Amaricoccus sp. HB172011
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4522 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A501WPD3|A0A501WPD3_9RHOB Alpha/beta hydrolase OS=Amaricoccus sp. HB172011 OX=2589815 GN=FJM51_12510 PE=4 SV=1
MM1 pKa = 7.6 IYY3 pKa = 8.85 KK4 pKa = 8.34 TARR7 pKa = 11.84 GASLSTSGAPTSWIGAEE24 pKa = 3.4 IGAYY28 pKa = 10.46 LSGTSGNDD36 pKa = 2.94 EE37 pKa = 4.79 LYY39 pKa = 11.13 GNDD42 pKa = 3.42 SATLAGGLGDD52 pKa = 3.99 DD53 pKa = 4.76 TYY55 pKa = 10.88 MVWVPSTHH63 pKa = 5.72 VLEE66 pKa = 4.6 AANAGIDD73 pKa = 4.37 TLDD76 pKa = 3.08 SWIWGTTVLPEE87 pKa = 3.9 NFEE90 pKa = 4.44 NLFLNGPGSTGGTGNASANIIAAGEE115 pKa = 4.11 VGATLDD121 pKa = 3.85 GLGGDD126 pKa = 4.37 DD127 pKa = 4.14 VLIGGAAADD136 pKa = 3.55 IFRR139 pKa = 11.84 IEE141 pKa = 4.52 AGNGSDD147 pKa = 4.53 AIVGFTPGSDD157 pKa = 3.37 VIEE160 pKa = 4.98 LIDD163 pKa = 3.55 YY164 pKa = 8.39 GFSNFAQVKK173 pKa = 10.17 AIATKK178 pKa = 9.52 TGKK181 pKa = 10.15 DD182 pKa = 3.48 LTLHH186 pKa = 6.92 LAQDD190 pKa = 4.45 EE191 pKa = 4.46 ILVLRR196 pKa = 11.84 DD197 pKa = 3.4 TKK199 pKa = 10.74 LAQLDD204 pKa = 4.55 GYY206 pKa = 11.07 DD207 pKa = 4.11 FGFPPPAPAPGAGQGSLSGPGAVYY231 pKa = 9.88 TDD233 pKa = 3.52 HH234 pKa = 6.59 GWYY237 pKa = 10.09 VLNNAWNAGGLTYY250 pKa = 9.56 GTDD253 pKa = 3.32 YY254 pKa = 11.16 RR255 pKa = 11.84 LDD257 pKa = 4.51 AIYY260 pKa = 10.82 DD261 pKa = 4.0 PDD263 pKa = 4.67 DD264 pKa = 3.71 LTTGVTFSWSFPRR277 pKa = 11.84 TTQAYY282 pKa = 8.37 PPILAYY288 pKa = 9.96 PEE290 pKa = 5.0 VIFGPAPMSGGHH302 pKa = 6.16 KK303 pKa = 9.62 VTDD306 pKa = 3.5 TADD309 pKa = 3.87 FFPLQVGEE317 pKa = 4.26 IQDD320 pKa = 3.43 LTAAYY325 pKa = 9.68 DD326 pKa = 3.34 VRR328 pKa = 11.84 YY329 pKa = 10.48 DD330 pKa = 4.97 GNTDD334 pKa = 2.98 WFNVAFDD341 pKa = 3.13 IWLTDD346 pKa = 3.44 VPNGGPSSVTNEE358 pKa = 3.53 VMVWVHH364 pKa = 5.94 QGAVKK369 pKa = 10.49 PFGDD373 pKa = 3.86 QVGTYY378 pKa = 10.43 SDD380 pKa = 3.4 GTIAAKK386 pKa = 10.01 IHH388 pKa = 6.59 SDD390 pKa = 4.24 DD391 pKa = 4.29 SSDD394 pKa = 2.96 WTYY397 pKa = 10.23 TAVVLDD403 pKa = 3.75 SDD405 pKa = 4.56 HH406 pKa = 6.93 PVGTISITGILSEE419 pKa = 4.77 LEE421 pKa = 3.93 SLGIVSPDD429 pKa = 3.23 EE430 pKa = 4.3 YY431 pKa = 10.47 IGSVEE436 pKa = 4.14 LGSEE440 pKa = 4.05 IVAGAGRR447 pKa = 11.84 LTINEE452 pKa = 4.18 LTLTARR458 pKa = 11.84 LDD460 pKa = 3.6 DD461 pKa = 3.89 RR462 pKa = 11.84 TLEE465 pKa = 4.08 SSGAEE470 pKa = 4.0 TTVTLLDD477 pKa = 3.82 GAGTQTHH484 pKa = 6.48 AADD487 pKa = 3.94 TGWFF491 pKa = 3.58
Molecular weight: 51.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.948
Patrickios 1.901
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A0A501WXA2|A0A501WXA2_9RHOB Uncharacterized protein OS=Amaricoccus sp. HB172011 OX=2589815 GN=FJM51_11985 PE=4 SV=1
MM1 pKa = 7.77 ALTSSCVSRR10 pKa = 11.84 ARR12 pKa = 11.84 RR13 pKa = 11.84 PARR16 pKa = 11.84 PRR18 pKa = 11.84 SSPAGRR24 pKa = 11.84 RR25 pKa = 11.84 GRR27 pKa = 11.84 RR28 pKa = 11.84 GARR31 pKa = 11.84 PSGAARR37 pKa = 11.84 SPSARR42 pKa = 11.84 CRR44 pKa = 11.84 ARR46 pKa = 11.84 PGRR49 pKa = 11.84 APSPRR54 pKa = 11.84 RR55 pKa = 11.84 RR56 pKa = 11.84 PPPGAGPAPRR66 pKa = 11.84 RR67 pKa = 11.84 GPARR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 RR74 pKa = 11.84 RR75 pKa = 11.84 NN76 pKa = 3.01
Molecular weight: 8.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.49
IPC2_protein 11.082
IPC_protein 12.705
Toseland 12.866
ProMoST 13.364
Dawson 12.866
Bjellqvist 12.866
Wikipedia 13.349
Rodwell 12.369
Grimsley 12.91
Solomon 13.364
Lehninger 13.261
Nozaki 12.866
DTASelect 12.866
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.091
IPC_peptide 13.364
IPC2_peptide 12.354
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4522
0
4522
1433990
28
2028
317.1
34.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.117 ± 0.062
0.831 ± 0.012
5.59 ± 0.028
6.108 ± 0.037
3.549 ± 0.025
9.38 ± 0.044
1.912 ± 0.017
4.722 ± 0.025
2.361 ± 0.029
10.334 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.017
2.13 ± 0.017
5.725 ± 0.027
2.347 ± 0.021
8.18 ± 0.039
4.625 ± 0.028
4.996 ± 0.025
7.175 ± 0.03
1.428 ± 0.014
2.095 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here