Desulfofundulus salinum
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2755 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A494X0T6|A0A494X0T6_9FIRM NAD(P)/FAD-dependent oxidoreductase OS=Desulfofundulus salinum OX=2419843 GN=D7024_05920 PE=4 SV=1
MM1 pKa = 7.98 EE2 pKa = 5.39 DD3 pKa = 3.13 LKK5 pKa = 11.61 SRR7 pKa = 11.84 VAYY10 pKa = 10.13 LQGLSAGMDD19 pKa = 3.41 LEE21 pKa = 4.71 KK22 pKa = 10.64 EE23 pKa = 4.39 SKK25 pKa = 9.99 EE26 pKa = 3.8 GRR28 pKa = 11.84 LLSSIIDD35 pKa = 3.77 VLGDD39 pKa = 3.41 FADD42 pKa = 4.17 SFNEE46 pKa = 3.88 LQEE49 pKa = 4.3 AQGQLEE55 pKa = 5.02 DD56 pKa = 3.82 YY57 pKa = 10.78 LEE59 pKa = 5.22 SIDD62 pKa = 3.94 EE63 pKa = 4.35 DD64 pKa = 4.41 LYY66 pKa = 11.36 SLEE69 pKa = 4.66 DD70 pKa = 3.91 EE71 pKa = 5.11 IYY73 pKa = 10.45 DD74 pKa = 3.82 GRR76 pKa = 11.84 PGMEE80 pKa = 4.66 DD81 pKa = 3.29 EE82 pKa = 5.9 DD83 pKa = 4.57 YY84 pKa = 11.3 MEE86 pKa = 4.68 VEE88 pKa = 4.88 CPRR91 pKa = 11.84 CGEE94 pKa = 4.05 IVCFDD99 pKa = 3.93 RR100 pKa = 11.84 DD101 pKa = 3.56 IVDD104 pKa = 4.26 DD105 pKa = 4.19 EE106 pKa = 4.72 DD107 pKa = 4.58 VIEE110 pKa = 4.5 VTCPNCDD117 pKa = 2.5 EE118 pKa = 4.37 VVFVNDD124 pKa = 3.65 DD125 pKa = 3.74 TYY127 pKa = 12.06 ASADD131 pKa = 3.7 EE132 pKa = 4.57 PEE134 pKa = 4.46 LLEE137 pKa = 4.67 GRR139 pKa = 11.84 TQVEE143 pKa = 4.44 HH144 pKa = 7.29 EE145 pKa = 4.79 DD146 pKa = 3.83 DD147 pKa = 3.69 LL148 pKa = 6.52
Molecular weight: 16.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.541
IPC_protein 3.541
Toseland 3.325
ProMoST 3.694
Dawson 3.528
Bjellqvist 3.681
Wikipedia 3.452
Rodwell 3.376
Grimsley 3.236
Solomon 3.516
Lehninger 3.465
Nozaki 3.643
DTASelect 3.846
Thurlkill 3.376
EMBOSS 3.465
Sillero 3.656
Patrickios 1.024
IPC_peptide 3.516
IPC2_peptide 3.643
IPC2.peptide.svr19 3.668
Protein with the highest isoelectric point:
>tr|A0A494WRU4|A0A494WRU4_9FIRM ParB/RepB/Spo0J family partition protein OS=Desulfofundulus salinum OX=2419843 GN=D7024_00090 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 9.68 QPKK8 pKa = 8.39 NRR10 pKa = 11.84 KK11 pKa = 8.29 RR12 pKa = 11.84 KK13 pKa = 9.45 KK14 pKa = 8.77 IHH16 pKa = 6.15 GFLKK20 pKa = 10.53 RR21 pKa = 11.84 MSTRR25 pKa = 11.84 AGRR28 pKa = 11.84 NVIKK32 pKa = 10.49 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.22 GRR39 pKa = 11.84 KK40 pKa = 9.04 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.435
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.457
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.179
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2755
0
2755
821128
22
1847
298.1
32.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.472 ± 0.062
1.252 ± 0.025
4.532 ± 0.034
7.08 ± 0.051
3.566 ± 0.029
8.437 ± 0.041
1.888 ± 0.021
5.85 ± 0.045
4.609 ± 0.042
10.793 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.426 ± 0.021
2.962 ± 0.025
4.913 ± 0.03
3.392 ± 0.033
7.0 ± 0.047
4.558 ± 0.032
4.859 ± 0.033
8.438 ± 0.043
1.039 ± 0.019
2.932 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here