Leishmania braziliensis
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8084 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4HBL5|A4HBL5_LEIBR Uncharacterized protein OS=Leishmania braziliensis OX=5660 GN=LBRM_21_0220 PE=4 SV=1
MM1 pKa = 8.05 ADD3 pKa = 3.62 PVLQLLEE10 pKa = 4.56 IEE12 pKa = 4.75 LLGEE16 pKa = 4.26 NPDD19 pKa = 3.79 AYY21 pKa = 9.67 TSQFQWRR28 pKa = 11.84 MRR30 pKa = 11.84 MEE32 pKa = 4.5 AAASLPDD39 pKa = 3.58 AVSVSFVWVGSASSSQYY56 pKa = 11.27 DD57 pKa = 3.38 QVLDD61 pKa = 3.95 DD62 pKa = 5.15 FEE64 pKa = 4.87 VGPLEE69 pKa = 4.07 QGVTEE74 pKa = 4.69 FTLEE78 pKa = 3.72 CDD80 pKa = 3.68 APQMEE85 pKa = 5.13 LVPTEE90 pKa = 4.26 DD91 pKa = 3.46 VLGVTILLISFQYY104 pKa = 10.06 RR105 pKa = 11.84 RR106 pKa = 11.84 QEE108 pKa = 3.93 FLRR111 pKa = 11.84 VGYY114 pKa = 7.42 YY115 pKa = 8.64 TQVAYY120 pKa = 10.48 FDD122 pKa = 4.06 AQLNQNPPPLPQRR135 pKa = 11.84 EE136 pKa = 4.09 LLGRR140 pKa = 11.84 FVAMPQPAVTVTPIEE155 pKa = 4.05 WGSYY159 pKa = 8.93 GPQPATT165 pKa = 3.52
Molecular weight: 18.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.656
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.681
Grimsley 3.567
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.075
Thurlkill 3.694
EMBOSS 3.719
Sillero 3.961
Patrickios 1.863
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.862
Protein with the highest isoelectric point:
>tr|A4H855|A4H855_LEIBR Uncharacterized protein OS=Leishmania braziliensis OX=5660 GN=LBRM_15_0860 PE=4 SV=1
MM1 pKa = 7.6 PSWTRR6 pKa = 11.84 RR7 pKa = 11.84 TRR9 pKa = 11.84 RR10 pKa = 11.84 WSSSWRR16 pKa = 11.84 SGRR19 pKa = 11.84 PAPPAWMPSAATCLSALHH37 pKa = 6.46 GWRR40 pKa = 11.84 VSTRR44 pKa = 11.84 SWARR48 pKa = 11.84 RR49 pKa = 11.84 TRR51 pKa = 11.84 RR52 pKa = 11.84 WSSSWRR58 pKa = 11.84 SGRR61 pKa = 11.84 PAPPAWMPSAATCLSALRR79 pKa = 11.84 GWRR82 pKa = 11.84 VSTRR86 pKa = 11.84 SWARR90 pKa = 11.84 HH91 pKa = 4.03 MPSWTRR97 pKa = 11.84 RR98 pKa = 11.84 TRR100 pKa = 11.84 RR101 pKa = 11.84 WSSSWRR107 pKa = 11.84 SGRR110 pKa = 11.84 PAPPAWTPSAATCLSALHH128 pKa = 6.46 GWRR131 pKa = 11.84 VSTRR135 pKa = 11.84 SWARR139 pKa = 11.84 HH140 pKa = 4.03 MPSWTRR146 pKa = 11.84 RR147 pKa = 11.84 TRR149 pKa = 11.84 RR150 pKa = 11.84 WSSSWRR156 pKa = 11.84 SGRR159 pKa = 11.84 PAPPAWMPSAATCLSALSGWRR180 pKa = 11.84 VSTRR184 pKa = 11.84 SWARR188 pKa = 11.84 HH189 pKa = 4.03 MPSWTRR195 pKa = 11.84 RR196 pKa = 11.84 TRR198 pKa = 11.84 RR199 pKa = 11.84 WSSSWRR205 pKa = 11.84 SGRR208 pKa = 11.84 PAPPAWTPSAATCLSALRR226 pKa = 11.84 GWRR229 pKa = 11.84 VSTRR233 pKa = 11.84 SWARR237 pKa = 11.84 HH238 pKa = 4.03 MPSWTRR244 pKa = 11.84 RR245 pKa = 11.84 TRR247 pKa = 11.84 RR248 pKa = 11.84 WSSSWRR254 pKa = 11.84 SGRR257 pKa = 11.84 PAPPAWMPSAATCLSALHH275 pKa = 6.46 GWRR278 pKa = 11.84 VSTRR282 pKa = 11.84 SWARR286 pKa = 11.84 HH287 pKa = 4.03 MPSWTRR293 pKa = 11.84 RR294 pKa = 11.84 TRR296 pKa = 11.84 RR297 pKa = 11.84 WSSSWRR303 pKa = 11.84 SGRR306 pKa = 11.84 PAPPAWMPSAATCLSALHH324 pKa = 6.46 GWRR327 pKa = 11.84 VSTRR331 pKa = 11.84 SWARR335 pKa = 11.84 RR336 pKa = 11.84 TRR338 pKa = 11.84 RR339 pKa = 11.84 WSSSWRR345 pKa = 11.84 SGRR348 pKa = 11.84 PAPPTWMPSAATCLSALHH366 pKa = 6.46 GWRR369 pKa = 11.84 VSTRR373 pKa = 11.84 SWARR377 pKa = 11.84 HH378 pKa = 4.03 MPSWTRR384 pKa = 11.84 RR385 pKa = 11.84 TRR387 pKa = 11.84 RR388 pKa = 11.84 WSSSWRR394 pKa = 11.84 SGRR397 pKa = 11.84 PAPPAWMPSAATCLSALHH415 pKa = 6.46 GWRR418 pKa = 11.84 VSTRR422 pKa = 11.84 SWARR426 pKa = 11.84 RR427 pKa = 11.84 TRR429 pKa = 11.84 RR430 pKa = 11.84 WSSSWRR436 pKa = 11.84 SGRR439 pKa = 11.84 PAPPAWMPSAATCMSACAWRR459 pKa = 11.84 VSTRR463 pKa = 11.84 SWARR467 pKa = 11.84 RR468 pKa = 11.84 TRR470 pKa = 11.84 RR471 pKa = 11.84 WSSSWRR477 pKa = 11.84 SGRR480 pKa = 11.84 PAPPAWMPSAATCLSALRR498 pKa = 11.84 GWRR501 pKa = 11.84 VSTRR505 pKa = 11.84 SWARR509 pKa = 11.84 HH510 pKa = 4.03 MPSWTRR516 pKa = 11.84 RR517 pKa = 11.84 TRR519 pKa = 11.84 RR520 pKa = 11.84 WSSSWRR526 pKa = 11.84 SGRR529 pKa = 11.84 PAPPAWMPSAATCLSALRR547 pKa = 11.84 GWRR550 pKa = 11.84 VSTRR554 pKa = 11.84 SWARR558 pKa = 11.84 HH559 pKa = 4.03 MPSWTRR565 pKa = 11.84 RR566 pKa = 11.84 TRR568 pKa = 11.84 RR569 pKa = 11.84 WSSSWRR575 pKa = 11.84 SGRR578 pKa = 11.84 PAPPAWMPSAATCLSALHH596 pKa = 6.46 GWRR599 pKa = 11.84 VSTRR603 pKa = 11.84 SWARR607 pKa = 11.84 RR608 pKa = 11.84 TRR610 pKa = 11.84 RR611 pKa = 11.84 WSSSWRR617 pKa = 11.84 SGRR620 pKa = 11.84 PAPPAWMPSAATCLSALHH638 pKa = 6.46 GWRR641 pKa = 11.84 VSTRR645 pKa = 11.84 SWARR649 pKa = 11.84 HH650 pKa = 4.03 MPSWTRR656 pKa = 11.84 RR657 pKa = 11.84 TRR659 pKa = 11.84 RR660 pKa = 11.84 WSSSWRR666 pKa = 11.84 SGRR669 pKa = 11.84 PAPPAWTPSAATCLSALHH687 pKa = 6.46 GWRR690 pKa = 11.84 VSTRR694 pKa = 11.84 SWARR698 pKa = 11.84 HH699 pKa = 4.03 MPSWTRR705 pKa = 11.84 RR706 pKa = 11.84 TRR708 pKa = 11.84 RR709 pKa = 11.84 WSSSWRR715 pKa = 11.84 SGRR718 pKa = 11.84 PAPPAWMPSAATCLSALSGWRR739 pKa = 11.84 VSTRR743 pKa = 11.84 SWARR747 pKa = 11.84 HH748 pKa = 4.03 MPSWTRR754 pKa = 11.84 RR755 pKa = 11.84 TRR757 pKa = 11.84 RR758 pKa = 11.84 WSSSWRR764 pKa = 11.84 SGRR767 pKa = 11.84 PAPPAWMPSAATCLSALRR785 pKa = 11.84 GWRR788 pKa = 11.84 VSTRR792 pKa = 11.84 SWARR796 pKa = 11.84 HH797 pKa = 4.03 MPSWTRR803 pKa = 11.84 RR804 pKa = 11.84 TRR806 pKa = 11.84 RR807 pKa = 11.84 WSSSWRR813 pKa = 11.84 SGRR816 pKa = 11.84 PAPPAWMPSAATCLSALHH834 pKa = 6.46 GWRR837 pKa = 11.84 VSTRR841 pKa = 11.84 SWARR845 pKa = 11.84 HH846 pKa = 4.03 MPSWTRR852 pKa = 11.84 RR853 pKa = 11.84 TRR855 pKa = 11.84 RR856 pKa = 11.84 WSSSWRR862 pKa = 11.84 SGRR865 pKa = 11.84 PAPPAWTPSAATCLSALRR883 pKa = 11.84 GWRR886 pKa = 11.84 VSTRR890 pKa = 11.84 SWARR894 pKa = 11.84 HH895 pKa = 4.03 MPSWTRR901 pKa = 11.84 RR902 pKa = 11.84 TRR904 pKa = 11.84 RR905 pKa = 11.84 WSSSWRR911 pKa = 11.84 SGRR914 pKa = 11.84 PAPPAWMPSAATCLSALHH932 pKa = 6.46 GWRR935 pKa = 11.84 VSTRR939 pKa = 11.84 SWTRR943 pKa = 11.84 RR944 pKa = 11.84 TRR946 pKa = 11.84 RR947 pKa = 11.84 WSSSWRR953 pKa = 11.84 SGRR956 pKa = 11.84 PAPPAWMPSAATCLSALHH974 pKa = 6.46 GWRR977 pKa = 11.84 VSTRR981 pKa = 11.84 SWARR985 pKa = 11.84 HH986 pKa = 4.03 MPSWTRR992 pKa = 11.84 RR993 pKa = 11.84 TRR995 pKa = 11.84 RR996 pKa = 11.84 WSSSWRR1002 pKa = 11.84 SGRR1005 pKa = 11.84 PAPPAWMPSAATCLSALHH1023 pKa = 6.46 GWRR1026 pKa = 11.84 VSTRR1030 pKa = 11.84 SWARR1034 pKa = 11.84 HH1035 pKa = 4.03 MPSWTRR1041 pKa = 11.84 RR1042 pKa = 11.84 TRR1044 pKa = 11.84 RR1045 pKa = 11.84 WSSSWRR1051 pKa = 11.84 SGRR1054 pKa = 11.84 PAPPAWMPSAATCLSALRR1072 pKa = 11.84 GWRR1075 pKa = 11.84 VSTRR1079 pKa = 11.84 SWARR1083 pKa = 11.84 HH1084 pKa = 4.03 MPSWTRR1090 pKa = 11.84 RR1091 pKa = 11.84 TRR1093 pKa = 11.84 RR1094 pKa = 11.84 WSSSWRR1100 pKa = 11.84 SGRR1103 pKa = 11.84 PAPPAWMPSAATCLSALHH1121 pKa = 6.46 GWRR1124 pKa = 11.84 VSTRR1128 pKa = 11.84 SWARR1132 pKa = 11.84 HH1133 pKa = 4.03 MPSWTRR1139 pKa = 11.84 RR1140 pKa = 11.84 TRR1142 pKa = 11.84 RR1143 pKa = 11.84 WSSSWRR1149 pKa = 11.84 SGRR1152 pKa = 11.84 PAPPAWMPSAATCLSALRR1170 pKa = 11.84 GWRR1173 pKa = 11.84 VSTRR1177 pKa = 11.84 SWARR1181 pKa = 11.84 HH1182 pKa = 4.03 MPSWTRR1188 pKa = 11.84 RR1189 pKa = 11.84 TRR1191 pKa = 11.84 RR1192 pKa = 11.84 WSSSWRR1198 pKa = 11.84 SGRR1201 pKa = 11.84 PAPPAWMPSAATCLSALRR1219 pKa = 11.84 GWRR1222 pKa = 11.84 VSTRR1226 pKa = 11.84 SWTRR1230 pKa = 11.84 RR1231 pKa = 11.84 TRR1233 pKa = 11.84 RR1234 pKa = 11.84 WSSSWRR1240 pKa = 11.84 SGRR1243 pKa = 11.84 PAPPAWMPSAATCLSALSGWRR1264 pKa = 11.84 VSTRR1268 pKa = 11.84 SWARR1272 pKa = 11.84 HH1273 pKa = 4.03 MPSWTRR1279 pKa = 11.84 RR1280 pKa = 11.84 TRR1282 pKa = 11.84 RR1283 pKa = 11.84 WSSSWRR1289 pKa = 11.84 SGRR1292 pKa = 11.84 PAPPAWMPSAATCLSALRR1310 pKa = 11.84 GWRR1313 pKa = 11.84 VSTRR1317 pKa = 11.84 SWARR1321 pKa = 11.84 HH1322 pKa = 4.03 MPSWTRR1328 pKa = 11.84 RR1329 pKa = 11.84 TRR1331 pKa = 11.84 RR1332 pKa = 11.84 WSSSWRR1338 pKa = 11.84 SGRR1341 pKa = 11.84 PAPPAWTPSAATCLSALHH1359 pKa = 6.46 GWRR1362 pKa = 11.84 VSTRR1366 pKa = 11.84 SWARR1370 pKa = 11.84 HH1371 pKa = 4.03 MPSWTRR1377 pKa = 11.84 RR1378 pKa = 11.84 TRR1380 pKa = 11.84 RR1381 pKa = 11.84 WSSSWRR1387 pKa = 11.84 SGRR1390 pKa = 11.84 PAPPAWMPSAATCLSALRR1408 pKa = 11.84 GWRR1411 pKa = 11.84 VSTRR1415 pKa = 11.84 SWASLMQGG1423 pKa = 3.17
Molecular weight: 164.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.384
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.076
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.18
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8084
0
8084
5077572
14
6733
628.1
68.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.166 ± 0.042
1.903 ± 0.012
4.811 ± 0.017
6.03 ± 0.033
3.007 ± 0.021
6.303 ± 0.022
2.764 ± 0.013
3.152 ± 0.019
3.388 ± 0.023
9.42 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.011
2.768 ± 0.015
5.717 ± 0.031
4.154 ± 0.018
7.114 ± 0.024
8.91 ± 0.054
6.289 ± 0.024
7.25 ± 0.021
1.1 ± 0.009
2.464 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here