ProPhe: 1.398 ± 0.233
2.133ProGly: 2.133 ± 0.281
0.736ProHis: 0.736 ± 0.166
1.692ProIle: 1.692 ± 0.248
1.876ProLys: 1.876 ± 0.26
3.163ProLeu: 3.163 ± 0.386
0.736ProMet: 0.736 ± 0.149
1.177ProAsn: 1.177 ± 0.209
1.434ProPro: 1.434 ± 0.203
1.545ProGln: 1.545 ± 0.23
1.618ProArg: 1.618 ± 0.271
2.538ProSer: 2.538 ± 0.246
2.17ProThr: 2.17 ± 0.272
3.31ProVal: 3.31 ± 0.322
0.552ProTrp: 0.552 ± 0.129
1.287ProTyr: 1.287 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
3.972GlnAla: 3.972 ± 0.351
0.405GlnCys: 0.405 ± 0.116
1.618GlnAsp: 1.618 ± 0.254
2.17GlnGlu: 2.17 ± 0.293
1.618GlnPhe: 1.618 ± 0.209
2.244GlnGly: 2.244 ± 0.233
0.699GlnHis: 0.699 ± 0.162
1.913GlnIle: 1.913 ± 0.279
2.942GlnLys: 2.942 ± 0.372
3.421GlnLeu: 3.421 ± 0.312
1.067GlnMet: 1.067 ± 0.188
1.729GlnAsn: 1.729 ± 0.23
1.655GlnPro: 1.655 ± 0.267
1.913GlnGln: 1.913 ± 0.308
2.133GlnArg: 2.133 ± 0.369
2.17GlnSer: 2.17 ± 0.313
1.949GlnThr: 1.949 ± 0.371
2.244GlnVal: 2.244 ± 0.252
0.294GlnTrp: 0.294 ± 0.096
1.802GlnTyr: 1.802 ± 0.223
0.0GlnXaa: 0.0 ± 0.0
Arg
5.26ArgAla: 5.26 ± 0.488
0.221ArgCys: 0.221 ± 0.098
3.788ArgAsp: 3.788 ± 0.353
4.855ArgGlu: 4.855 ± 0.468
2.317ArgPhe: 2.317 ± 0.24
2.942ArgGly: 2.942 ± 0.22
1.067ArgHis: 1.067 ± 0.187
3.862ArgIle: 3.862 ± 0.34
3.936ArgLys: 3.936 ± 0.371
5.186ArgLeu: 5.186 ± 0.539
1.287ArgMet: 1.287 ± 0.216
3.2ArgAsn: 3.2 ± 0.336
1.913ArgPro: 1.913 ± 0.235
2.317ArgGln: 2.317 ± 0.29
4.046ArgArg: 4.046 ± 0.375
3.421ArgSer: 3.421 ± 0.354
2.942ArgThr: 2.942 ± 0.38
3.862ArgVal: 3.862 ± 0.383
1.067ArgTrp: 1.067 ± 0.228
1.765ArgTyr: 1.765 ± 0.259
0.0ArgXaa: 0.0 ± 0.0
Ser
6.437SerAla: 6.437 ± 0.443
0.588SerCys: 0.588 ± 0.145
4.009SerAsp: 4.009 ± 0.402
3.972SerGlu: 3.972 ± 0.357
2.06SerPhe: 2.06 ± 0.25
4.929SerGly: 4.929 ± 0.482
1.287SerHis: 1.287 ± 0.218
3.862SerIle: 3.862 ± 0.354
3.457SerLys: 3.457 ± 0.365
6.179SerLeu: 6.179 ± 0.53
1.765SerMet: 1.765 ± 0.288
2.354SerAsn: 2.354 ± 0.279
3.053SerPro: 3.053 ± 0.335
2.538SerGln: 2.538 ± 0.327
4.009SerArg: 4.009 ± 0.333
3.605SerSer: 3.605 ± 0.417
3.457SerThr: 3.457 ± 0.369
5.223SerVal: 5.223 ± 0.557
1.067SerTrp: 1.067 ± 0.161
1.765SerTyr: 1.765 ± 0.29
0.0SerXaa: 0.0 ± 0.0
Thr
5.922ThrAla: 5.922 ± 0.504
0.257ThrCys: 0.257 ± 0.083
3.237ThrAsp: 3.237 ± 0.368
3.384ThrGlu: 3.384 ± 0.394
1.765ThrPhe: 1.765 ± 0.299
4.634ThrGly: 4.634 ± 0.578
0.92ThrHis: 0.92 ± 0.176
3.347ThrIle: 3.347 ± 0.35
3.237ThrLys: 3.237 ± 0.387
4.965ThrLeu: 4.965 ± 0.436
1.177ThrMet: 1.177 ± 0.244
2.464ThrAsn: 2.464 ± 0.326
3.163ThrPro: 3.163 ± 0.322
1.949ThrGln: 1.949 ± 0.247
2.832ThrArg: 2.832 ± 0.339
4.119ThrSer: 4.119 ± 0.435
3.568ThrThr: 3.568 ± 0.359
3.678ThrVal: 3.678 ± 0.366
0.588ThrTrp: 0.588 ± 0.163
1.692ThrTyr: 1.692 ± 0.256
0.0ThrXaa: 0.0 ± 0.0
Val
5.922ValAla: 5.922 ± 0.44
0.662ValCys: 0.662 ± 0.173
3.715ValAsp: 3.715 ± 0.345
4.377ValGlu: 4.377 ± 0.38
2.133ValPhe: 2.133 ± 0.236
3.715ValGly: 3.715 ± 0.473
0.993ValHis: 0.993 ± 0.177
4.34ValIle: 4.34 ± 0.377
3.752ValLys: 3.752 ± 0.376
4.892ValLeu: 4.892 ± 0.485
2.097ValMet: 2.097 ± 0.283
3.788ValAsn: 3.788 ± 0.353
2.317ValPro: 2.317 ± 0.298
1.655ValGln: 1.655 ± 0.233
3.641ValArg: 3.641 ± 0.337
6.179ValSer: 6.179 ± 0.525
3.752ValThr: 3.752 ± 0.392
4.377ValVal: 4.377 ± 0.458
0.662ValTrp: 0.662 ± 0.174
2.097ValTyr: 2.097 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
0.956TrpAla: 0.956 ± 0.191
0.184TrpCys: 0.184 ± 0.078
0.588TrpAsp: 0.588 ± 0.169
0.846TrpGlu: 0.846 ± 0.157
0.736TrpPhe: 0.736 ± 0.161
0.478TrpGly: 0.478 ± 0.136
0.147TrpHis: 0.147 ± 0.057
0.736TrpIle: 0.736 ± 0.162
0.441TrpLys: 0.441 ± 0.132
1.361TrpLeu: 1.361 ± 0.167
0.846TrpMet: 0.846 ± 0.158
0.515TrpAsn: 0.515 ± 0.147
0.441TrpPro: 0.441 ± 0.131
0.478TrpGln: 0.478 ± 0.124
0.883TrpArg: 0.883 ± 0.161
0.956TrpSer: 0.956 ± 0.254
0.662TrpThr: 0.662 ± 0.168
0.846TrpVal: 0.846 ± 0.166
0.221TrpTrp: 0.221 ± 0.074
0.405TrpTyr: 0.405 ± 0.13
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.133TyrAla: 2.133 ± 0.227
0.515TyrCys: 0.515 ± 0.105
1.765TyrAsp: 1.765 ± 0.26
2.17TyrGlu: 2.17 ± 0.321
1.14TyrPhe: 1.14 ± 0.206
2.133TyrGly: 2.133 ± 0.264
0.625TyrHis: 0.625 ± 0.139
1.692TyrIle: 1.692 ± 0.227
1.287TyrLys: 1.287 ± 0.208
2.538TyrLeu: 2.538 ± 0.332
0.736TyrMet: 0.736 ± 0.162
1.545TyrAsn: 1.545 ± 0.222
1.398TyrPro: 1.398 ± 0.219
1.361TyrGln: 1.361 ± 0.214
2.685TyrArg: 2.685 ± 0.311
1.949TyrSer: 1.949 ± 0.282
1.765TyrThr: 1.765 ± 0.233
2.391TyrVal: 2.391 ± 0.318
0.552TyrTrp: 0.552 ± 0.126
0.846TyrTyr: 0.846 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 118 proteins (27189 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski