Sphingobacterium spiritivorum ATCC 33861

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; Sphingobacterium spiritivorum

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7VS14|D7VS14_9SPHI Hydrolase NUDIX family OS=Sphingobacterium spiritivorum ATCC 33861 OX=525373 GN=HMPREF0766_13768 PE=3 SV=1
MM1 pKa = 7.58EE2 pKa = 4.66WNKK5 pKa = 10.32IKK7 pKa = 10.88ADD9 pKa = 3.66TQNIYY14 pKa = 10.29PEE16 pKa = 3.89NSITLFLMDD25 pKa = 4.4TDD27 pKa = 3.71SGKK30 pKa = 9.53PATCWVDD37 pKa = 3.15KK38 pKa = 10.97AYY40 pKa = 10.65ADD42 pKa = 3.59YY43 pKa = 9.27PYY45 pKa = 10.73KK46 pKa = 9.95QEE48 pKa = 4.77CMFNCFISVDD58 pKa = 3.92LLNDD62 pKa = 3.38EE63 pKa = 4.64FNLRR67 pKa = 11.84NEE69 pKa = 4.27DD70 pKa = 3.54LDD72 pKa = 4.12YY73 pKa = 11.82GDD75 pKa = 4.15IEE77 pKa = 6.18DD78 pKa = 5.34YY79 pKa = 8.99FTQQLRR85 pKa = 11.84EE86 pKa = 3.97VCVCHH91 pKa = 5.87IVARR95 pKa = 11.84ITTDD99 pKa = 2.82SGISIEE105 pKa = 4.32MYY107 pKa = 10.62VDD109 pKa = 3.72DD110 pKa = 4.83VEE112 pKa = 4.67SAIGKK117 pKa = 9.4LNEE120 pKa = 4.85LEE122 pKa = 4.54ADD124 pKa = 3.77PNRR127 pKa = 11.84LVNFDD132 pKa = 4.73CEE134 pKa = 4.46ISDD137 pKa = 4.81DD138 pKa = 4.21EE139 pKa = 4.55NWDD142 pKa = 3.75NVDD145 pKa = 3.35NLLNDD150 pKa = 3.61VEE152 pKa = 4.36

Molecular weight: 17.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7VP22|D7VP22_9SPHI Peptidase_M14 domain-containing protein OS=Sphingobacterium spiritivorum ATCC 33861 OX=525373 GN=HMPREF0766_12742 PE=4 SV=1
MM1 pKa = 7.85PVPKK5 pKa = 9.98IQQRR9 pKa = 11.84PSSLPAQRR17 pKa = 11.84RR18 pKa = 11.84NPKK21 pKa = 8.82NQNIQKK27 pKa = 9.52VARR30 pKa = 11.84ANPVKK35 pKa = 10.38NAKK38 pKa = 7.97VTNAGAVVPTALAGAVLQPLPP59 pKa = 4.09

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4471

0

4471

1536412

39

3807

343.6

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.005 ± 0.041

0.706 ± 0.01

5.478 ± 0.024

5.959 ± 0.042

4.845 ± 0.029

6.66 ± 0.034

1.813 ± 0.017

7.448 ± 0.028

7.003 ± 0.036

9.354 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.291 ± 0.018

5.712 ± 0.041

3.595 ± 0.022

3.929 ± 0.025

4.065 ± 0.022

6.606 ± 0.032

5.735 ± 0.038

6.239 ± 0.027

1.16 ± 0.014

4.396 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski