Red clover powdery mildew-associated totivirus 5
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3Q2D7|A0A0S3Q2D7_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 5 OX=1714366 GN=RdRp PE=3 SV=1
MM1 pKa = 8.01 EE2 pKa = 4.05 YY3 pKa = 10.11 TRR5 pKa = 11.84 GLFNVDD11 pKa = 3.03 GMIEE15 pKa = 4.2 SFGDD19 pKa = 3.17 GLIYY23 pKa = 10.61 HH24 pKa = 6.35 KK25 pKa = 9.75 TRR27 pKa = 11.84 LALNIADD34 pKa = 4.02 YY35 pKa = 11.25 NRR37 pKa = 11.84 ADD39 pKa = 4.21 FGTGDD44 pKa = 3.13 IQMNRR49 pKa = 11.84 HH50 pKa = 4.43 STVNVRR56 pKa = 11.84 SDD58 pKa = 3.34 RR59 pKa = 11.84 VSALAKK65 pKa = 10.1 LSEE68 pKa = 4.51 SNGEE72 pKa = 3.81 KK73 pKa = 10.42 FKK75 pKa = 10.91 FRR77 pKa = 11.84 RR78 pKa = 11.84 LTNCLSQSTVYY89 pKa = 10.74 GVVNTLRR96 pKa = 11.84 STTPQHH102 pKa = 6.86 SYY104 pKa = 11.25 LGMSKK109 pKa = 10.43 KK110 pKa = 10.63 YY111 pKa = 8.9 LTNEE115 pKa = 3.92 GLPNEE120 pKa = 4.09 QIIIKK125 pKa = 9.93 RR126 pKa = 11.84 LRR128 pKa = 11.84 EE129 pKa = 3.97 LGMHH133 pKa = 6.8 HH134 pKa = 6.95 DD135 pKa = 3.78 VKK137 pKa = 10.66 EE138 pKa = 3.87 ARR140 pKa = 11.84 FGQFYY145 pKa = 11.56 NMLFTKK151 pKa = 10.08 DD152 pKa = 3.75 YY153 pKa = 8.61 YY154 pKa = 11.55 DD155 pKa = 3.75 NMTALFAKK163 pKa = 10.22 LHH165 pKa = 5.84 LFSTRR170 pKa = 11.84 LSIQEE175 pKa = 3.66 RR176 pKa = 11.84 MGVHH180 pKa = 6.35 DD181 pKa = 4.85 HH182 pKa = 7.26 DD183 pKa = 4.75 VVISTASLTKK193 pKa = 9.97 MVGLANDD200 pKa = 4.49 DD201 pKa = 3.3 EE202 pKa = 4.51 RR203 pKa = 11.84 LAYY206 pKa = 9.9 FKK208 pKa = 11.08 SLLSSGTGRR217 pKa = 11.84 LGFYY221 pKa = 9.56 EE222 pKa = 3.75 QDD224 pKa = 3.7 YY225 pKa = 10.95 NLEE228 pKa = 4.11 TMLQNSIAWFNVNKK242 pKa = 9.99 NDD244 pKa = 3.83 PAARR248 pKa = 11.84 EE249 pKa = 3.72 YY250 pKa = 10.82 RR251 pKa = 11.84 QTVKK255 pKa = 9.74 VWHH258 pKa = 6.41 MYY260 pKa = 10.33 KK261 pKa = 10.26 YY262 pKa = 10.85 DD263 pKa = 4.27 DD264 pKa = 3.63 GHH266 pKa = 5.97 NVSGRR271 pKa = 11.84 SFGSEE276 pKa = 3.67 FGFDD280 pKa = 3.06 GGKK283 pKa = 10.0 FLIPEE288 pKa = 4.13 CHH290 pKa = 5.22 NTINYY295 pKa = 8.89 IDD297 pKa = 3.93 GVKK300 pKa = 10.0 LVKK303 pKa = 10.73 SRR305 pKa = 11.84 DD306 pKa = 3.02 NWFSDD311 pKa = 3.38 EE312 pKa = 4.78 NIDD315 pKa = 4.79 KK316 pKa = 10.79 FGSRR320 pKa = 11.84 YY321 pKa = 10.12 AGYY324 pKa = 10.77 LNLSGMTEE332 pKa = 3.95 LEE334 pKa = 3.86 IAIVNHH340 pKa = 6.3 ILLNTDD346 pKa = 2.39 RR347 pKa = 11.84 MTPFLCDD354 pKa = 3.26 QEE356 pKa = 5.39 LALCSDD362 pKa = 3.46 SDD364 pKa = 3.78 KK365 pKa = 11.21 FLALASGEE373 pKa = 4.01 ISTKK377 pKa = 7.64 QTVVSSKK384 pKa = 7.27 QVRR387 pKa = 11.84 TVMSKK392 pKa = 10.59 LAMNHH397 pKa = 6.16 RR398 pKa = 11.84 AQEE401 pKa = 4.11 DD402 pKa = 3.81 MLCAVRR408 pKa = 11.84 SCKK411 pKa = 10.31 YY412 pKa = 10.74 LLAQPANEE420 pKa = 4.19 TAEE423 pKa = 4.31 SHH425 pKa = 4.84 WWNVLPKK432 pKa = 10.16 IVDD435 pKa = 3.99 FPKK438 pKa = 10.2 MGLKK442 pKa = 9.97 RR443 pKa = 11.84 AALHH447 pKa = 6.91 FMLEE451 pKa = 4.23 DD452 pKa = 3.3 EE453 pKa = 4.96 GVCISVDD460 pKa = 3.7 SIKK463 pKa = 10.67 YY464 pKa = 8.8 ARR466 pKa = 11.84 EE467 pKa = 3.79 YY468 pKa = 10.99 LSKK471 pKa = 10.5 CDD473 pKa = 3.57 RR474 pKa = 11.84 AILEE478 pKa = 4.48 SVFVNSCWFWAEE490 pKa = 3.57 YY491 pKa = 8.73 LTIYY495 pKa = 10.82 NKK497 pKa = 9.87 TNSYY501 pKa = 11.16 DD502 pKa = 3.38 LAAGLLRR509 pKa = 11.84 GNDD512 pKa = 2.82 SWLVEE517 pKa = 4.09 RR518 pKa = 11.84 EE519 pKa = 3.99 RR520 pKa = 11.84 PDD522 pKa = 3.03 IMVSALIGRR531 pKa = 11.84 PVPIAVHH538 pKa = 5.65 SCCTTQWAKK547 pKa = 10.4 PLSEE551 pKa = 4.04 LARR554 pKa = 11.84 LKK556 pKa = 10.92 VCFGTISLTGRR567 pKa = 11.84 DD568 pKa = 4.01 DD569 pKa = 3.99 YY570 pKa = 11.88 NYY572 pKa = 9.78 TLSDD576 pKa = 3.27 KK577 pKa = 11.11 DD578 pKa = 3.87 VIFNSVVPPSGLALVTGLGGSLIQGTPYY606 pKa = 11.21 GSMFGINQAVKK617 pKa = 9.5 VRR619 pKa = 11.84 KK620 pKa = 9.56 GMRR623 pKa = 11.84 TITSLNYY630 pKa = 9.87 NDD632 pKa = 4.27 LWALGVVSRR641 pKa = 11.84 FNGYY645 pKa = 7.88 DD646 pKa = 3.45 LKK648 pKa = 11.1 YY649 pKa = 10.23 KK650 pKa = 10.53 CPSSEE655 pKa = 4.11 NVHH658 pKa = 6.54 TIYY661 pKa = 10.82 AANNVSIANPPVLFDD676 pKa = 4.44 PEE678 pKa = 4.2 ASNLSYY684 pKa = 10.72 AIRR687 pKa = 11.84 SITEE691 pKa = 3.39 RR692 pKa = 11.84 DD693 pKa = 3.2 IVFGSDD699 pKa = 2.71 TTTLLSSKK707 pKa = 10.68 SVFYY711 pKa = 10.59 WNRR714 pKa = 11.84 FEE716 pKa = 4.4 STTLEE721 pKa = 4.13 SPKK724 pKa = 9.61 WSAGIISNDD733 pKa = 3.03 VEE735 pKa = 4.23 EE736 pKa = 4.58 AVLIRR741 pKa = 11.84 GFEE744 pKa = 4.26 CYY746 pKa = 10.36 LDD748 pKa = 3.58 EE749 pKa = 4.45 TKK751 pKa = 10.54 KK752 pKa = 9.27 YY753 pKa = 8.19 TVAISAEE760 pKa = 4.06 YY761 pKa = 10.19 DD762 pKa = 3.59 VEE764 pKa = 4.47 VSDD767 pKa = 4.05 FHH769 pKa = 7.0 VAKK772 pKa = 9.14 LTAGIGLPTLDD783 pKa = 5.05 GGLLLDD789 pKa = 4.58 AEE791 pKa = 4.69 NPAEE795 pKa = 4.14 QTTEE799 pKa = 3.49 LDD801 pKa = 3.62 MPVLEE806 pKa = 5.46 DD807 pKa = 3.4 SALGAGSS814 pKa = 3.25
Molecular weight: 91.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.499
IPC2_protein 5.512
IPC_protein 5.55
Toseland 5.842
ProMoST 5.842
Dawson 5.728
Bjellqvist 5.779
Wikipedia 5.715
Rodwell 5.703
Grimsley 5.944
Solomon 5.728
Lehninger 5.715
Nozaki 5.97
DTASelect 6.148
Thurlkill 6.097
EMBOSS 6.059
Sillero 6.059
Patrickios 3.923
IPC_peptide 5.753
IPC2_peptide 6.059
IPC2.peptide.svr19 6.049
Protein with the highest isoelectric point:
>tr|A0A0S3Q2D7|A0A0S3Q2D7_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 5 OX=1714366 GN=RdRp PE=3 SV=1
MM1 pKa = 7.81 PYY3 pKa = 10.36 VPTFLAPTDD12 pKa = 3.45 SSRR15 pKa = 11.84 VFRR18 pKa = 11.84 EE19 pKa = 3.36 VDD21 pKa = 3.86 FNEE24 pKa = 3.99 ADD26 pKa = 3.56 LVLFEE31 pKa = 4.96 CLSGLEE37 pKa = 4.71 LDD39 pKa = 4.77 GSMHH43 pKa = 6.96 LHH45 pKa = 5.45 IQEE48 pKa = 4.13 SAVPIIAVYY57 pKa = 9.82 LPKK60 pKa = 10.83 VKK62 pKa = 9.28 VTACYY67 pKa = 10.25 ISIDD71 pKa = 3.77 YY72 pKa = 9.9 QIKK75 pKa = 8.36 HH76 pKa = 5.27 TNKK79 pKa = 10.16 DD80 pKa = 3.08 VLLRR84 pKa = 11.84 FSRR87 pKa = 11.84 MQYY90 pKa = 10.96 GPDD93 pKa = 3.66 LFPFGLVSDD102 pKa = 4.65 LDD104 pKa = 3.46 IVRR107 pKa = 11.84 YY108 pKa = 10.13 AFFISRR114 pKa = 11.84 KK115 pKa = 6.91 TIKK118 pKa = 9.83 KK119 pKa = 10.01 RR120 pKa = 11.84 KK121 pKa = 8.96 DD122 pKa = 3.3 YY123 pKa = 10.45 KK124 pKa = 10.86 IKK126 pKa = 10.49 DD127 pKa = 3.62 FPIIDD132 pKa = 3.24 SWFRR136 pKa = 11.84 GSTEE140 pKa = 3.87 PPVSKK145 pKa = 10.84 VSVNHH150 pKa = 5.79 LRR152 pKa = 11.84 HH153 pKa = 4.7 MTMAEE158 pKa = 3.78 VRR160 pKa = 11.84 LMGRR164 pKa = 11.84 GVIEE168 pKa = 4.86 DD169 pKa = 3.54 KK170 pKa = 11.07 VSYY173 pKa = 10.29 ILPFLSRR180 pKa = 11.84 MAKK183 pKa = 10.0 VGMSEE188 pKa = 3.94 AMFIGIVLWANSLPTQQHH206 pKa = 5.58 EE207 pKa = 4.29 LCKK210 pKa = 10.82 LSGLWDD216 pKa = 3.9 LSFTSMEE223 pKa = 4.16 DD224 pKa = 3.03 FATKK228 pKa = 10.24 VKK230 pKa = 10.81 ARR232 pKa = 11.84 FSLRR236 pKa = 11.84 LKK238 pKa = 10.48 ALQNLVSVDD247 pKa = 3.22 LHH249 pKa = 6.71 CFFEE253 pKa = 5.57 LEE255 pKa = 4.2 VLVNRR260 pKa = 11.84 GVGEE264 pKa = 4.23 LNWEE268 pKa = 4.03 KK269 pKa = 11.16 EE270 pKa = 3.95 KK271 pKa = 10.86 MNRR274 pKa = 11.84 TRR276 pKa = 11.84 PNLAQFKK283 pKa = 9.32 PEE285 pKa = 3.89 QVFSKK290 pKa = 10.53 ACSLFTDD297 pKa = 3.77 ILRR300 pKa = 11.84 RR301 pKa = 11.84 GGRR304 pKa = 11.84 PKK306 pKa = 10.2 KK307 pKa = 10.52 RR308 pKa = 11.84 NWSEE312 pKa = 3.53 FWSNRR317 pKa = 11.84 WEE319 pKa = 3.98 WAPTGSSHH327 pKa = 6.18 SQYY330 pKa = 11.54 AEE332 pKa = 3.9 DD333 pKa = 5.31 DD334 pKa = 4.1 VYY336 pKa = 11.44 KK337 pKa = 11.07 SKK339 pKa = 11.49 DD340 pKa = 3.05 PFNRR344 pKa = 11.84 HH345 pKa = 5.63 KK346 pKa = 11.06 LFTLCSMPEE355 pKa = 3.47 RR356 pKa = 11.84 SFEE359 pKa = 4.1 YY360 pKa = 9.93 FLKK363 pKa = 10.63 RR364 pKa = 11.84 KK365 pKa = 9.47 PEE367 pKa = 4.33 TIAWTSTKK375 pKa = 10.56 YY376 pKa = 10.29 EE377 pKa = 4.04 WTKK380 pKa = 9.96 MRR382 pKa = 11.84 AIYY385 pKa = 10.14 GVDD388 pKa = 2.52 ITNFIMTTFAMGDD401 pKa = 3.84 CEE403 pKa = 5.59 DD404 pKa = 3.88 VLSSKK409 pKa = 10.51 FPIGKK414 pKa = 9.2 AANEE418 pKa = 4.11 VNVRR422 pKa = 11.84 STVKK426 pKa = 9.39 TLLGNGVPFCFDD438 pKa = 3.37 FEE440 pKa = 5.02 DD441 pKa = 4.71 FNSQHH446 pKa = 6.7 SIEE449 pKa = 5.01 SMVQVIRR456 pKa = 11.84 AYY458 pKa = 10.32 SSTFKK463 pKa = 10.07 MHH465 pKa = 6.86 MSEE468 pKa = 3.98 QQYY471 pKa = 10.66 NAIDD475 pKa = 3.5 WVCRR479 pKa = 11.84 SFYY482 pKa = 11.04 DD483 pKa = 3.83 SFVVEE488 pKa = 5.0 DD489 pKa = 3.57 NRR491 pKa = 11.84 GDD493 pKa = 3.79 KK494 pKa = 11.01 DD495 pKa = 3.47 KK496 pKa = 11.52 GPCNSSASSRR506 pKa = 11.84 EE507 pKa = 3.5 RR508 pKa = 11.84 AKK510 pKa = 10.11 MYY512 pKa = 9.74 RR513 pKa = 11.84 TMGTLLSGCRR523 pKa = 11.84 LTTFLNTVLNYY534 pKa = 9.92 IYY536 pKa = 11.09 VNLSIEE542 pKa = 4.26 GDD544 pKa = 3.82 DD545 pKa = 6.35 LIATHH550 pKa = 6.59 NGDD553 pKa = 4.92 DD554 pKa = 3.38 ILAVVSSYY562 pKa = 11.45 RR563 pKa = 11.84 EE564 pKa = 3.63 VQAISRR570 pKa = 11.84 GAEE573 pKa = 3.38 RR574 pKa = 11.84 HH575 pKa = 5.2 QIRR578 pKa = 11.84 FQPSKK583 pKa = 10.88 CFLGSVAEE591 pKa = 4.17 FLRR594 pKa = 11.84 IDD596 pKa = 3.48 HH597 pKa = 6.79 HH598 pKa = 7.95 SGAGGQYY605 pKa = 8.12 LTRR608 pKa = 11.84 AISTLVHH615 pKa = 6.77 GPTEE619 pKa = 3.89 MAVPNNLVAQLTAINTRR636 pKa = 11.84 ANEE639 pKa = 3.89 AKK641 pKa = 10.64 ARR643 pKa = 11.84 GADD646 pKa = 3.23 HH647 pKa = 7.03 AFIAGVVKK655 pKa = 10.67 SQLPYY660 pKa = 10.42 LCKK663 pKa = 9.99 VWQITEE669 pKa = 4.32 DD670 pKa = 3.42 EE671 pKa = 4.49 VEE673 pKa = 4.48 AVFSTHH679 pKa = 6.21 LCFGGLSNEE688 pKa = 4.04 ITPKK692 pKa = 10.49 SLSKK696 pKa = 10.83 SIILEE701 pKa = 3.83 EE702 pKa = 5.74 DD703 pKa = 2.42 ITANKK708 pKa = 9.37 RR709 pKa = 11.84 PSVLADD715 pKa = 3.21 EE716 pKa = 5.73 GRR718 pKa = 11.84 FFPGAFSYY726 pKa = 10.87 AKK728 pKa = 10.04 SLTSTIIPEE737 pKa = 4.21 SYY739 pKa = 9.77 FGKK742 pKa = 10.29 VEE744 pKa = 4.04 HH745 pKa = 6.39 AVSRR749 pKa = 11.84 TVTALSCSRR758 pKa = 11.84 RR759 pKa = 11.84 FRR761 pKa = 11.84 LVVKK765 pKa = 10.24 DD766 pKa = 3.62 NKK768 pKa = 10.14 TPGVTDD774 pKa = 3.59 YY775 pKa = 11.23 IKK777 pKa = 10.9 ANRR780 pKa = 11.84 YY781 pKa = 9.17 GLFRR785 pKa = 11.84 SKK787 pKa = 9.69 LTAGKK792 pKa = 8.27 ITLARR797 pKa = 11.84 AFNIPIIDD805 pKa = 3.3 IKK807 pKa = 9.71 YY808 pKa = 8.46 QKK810 pKa = 10.34 QEE812 pKa = 3.69 ILEE815 pKa = 4.21 ILASEE820 pKa = 4.05 QDD822 pKa = 3.12 KK823 pKa = 10.93 MEE825 pKa = 4.56 ALRR828 pKa = 11.84 ILFF831 pKa = 4.11
Molecular weight: 94.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.772
IPC2_protein 8.024
IPC_protein 7.849
Toseland 7.732
ProMoST 8.434
Dawson 8.668
Bjellqvist 8.873
Wikipedia 8.653
Rodwell 8.741
Grimsley 7.761
Solomon 8.726
Lehninger 8.726
Nozaki 9.048
DTASelect 8.653
Thurlkill 8.741
EMBOSS 8.858
Sillero 9.004
Patrickios 4.546
IPC_peptide 8.726
IPC2_peptide 7.658
IPC2.peptide.svr19 7.868
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1645
814
831
822.5
92.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.626 ± 0.006
1.763 ± 0.034
5.714 ± 0.439
5.836 ± 0.147
5.228 ± 0.739
5.836 ± 0.635
2.249 ± 0.03
5.593 ± 0.443
5.836 ± 0.538
9.422 ± 0.714
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.675 ± 0.022
4.863 ± 0.725
3.343 ± 0.412
2.492 ± 0.224
5.653 ± 0.491
8.754 ± 0.025
5.897 ± 0.196
6.93 ± 0.138
1.459 ± 0.012
3.83 ± 0.472
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here