Amino acid dipepetide frequency for Vibrio phage VvAW1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.103AlaAla: 10.103 ± 1.24
0.955AlaCys: 0.955 ± 0.494
5.887AlaAsp: 5.887 ± 0.856
9.228AlaGlu: 9.228 ± 1.651
3.102AlaPhe: 3.102 ± 0.378
6.125AlaGly: 6.125 ± 1.115
2.068AlaHis: 2.068 ± 0.336
5.091AlaIle: 5.091 ± 0.71
6.762AlaLys: 6.762 ± 1.197
7.796AlaLeu: 7.796 ± 0.845
3.421AlaMet: 3.421 ± 0.473
4.852AlaAsn: 4.852 ± 0.78
3.898AlaPro: 3.898 ± 0.408
5.012AlaGln: 5.012 ± 1.0
4.932AlaArg: 4.932 ± 0.782
4.216AlaSer: 4.216 ± 0.631
4.375AlaThr: 4.375 ± 0.507
4.614AlaVal: 4.614 ± 0.757
1.193AlaTrp: 1.193 ± 0.318
2.784AlaTyr: 2.784 ± 0.57
0.0AlaXaa: 0.0 ± 0.0
Cys
0.636CysAla: 0.636 ± 0.346
0.08CysCys: 0.08 ± 0.091
0.636CysAsp: 0.636 ± 0.329
0.239CysGlu: 0.239 ± 0.171
0.318CysPhe: 0.318 ± 0.177
0.318CysGly: 0.318 ± 0.26
0.398CysHis: 0.398 ± 0.347
0.875CysIle: 0.875 ± 0.447
0.239CysLys: 0.239 ± 0.168
0.318CysLeu: 0.318 ± 0.237
0.239CysMet: 0.239 ± 0.178
0.159CysAsn: 0.159 ± 0.166
0.159CysPro: 0.159 ± 0.141
0.477CysGln: 0.477 ± 0.238
0.08CysArg: 0.08 ± 0.102
0.159CysSer: 0.159 ± 0.118
0.239CysThr: 0.239 ± 0.156
0.398CysVal: 0.398 ± 0.272
0.08CysTrp: 0.08 ± 0.085
0.239CysTyr: 0.239 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
7.318AspAla: 7.318 ± 0.759
0.398AspCys: 0.398 ± 0.221
4.455AspAsp: 4.455 ± 0.945
5.25AspGlu: 5.25 ± 0.938
2.625AspPhe: 2.625 ± 0.466
6.125AspGly: 6.125 ± 0.902
1.034AspHis: 1.034 ± 0.437
3.818AspIle: 3.818 ± 0.638
3.58AspLys: 3.58 ± 0.62
5.091AspLeu: 5.091 ± 0.703
2.466AspMet: 2.466 ± 0.435
2.466AspAsn: 2.466 ± 0.481
2.625AspPro: 2.625 ± 0.487
4.455AspGln: 4.455 ± 0.52
3.182AspArg: 3.182 ± 0.496
3.182AspSer: 3.182 ± 0.609
3.261AspThr: 3.261 ± 0.487
2.943AspVal: 2.943 ± 0.449
1.193AspTrp: 1.193 ± 0.392
2.068AspTyr: 2.068 ± 0.574
0.0AspXaa: 0.0 ± 0.0
Glu
8.114GluAla: 8.114 ± 1.171
0.557GluCys: 0.557 ± 0.34
4.296GluAsp: 4.296 ± 0.429
4.534GluGlu: 4.534 ± 0.888
2.943GluPhe: 2.943 ± 0.383
5.25GluGly: 5.25 ± 0.814
1.511GluHis: 1.511 ± 0.387
3.102GluIle: 3.102 ± 0.533
4.216GluLys: 4.216 ± 0.551
6.921GluLeu: 6.921 ± 0.726
2.307GluMet: 2.307 ± 0.481
2.386GluAsn: 2.386 ± 0.406
2.943GluPro: 2.943 ± 0.524
5.25GluGln: 5.25 ± 0.8
5.966GluArg: 5.966 ± 0.974
3.261GluSer: 3.261 ± 0.524
3.182GluThr: 3.182 ± 0.557
5.091GluVal: 5.091 ± 0.632
1.114GluTrp: 1.114 ± 0.336
2.705GluTyr: 2.705 ± 0.458
0.0GluXaa: 0.0 ± 0.0
Phe
2.864PheAla: 2.864 ± 0.604
0.08PheCys: 0.08 ± 0.102
3.421PheAsp: 3.421 ± 0.596
2.307PheGlu: 2.307 ± 0.419
0.636PhePhe: 0.636 ± 0.184
2.943PheGly: 2.943 ± 0.5
0.477PheHis: 0.477 ± 0.281
1.83PheIle: 1.83 ± 0.471
1.83PheLys: 1.83 ± 0.368
1.75PheLeu: 1.75 ± 0.418
0.636PheMet: 0.636 ± 0.229
1.352PheAsn: 1.352 ± 0.349
0.795PhePro: 0.795 ± 0.302
1.591PheGln: 1.591 ± 0.323
1.352PheArg: 1.352 ± 0.339
1.909PheSer: 1.909 ± 0.394
2.546PheThr: 2.546 ± 0.529
2.068PheVal: 2.068 ± 0.401
0.636PheTrp: 0.636 ± 0.231
1.511PheTyr: 1.511 ± 0.318
0.0PheXaa: 0.0 ± 0.0
Gly
6.364GlyAla: 6.364 ± 0.911
0.318GlyCys: 0.318 ± 0.24
4.375GlyAsp: 4.375 ± 0.51
4.693GlyGlu: 4.693 ± 0.513
2.386GlyPhe: 2.386 ± 0.367
5.727GlyGly: 5.727 ± 0.889
1.193GlyHis: 1.193 ± 0.334
4.773GlyIle: 4.773 ± 0.567
6.523GlyLys: 6.523 ± 0.907
4.375GlyLeu: 4.375 ± 0.516
1.989GlyMet: 1.989 ± 0.434
3.659GlyAsn: 3.659 ± 0.582
1.989GlyPro: 1.989 ± 0.323
4.693GlyGln: 4.693 ± 0.728
3.977GlyArg: 3.977 ± 0.567
4.455GlySer: 4.455 ± 0.666
5.33GlyThr: 5.33 ± 0.99
4.932GlyVal: 4.932 ± 0.62
1.432GlyTrp: 1.432 ± 0.286
3.341GlyTyr: 3.341 ± 0.751
0.0GlyXaa: 0.0 ± 0.0
His
0.557HisAla: 0.557 ± 0.18
0.08HisCys: 0.08 ± 0.099
1.114HisAsp: 1.114 ± 0.434
1.193HisGlu: 1.193 ± 0.345
0.557HisPhe: 0.557 ± 0.259
1.273HisGly: 1.273 ± 0.312
0.398HisHis: 0.398 ± 0.168
0.795HisIle: 0.795 ± 0.284
0.955HisLys: 0.955 ± 0.341
1.432HisLeu: 1.432 ± 0.351
0.636HisMet: 0.636 ± 0.296
0.955HisAsn: 0.955 ± 0.323
0.557HisPro: 0.557 ± 0.232
0.875HisGln: 0.875 ± 0.327
1.352HisArg: 1.352 ± 0.451
0.318HisSer: 0.318 ± 0.151
0.636HisThr: 0.636 ± 0.36
1.114HisVal: 1.114 ± 0.36
0.557HisTrp: 0.557 ± 0.246
0.955HisTyr: 0.955 ± 0.288
0.0HisXaa: 0.0 ± 0.0
Ile
4.693IleAla: 4.693 ± 0.569
0.398IleCys: 0.398 ± 0.239
4.614IleAsp: 4.614 ± 0.517
5.171IleGlu: 5.171 ± 0.595
1.273IlePhe: 1.273 ± 0.349
3.421IleGly: 3.421 ± 0.397
0.795IleHis: 0.795 ± 0.205
2.227IleIle: 2.227 ± 0.464
3.58IleLys: 3.58 ± 0.664
2.386IleLeu: 2.386 ± 0.535
1.75IleMet: 1.75 ± 0.476
3.421IleAsn: 3.421 ± 0.682
2.784IlePro: 2.784 ± 0.451
1.989IleGln: 1.989 ± 0.378
2.705IleArg: 2.705 ± 0.518
3.341IleSer: 3.341 ± 0.813
3.58IleThr: 3.58 ± 0.727
2.546IleVal: 2.546 ± 0.59
0.716IleTrp: 0.716 ± 0.348
0.875IleTyr: 0.875 ± 0.276
0.0IleXaa: 0.0 ± 0.0
Lys
7.478LysAla: 7.478 ± 0.909
0.398LysCys: 0.398 ± 0.279
4.137LysAsp: 4.137 ± 0.623
4.534LysGlu: 4.534 ± 0.862
1.909LysPhe: 1.909 ± 0.347
5.171LysGly: 5.171 ± 0.681
0.557LysHis: 0.557 ± 0.223
2.386LysIle: 2.386 ± 0.373
3.5LysLys: 3.5 ± 0.716
5.648LysLeu: 5.648 ± 0.928
1.352LysMet: 1.352 ± 0.36
2.307LysAsn: 2.307 ± 0.461
3.977LysPro: 3.977 ± 0.533
3.739LysGln: 3.739 ± 0.638
4.693LysArg: 4.693 ± 0.717
3.421LysSer: 3.421 ± 0.579
3.659LysThr: 3.659 ± 0.556
4.614LysVal: 4.614 ± 0.562
0.795LysTrp: 0.795 ± 0.225
1.273LysTyr: 1.273 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
7.716LeuAla: 7.716 ± 0.618
0.477LeuCys: 0.477 ± 0.269
5.012LeuAsp: 5.012 ± 0.551
4.852LeuGlu: 4.852 ± 0.655
2.386LeuPhe: 2.386 ± 0.534
5.648LeuGly: 5.648 ± 0.713
0.955LeuHis: 0.955 ± 0.293
3.898LeuIle: 3.898 ± 0.511
4.296LeuLys: 4.296 ± 0.627
4.296LeuLeu: 4.296 ± 0.66
2.307LeuMet: 2.307 ± 0.388
2.307LeuAsn: 2.307 ± 0.385
2.546LeuPro: 2.546 ± 0.631
3.421LeuGln: 3.421 ± 0.637
3.818LeuArg: 3.818 ± 0.635
5.171LeuSer: 5.171 ± 0.553
5.568LeuThr: 5.568 ± 0.688
4.296LeuVal: 4.296 ± 0.718
1.193LeuTrp: 1.193 ± 0.322
2.068LeuTyr: 2.068 ± 0.512
0.0LeuXaa: 0.0 ± 0.0
Met
3.739MetAla: 3.739 ± 0.52
0.08MetCys: 0.08 ± 0.095
2.307MetAsp: 2.307 ± 0.364
1.511MetGlu: 1.511 ± 0.391
0.795MetPhe: 0.795 ± 0.259
2.705MetGly: 2.705 ± 0.537
0.557MetHis: 0.557 ± 0.189
0.875MetIle: 0.875 ± 0.266
1.83MetLys: 1.83 ± 0.358
2.227MetLeu: 2.227 ± 0.356
0.716MetMet: 0.716 ± 0.202
0.875MetAsn: 0.875 ± 0.271
1.671MetPro: 1.671 ± 0.27
1.034MetGln: 1.034 ± 0.358
1.114MetArg: 1.114 ± 0.356
1.83MetSer: 1.83 ± 0.51
1.909MetThr: 1.909 ± 0.391
1.83MetVal: 1.83 ± 0.419
0.477MetTrp: 0.477 ± 0.195
1.034MetTyr: 1.034 ± 0.277
0.0MetXaa: 0.0 ± 0.0
Asn
4.773AsnAla: 4.773 ± 0.996
0.159AsnCys: 0.159 ± 0.131
2.068AsnAsp: 2.068 ± 0.655
3.5AsnGlu: 3.5 ± 0.46
1.114AsnPhe: 1.114 ± 0.29
3.421AsnGly: 3.421 ± 0.958
0.477AsnHis: 0.477 ± 0.192
1.83AsnIle: 1.83 ± 0.275
3.58AsnLys: 3.58 ± 0.423
3.023AsnLeu: 3.023 ± 0.701
1.114AsnMet: 1.114 ± 0.294
1.989AsnAsn: 1.989 ± 0.488
1.989AsnPro: 1.989 ± 0.505
2.705AsnGln: 2.705 ± 0.574
2.227AsnArg: 2.227 ± 0.389
1.83AsnSer: 1.83 ± 0.429
1.75AsnThr: 1.75 ± 0.494
2.546AsnVal: 2.546 ± 0.426
0.716AsnTrp: 0.716 ± 0.216
1.034AsnTyr: 1.034 ± 0.293
0.0AsnXaa: 0.0 ± 0.0
Pro
3.341ProAla: 3.341 ± 0.489
0.159ProCys: 0.159 ± 0.126
3.659ProAsp: 3.659 ± 0.461
5.171ProGlu: 5.171 ± 0.657
1.511ProPhe: 1.511 ± 0.409
4.375ProGly: 4.375 ± 0.623
0.875ProHis: 0.875 ± 0.318
2.148ProIle: 2.148 ± 0.325
2.386ProLys: 2.386 ± 0.476
2.386ProLeu: 2.386 ± 0.579
1.193ProMet: 1.193 ± 0.362
1.511ProAsn: 1.511 ± 0.317
1.193ProPro: 1.193 ± 0.382
1.83ProGln: 1.83 ± 0.444
1.034ProArg: 1.034 ± 0.298
2.068ProSer: 2.068 ± 0.429
2.148ProThr: 2.148 ± 0.578
3.977ProVal: 3.977 ± 0.48
0.239ProTrp: 0.239 ± 0.11
0.875ProTyr: 0.875 ± 0.238
0.0ProXaa: 0.0 ± 0.0
Gln
7.637GlnAla: 7.637 ± 0.868
0.159GlnCys: 0.159 ± 0.127
3.5GlnAsp: 3.5 ± 0.897
4.773GlnGlu: 4.773 ± 0.796
1.671GlnPhe: 1.671 ± 0.37
4.137GlnGly: 4.137 ± 0.535
0.557GlnHis: 0.557 ± 0.328
2.307GlnIle: 2.307 ± 0.384
3.023GlnLys: 3.023 ± 0.853
3.818GlnLeu: 3.818 ± 0.423
1.273GlnMet: 1.273 ± 0.323
1.989GlnAsn: 1.989 ± 0.518
1.909GlnPro: 1.909 ± 0.336
3.977GlnGln: 3.977 ± 0.677
3.023GlnArg: 3.023 ± 0.549
3.182GlnSer: 3.182 ± 0.673
2.625GlnThr: 2.625 ± 0.524
2.943GlnVal: 2.943 ± 0.539
0.398GlnTrp: 0.398 ± 0.132
1.034GlnTyr: 1.034 ± 0.394
0.0GlnXaa: 0.0 ± 0.0
Arg
3.182ArgAla: 3.182 ± 0.448
0.398ArgCys: 0.398 ± 0.261
3.58ArgAsp: 3.58 ± 0.875
4.296ArgGlu: 4.296 ± 0.843
1.989ArgPhe: 1.989 ± 0.43
2.784ArgGly: 2.784 ± 0.537
0.716ArgHis: 0.716 ± 0.321
3.818ArgIle: 3.818 ± 0.592
3.102ArgLys: 3.102 ± 0.452
4.852ArgLeu: 4.852 ± 0.801
1.909ArgMet: 1.909 ± 0.369
2.386ArgAsn: 2.386 ± 0.412
1.671ArgPro: 1.671 ± 0.343
3.341ArgGln: 3.341 ± 0.72
2.625ArgArg: 2.625 ± 0.519
3.421ArgSer: 3.421 ± 0.664
3.102ArgThr: 3.102 ± 0.369
4.216ArgVal: 4.216 ± 0.505
0.557ArgTrp: 0.557 ± 0.202
2.386ArgTyr: 2.386 ± 0.492
0.0ArgXaa: 0.0 ± 0.0
Ser
5.171SerAla: 5.171 ± 0.619
0.08SerCys: 0.08 ± 0.087
3.659SerAsp: 3.659 ± 0.45
4.057SerGlu: 4.057 ± 0.464
1.83SerPhe: 1.83 ± 0.449
4.137SerGly: 4.137 ± 0.614
0.716SerHis: 0.716 ± 0.242
3.5SerIle: 3.5 ± 0.526
4.455SerLys: 4.455 ± 0.683
4.057SerLeu: 4.057 ± 0.847
1.511SerMet: 1.511 ± 0.305
2.386SerAsn: 2.386 ± 0.457
2.148SerPro: 2.148 ± 0.366
2.784SerGln: 2.784 ± 0.507
2.546SerArg: 2.546 ± 0.509
2.546SerSer: 2.546 ± 0.83
3.341SerThr: 3.341 ± 0.68
3.341SerVal: 3.341 ± 0.546
0.636SerTrp: 0.636 ± 0.228
1.193SerTyr: 1.193 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
4.296ThrAla: 4.296 ± 0.735
0.477ThrCys: 0.477 ± 0.237
3.5ThrAsp: 3.5 ± 0.514
3.341ThrGlu: 3.341 ± 0.493
1.75ThrPhe: 1.75 ± 0.341
6.125ThrGly: 6.125 ± 0.926
0.795ThrHis: 0.795 ± 0.36
3.182ThrIle: 3.182 ± 0.756
3.5ThrLys: 3.5 ± 0.596
4.057ThrLeu: 4.057 ± 0.549
1.273ThrMet: 1.273 ± 0.235
2.148ThrAsn: 2.148 ± 0.614
3.102ThrPro: 3.102 ± 0.543
2.068ThrGln: 2.068 ± 0.5
3.421ThrArg: 3.421 ± 0.464
3.5ThrSer: 3.5 ± 0.552
2.546ThrThr: 2.546 ± 0.528
4.773ThrVal: 4.773 ± 1.157
0.795ThrTrp: 0.795 ± 0.367
1.432ThrTyr: 1.432 ± 0.29
0.0ThrXaa: 0.0 ± 0.0
Val
4.534ValAla: 4.534 ± 0.608
0.318ValCys: 0.318 ± 0.231
4.057ValAsp: 4.057 ± 0.628
5.012ValGlu: 5.012 ± 0.602
2.227ValPhe: 2.227 ± 0.397
3.739ValGly: 3.739 ± 0.492
1.114ValHis: 1.114 ± 0.435
3.898ValIle: 3.898 ± 0.497
5.171ValLys: 5.171 ± 0.716
4.137ValLeu: 4.137 ± 0.84
1.193ValMet: 1.193 ± 0.275
2.466ValAsn: 2.466 ± 0.538
3.898ValPro: 3.898 ± 0.788
2.307ValGln: 2.307 ± 0.398
3.261ValArg: 3.261 ± 0.632
3.341ValSer: 3.341 ± 0.458
4.375ValThr: 4.375 ± 0.762
4.375ValVal: 4.375 ± 0.511
0.875ValTrp: 0.875 ± 0.248
2.148ValTyr: 2.148 ± 0.668
0.0ValXaa: 0.0 ± 0.0
Trp
1.432TrpAla: 1.432 ± 0.374
0.318TrpCys: 0.318 ± 0.159
0.875TrpAsp: 0.875 ± 0.34
0.557TrpGlu: 0.557 ± 0.183
0.318TrpPhe: 0.318 ± 0.187
0.875TrpGly: 0.875 ± 0.211
0.398TrpHis: 0.398 ± 0.174
0.636TrpIle: 0.636 ± 0.265
1.273TrpLys: 1.273 ± 0.343
1.432TrpLeu: 1.432 ± 0.383
0.477TrpMet: 0.477 ± 0.226
0.477TrpAsn: 0.477 ± 0.187
0.875TrpPro: 0.875 ± 0.326
0.716TrpGln: 0.716 ± 0.283
0.875TrpArg: 0.875 ± 0.257
1.273TrpSer: 1.273 ± 0.406
0.398TrpThr: 0.398 ± 0.182
0.477TrpVal: 0.477 ± 0.24
0.239TrpTrp: 0.239 ± 0.097
0.636TrpTyr: 0.636 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.612
0.398TyrCys: 0.398 ± 0.204
2.386TyrAsp: 2.386 ± 0.516
1.432TyrGlu: 1.432 ± 0.332
1.193TyrPhe: 1.193 ± 0.316
2.227TyrGly: 2.227 ± 0.479
0.716TyrHis: 0.716 ± 0.325
1.352TyrIle: 1.352 ± 0.313
1.75TyrLys: 1.75 ± 0.362
2.227TyrLeu: 2.227 ± 0.311
1.273TyrMet: 1.273 ± 0.34
1.75TyrAsn: 1.75 ± 0.447
1.352TyrPro: 1.352 ± 0.265
1.671TyrGln: 1.671 ± 0.357
2.148TyrArg: 2.148 ± 0.38
1.671TyrSer: 1.671 ± 0.491
1.511TyrThr: 1.511 ± 0.376
1.352TyrVal: 1.352 ± 0.32
0.716TyrTrp: 0.716 ± 0.253
0.636TyrTyr: 0.636 ± 0.231
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 40 proteins (12572 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski