Leishmania braziliensis

Taxonomy: cellular organisms; Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Leishmaniinae; Leishmania; Viannia; Leishmania braziliensis species complex

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8084 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A4HBL5|A4HBL5_LEIBR Uncharacterized protein OS=Leishmania braziliensis OX=5660 GN=LBRM_21_0220 PE=4 SV=1
MM1 pKa = 8.05ADD3 pKa = 3.62PVLQLLEE10 pKa = 4.56IEE12 pKa = 4.75LLGEE16 pKa = 4.26NPDD19 pKa = 3.79AYY21 pKa = 9.67TSQFQWRR28 pKa = 11.84MRR30 pKa = 11.84MEE32 pKa = 4.5AAASLPDD39 pKa = 3.58AVSVSFVWVGSASSSQYY56 pKa = 11.27DD57 pKa = 3.38QVLDD61 pKa = 3.95DD62 pKa = 5.15FEE64 pKa = 4.87VGPLEE69 pKa = 4.07QGVTEE74 pKa = 4.69FTLEE78 pKa = 3.72CDD80 pKa = 3.68APQMEE85 pKa = 5.13LVPTEE90 pKa = 4.26DD91 pKa = 3.46VLGVTILLISFQYY104 pKa = 10.06RR105 pKa = 11.84RR106 pKa = 11.84QEE108 pKa = 3.93FLRR111 pKa = 11.84VGYY114 pKa = 7.42YY115 pKa = 8.64TQVAYY120 pKa = 10.48FDD122 pKa = 4.06AQLNQNPPPLPQRR135 pKa = 11.84EE136 pKa = 4.09LLGRR140 pKa = 11.84FVAMPQPAVTVTPIEE155 pKa = 4.05WGSYY159 pKa = 8.93GPQPATT165 pKa = 3.52

Molecular weight:
18.48 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A4H855|A4H855_LEIBR Uncharacterized protein OS=Leishmania braziliensis OX=5660 GN=LBRM_15_0860 PE=4 SV=1
MM1 pKa = 7.6PSWTRR6 pKa = 11.84RR7 pKa = 11.84TRR9 pKa = 11.84RR10 pKa = 11.84WSSSWRR16 pKa = 11.84SGRR19 pKa = 11.84PAPPAWMPSAATCLSALHH37 pKa = 6.46GWRR40 pKa = 11.84VSTRR44 pKa = 11.84SWARR48 pKa = 11.84RR49 pKa = 11.84TRR51 pKa = 11.84RR52 pKa = 11.84WSSSWRR58 pKa = 11.84SGRR61 pKa = 11.84PAPPAWMPSAATCLSALRR79 pKa = 11.84GWRR82 pKa = 11.84VSTRR86 pKa = 11.84SWARR90 pKa = 11.84HH91 pKa = 4.03MPSWTRR97 pKa = 11.84RR98 pKa = 11.84TRR100 pKa = 11.84RR101 pKa = 11.84WSSSWRR107 pKa = 11.84SGRR110 pKa = 11.84PAPPAWTPSAATCLSALHH128 pKa = 6.46GWRR131 pKa = 11.84VSTRR135 pKa = 11.84SWARR139 pKa = 11.84HH140 pKa = 4.03MPSWTRR146 pKa = 11.84RR147 pKa = 11.84TRR149 pKa = 11.84RR150 pKa = 11.84WSSSWRR156 pKa = 11.84SGRR159 pKa = 11.84PAPPAWMPSAATCLSALSGWRR180 pKa = 11.84VSTRR184 pKa = 11.84SWARR188 pKa = 11.84HH189 pKa = 4.03MPSWTRR195 pKa = 11.84RR196 pKa = 11.84TRR198 pKa = 11.84RR199 pKa = 11.84WSSSWRR205 pKa = 11.84SGRR208 pKa = 11.84PAPPAWTPSAATCLSALRR226 pKa = 11.84GWRR229 pKa = 11.84VSTRR233 pKa = 11.84SWARR237 pKa = 11.84HH238 pKa = 4.03MPSWTRR244 pKa = 11.84RR245 pKa = 11.84TRR247 pKa = 11.84RR248 pKa = 11.84WSSSWRR254 pKa = 11.84SGRR257 pKa = 11.84PAPPAWMPSAATCLSALHH275 pKa = 6.46GWRR278 pKa = 11.84VSTRR282 pKa = 11.84SWARR286 pKa = 11.84HH287 pKa = 4.03MPSWTRR293 pKa = 11.84RR294 pKa = 11.84TRR296 pKa = 11.84RR297 pKa = 11.84WSSSWRR303 pKa = 11.84SGRR306 pKa = 11.84PAPPAWMPSAATCLSALHH324 pKa = 6.46GWRR327 pKa = 11.84VSTRR331 pKa = 11.84SWARR335 pKa = 11.84RR336 pKa = 11.84TRR338 pKa = 11.84RR339 pKa = 11.84WSSSWRR345 pKa = 11.84SGRR348 pKa = 11.84PAPPTWMPSAATCLSALHH366 pKa = 6.46GWRR369 pKa = 11.84VSTRR373 pKa = 11.84SWARR377 pKa = 11.84HH378 pKa = 4.03MPSWTRR384 pKa = 11.84RR385 pKa = 11.84TRR387 pKa = 11.84RR388 pKa = 11.84WSSSWRR394 pKa = 11.84SGRR397 pKa = 11.84PAPPAWMPSAATCLSALHH415 pKa = 6.46GWRR418 pKa = 11.84VSTRR422 pKa = 11.84SWARR426 pKa = 11.84RR427 pKa = 11.84TRR429 pKa = 11.84RR430 pKa = 11.84WSSSWRR436 pKa = 11.84SGRR439 pKa = 11.84PAPPAWMPSAATCMSACAWRR459 pKa = 11.84VSTRR463 pKa = 11.84SWARR467 pKa = 11.84RR468 pKa = 11.84TRR470 pKa = 11.84RR471 pKa = 11.84WSSSWRR477 pKa = 11.84SGRR480 pKa = 11.84PAPPAWMPSAATCLSALRR498 pKa = 11.84GWRR501 pKa = 11.84VSTRR505 pKa = 11.84SWARR509 pKa = 11.84HH510 pKa = 4.03MPSWTRR516 pKa = 11.84RR517 pKa = 11.84TRR519 pKa = 11.84RR520 pKa = 11.84WSSSWRR526 pKa = 11.84SGRR529 pKa = 11.84PAPPAWMPSAATCLSALRR547 pKa = 11.84GWRR550 pKa = 11.84VSTRR554 pKa = 11.84SWARR558 pKa = 11.84HH559 pKa = 4.03MPSWTRR565 pKa = 11.84RR566 pKa = 11.84TRR568 pKa = 11.84RR569 pKa = 11.84WSSSWRR575 pKa = 11.84SGRR578 pKa = 11.84PAPPAWMPSAATCLSALHH596 pKa = 6.46GWRR599 pKa = 11.84VSTRR603 pKa = 11.84SWARR607 pKa = 11.84RR608 pKa = 11.84TRR610 pKa = 11.84RR611 pKa = 11.84WSSSWRR617 pKa = 11.84SGRR620 pKa = 11.84PAPPAWMPSAATCLSALHH638 pKa = 6.46GWRR641 pKa = 11.84VSTRR645 pKa = 11.84SWARR649 pKa = 11.84HH650 pKa = 4.03MPSWTRR656 pKa = 11.84RR657 pKa = 11.84TRR659 pKa = 11.84RR660 pKa = 11.84WSSSWRR666 pKa = 11.84SGRR669 pKa = 11.84PAPPAWTPSAATCLSALHH687 pKa = 6.46GWRR690 pKa = 11.84VSTRR694 pKa = 11.84SWARR698 pKa = 11.84HH699 pKa = 4.03MPSWTRR705 pKa = 11.84RR706 pKa = 11.84TRR708 pKa = 11.84RR709 pKa = 11.84WSSSWRR715 pKa = 11.84SGRR718 pKa = 11.84PAPPAWMPSAATCLSALSGWRR739 pKa = 11.84VSTRR743 pKa = 11.84SWARR747 pKa = 11.84HH748 pKa = 4.03MPSWTRR754 pKa = 11.84RR755 pKa = 11.84TRR757 pKa = 11.84RR758 pKa = 11.84WSSSWRR764 pKa = 11.84SGRR767 pKa = 11.84PAPPAWMPSAATCLSALRR785 pKa = 11.84GWRR788 pKa = 11.84VSTRR792 pKa = 11.84SWARR796 pKa = 11.84HH797 pKa = 4.03MPSWTRR803 pKa = 11.84RR804 pKa = 11.84TRR806 pKa = 11.84RR807 pKa = 11.84WSSSWRR813 pKa = 11.84SGRR816 pKa = 11.84PAPPAWMPSAATCLSALHH834 pKa = 6.46GWRR837 pKa = 11.84VSTRR841 pKa = 11.84SWARR845 pKa = 11.84HH846 pKa = 4.03MPSWTRR852 pKa = 11.84RR853 pKa = 11.84TRR855 pKa = 11.84RR856 pKa = 11.84WSSSWRR862 pKa = 11.84SGRR865 pKa = 11.84PAPPAWTPSAATCLSALRR883 pKa = 11.84GWRR886 pKa = 11.84VSTRR890 pKa = 11.84SWARR894 pKa = 11.84HH895 pKa = 4.03MPSWTRR901 pKa = 11.84RR902 pKa = 11.84TRR904 pKa = 11.84RR905 pKa = 11.84WSSSWRR911 pKa = 11.84SGRR914 pKa = 11.84PAPPAWMPSAATCLSALHH932 pKa = 6.46GWRR935 pKa = 11.84VSTRR939 pKa = 11.84SWTRR943 pKa = 11.84RR944 pKa = 11.84TRR946 pKa = 11.84RR947 pKa = 11.84WSSSWRR953 pKa = 11.84SGRR956 pKa = 11.84PAPPAWMPSAATCLSALHH974 pKa = 6.46GWRR977 pKa = 11.84VSTRR981 pKa = 11.84SWARR985 pKa = 11.84HH986 pKa = 4.03MPSWTRR992 pKa = 11.84RR993 pKa = 11.84TRR995 pKa = 11.84RR996 pKa = 11.84WSSSWRR1002 pKa = 11.84SGRR1005 pKa = 11.84PAPPAWMPSAATCLSALHH1023 pKa = 6.46GWRR1026 pKa = 11.84VSTRR1030 pKa = 11.84SWARR1034 pKa = 11.84HH1035 pKa = 4.03MPSWTRR1041 pKa = 11.84RR1042 pKa = 11.84TRR1044 pKa = 11.84RR1045 pKa = 11.84WSSSWRR1051 pKa = 11.84SGRR1054 pKa = 11.84PAPPAWMPSAATCLSALRR1072 pKa = 11.84GWRR1075 pKa = 11.84VSTRR1079 pKa = 11.84SWARR1083 pKa = 11.84HH1084 pKa = 4.03MPSWTRR1090 pKa = 11.84RR1091 pKa = 11.84TRR1093 pKa = 11.84RR1094 pKa = 11.84WSSSWRR1100 pKa = 11.84SGRR1103 pKa = 11.84PAPPAWMPSAATCLSALHH1121 pKa = 6.46GWRR1124 pKa = 11.84VSTRR1128 pKa = 11.84SWARR1132 pKa = 11.84HH1133 pKa = 4.03MPSWTRR1139 pKa = 11.84RR1140 pKa = 11.84TRR1142 pKa = 11.84RR1143 pKa = 11.84WSSSWRR1149 pKa = 11.84SGRR1152 pKa = 11.84PAPPAWMPSAATCLSALRR1170 pKa = 11.84GWRR1173 pKa = 11.84VSTRR1177 pKa = 11.84SWARR1181 pKa = 11.84HH1182 pKa = 4.03MPSWTRR1188 pKa = 11.84RR1189 pKa = 11.84TRR1191 pKa = 11.84RR1192 pKa = 11.84WSSSWRR1198 pKa = 11.84SGRR1201 pKa = 11.84PAPPAWMPSAATCLSALRR1219 pKa = 11.84GWRR1222 pKa = 11.84VSTRR1226 pKa = 11.84SWTRR1230 pKa = 11.84RR1231 pKa = 11.84TRR1233 pKa = 11.84RR1234 pKa = 11.84WSSSWRR1240 pKa = 11.84SGRR1243 pKa = 11.84PAPPAWMPSAATCLSALSGWRR1264 pKa = 11.84VSTRR1268 pKa = 11.84SWARR1272 pKa = 11.84HH1273 pKa = 4.03MPSWTRR1279 pKa = 11.84RR1280 pKa = 11.84TRR1282 pKa = 11.84RR1283 pKa = 11.84WSSSWRR1289 pKa = 11.84SGRR1292 pKa = 11.84PAPPAWMPSAATCLSALRR1310 pKa = 11.84GWRR1313 pKa = 11.84VSTRR1317 pKa = 11.84SWARR1321 pKa = 11.84HH1322 pKa = 4.03MPSWTRR1328 pKa = 11.84RR1329 pKa = 11.84TRR1331 pKa = 11.84RR1332 pKa = 11.84WSSSWRR1338 pKa = 11.84SGRR1341 pKa = 11.84PAPPAWTPSAATCLSALHH1359 pKa = 6.46GWRR1362 pKa = 11.84VSTRR1366 pKa = 11.84SWARR1370 pKa = 11.84HH1371 pKa = 4.03MPSWTRR1377 pKa = 11.84RR1378 pKa = 11.84TRR1380 pKa = 11.84RR1381 pKa = 11.84WSSSWRR1387 pKa = 11.84SGRR1390 pKa = 11.84PAPPAWMPSAATCLSALRR1408 pKa = 11.84GWRR1411 pKa = 11.84VSTRR1415 pKa = 11.84SWASLMQGG1423 pKa = 3.17

Molecular weight:
164.93 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8084

0

8084

5077572

14

6733

628.1

68.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.166 ± 0.042

1.903 ± 0.012

4.811 ± 0.017

6.03 ± 0.033

3.007 ± 0.021

6.303 ± 0.022

2.764 ± 0.013

3.152 ± 0.019

3.388 ± 0.023

9.42 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.011

2.768 ± 0.015

5.717 ± 0.031

4.154 ± 0.018

7.114 ± 0.024

8.91 ± 0.054

6.289 ± 0.024

7.25 ± 0.021

1.1 ± 0.009

2.464 ± 0.016

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski