Rhodovastum atsumiense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Rhodovastum

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5M6IQN4|A0A5M6IQN4_9PROT Lrp/AsnC family transcriptional regulator OS=Rhodovastum atsumiense OX=504468 GN=F1189_20505 PE=4 SV=1
MM1 pKa = 7.75AIIPEE6 pKa = 5.18DD7 pKa = 3.32ITSIDD12 pKa = 3.93LGALSLPSIDD22 pKa = 4.62FGNPGANVSNAAGQLGTALQALWLRR47 pKa = 11.84NGGGTGASVEE57 pKa = 4.39SLRR60 pKa = 11.84AALPAVDD67 pKa = 4.23PAPVAGARR75 pKa = 11.84YY76 pKa = 7.79PVLYY80 pKa = 10.74AFGDD84 pKa = 4.07SLSDD88 pKa = 3.39TGNDD92 pKa = 3.08QATTGGIVPVAPYY105 pKa = 11.19ADD107 pKa = 4.13GNFSNGPVWVEE118 pKa = 3.51YY119 pKa = 10.63LAGQLGLPLPQPSLQGGGNFAFGGAQTGATTVHH152 pKa = 6.08QEE154 pKa = 4.15TPLDD158 pKa = 4.03LPSQLLQFRR167 pKa = 11.84AQVQAPNPDD176 pKa = 3.37ALYY179 pKa = 9.62TLSIGGNDD187 pKa = 3.54MLAVAGIAGSDD198 pKa = 3.58PASAQQAVTQAVGNVTQFVSGLADD222 pKa = 3.17SGARR226 pKa = 11.84NILILNVPDD235 pKa = 4.88LGHH238 pKa = 6.59TPDD241 pKa = 3.43QAAAGPAQAGTASSLAAQFNQQLQQALGEE270 pKa = 4.25LEE272 pKa = 3.96QTRR275 pKa = 11.84QLDD278 pKa = 3.16IDD280 pKa = 4.33LVDD283 pKa = 3.58TYY285 pKa = 11.98GLLDD289 pKa = 3.59QAATNPAAFGFANVDD304 pKa = 3.38QPVYY308 pKa = 10.83SGGPFPNSGGTLQATGTAQNQYY330 pKa = 11.24LFFDD334 pKa = 4.1TLHH337 pKa = 6.45PTTTGHH343 pKa = 7.6ALLAGNAVQALAA355 pKa = 4.42

Molecular weight: 35.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5M6IY35|A0A5M6IY35_9PROT YdcF family protein OS=Rhodovastum atsumiense OX=504468 GN=F1189_06135 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.09VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.85GRR39 pKa = 11.84KK40 pKa = 8.87RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6206

0

6206

2055468

25

3097

331.2

35.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.402 ± 0.052

0.98 ± 0.01

5.178 ± 0.02

5.214 ± 0.029

3.238 ± 0.018

9.057 ± 0.028

2.111 ± 0.014

4.296 ± 0.022

1.896 ± 0.02

10.908 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.202 ± 0.013

2.019 ± 0.016

6.063 ± 0.033

3.189 ± 0.02

8.425 ± 0.032

4.578 ± 0.023

5.322 ± 0.024

7.637 ± 0.025

1.473 ± 0.012

1.811 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski