Phialemoniopsis curvata
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12104 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A507B8F9|A0A507B8F9_9PEZI Uncharacterized protein OS=Phialemoniopsis curvata OX=1093900 GN=E0L32_004112 PE=3 SV=1
MM1 pKa = 7.72 LWMHH5 pKa = 6.33 WMLWMHH11 pKa = 6.27 WMHH14 pKa = 6.03 WMLWILWMLWMLWMLWMLWMLWMHH38 pKa = 6.25 WMLWMHH44 pKa = 6.94 WLDD47 pKa = 4.11 ALSALDD53 pKa = 4.06 TLDD56 pKa = 5.24 ALDD59 pKa = 5.44 ALDD62 pKa = 5.79 ALDD65 pKa = 5.79 ALDD68 pKa = 5.79 ALDD71 pKa = 5.79 ALDD74 pKa = 5.79 ALDD77 pKa = 5.79 ALDD80 pKa = 5.38 ALDD83 pKa = 4.65 ALGSTGEE90 pKa = 4.06 YY91 pKa = 10.88 NMYY94 pKa = 10.91
Molecular weight: 11.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.478
ProMoST 3.935
Dawson 3.745
Bjellqvist 3.91
Wikipedia 3.77
Rodwell 3.541
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.884
DTASelect 4.202
Thurlkill 3.579
EMBOSS 3.77
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.764
Protein with the highest isoelectric point:
>tr|A0A507AZM2|A0A507AZM2_9PEZI Uncharacterized protein OS=Phialemoniopsis curvata OX=1093900 GN=E0L32_008706 PE=4 SV=1
MM1 pKa = 7.41 AQPASTPAPQHH12 pKa = 5.1 QRR14 pKa = 11.84 ANSTNPRR21 pKa = 11.84 PGTSAFPRR29 pKa = 11.84 ANSQSQTRR37 pKa = 11.84 PQQQQQQQQQQQQQQQQQQNRR58 pKa = 11.84 TRR60 pKa = 11.84 SKK62 pKa = 8.06 RR63 pKa = 11.84 TTGARR68 pKa = 11.84 SSTGSGTSATAAPARR83 pKa = 11.84 PPAPSTKK90 pKa = 9.9 QVNQTFSITMMSSPVNPVPFPLPIPLGISMLVMPTGGKK128 pKa = 9.56 SGSQSGRR135 pKa = 11.84 GG136 pKa = 3.36
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.574
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.31
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.172
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12104
0
12104
6446461
34
5835
532.6
58.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.615 ± 0.024
1.284 ± 0.008
5.813 ± 0.016
6.004 ± 0.021
3.619 ± 0.011
7.417 ± 0.021
2.4 ± 0.009
4.476 ± 0.014
4.476 ± 0.019
8.823 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.194 ± 0.008
3.292 ± 0.01
6.234 ± 0.025
4.014 ± 0.015
6.317 ± 0.019
7.877 ± 0.025
5.717 ± 0.018
6.279 ± 0.015
1.487 ± 0.008
2.662 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here