Amino acid dipepetide frequency for Ideonella sp. TBM-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.227AlaAla: 21.227 ± 0.217
1.546AlaCys: 1.546 ± 0.037
7.005AlaAsp: 7.005 ± 0.08
7.312AlaGlu: 7.312 ± 0.082
3.632AlaPhe: 3.632 ± 0.056
12.942AlaGly: 12.942 ± 0.156
3.009AlaHis: 3.009 ± 0.055
3.684AlaIle: 3.684 ± 0.052
3.004AlaLys: 3.004 ± 0.059
18.196AlaLeu: 18.196 ± 0.184
3.158AlaMet: 3.158 ± 0.044
2.251AlaAsn: 2.251 ± 0.05
8.209AlaPro: 8.209 ± 0.103
7.492AlaGln: 7.492 ± 0.101
10.344AlaArg: 10.344 ± 0.105
6.412AlaSer: 6.412 ± 0.069
6.687AlaThr: 6.687 ± 0.072
9.786AlaVal: 9.786 ± 0.084
2.629AlaTrp: 2.629 ± 0.052
2.314AlaTyr: 2.314 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
1.218CysAla: 1.218 ± 0.031
0.133CysCys: 0.133 ± 0.011
0.486CysAsp: 0.486 ± 0.018
0.467CysGlu: 0.467 ± 0.018
0.287CysPhe: 0.287 ± 0.011
1.053CysGly: 1.053 ± 0.032
0.283CysHis: 0.283 ± 0.015
0.302CysIle: 0.302 ± 0.014
0.172CysLys: 0.172 ± 0.012
1.014CysLeu: 1.014 ± 0.026
0.181CysMet: 0.181 ± 0.012
0.212CysAsn: 0.212 ± 0.012
0.591CysPro: 0.591 ± 0.021
0.376CysGln: 0.376 ± 0.016
0.675CysArg: 0.675 ± 0.022
0.448CysSer: 0.448 ± 0.019
0.517CysThr: 0.517 ± 0.021
0.674CysVal: 0.674 ± 0.022
0.156CysTrp: 0.156 ± 0.011
0.17CysTyr: 0.17 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.933AspAla: 6.933 ± 0.073
0.427AspCys: 0.427 ± 0.017
2.803AspAsp: 2.803 ± 0.119
2.924AspGlu: 2.924 ± 0.054
1.851AspPhe: 1.851 ± 0.032
5.022AspGly: 5.022 ± 0.133
1.188AspHis: 1.188 ± 0.029
1.824AspIle: 1.824 ± 0.035
1.277AspLys: 1.277 ± 0.038
6.096AspLeu: 6.096 ± 0.072
1.061AspMet: 1.061 ± 0.022
0.963AspAsn: 0.963 ± 0.03
3.387AspPro: 3.387 ± 0.045
1.772AspGln: 1.772 ± 0.045
3.356AspArg: 3.356 ± 0.057
2.086AspSer: 2.086 ± 0.085
2.923AspThr: 2.923 ± 0.144
3.477AspVal: 3.477 ± 0.05
1.124AspTrp: 1.124 ± 0.029
1.122AspTyr: 1.122 ± 0.026
0.0AspXaa: 0.0 ± 0.0
Glu
7.687GluAla: 7.687 ± 0.09
0.344GluCys: 0.344 ± 0.016
2.353GluAsp: 2.353 ± 0.039
2.376GluGlu: 2.376 ± 0.055
1.459GluPhe: 1.459 ± 0.035
4.133GluGly: 4.133 ± 0.075
1.259GluHis: 1.259 ± 0.027
1.909GluIle: 1.909 ± 0.037
1.266GluLys: 1.266 ± 0.037
5.697GluLeu: 5.697 ± 0.07
1.003GluMet: 1.003 ± 0.026
0.945GluAsn: 0.945 ± 0.025
2.569GluPro: 2.569 ± 0.047
2.578GluGln: 2.578 ± 0.045
4.589GluArg: 4.589 ± 0.071
1.95GluSer: 1.95 ± 0.04
2.208GluThr: 2.208 ± 0.04
4.302GluVal: 4.302 ± 0.06
0.626GluTrp: 0.626 ± 0.023
0.749GluTyr: 0.749 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
3.547PheAla: 3.547 ± 0.055
0.353PheCys: 0.353 ± 0.017
2.063PheAsp: 2.063 ± 0.037
1.836PheGlu: 1.836 ± 0.034
1.113PhePhe: 1.113 ± 0.031
2.865PheGly: 2.865 ± 0.048
0.734PheHis: 0.734 ± 0.02
1.211PheIle: 1.211 ± 0.034
0.924PheLys: 0.924 ± 0.023
2.691PheLeu: 2.691 ± 0.045
0.73PheMet: 0.73 ± 0.023
0.875PheAsn: 0.875 ± 0.025
1.25PhePro: 1.25 ± 0.03
1.068PheGln: 1.068 ± 0.02
1.726PheArg: 1.726 ± 0.034
1.825PheSer: 1.825 ± 0.035
1.815PheThr: 1.815 ± 0.04
2.194PheVal: 2.194 ± 0.045
0.488PheTrp: 0.488 ± 0.02
0.671PheTyr: 0.671 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
10.61GlyAla: 10.61 ± 0.128
0.958GlyCys: 0.958 ± 0.025
4.157GlyAsp: 4.157 ± 0.154
4.558GlyGlu: 4.558 ± 0.075
2.901GlyPhe: 2.901 ± 0.043
8.268GlyGly: 8.268 ± 0.218
2.384GlyHis: 2.384 ± 0.043
2.842GlyIle: 2.842 ± 0.046
2.431GlyLys: 2.431 ± 0.051
11.307GlyLeu: 11.307 ± 0.106
2.088GlyMet: 2.088 ± 0.041
1.935GlyAsn: 1.935 ± 0.124
4.285GlyPro: 4.285 ± 0.054
4.701GlyGln: 4.701 ± 0.064
6.507GlyArg: 6.507 ± 0.08
4.254GlySer: 4.254 ± 0.065
4.715GlyThr: 4.715 ± 0.098
6.788GlyVal: 6.788 ± 0.065
1.825GlyTrp: 1.825 ± 0.04
1.941GlyTyr: 1.941 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
3.14HisAla: 3.14 ± 0.05
0.305HisCys: 0.305 ± 0.014
1.303HisAsp: 1.303 ± 0.034
1.166HisGlu: 1.166 ± 0.031
0.874HisPhe: 0.874 ± 0.022
2.379HisGly: 2.379 ± 0.04
0.819HisHis: 0.819 ± 0.029
0.767HisIle: 0.767 ± 0.029
0.45HisLys: 0.45 ± 0.017
2.896HisLeu: 2.896 ± 0.045
0.404HisMet: 0.404 ± 0.016
0.433HisAsn: 0.433 ± 0.018
1.813HisPro: 1.813 ± 0.042
0.954HisGln: 0.954 ± 0.025
1.839HisArg: 1.839 ± 0.033
0.908HisSer: 0.908 ± 0.028
1.141HisThr: 1.141 ± 0.026
1.544HisVal: 1.544 ± 0.033
0.545HisTrp: 0.545 ± 0.02
0.582HisTyr: 0.582 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
4.223IleAla: 4.223 ± 0.062
0.299IleCys: 0.299 ± 0.015
2.196IleAsp: 2.196 ± 0.038
2.188IleGlu: 2.188 ± 0.044
0.855IlePhe: 0.855 ± 0.025
3.066IleGly: 3.066 ± 0.051
0.714IleHis: 0.714 ± 0.021
0.967IleIle: 0.967 ± 0.03
0.938IleLys: 0.938 ± 0.03
2.56IleLeu: 2.56 ± 0.04
0.503IleMet: 0.503 ± 0.019
0.904IleAsn: 0.904 ± 0.026
1.489IlePro: 1.489 ± 0.033
1.123IleGln: 1.123 ± 0.026
2.034IleArg: 2.034 ± 0.034
1.664IleSer: 1.664 ± 0.037
2.062IleThr: 2.062 ± 0.046
2.482IleVal: 2.482 ± 0.051
0.351IleTrp: 0.351 ± 0.017
0.6IleTyr: 0.6 ± 0.019
0.0IleXaa: 0.0 ± 0.0
Lys
3.348LysAla: 3.348 ± 0.069
0.138LysCys: 0.138 ± 0.01
1.191LysAsp: 1.191 ± 0.033
1.043LysGlu: 1.043 ± 0.033
0.688LysPhe: 0.688 ± 0.024
2.006LysGly: 2.006 ± 0.043
0.444LysHis: 0.444 ± 0.018
0.952LysIle: 0.952 ± 0.031
0.917LysLys: 0.917 ± 0.038
2.607LysLeu: 2.607 ± 0.045
0.533LysMet: 0.533 ± 0.02
0.577LysAsn: 0.577 ± 0.019
1.532LysPro: 1.532 ± 0.04
0.79LysGln: 0.79 ± 0.026
1.542LysArg: 1.542 ± 0.038
1.263LysSer: 1.263 ± 0.035
1.387LysThr: 1.387 ± 0.035
2.152LysVal: 2.152 ± 0.048
0.27LysTrp: 0.27 ± 0.013
0.481LysTyr: 0.481 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
18.017LeuAla: 18.017 ± 0.137
1.118LeuCys: 1.118 ± 0.027
6.536LeuAsp: 6.536 ± 0.073
5.286LeuGlu: 5.286 ± 0.066
3.018LeuPhe: 3.018 ± 0.051
10.335LeuGly: 10.335 ± 0.109
2.784LeuHis: 2.784 ± 0.041
3.45LeuIle: 3.45 ± 0.053
3.028LeuLys: 3.028 ± 0.05
12.577LeuLeu: 12.577 ± 0.133
2.452LeuMet: 2.452 ± 0.047
2.424LeuAsn: 2.424 ± 0.037
7.593LeuPro: 7.593 ± 0.095
5.789LeuGln: 5.789 ± 0.08
9.241LeuArg: 9.241 ± 0.094
6.458LeuSer: 6.458 ± 0.085
5.761LeuThr: 5.761 ± 0.09
8.302LeuVal: 8.302 ± 0.093
1.654LeuTrp: 1.654 ± 0.04
1.878LeuTyr: 1.878 ± 0.036
0.0LeuXaa: 0.0 ± 0.0
Met
3.071MetAla: 3.071 ± 0.05
0.138MetCys: 0.138 ± 0.009
1.074MetAsp: 1.074 ± 0.027
0.835MetGlu: 0.835 ± 0.023
0.546MetPhe: 0.546 ± 0.019
1.737MetGly: 1.737 ± 0.031
0.473MetHis: 0.473 ± 0.019
0.69MetIle: 0.69 ± 0.023
0.688MetLys: 0.688 ± 0.023
2.06MetLeu: 2.06 ± 0.037
0.457MetMet: 0.457 ± 0.019
0.683MetAsn: 0.683 ± 0.022
1.383MetPro: 1.383 ± 0.03
0.86MetGln: 0.86 ± 0.026
1.454MetArg: 1.454 ± 0.027
1.446MetSer: 1.446 ± 0.031
1.333MetThr: 1.333 ± 0.028
1.531MetVal: 1.531 ± 0.033
0.186MetTrp: 0.186 ± 0.01
0.244MetTyr: 0.244 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.726AsnAla: 2.726 ± 0.047
0.201AsnCys: 0.201 ± 0.012
1.166AsnAsp: 1.166 ± 0.106
0.83AsnGlu: 0.83 ± 0.025
0.712AsnPhe: 0.712 ± 0.02
1.825AsnGly: 1.825 ± 0.049
0.459AsnHis: 0.459 ± 0.019
0.79AsnIle: 0.79 ± 0.022
0.498AsnLys: 0.498 ± 0.021
2.236AsnLeu: 2.236 ± 0.039
0.379AsnMet: 0.379 ± 0.015
0.457AsnAsn: 0.457 ± 0.02
1.575AsnPro: 1.575 ± 0.037
0.704AsnGln: 0.704 ± 0.02
1.371AsnArg: 1.371 ± 0.028
0.854AsnSer: 0.854 ± 0.026
1.117AsnThr: 1.117 ± 0.031
1.532AsnVal: 1.532 ± 0.035
0.294AsnTrp: 0.294 ± 0.014
0.455AsnTyr: 0.455 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
10.046ProAla: 10.046 ± 0.101
0.436ProCys: 0.436 ± 0.016
3.406ProAsp: 3.406 ± 0.046
3.67ProGlu: 3.67 ± 0.052
1.649ProPhe: 1.649 ± 0.036
5.928ProGly: 5.928 ± 0.082
1.337ProHis: 1.337 ± 0.032
1.351ProIle: 1.351 ± 0.03
1.138ProLys: 1.138 ± 0.035
6.485ProLeu: 6.485 ± 0.075
1.157ProMet: 1.157 ± 0.029
0.976ProAsn: 0.976 ± 0.028
3.942ProPro: 3.942 ± 0.068
2.718ProGln: 2.718 ± 0.047
3.581ProArg: 3.581 ± 0.061
2.728ProSer: 2.728 ± 0.048
2.916ProThr: 2.916 ± 0.045
4.869ProVal: 4.869 ± 0.071
1.096ProTrp: 1.096 ± 0.026
0.997ProTyr: 0.997 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
8.948GlnAla: 8.948 ± 0.104
0.332GlnCys: 0.332 ± 0.015
2.102GlnAsp: 2.102 ± 0.037
1.8GlnGlu: 1.8 ± 0.034
1.138GlnPhe: 1.138 ± 0.03
4.197GlnGly: 4.197 ± 0.052
1.069GlnHis: 1.069 ± 0.028
1.425GlnIle: 1.425 ± 0.032
0.875GlnLys: 0.875 ± 0.029
4.721GlnLeu: 4.721 ± 0.067
0.825GlnMet: 0.825 ± 0.026
0.623GlnAsn: 0.623 ± 0.02
2.947GlnPro: 2.947 ± 0.048
2.064GlnGln: 2.064 ± 0.043
4.096GlnArg: 4.096 ± 0.055
1.697GlnSer: 1.697 ± 0.035
2.088GlnThr: 2.088 ± 0.039
4.041GlnVal: 4.041 ± 0.068
0.747GlnTrp: 0.747 ± 0.025
0.608GlnTyr: 0.608 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
8.683ArgAla: 8.683 ± 0.095
0.692ArgCys: 0.692 ± 0.021
3.303ArgAsp: 3.303 ± 0.055
4.083ArgGlu: 4.083 ± 0.07
2.583ArgPhe: 2.583 ± 0.045
4.907ArgGly: 4.907 ± 0.064
2.473ArgHis: 2.473 ± 0.045
2.46ArgIle: 2.46 ± 0.043
1.544ArgLys: 1.544 ± 0.038
10.234ArgLeu: 10.234 ± 0.122
1.672ArgMet: 1.672 ± 0.036
1.351ArgAsn: 1.351 ± 0.029
4.395ArgPro: 4.395 ± 0.062
4.273ArgGln: 4.273 ± 0.062
6.135ArgArg: 6.135 ± 0.079
3.221ArgSer: 3.221 ± 0.049
3.218ArgThr: 3.218 ± 0.049
5.187ArgVal: 5.187 ± 0.059
1.681ArgTrp: 1.681 ± 0.041
1.579ArgTyr: 1.579 ± 0.033
0.0ArgXaa: 0.0 ± 0.0
Ser
6.508SerAla: 6.508 ± 0.079
0.415SerCys: 0.415 ± 0.017
2.069SerAsp: 2.069 ± 0.042
1.89SerGlu: 1.89 ± 0.036
1.661SerPhe: 1.661 ± 0.037
4.92SerGly: 4.92 ± 0.074
1.127SerHis: 1.127 ± 0.03
1.513SerIle: 1.513 ± 0.036
1.017SerLys: 1.017 ± 0.028
5.807SerLeu: 5.807 ± 0.066
0.949SerMet: 0.949 ± 0.027
0.955SerAsn: 0.955 ± 0.028
3.176SerPro: 3.176 ± 0.052
1.834SerGln: 1.834 ± 0.033
3.356SerArg: 3.356 ± 0.054
2.5SerSer: 2.5 ± 0.061
2.815SerThr: 2.815 ± 0.075
3.48SerVal: 3.48 ± 0.062
0.706SerTrp: 0.706 ± 0.02
0.938SerTyr: 0.938 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.592ThrAla: 6.592 ± 0.118
0.423ThrCys: 0.423 ± 0.019
2.474ThrAsp: 2.474 ± 0.07
2.296ThrGlu: 2.296 ± 0.039
1.473ThrPhe: 1.473 ± 0.031
5.094ThrGly: 5.094 ± 0.106
1.137ThrHis: 1.137 ± 0.027
1.335ThrIle: 1.335 ± 0.033
0.88ThrLys: 0.88 ± 0.026
7.12ThrLeu: 7.12 ± 0.096
0.773ThrMet: 0.773 ± 0.025
0.904ThrAsn: 0.904 ± 0.032
3.988ThrPro: 3.988 ± 0.057
2.031ThrGln: 2.031 ± 0.044
3.248ThrArg: 3.248 ± 0.048
2.252ThrSer: 2.252 ± 0.045
2.867ThrThr: 2.867 ± 0.099
4.59ThrVal: 4.59 ± 0.105
0.805ThrTrp: 0.805 ± 0.025
0.924ThrTyr: 0.924 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
10.143ValAla: 10.143 ± 0.109
0.753ValCys: 0.753 ± 0.021
4.197ValAsp: 4.197 ± 0.07
3.95ValGlu: 3.95 ± 0.054
2.378ValPhe: 2.378 ± 0.043
5.838ValGly: 5.838 ± 0.065
1.707ValHis: 1.707 ± 0.033
2.692ValIle: 2.692 ± 0.05
1.975ValLys: 1.975 ± 0.044
8.826ValLeu: 8.826 ± 0.084
1.858ValMet: 1.858 ± 0.036
1.893ValAsn: 1.893 ± 0.042
4.336ValPro: 4.336 ± 0.057
3.418ValGln: 3.418 ± 0.05
5.37ValArg: 5.37 ± 0.071
3.981ValSer: 3.981 ± 0.063
3.758ValThr: 3.758 ± 0.058
6.479ValVal: 6.479 ± 0.07
1.139ValTrp: 1.139 ± 0.027
1.32ValTyr: 1.32 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.91TrpAla: 1.91 ± 0.042
0.205TrpCys: 0.205 ± 0.012
0.709TrpAsp: 0.709 ± 0.026
0.589TrpGlu: 0.589 ± 0.021
0.477TrpPhe: 0.477 ± 0.016
1.222TrpGly: 1.222 ± 0.033
0.448TrpHis: 0.448 ± 0.018
0.52TrpIle: 0.52 ± 0.018
0.369TrpLys: 0.369 ± 0.016
2.453TrpLeu: 2.453 ± 0.047
0.394TrpMet: 0.394 ± 0.016
0.363TrpAsn: 0.363 ± 0.015
1.062TrpPro: 1.062 ± 0.03
1.015TrpGln: 1.015 ± 0.025
1.71TrpArg: 1.71 ± 0.04
0.822TrpSer: 0.822 ± 0.022
0.84TrpThr: 0.84 ± 0.026
1.264TrpVal: 1.264 ± 0.027
0.412TrpTrp: 0.412 ± 0.017
0.253TrpTyr: 0.253 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.262TyrAla: 2.262 ± 0.039
0.199TyrCys: 0.199 ± 0.01
1.023TyrAsp: 1.023 ± 0.026
0.904TyrGlu: 0.904 ± 0.022
0.683TyrPhe: 0.683 ± 0.025
1.717TyrGly: 1.717 ± 0.04
0.412TyrHis: 0.412 ± 0.017
0.534TyrIle: 0.534 ± 0.02
0.472TyrLys: 0.472 ± 0.019
2.117TyrLeu: 2.117 ± 0.04
0.312TyrMet: 0.312 ± 0.016
0.459TyrAsn: 0.459 ± 0.02
0.905TyrPro: 0.905 ± 0.025
0.784TyrGln: 0.784 ± 0.023
1.499TyrArg: 1.499 ± 0.037
0.869TyrSer: 0.869 ± 0.025
1.038TyrThr: 1.038 ± 0.031
1.337TyrVal: 1.337 ± 0.031
0.298TyrTrp: 0.298 ± 0.014
0.415TyrTyr: 0.415 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4542 proteins (1579378 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski