Ideonella sp. TBM-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Ideonella; unclassified Ideonella

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4542 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7C9TK73|A0A7C9TK73_9BURK Protein-glutamate methylesterase/protein-glutamine glutaminase OS=Ideonella sp. TBM-1 OX=2707176 GN=cheB PE=3 SV=1
MM1 pKa = 7.86LAGCSDD7 pKa = 3.67NGDD10 pKa = 3.95PGTSVFDD17 pKa = 5.2DD18 pKa = 4.08EE19 pKa = 5.72DD20 pKa = 4.43SSASSTTTDD29 pKa = 3.4DD30 pKa = 3.15ASYY33 pKa = 8.89TVAVAVLASGVEE45 pKa = 4.22VSSVSTSTTSVQLSATVTDD64 pKa = 4.01ADD66 pKa = 3.96GAPVSGVVVSFSDD79 pKa = 3.99DD80 pKa = 3.48RR81 pKa = 11.84GLLSYY86 pKa = 10.57SPSVATALTGDD97 pKa = 4.46DD98 pKa = 4.36GVALLDD104 pKa = 3.72VTPASAGATTLTATATVDD122 pKa = 2.96ASSYY126 pKa = 11.17SGTATLAVTEE136 pKa = 4.49SAGTTTVQLGSVATRR151 pKa = 11.84VVVTATAAAHH161 pKa = 6.57ASVTVNSDD169 pKa = 3.09MQSISTRR176 pKa = 11.84STTLTAYY183 pKa = 10.63LSNANGHH190 pKa = 6.1PVADD194 pKa = 4.27GVAVSFTTDD203 pKa = 3.69DD204 pKa = 3.74GSSTLSSTLQIHH216 pKa = 6.4RR217 pKa = 11.84AAACGG222 pKa = 3.26

Molecular weight: 21.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7C9TK01|A0A7C9TK01_9BURK Molybdopterin oxidoreductase family protein OS=Ideonella sp. TBM-1 OX=2707176 GN=G3A44_14415 PE=3 SV=1
MM1 pKa = 7.41SRR3 pKa = 11.84LAGLARR9 pKa = 11.84ARR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84LQASRR18 pKa = 11.84PGFLSVTAVAAGLRR32 pKa = 11.84IRR34 pKa = 11.84HH35 pKa = 5.47ARR37 pKa = 11.84QVARR41 pKa = 11.84LRR43 pKa = 11.84PAIARR48 pKa = 11.84LRR50 pKa = 3.82

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4542

0

4542

1579378

35

5714

347.7

37.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.084 ± 0.07

0.942 ± 0.013

5.162 ± 0.052

5.0 ± 0.034

2.998 ± 0.022

8.945 ± 0.072

2.426 ± 0.02

3.122 ± 0.03

2.387 ± 0.035

11.77 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.018

2.018 ± 0.032

6.043 ± 0.044

4.634 ± 0.038

7.367 ± 0.047

4.763 ± 0.032

5.121 ± 0.046

7.601 ± 0.031

1.704 ± 0.02

1.824 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski