Herbihabitans rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Herbihabitans

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6595 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7KII4|A0A4Q7KII4_9PSEU Methyltransferase family protein OS=Herbihabitans rhizosphaerae OX=1872711 GN=EV193_10886 PE=4 SV=1
MM1 pKa = 7.37SVTPATEE8 pKa = 4.15PAPQGTPCWLEE19 pKa = 3.93LGTTNLPACTDD30 pKa = 4.3FYY32 pKa = 11.61AGLLGWEE39 pKa = 4.18YY40 pKa = 11.16QPDD43 pKa = 3.55DD44 pKa = 3.8SGYY47 pKa = 8.85LTAHH51 pKa = 6.7TDD53 pKa = 3.69GVPAAGLYY61 pKa = 9.69AFPDD65 pKa = 4.65GPPLPTTWTLYY76 pKa = 10.6LSVNNVANTAQRR88 pKa = 11.84VTMLDD93 pKa = 3.17GQVAVEE99 pKa = 3.92PRR101 pKa = 11.84EE102 pKa = 3.87IPGQGRR108 pKa = 11.84LMIAADD114 pKa = 3.64PTGALIGFWEE124 pKa = 4.94PATMWPFAQGGAGAFAWADD143 pKa = 3.36LHH145 pKa = 5.55TTDD148 pKa = 5.58GKK150 pKa = 10.98LADD153 pKa = 4.12EE154 pKa = 4.88FFVSLFSFDD163 pKa = 3.66TEE165 pKa = 4.38QVGDD169 pKa = 4.53GIDD172 pKa = 3.4YY173 pKa = 10.33DD174 pKa = 4.07YY175 pKa = 11.15TIWTLDD181 pKa = 3.45GPSLLGRR188 pKa = 11.84LRR190 pKa = 11.84MGAEE194 pKa = 4.57FEE196 pKa = 4.88PGTPPHH202 pKa = 6.07WMNYY206 pKa = 8.23VGVSAALGVDD216 pKa = 4.23LAATRR221 pKa = 11.84AIQLGGRR228 pKa = 11.84LHH230 pKa = 7.2AEE232 pKa = 4.43PFDD235 pKa = 3.84SPYY238 pKa = 11.1GRR240 pKa = 11.84IAVIEE245 pKa = 4.15DD246 pKa = 3.25VAGGVLSLVDD256 pKa = 3.33TSRR259 pKa = 11.84RR260 pKa = 11.84TPIAEE265 pKa = 3.99EE266 pKa = 4.91DD267 pKa = 3.3IGTAAVDD274 pKa = 4.64DD275 pKa = 5.3PYY277 pKa = 11.99DD278 pKa = 3.77DD279 pKa = 3.85

Molecular weight: 29.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7L7P8|A0A4Q7L7P8_9PSEU 4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Herbihabitans rhizosphaerae OX=1872711 GN=ispH PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.46LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.07LGKK33 pKa = 9.87

Molecular weight: 4.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6595

0

6595

2000804

29

3672

303.4

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.841 ± 0.041

0.797 ± 0.009

6.29 ± 0.024

5.592 ± 0.03

2.808 ± 0.018

9.131 ± 0.031

2.219 ± 0.014

3.726 ± 0.02

2.347 ± 0.027

9.999 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.853 ± 0.011

2.049 ± 0.019

5.893 ± 0.027

2.7 ± 0.019

7.872 ± 0.035

5.202 ± 0.023

6.19 ± 0.023

8.959 ± 0.03

1.537 ± 0.014

1.995 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski