Amino acid dipepetide frequency for Cellulomonas sp. PhB150

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.501AlaAla: 22.501 ± 0.207
0.799AlaCys: 0.799 ± 0.029
9.284AlaAsp: 9.284 ± 0.115
7.117AlaGlu: 7.117 ± 0.1
3.522AlaPhe: 3.522 ± 0.058
13.347AlaGly: 13.347 ± 0.127
2.611AlaHis: 2.611 ± 0.05
4.685AlaIle: 4.685 ± 0.062
2.634AlaLys: 2.634 ± 0.068
14.674AlaLeu: 14.674 ± 0.154
2.577AlaMet: 2.577 ± 0.052
2.063AlaAsn: 2.063 ± 0.053
7.476AlaPro: 7.476 ± 0.104
3.972AlaGln: 3.972 ± 0.055
10.193AlaArg: 10.193 ± 0.141
7.691AlaSer: 7.691 ± 0.098
9.257AlaThr: 9.257 ± 0.136
13.382AlaVal: 13.382 ± 0.124
2.298AlaTrp: 2.298 ± 0.045
2.413AlaTyr: 2.413 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.698CysAla: 0.698 ± 0.023
0.051CysCys: 0.051 ± 0.007
0.333CysAsp: 0.333 ± 0.019
0.266CysGlu: 0.266 ± 0.016
0.153CysPhe: 0.153 ± 0.012
0.6CysGly: 0.6 ± 0.024
0.103CysHis: 0.103 ± 0.009
0.152CysIle: 0.152 ± 0.012
0.064CysLys: 0.064 ± 0.007
0.491CysLeu: 0.491 ± 0.019
0.074CysMet: 0.074 ± 0.008
0.107CysAsn: 0.107 ± 0.011
0.329CysPro: 0.329 ± 0.017
0.116CysGln: 0.116 ± 0.01
0.348CysArg: 0.348 ± 0.02
0.371CysSer: 0.371 ± 0.018
0.426CysThr: 0.426 ± 0.025
0.476CysVal: 0.476 ± 0.017
0.088CysTrp: 0.088 ± 0.008
0.105CysTyr: 0.105 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.257AspAla: 9.257 ± 0.082
0.277AspCys: 0.277 ± 0.015
5.106AspAsp: 5.106 ± 0.078
4.959AspGlu: 4.959 ± 0.074
1.365AspPhe: 1.365 ± 0.043
7.067AspGly: 7.067 ± 0.092
1.25AspHis: 1.25 ± 0.033
1.662AspIle: 1.662 ± 0.04
1.008AspLys: 1.008 ± 0.029
6.974AspLeu: 6.974 ± 0.083
0.683AspMet: 0.683 ± 0.025
0.848AspAsn: 0.848 ± 0.029
4.591AspPro: 4.591 ± 0.074
1.673AspGln: 1.673 ± 0.039
4.228AspArg: 4.228 ± 0.06
2.463AspSer: 2.463 ± 0.051
2.859AspThr: 2.859 ± 0.056
6.906AspVal: 6.906 ± 0.087
0.937AspTrp: 0.937 ± 0.031
1.071AspTyr: 1.071 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
6.642GluAla: 6.642 ± 0.089
0.234GluCys: 0.234 ± 0.016
2.598GluAsp: 2.598 ± 0.053
2.125GluGlu: 2.125 ± 0.054
1.104GluPhe: 1.104 ± 0.032
3.695GluGly: 3.695 ± 0.061
1.599GluHis: 1.599 ± 0.042
2.187GluIle: 2.187 ± 0.047
1.073GluLys: 1.073 ± 0.041
5.923GluLeu: 5.923 ± 0.078
0.758GluMet: 0.758 ± 0.032
0.852GluAsn: 0.852 ± 0.03
3.256GluPro: 3.256 ± 0.061
2.109GluGln: 2.109 ± 0.047
4.574GluArg: 4.574 ± 0.09
2.335GluSer: 2.335 ± 0.047
2.514GluThr: 2.514 ± 0.043
4.946GluVal: 4.946 ± 0.074
0.653GluTrp: 0.653 ± 0.029
0.889GluTyr: 0.889 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.561PheAla: 3.561 ± 0.063
0.17PheCys: 0.17 ± 0.014
2.011PheAsp: 2.011 ± 0.045
1.342PheGlu: 1.342 ± 0.037
0.803PhePhe: 0.803 ± 0.028
2.836PheGly: 2.836 ± 0.054
0.472PheHis: 0.472 ± 0.019
0.697PheIle: 0.697 ± 0.026
0.435PheLys: 0.435 ± 0.019
2.342PheLeu: 2.342 ± 0.053
0.335PheMet: 0.335 ± 0.018
0.467PheAsn: 0.467 ± 0.022
1.147PhePro: 1.147 ± 0.036
0.612PheGln: 0.612 ± 0.023
1.503PheArg: 1.503 ± 0.038
1.485PheSer: 1.485 ± 0.04
1.937PheThr: 1.937 ± 0.055
2.754PheVal: 2.754 ± 0.052
0.437PheTrp: 0.437 ± 0.018
0.546PheTyr: 0.546 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
11.801GlyAla: 11.801 ± 0.126
0.582GlyCys: 0.582 ± 0.023
5.413GlyAsp: 5.413 ± 0.077
4.398GlyGlu: 4.398 ± 0.073
2.807GlyPhe: 2.807 ± 0.05
7.99GlyGly: 7.99 ± 0.114
1.984GlyHis: 1.984 ± 0.041
3.679GlyIle: 3.679 ± 0.06
2.196GlyLys: 2.196 ± 0.054
9.313GlyLeu: 9.313 ± 0.103
1.741GlyMet: 1.741 ± 0.037
1.534GlyAsn: 1.534 ± 0.049
4.638GlyPro: 4.638 ± 0.069
2.497GlyGln: 2.497 ± 0.046
6.704GlyArg: 6.704 ± 0.088
5.869GlySer: 5.869 ± 0.091
6.782GlyThr: 6.782 ± 0.116
8.406GlyVal: 8.406 ± 0.092
1.811GlyTrp: 1.811 ± 0.036
2.176GlyTyr: 2.176 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.639HisAla: 2.639 ± 0.055
0.099HisCys: 0.099 ± 0.008
1.395HisAsp: 1.395 ± 0.038
1.126HisGlu: 1.126 ± 0.035
0.427HisPhe: 0.427 ± 0.02
2.006HisGly: 2.006 ± 0.048
0.566HisHis: 0.566 ± 0.022
0.503HisIle: 0.503 ± 0.022
0.284HisLys: 0.284 ± 0.015
2.207HisLeu: 2.207 ± 0.045
0.236HisMet: 0.236 ± 0.015
0.298HisAsn: 0.298 ± 0.017
1.389HisPro: 1.389 ± 0.038
0.533HisGln: 0.533 ± 0.024
1.577HisArg: 1.577 ± 0.039
0.779HisSer: 0.779 ± 0.029
1.068HisThr: 1.068 ± 0.033
2.09HisVal: 2.09 ± 0.048
0.31HisTrp: 0.31 ± 0.015
0.395HisTyr: 0.395 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
5.245IleAla: 5.245 ± 0.079
0.192IleCys: 0.192 ± 0.013
2.674IleAsp: 2.674 ± 0.048
2.02IleGlu: 2.02 ± 0.049
0.771IlePhe: 0.771 ± 0.025
3.667IleGly: 3.667 ± 0.064
0.549IleHis: 0.549 ± 0.022
1.022IleIle: 1.022 ± 0.032
0.692IleLys: 0.692 ± 0.028
2.531IleLeu: 2.531 ± 0.051
0.43IleMet: 0.43 ± 0.021
0.699IleAsn: 0.699 ± 0.029
1.772IlePro: 1.772 ± 0.041
0.708IleGln: 0.708 ± 0.026
1.925IleArg: 1.925 ± 0.044
1.75IleSer: 1.75 ± 0.039
2.318IleThr: 2.318 ± 0.051
3.29IleVal: 3.29 ± 0.056
0.39IleTrp: 0.39 ± 0.017
0.568IleTyr: 0.568 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
2.605LysAla: 2.605 ± 0.066
0.081LysCys: 0.081 ± 0.008
1.16LysAsp: 1.16 ± 0.042
0.729LysGlu: 0.729 ± 0.029
0.416LysPhe: 0.416 ± 0.021
1.483LysGly: 1.483 ± 0.047
0.394LysHis: 0.394 ± 0.017
0.768LysIle: 0.768 ± 0.03
0.729LysLys: 0.729 ± 0.034
1.616LysLeu: 1.616 ± 0.044
0.279LysMet: 0.279 ± 0.017
0.423LysAsn: 0.423 ± 0.019
1.17LysPro: 1.17 ± 0.04
0.587LysGln: 0.587 ± 0.025
1.152LysArg: 1.152 ± 0.031
1.034LysSer: 1.034 ± 0.035
1.212LysThr: 1.212 ± 0.04
1.968LysVal: 1.968 ± 0.058
0.182LysTrp: 0.182 ± 0.013
0.419LysTyr: 0.419 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.912LeuAla: 15.912 ± 0.159
0.546LeuCys: 0.546 ± 0.021
7.119LeuAsp: 7.119 ± 0.092
4.842LeuGlu: 4.842 ± 0.074
2.324LeuPhe: 2.324 ± 0.043
9.329LeuGly: 9.329 ± 0.117
1.88LeuHis: 1.88 ± 0.044
2.692LeuIle: 2.692 ± 0.052
1.498LeuLys: 1.498 ± 0.033
10.186LeuLeu: 10.186 ± 0.144
1.345LeuMet: 1.345 ± 0.036
1.414LeuAsn: 1.414 ± 0.036
5.76LeuPro: 5.76 ± 0.074
2.153LeuGln: 2.153 ± 0.048
7.47LeuArg: 7.47 ± 0.113
5.063LeuSer: 5.063 ± 0.076
6.737LeuThr: 6.737 ± 0.09
11.692LeuVal: 11.692 ± 0.128
1.273LeuTrp: 1.273 ± 0.035
1.472LeuTyr: 1.472 ± 0.037
0.0LeuXaa: 0.0 ± 0.0
Met
1.957MetAla: 1.957 ± 0.046
0.091MetCys: 0.091 ± 0.01
0.757MetAsp: 0.757 ± 0.025
0.502MetGlu: 0.502 ± 0.02
0.449MetPhe: 0.449 ± 0.023
1.16MetGly: 1.16 ± 0.03
0.319MetHis: 0.319 ± 0.016
0.618MetIle: 0.618 ± 0.023
0.298MetLys: 0.298 ± 0.018
1.613MetLeu: 1.613 ± 0.04
0.189MetMet: 0.189 ± 0.013
0.31MetAsn: 0.31 ± 0.017
0.953MetPro: 0.953 ± 0.03
0.401MetGln: 0.401 ± 0.019
1.191MetArg: 1.191 ± 0.032
1.363MetSer: 1.363 ± 0.03
1.607MetThr: 1.607 ± 0.038
1.246MetVal: 1.246 ± 0.032
0.196MetTrp: 0.196 ± 0.012
0.221MetTyr: 0.221 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.115AsnAla: 2.115 ± 0.049
0.108AsnCys: 0.108 ± 0.011
0.973AsnAsp: 0.973 ± 0.033
0.721AsnGlu: 0.721 ± 0.027
0.484AsnPhe: 0.484 ± 0.023
1.705AsnGly: 1.705 ± 0.058
0.329AsnHis: 0.329 ± 0.015
0.583AsnIle: 0.583 ± 0.026
0.347AsnLys: 0.347 ± 0.019
1.65AsnLeu: 1.65 ± 0.035
0.236AsnMet: 0.236 ± 0.015
0.385AsnAsn: 0.385 ± 0.021
1.314AsnPro: 1.314 ± 0.039
0.498AsnGln: 0.498 ± 0.022
1.022AsnArg: 1.022 ± 0.028
0.737AsnSer: 0.737 ± 0.029
0.942AsnThr: 0.942 ± 0.037
1.546AsnVal: 1.546 ± 0.038
0.234AsnTrp: 0.234 ± 0.017
0.352AsnTyr: 0.352 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
8.642ProAla: 8.642 ± 0.127
0.221ProCys: 0.221 ± 0.013
4.577ProAsp: 4.577 ± 0.06
3.252ProGlu: 3.252 ± 0.057
1.511ProPhe: 1.511 ± 0.043
5.705ProGly: 5.705 ± 0.07
1.06ProHis: 1.06 ± 0.029
1.558ProIle: 1.558 ± 0.036
0.931ProLys: 0.931 ± 0.035
4.745ProLeu: 4.745 ± 0.081
0.852ProMet: 0.852 ± 0.024
0.826ProAsn: 0.826 ± 0.031
2.821ProPro: 2.821 ± 0.064
1.475ProGln: 1.475 ± 0.035
3.543ProArg: 3.543 ± 0.057
3.487ProSer: 3.487 ± 0.063
4.452ProThr: 4.452 ± 0.086
5.724ProVal: 5.724 ± 0.074
0.892ProTrp: 0.892 ± 0.029
1.004ProTyr: 1.004 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.649GlnAla: 3.649 ± 0.056
0.118GlnCys: 0.118 ± 0.01
1.445GlnAsp: 1.445 ± 0.037
1.077GlnGlu: 1.077 ± 0.033
0.69GlnPhe: 0.69 ± 0.025
2.077GlnGly: 2.077 ± 0.046
0.641GlnHis: 0.641 ± 0.023
1.08GlnIle: 1.08 ± 0.031
0.477GlnLys: 0.477 ± 0.02
2.855GlnLeu: 2.855 ± 0.06
0.427GlnMet: 0.427 ± 0.02
0.474GlnAsn: 0.474 ± 0.019
1.588GlnPro: 1.588 ± 0.042
1.016GlnGln: 1.016 ± 0.035
2.21GlnArg: 2.21 ± 0.048
1.245GlnSer: 1.245 ± 0.033
1.448GlnThr: 1.448 ± 0.037
2.99GlnVal: 2.99 ± 0.049
0.537GlnTrp: 0.537 ± 0.023
0.577GlnTyr: 0.577 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
9.932ArgAla: 9.932 ± 0.129
0.352ArgCys: 0.352 ± 0.019
4.12ArgAsp: 4.12 ± 0.065
3.869ArgGlu: 3.869 ± 0.066
1.9ArgPhe: 1.9 ± 0.034
5.489ArgGly: 5.489 ± 0.075
1.534ArgHis: 1.534 ± 0.041
2.741ArgIle: 2.741 ± 0.053
1.091ArgLys: 1.091 ± 0.035
7.376ArgLeu: 7.376 ± 0.099
1.468ArgMet: 1.468 ± 0.033
1.073ArgAsn: 1.073 ± 0.033
3.946ArgPro: 3.946 ± 0.064
1.77ArgGln: 1.77 ± 0.038
6.815ArgArg: 6.815 ± 0.111
4.223ArgSer: 4.223 ± 0.064
5.011ArgThr: 5.011 ± 0.073
6.393ArgVal: 6.393 ± 0.091
1.408ArgTrp: 1.408 ± 0.039
1.528ArgTyr: 1.528 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
7.524SerAla: 7.524 ± 0.087
0.32SerCys: 0.32 ± 0.018
3.197SerAsp: 3.197 ± 0.055
2.219SerGlu: 2.219 ± 0.039
1.694SerPhe: 1.694 ± 0.036
6.003SerGly: 6.003 ± 0.097
0.954SerHis: 0.954 ± 0.029
1.95SerIle: 1.95 ± 0.041
1.005SerLys: 1.005 ± 0.032
4.906SerLeu: 4.906 ± 0.066
1.084SerMet: 1.084 ± 0.032
0.939SerAsn: 0.939 ± 0.027
3.065SerPro: 3.065 ± 0.051
1.373SerGln: 1.373 ± 0.037
3.518SerArg: 3.518 ± 0.055
4.024SerSer: 4.024 ± 0.096
4.325SerThr: 4.325 ± 0.092
5.053SerVal: 5.053 ± 0.077
0.953SerTrp: 0.953 ± 0.034
1.193SerTyr: 1.193 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
9.044ThrAla: 9.044 ± 0.136
0.366ThrCys: 0.366 ± 0.019
3.994ThrAsp: 3.994 ± 0.068
2.655ThrGlu: 2.655 ± 0.052
2.062ThrPhe: 2.062 ± 0.05
6.477ThrGly: 6.477 ± 0.096
1.171ThrHis: 1.171 ± 0.031
2.515ThrIle: 2.515 ± 0.052
1.338ThrLys: 1.338 ± 0.043
6.466ThrLeu: 6.466 ± 0.083
0.929ThrMet: 0.929 ± 0.031
1.264ThrAsn: 1.264 ± 0.041
4.523ThrPro: 4.523 ± 0.101
1.652ThrGln: 1.652 ± 0.038
3.939ThrArg: 3.939 ± 0.052
4.247ThrSer: 4.247 ± 0.078
5.299ThrThr: 5.299 ± 0.113
6.864ThrVal: 6.864 ± 0.124
1.236ThrTrp: 1.236 ± 0.039
1.552ThrTyr: 1.552 ± 0.04
0.0ThrXaa: 0.0 ± 0.0
Val
14.468ValAla: 14.468 ± 0.151
0.513ValCys: 0.513 ± 0.021
6.827ValAsp: 6.827 ± 0.086
5.278ValGlu: 5.278 ± 0.071
2.294ValPhe: 2.294 ± 0.052
8.654ValGly: 8.654 ± 0.099
1.892ValHis: 1.892 ± 0.055
3.171ValIle: 3.171 ± 0.061
1.668ValLys: 1.668 ± 0.059
11.116ValLeu: 11.116 ± 0.125
1.353ValMet: 1.353 ± 0.035
1.536ValAsn: 1.536 ± 0.038
5.784ValPro: 5.784 ± 0.076
2.383ValGln: 2.383 ± 0.045
7.287ValArg: 7.287 ± 0.094
4.97ValSer: 4.97 ± 0.074
7.059ValThr: 7.059 ± 0.12
12.346ValVal: 12.346 ± 0.137
1.203ValTrp: 1.203 ± 0.033
1.527ValTyr: 1.527 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.856TrpAla: 1.856 ± 0.044
0.124TrpCys: 0.124 ± 0.011
0.894TrpAsp: 0.894 ± 0.028
0.606TrpGlu: 0.606 ± 0.027
0.562TrpPhe: 0.562 ± 0.025
1.136TrpGly: 1.136 ± 0.032
0.355TrpHis: 0.355 ± 0.018
0.542TrpIle: 0.542 ± 0.024
0.297TrpLys: 0.297 ± 0.017
1.685TrpLeu: 1.685 ± 0.041
0.258TrpMet: 0.258 ± 0.015
0.404TrpAsn: 0.404 ± 0.02
0.804TrpPro: 0.804 ± 0.026
0.565TrpGln: 0.565 ± 0.021
1.272TrpArg: 1.272 ± 0.036
1.128TrpSer: 1.128 ± 0.037
1.169TrpThr: 1.169 ± 0.034
1.375TrpVal: 1.375 ± 0.036
0.441TrpTrp: 0.441 ± 0.02
0.324TrpTyr: 0.324 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.449TyrAla: 2.449 ± 0.042
0.108TyrCys: 0.108 ± 0.008
1.309TyrAsp: 1.309 ± 0.037
0.962TyrGlu: 0.962 ± 0.03
0.552TyrPhe: 0.552 ± 0.021
1.895TyrGly: 1.895 ± 0.054
0.265TyrHis: 0.265 ± 0.013
0.408TyrIle: 0.408 ± 0.02
0.34TyrLys: 0.34 ± 0.02
2.038TyrLeu: 2.038 ± 0.049
0.19TyrMet: 0.19 ± 0.014
0.365TyrAsn: 0.365 ± 0.017
0.954TyrPro: 0.954 ± 0.03
0.53TyrGln: 0.53 ± 0.022
1.568TyrArg: 1.568 ± 0.039
1.034TyrSer: 1.034 ± 0.034
1.17TyrThr: 1.17 ± 0.038
1.879TyrVal: 1.879 ± 0.041
0.318TyrTrp: 0.318 ± 0.017
0.419TyrTyr: 0.419 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3418 proteins (1165880 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski