Vibrio phage VvAW1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3PV04|I3PV04_9CAUD SASA domain-containing protein OS=Vibrio phage VvAW1 OX=1168281 GN=VvAW1_00040c PE=4 SV=1
MM1 pKa = 7.69ANNLSMPRR9 pKa = 11.84FKK11 pKa = 11.17AFGQDD16 pKa = 3.38GKK18 pKa = 9.65PLAFGRR24 pKa = 11.84VYY26 pKa = 10.69LYY28 pKa = 10.55YY29 pKa = 10.2PRR31 pKa = 11.84TSEE34 pKa = 4.25KK35 pKa = 10.61KK36 pKa = 9.83PGYY39 pKa = 8.61TDD41 pKa = 2.93STANVEE47 pKa = 4.16LQNPVILDD55 pKa = 3.78ANGEE59 pKa = 4.0AAIFLDD65 pKa = 3.96GAYY68 pKa = 9.8KK69 pKa = 10.41IILRR73 pKa = 11.84DD74 pKa = 3.77KK75 pKa = 11.2NDD77 pKa = 3.31VLQWTVDD84 pKa = 3.43NYY86 pKa = 9.48NTDD89 pKa = 3.57YY90 pKa = 10.95FSSTNVSITPQANKK104 pKa = 10.02VPLAGSTGVITPGWIDD120 pKa = 3.53GLIAVYY126 pKa = 11.01NDD128 pKa = 3.03VAVSKK133 pKa = 11.31GDD135 pKa = 3.64IVLLKK140 pKa = 10.73QKK142 pKa = 10.43DD143 pKa = 3.74AEE145 pKa = 4.39HH146 pKa = 6.94DD147 pKa = 3.81TNISVLQTVTGDD159 pKa = 3.35NADD162 pKa = 4.7GITQLSIDD170 pKa = 3.77VQNLKK175 pKa = 10.4GASASLVKK183 pKa = 10.58GYY185 pKa = 8.32ATLAALNADD194 pKa = 4.79LVPEE198 pKa = 5.29DD199 pKa = 3.74GTLADD204 pKa = 3.91VTNDD208 pKa = 3.37PDD210 pKa = 3.9TNNNGRR216 pKa = 11.84YY217 pKa = 8.79RR218 pKa = 11.84KK219 pKa = 10.03SGATGTGSWVKK230 pKa = 9.82TNYY233 pKa = 10.19SEE235 pKa = 5.37WSTLWLEE242 pKa = 4.19MNRR245 pKa = 11.84LNDD248 pKa = 3.58FVYY251 pKa = 11.03DD252 pKa = 3.83LAIYY256 pKa = 10.0NYY258 pKa = 10.68NKK260 pKa = 8.75TQEE263 pKa = 4.2EE264 pKa = 4.69LEE266 pKa = 4.16IVPIVMDD273 pKa = 4.46EE274 pKa = 4.11ANKK277 pKa = 9.26MALGFYY283 pKa = 10.43SDD285 pKa = 4.29TGEE288 pKa = 3.73LWAAGIEE295 pKa = 4.2RR296 pKa = 11.84IEE298 pKa = 4.11ATGKK302 pKa = 9.67AVQPVVVNEE311 pKa = 4.32NGDD314 pKa = 3.38IAIGIEE320 pKa = 4.29SEE322 pKa = 4.23TGQTMMDD329 pKa = 3.35PHH331 pKa = 7.81YY332 pKa = 9.38STIMYY337 pKa = 9.08GVKK340 pKa = 10.11KK341 pKa = 10.77VGIFPDD347 pKa = 3.59EE348 pKa = 4.44FNVKK352 pKa = 10.1DD353 pKa = 4.05GYY355 pKa = 11.41VYY357 pKa = 10.83AIVDD361 pKa = 4.0EE362 pKa = 4.54DD363 pKa = 3.64KK364 pKa = 11.16KK365 pKa = 10.81IAFGIKK371 pKa = 9.84DD372 pKa = 3.91DD373 pKa = 4.06GSLVGSGLTFQTAPVEE389 pKa = 4.37KK390 pKa = 10.13GDD392 pKa = 3.72KK393 pKa = 10.25QSIDD397 pKa = 3.31MEE399 pKa = 4.75HH400 pKa = 6.03NHH402 pKa = 7.0FIFSGQSLSVGATGKK417 pKa = 9.7PVISTSQPYY426 pKa = 11.03DD427 pKa = 3.15NLTFDD432 pKa = 4.19GGPRR436 pKa = 11.84SDD438 pKa = 4.64LDD440 pKa = 3.57EE441 pKa = 4.31LTGFKK446 pKa = 10.5PLVEE450 pKa = 5.13DD451 pKa = 4.43EE452 pKa = 4.83NSPAPDD458 pKa = 3.49GGTNRR463 pKa = 11.84GEE465 pKa = 4.47TCCSGAANFTTHH477 pKa = 7.36LIEE480 pKa = 5.75SEE482 pKa = 4.16DD483 pKa = 4.02GIAYY487 pKa = 8.06DD488 pKa = 3.42QQGYY492 pKa = 10.37AIVSSTVGHH501 pKa = 6.15GGYY504 pKa = 10.36RR505 pKa = 11.84IDD507 pKa = 3.69QLYY510 pKa = 10.22KK511 pKa = 9.45GSAWYY516 pKa = 9.19DD517 pKa = 3.7YY518 pKa = 9.79YY519 pKa = 10.59TAHH522 pKa = 5.8VQAGFDD528 pKa = 3.59LSQAQGKK535 pKa = 7.12TYY537 pKa = 10.71AVQALGWIQGEE548 pKa = 3.8NDD550 pKa = 3.08QFDD553 pKa = 3.64NSKK556 pKa = 10.95ARR558 pKa = 11.84LAYY561 pKa = 9.89KK562 pKa = 10.35NDD564 pKa = 4.8LIQYY568 pKa = 8.51QADD571 pKa = 3.55IEE573 pKa = 4.66ADD575 pKa = 3.31AQAITGQTHH584 pKa = 6.31RR585 pKa = 11.84VPLLTYY591 pKa = 10.45QLACYY596 pKa = 9.26VRR598 pKa = 11.84QGYY601 pKa = 10.52RR602 pKa = 11.84NVTLAQLDD610 pKa = 4.06CHH612 pKa = 7.14KK613 pKa = 10.07EE614 pKa = 3.93SQDD617 pKa = 3.19IYY619 pKa = 11.01LVTPMYY625 pKa = 9.88HH626 pKa = 5.61VPYY629 pKa = 9.61ATDD632 pKa = 3.77AVHH635 pKa = 6.33LTNLGYY641 pKa = 10.84LWLGHH646 pKa = 5.74YY647 pKa = 9.53FGKK650 pKa = 10.58VYY652 pKa = 10.66KK653 pKa = 10.38KK654 pKa = 10.74VVFDD658 pKa = 4.55GIEE661 pKa = 3.91WEE663 pKa = 4.14PVRR666 pKa = 11.84PKK668 pKa = 10.54TITQQGAVVLIDD680 pKa = 4.27FFVPDD685 pKa = 5.09PPLQLDD691 pKa = 3.94TSTLGLATDD700 pKa = 4.14YY701 pKa = 11.54GFAAYY706 pKa = 9.78DD707 pKa = 3.49DD708 pKa = 4.6SGDD711 pKa = 3.46IDD713 pKa = 4.02VLSVEE718 pKa = 5.09LVGQTRR724 pKa = 11.84VKK726 pKa = 9.79LTLASAPGAGGGIRR740 pKa = 11.84YY741 pKa = 9.42GLDD744 pKa = 3.4YY745 pKa = 10.89IGTGLTHH752 pKa = 6.84NNGGSGNLRR761 pKa = 11.84DD762 pKa = 3.83SAPEE766 pKa = 3.78YY767 pKa = 9.29FTYY770 pKa = 10.81AGTDD774 pKa = 3.38YY775 pKa = 10.93PLYY778 pKa = 8.87NWCVMFDD785 pKa = 3.61EE786 pKa = 6.08LII788 pKa = 4.26

Molecular weight: 86.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3PUZ0|I3PUZ0_9CAUD RNA-binding regulator protein OS=Vibrio phage VvAW1 OX=1168281 GN=VvAW1_00026c PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.11AKK5 pKa = 9.93PVDD8 pKa = 3.85VFGVRR13 pKa = 11.84YY14 pKa = 9.06KK15 pKa = 10.62SKK17 pKa = 10.12RR18 pKa = 11.84QACIAHH24 pKa = 5.93GQNPGKK30 pKa = 9.54IDD32 pKa = 3.23HH33 pKa = 7.19RR34 pKa = 11.84IAKK37 pKa = 9.9GMTLEE42 pKa = 4.85DD43 pKa = 3.32ALKK46 pKa = 10.96AKK48 pKa = 10.41DD49 pKa = 3.77RR50 pKa = 11.84YY51 pKa = 8.5TGAKK55 pKa = 8.61SHH57 pKa = 7.18PSYY60 pKa = 9.49THH62 pKa = 5.88WNGIRR67 pKa = 11.84SRR69 pKa = 11.84CLSDD73 pKa = 3.77SEE75 pKa = 4.21CSKK78 pKa = 10.37RR79 pKa = 11.84YY80 pKa = 9.83KK81 pKa = 10.7GVVDD85 pKa = 3.62ICDD88 pKa = 3.08RR89 pKa = 11.84WASDD93 pKa = 3.05FWSFVEE99 pKa = 5.98DD100 pKa = 3.11MGEE103 pKa = 3.98PPFVGATIEE112 pKa = 4.31RR113 pKa = 11.84LDD115 pKa = 3.52NSKK118 pKa = 11.07GYY120 pKa = 10.59DD121 pKa = 3.35KK122 pKa = 10.92EE123 pKa = 4.11NCIWASMKK131 pKa = 10.59DD132 pKa = 3.22QARR135 pKa = 11.84NRR137 pKa = 11.84RR138 pKa = 11.84TNVVIEE144 pKa = 4.4CFGEE148 pKa = 4.59TMCLADD154 pKa = 3.57WAVKK158 pKa = 9.63TGISSSLIAYY168 pKa = 7.94RR169 pKa = 11.84ISKK172 pKa = 9.33GWDD175 pKa = 2.89VEE177 pKa = 4.19RR178 pKa = 11.84ALTLKK183 pKa = 10.34PKK185 pKa = 10.31RR186 pKa = 11.84GRR188 pKa = 11.84NHH190 pKa = 5.57VNKK193 pKa = 10.5SS194 pKa = 3.03

Molecular weight: 21.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

12572

62

3640

314.3

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.64 ± 0.46

0.628 ± 0.196

6.753 ± 0.28

7.564 ± 0.476

3.253 ± 0.214

7.652 ± 0.347

1.631 ± 0.208

5.234 ± 0.222

6.554 ± 0.364

7.222 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.168

4.184 ± 0.199

4.208 ± 0.22

5.393 ± 0.361

5.433 ± 0.402

5.409 ± 0.378

5.608 ± 0.282

6.244 ± 0.296

1.456 ± 0.193

3.023 ± 0.257
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski