Hubei dimarhabdovirus virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMW1|A0A1L3KMW1_9VIRU Putative glycoprotein OS=Hubei dimarhabdovirus virus 2 OX=1922867 PE=4 SV=1
MM1 pKa = 7.41YY2 pKa = 10.33SLQGGLTDD10 pKa = 3.43VKK12 pKa = 10.72EE13 pKa = 4.37VLNSNPNYY21 pKa = 9.94LRR23 pKa = 11.84KK24 pKa = 9.67SYY26 pKa = 11.01QMSTDD31 pKa = 3.3EE32 pKa = 4.95EE33 pKa = 5.08KK34 pKa = 11.29YY35 pKa = 9.47STPPLSASLISEE47 pKa = 4.16DD48 pKa = 4.78CKK50 pKa = 11.37NLTDD54 pKa = 3.61QWSEE58 pKa = 3.88APIIPTDD65 pKa = 3.41GMVDD69 pKa = 3.04QGLILEE75 pKa = 4.33MEE77 pKa = 4.26IDD79 pKa = 3.72NEE81 pKa = 3.94KK82 pKa = 10.96DD83 pKa = 2.93HH84 pKa = 7.09ASRR87 pKa = 11.84IEE89 pKa = 3.86MSKK92 pKa = 10.97LKK94 pKa = 9.84TPPQDD99 pKa = 3.34DD100 pKa = 3.66QRR102 pKa = 11.84NEE104 pKa = 3.51TSKK107 pKa = 10.52RR108 pKa = 11.84SRR110 pKa = 11.84IITPEE115 pKa = 3.64EE116 pKa = 4.15DD117 pKa = 2.86KK118 pKa = 11.18WNIAIDD124 pKa = 3.76MGSLSLQDD132 pKa = 3.27KK133 pKa = 10.07MSFKK137 pKa = 10.69EE138 pKa = 3.97AFKK141 pKa = 10.65KK142 pKa = 10.3HH143 pKa = 4.2VDD145 pKa = 3.46AFVKK149 pKa = 10.55GCRR152 pKa = 11.84IPLNINYY159 pKa = 9.53DD160 pKa = 3.92IEE162 pKa = 4.78PMDD165 pKa = 3.52IQEE168 pKa = 4.15SKK170 pKa = 10.6IRR172 pKa = 11.84VIKK175 pKa = 9.86TSSPSEE181 pKa = 4.13GDD183 pKa = 3.61SMAMAQKK190 pKa = 10.63PPDD193 pKa = 4.01PEE195 pKa = 5.92LNDD198 pKa = 3.91TQKK201 pKa = 11.51KK202 pKa = 10.12LYY204 pKa = 10.51SLLLQGMEE212 pKa = 5.02LPLLSNPKK220 pKa = 9.87RR221 pKa = 11.84YY222 pKa = 8.8FVLKK226 pKa = 10.03IGKK229 pKa = 9.48DD230 pKa = 3.41EE231 pKa = 4.51FEE233 pKa = 4.68GNAVLDD239 pKa = 4.28VIIRR243 pKa = 11.84SPDD246 pKa = 3.14LDD248 pKa = 3.21IRR250 pKa = 11.84SLLRR254 pKa = 11.84RR255 pKa = 11.84VISISQQKK263 pKa = 10.61KK264 pKa = 9.57MILNLYY270 pKa = 9.22KK271 pKa = 9.82WDD273 pKa = 3.68AKK275 pKa = 10.55II276 pKa = 4.26

Molecular weight: 31.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMT3|A0A1L3KMT3_9VIRU Uncharacterized protein OS=Hubei dimarhabdovirus virus 2 OX=1922867 PE=4 SV=1
MM1 pKa = 7.2NRR3 pKa = 11.84LFKK6 pKa = 10.78SPRR9 pKa = 11.84KK10 pKa = 9.97RR11 pKa = 11.84KK12 pKa = 8.34DD13 pKa = 3.23TKK15 pKa = 10.8SSSSSDD21 pKa = 3.3SSSGSLSEE29 pKa = 4.92LPISVWIPPTSTGPVMEE46 pKa = 5.71DD47 pKa = 2.97YY48 pKa = 10.35WIRR51 pKa = 11.84PTSPPVSIVFDD62 pKa = 3.48SHH64 pKa = 5.49IVRR67 pKa = 11.84YY68 pKa = 10.01VKK70 pKa = 10.76LNLAITSNKK79 pKa = 8.89PIKK82 pKa = 10.63NHH84 pKa = 6.98DD85 pKa = 3.52EE86 pKa = 4.42MINMLSLIIDD96 pKa = 4.5HH97 pKa = 5.91YY98 pKa = 10.85QGPIVLKK105 pKa = 9.87PVILALYY112 pKa = 8.42GAAGMSLKK120 pKa = 10.13LDD122 pKa = 3.09RR123 pKa = 11.84GMRR126 pKa = 11.84SDD128 pKa = 3.64YY129 pKa = 10.93KK130 pKa = 10.76YY131 pKa = 10.99LSRR134 pKa = 11.84LDD136 pKa = 3.87QILMFHH142 pKa = 6.38TKK144 pKa = 9.95NRR146 pKa = 11.84DD147 pKa = 3.32YY148 pKa = 11.45SEE150 pKa = 4.99EE151 pKa = 3.73ICDD154 pKa = 3.73INWIKK159 pKa = 10.08TSSLGGNRR167 pKa = 11.84YY168 pKa = 9.89SIQYY172 pKa = 9.37SLTMEE177 pKa = 4.05KK178 pKa = 10.58SNRR181 pKa = 11.84KK182 pKa = 9.53GNDD185 pKa = 2.86FFSHH189 pKa = 4.49YY190 pKa = 9.84RR191 pKa = 11.84KK192 pKa = 10.09YY193 pKa = 11.11SRR195 pKa = 11.84TEE197 pKa = 3.2KK198 pKa = 10.5SMSMEE203 pKa = 4.13VIKK206 pKa = 10.66EE207 pKa = 3.65ILNIDD212 pKa = 3.64RR213 pKa = 11.84MEE215 pKa = 4.65LEE217 pKa = 3.89SDD219 pKa = 3.46TAVFLL224 pKa = 4.51

Molecular weight: 25.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3574

224

2119

714.8

81.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.302 ± 0.406

1.595 ± 0.376

6.072 ± 0.438

5.624 ± 0.326

4.001 ± 0.443

5.092 ± 0.28

2.434 ± 0.368

8.73 ± 0.322

7.387 ± 0.318

9.625 ± 0.52

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.882 ± 0.393

5.988 ± 0.31

4.477 ± 0.399

2.462 ± 0.377

4.393 ± 0.733

9.569 ± 0.645

5.036 ± 0.467

5.232 ± 0.379

1.623 ± 0.228

4.477 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski