Cellulomonas sp. PhB150

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3418 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N2FI34|A0A3N2FI34_9CELL Enamine deaminase RidA (YjgF/YER057c/UK114 family) OS=Cellulomonas sp. PhB150 OX=2485188 GN=EDF34_3059 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84ALLVLVAASGLALAGCSSAPEE23 pKa = 4.29VVTSYY28 pKa = 11.73ADD30 pKa = 3.45YY31 pKa = 10.03PSYY34 pKa = 8.56EE35 pKa = 4.21TPQDD39 pKa = 3.18IVDD42 pKa = 3.81AADD45 pKa = 3.25VVVRR49 pKa = 11.84GEE51 pKa = 4.02VTGARR56 pKa = 11.84VKK58 pKa = 10.41EE59 pKa = 4.0DD60 pKa = 3.33QPIVSTTGDD69 pKa = 3.39PSLNPQAGLDD79 pKa = 3.74EE80 pKa = 4.78GTDD83 pKa = 3.55VPGVVVTISTVRR95 pKa = 11.84VEE97 pKa = 4.16EE98 pKa = 4.36VIKK101 pKa = 11.02GDD103 pKa = 3.66VAVGDD108 pKa = 4.28VIEE111 pKa = 4.2VAQLGGLYY119 pKa = 10.68DD120 pKa = 3.52GVRR123 pKa = 11.84YY124 pKa = 10.11VEE126 pKa = 4.32ASTVTLEE133 pKa = 4.52KK134 pKa = 11.04GSDD137 pKa = 3.49DD138 pKa = 4.3YY139 pKa = 11.67MLLLADD145 pKa = 4.96HH146 pKa = 6.97GPGVPYY152 pKa = 10.79DD153 pKa = 3.91LLHH156 pKa = 6.76PVQAMYY162 pKa = 9.79TVDD165 pKa = 5.06SGDD168 pKa = 4.26LDD170 pKa = 3.72PVADD174 pKa = 3.93NDD176 pKa = 4.03VAVDD180 pKa = 3.89TVDD183 pKa = 5.31DD184 pKa = 4.28LAEE187 pKa = 4.23LAQDD191 pKa = 3.44

Molecular weight: 19.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N2G2V1|A0A3N2G2V1_9CELL Cysteine dioxygenase type I OS=Cellulomonas sp. PhB150 OX=2485188 GN=EDF34_0423 PE=3 SV=1
MM1 pKa = 7.08GRR3 pKa = 11.84AATVLVRR10 pKa = 11.84ATVPPMGRR18 pKa = 11.84AATVPARR25 pKa = 11.84ATVLPTATVSVMTAVAARR43 pKa = 11.84RR44 pKa = 11.84TGRR47 pKa = 11.84AATGHH52 pKa = 5.61GRR54 pKa = 11.84GIARR58 pKa = 11.84ATATVPAPVTVPLRR72 pKa = 11.84ADD74 pKa = 3.25VRR76 pKa = 11.84PTARR80 pKa = 11.84AVTVRR85 pKa = 11.84GPATVLPTVTVRR97 pKa = 11.84VTTAVAVRR105 pKa = 11.84PMARR109 pKa = 11.84AATARR114 pKa = 11.84ATTAVAVPRR123 pKa = 11.84TGRR126 pKa = 11.84ATARR130 pKa = 11.84AATGRR135 pKa = 11.84RR136 pKa = 11.84TATVPRR142 pKa = 11.84RR143 pKa = 11.84ATALARR149 pKa = 11.84AIVRR153 pKa = 11.84RR154 pKa = 11.84RR155 pKa = 11.84ATALARR161 pKa = 11.84AIVRR165 pKa = 11.84PTATVRR171 pKa = 11.84VTTVAAVPRR180 pKa = 11.84TGRR183 pKa = 11.84AATALARR190 pKa = 11.84VTVLPTAIDD199 pKa = 3.6RR200 pKa = 11.84ATTAVAARR208 pKa = 11.84PLGRR212 pKa = 11.84AATALVATARR222 pKa = 11.84PTATAPRR229 pKa = 11.84APRR232 pKa = 11.84RR233 pKa = 11.84ASVVTAPRR241 pKa = 11.84TATAVAVRR249 pKa = 11.84PTGRR253 pKa = 11.84ALIALGAAIVLPTVTARR270 pKa = 11.84AATGRR275 pKa = 11.84HH276 pKa = 3.9RR277 pKa = 11.84VAVRR281 pKa = 11.84RR282 pKa = 11.84TAIARR287 pKa = 11.84HH288 pKa = 4.64TAIARR293 pKa = 11.84HH294 pKa = 4.59TAIVRR299 pKa = 11.84RR300 pKa = 11.84PATARR305 pKa = 11.84HH306 pKa = 5.93APHH309 pKa = 7.09PVTVRR314 pKa = 11.84ARR316 pKa = 11.84TVRR319 pKa = 11.84PRR321 pKa = 11.84VTVRR325 pKa = 11.84RR326 pKa = 11.84TATARR331 pKa = 11.84ARR333 pKa = 11.84GTGARR338 pKa = 11.84SPASGRR344 pKa = 11.84ATVRR348 pKa = 11.84APRR351 pKa = 11.84ARR353 pKa = 11.84PPAASAPLGRR363 pKa = 11.84RR364 pKa = 11.84TARR367 pKa = 11.84LAAIVPRR374 pKa = 11.84TALVPRR380 pKa = 11.84RR381 pKa = 11.84AIDD384 pKa = 3.66PRR386 pKa = 11.84PVTAARR392 pKa = 11.84RR393 pKa = 11.84ATGMTGPTPVVATGRR408 pKa = 11.84IARR411 pKa = 11.84RR412 pKa = 11.84ARR414 pKa = 11.84TRR416 pKa = 11.84AAVRR420 pKa = 11.84ATVRR424 pKa = 11.84RR425 pKa = 11.84RR426 pKa = 11.84TDD428 pKa = 3.0RR429 pKa = 11.84SATAVRR435 pKa = 11.84PAATTTASPVRR446 pKa = 11.84RR447 pKa = 11.84RR448 pKa = 11.84TSGRR452 pKa = 11.84PRR454 pKa = 11.84RR455 pKa = 3.86

Molecular weight: 47.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3418

0

3418

1165880

29

3045

341.1

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.25 ± 0.065

0.535 ± 0.009

6.519 ± 0.041

5.007 ± 0.038

2.589 ± 0.024

9.232 ± 0.04

1.993 ± 0.022

3.321 ± 0.03

1.841 ± 0.036

10.071 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.544 ± 0.016

1.628 ± 0.028

5.728 ± 0.033

2.662 ± 0.021

7.204 ± 0.052

5.53 ± 0.04

6.759 ± 0.059

10.133 ± 0.041

1.58 ± 0.019

1.875 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski