Salmonella phage Munch

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 538 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3T0IFE7|A0A3T0IFE7_9CAUD O-spannin OS=Salmonella phage Munch OX=2500169 GN=CPT_Munch_183 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 4.8EE3 pKa = 4.48YY4 pKa = 11.27EE5 pKa = 4.7DD6 pKa = 3.79MRR8 pKa = 11.84SMMDD12 pKa = 3.39YY13 pKa = 11.05VDD15 pKa = 3.88STEE18 pKa = 4.01QSDD21 pKa = 5.43DD22 pKa = 3.15IDD24 pKa = 5.88AEE26 pKa = 4.21LLTFVDD32 pKa = 4.01NSIFMEE38 pKa = 4.44SQAHH42 pKa = 6.02HH43 pKa = 6.13WHH45 pKa = 6.35LQCNYY50 pKa = 9.08YY51 pKa = 9.76SKK53 pKa = 10.99HH54 pKa = 5.37MEE56 pKa = 3.88LDD58 pKa = 3.14EE59 pKa = 5.06FYY61 pKa = 11.0EE62 pKa = 4.32DD63 pKa = 4.67LPEE66 pKa = 4.53LVDD69 pKa = 3.7TFVEE73 pKa = 4.53GLMNTRR79 pKa = 11.84GPLPIGMDD87 pKa = 3.29AQYY90 pKa = 10.74TFVPLEE96 pKa = 4.06DD97 pKa = 5.42AISTLEE103 pKa = 4.1VYY105 pKa = 10.27VEE107 pKa = 3.98QARR110 pKa = 11.84QIHH113 pKa = 6.12RR114 pKa = 11.84LLDD117 pKa = 3.41EE118 pKa = 4.9RR119 pKa = 11.84EE120 pKa = 4.29DD121 pKa = 3.87FGSVNSLEE129 pKa = 5.0DD130 pKa = 3.31IISFVEE136 pKa = 3.79ATLYY140 pKa = 10.26KK141 pKa = 10.41LKK143 pKa = 10.61HH144 pKa = 5.39LQQ146 pKa = 3.23

Molecular weight: 17.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3T0IFG0|A0A3T0IFG0_9CAUD Uncharacterized protein OS=Salmonella phage Munch OX=2500169 GN=CPT_Munch_212 PE=4 SV=1
MM1 pKa = 7.71ARR3 pKa = 11.84GYY5 pKa = 8.73WSKK8 pKa = 10.86RR9 pKa = 11.84GYY11 pKa = 10.13SLNGLNPNAYY21 pKa = 8.85TSKK24 pKa = 9.87TDD26 pKa = 3.88KK27 pKa = 11.06DD28 pKa = 3.79GNLINLPSASLMKK41 pKa = 10.31FWKK44 pKa = 10.0IRR46 pKa = 11.84DD47 pKa = 3.62MPFGSEE53 pKa = 3.33RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84AVIEE60 pKa = 3.9LLKK63 pKa = 9.97TGYY66 pKa = 10.27LIPVNGIGQIQLKK79 pKa = 9.99CDD81 pKa = 3.68PDD83 pKa = 3.58LRR85 pKa = 11.84KK86 pKa = 9.67LLKK89 pKa = 10.35DD90 pKa = 4.2EE91 pKa = 5.02KK92 pKa = 9.97IQMYY96 pKa = 9.43NEE98 pKa = 3.9RR99 pKa = 11.84NRR101 pKa = 11.84TFKK104 pKa = 10.38RR105 pKa = 11.84TIIRR109 pKa = 11.84YY110 pKa = 8.54NDD112 pKa = 2.96GSEE115 pKa = 3.62RR116 pKa = 11.84RR117 pKa = 11.84KK118 pKa = 10.09RR119 pKa = 11.84KK120 pKa = 9.73RR121 pKa = 11.84SS122 pKa = 3.31

Molecular weight: 14.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

538

0

538

108363

42

3342

201.4

22.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.278 ± 0.127

1.195 ± 0.055

6.676 ± 0.084

7.205 ± 0.162

4.525 ± 0.083

5.986 ± 0.138

1.987 ± 0.069

7.121 ± 0.111

6.906 ± 0.14

7.693 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.929 ± 0.07

6.104 ± 0.114

3.211 ± 0.071

3.476 ± 0.086

4.055 ± 0.074

6.729 ± 0.109

6.256 ± 0.179

6.568 ± 0.097

1.15 ± 0.041

4.952 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski