Tessaracoccus antarcticus
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3179 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3M0GJ38|A0A3M0GJ38_9ACTN ABC transporter ATP-binding protein OS=Tessaracoccus antarcticus OX=2479848 GN=EAX62_03095 PE=3 SV=1
MM1 pKa = 7.28 AAVAGVLILLVGCTTPDD18 pKa = 3.36 PPAPSQEE25 pKa = 4.2 VTSPAITSTPASPPAGSATPSDD47 pKa = 4.25 VEE49 pKa = 4.12 EE50 pKa = 4.37 MDD52 pKa = 3.54 WGPFPPKK59 pKa = 10.67 NLDD62 pKa = 3.4 APKK65 pKa = 10.47 DD66 pKa = 3.6 LSDD69 pKa = 3.56 PQFPQTVLTFEE80 pKa = 5.14 LDD82 pKa = 3.78 SEE84 pKa = 4.65 SGDD87 pKa = 3.48 AASRR91 pKa = 11.84 DD92 pKa = 3.43 VFYY95 pKa = 11.11 ADD97 pKa = 3.62 RR98 pKa = 11.84 AAGVSMLASITLGRR112 pKa = 11.84 EE113 pKa = 4.05 SYY115 pKa = 10.39 RR116 pKa = 11.84 GAVEE120 pKa = 4.81 LLADD124 pKa = 4.21 PAYY127 pKa = 10.37 QGQAVCGHH135 pKa = 6.6 PTATPDD141 pKa = 3.48 QIEE144 pKa = 4.41 CVMVGAEE151 pKa = 4.11 HH152 pKa = 6.55 VLTVGTAAEE161 pKa = 4.43 SSLQDD166 pKa = 3.06 VAAFAEE172 pKa = 4.86 AIYY175 pKa = 10.7 AVLL178 pKa = 4.12
Molecular weight: 18.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.834
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.815
Protein with the highest isoelectric point:
>tr|A0A3M0GER3|A0A3M0GER3_9ACTN DUF4193 domain-containing protein OS=Tessaracoccus antarcticus OX=2479848 GN=EAX62_10275 PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.99 KK16 pKa = 9.77 HH17 pKa = 5.81 RR18 pKa = 11.84 KK19 pKa = 8.55 LLKK22 pKa = 8.44 KK23 pKa = 9.1 TRR25 pKa = 11.84 IQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 AGKK33 pKa = 9.79
Molecular weight: 4.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3179
0
3179
1084951
20
2223
341.3
36.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.05 ± 0.057
0.746 ± 0.012
6.136 ± 0.037
5.364 ± 0.04
3.125 ± 0.029
8.942 ± 0.034
2.29 ± 0.025
4.287 ± 0.03
2.253 ± 0.032
10.15 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.257 ± 0.018
2.282 ± 0.025
5.421 ± 0.031
3.067 ± 0.024
6.872 ± 0.042
5.889 ± 0.027
6.375 ± 0.036
8.937 ± 0.038
1.651 ± 0.022
1.908 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here