Manacus vitellinus (golden-collared manakin)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12707 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A093PW65|A0A093PW65_9PASS Nodal modulator 1 (Fragment) OS=Manacus vitellinus OX=328815 GN=N305_09670 PE=4 SV=1
CC1 pKa = 6.93NVGWAGNGNVCGPDD15 pKa = 3.43TDD17 pKa = 4.93LDD19 pKa = 4.37GYY21 pKa = 9.94PDD23 pKa = 4.1EE24 pKa = 5.31PLPCIDD30 pKa = 4.25NNKK33 pKa = 9.35HH34 pKa = 6.21CKK36 pKa = 9.14QDD38 pKa = 3.44NCRR41 pKa = 11.84LTPNSGQEE49 pKa = 3.95DD50 pKa = 4.26ADD52 pKa = 3.62NDD54 pKa = 5.11GIGDD58 pKa = 3.82QCDD61 pKa = 3.57DD62 pKa = 4.44DD63 pKa = 6.86ADD65 pKa = 4.16GDD67 pKa = 4.28GVKK70 pKa = 10.36NVEE73 pKa = 4.29DD74 pKa = 3.69NCRR77 pKa = 11.84LFPNKK82 pKa = 9.91DD83 pKa = 3.46QQNSDD88 pKa = 3.11TDD90 pKa = 4.03SFGDD94 pKa = 4.2ACDD97 pKa = 3.62NCPNVPNNDD106 pKa = 3.4QRR108 pKa = 11.84DD109 pKa = 3.62TDD111 pKa = 4.31SNGEE115 pKa = 3.91GDD117 pKa = 4.42ACDD120 pKa = 3.98NDD122 pKa = 3.39IDD124 pKa = 5.15GDD126 pKa = 4.46GIPNMLDD133 pKa = 3.17NCPKK137 pKa = 10.27VPNPLQTDD145 pKa = 3.4RR146 pKa = 11.84DD147 pKa = 3.67EE148 pKa = 6.42DD149 pKa = 4.4GVGDD153 pKa = 4.27ACDD156 pKa = 3.66SCPEE160 pKa = 4.12MSNPTQTDD168 pKa = 3.06MDD170 pKa = 4.69SDD172 pKa = 4.47LVGDD176 pKa = 3.68ICDD179 pKa = 3.85TNEE182 pKa = 4.14DD183 pKa = 3.74SDD185 pKa = 4.93GDD187 pKa = 3.91GHH189 pKa = 7.38QDD191 pKa = 3.18TKK193 pKa = 11.57DD194 pKa = 3.32NCAEE198 pKa = 4.25IPNSSQLDD206 pKa = 3.58SDD208 pKa = 4.01NDD210 pKa = 4.11GLGDD214 pKa = 4.98DD215 pKa = 5.48CDD217 pKa = 4.94NDD219 pKa = 4.37DD220 pKa = 5.42DD221 pKa = 5.52NDD223 pKa = 5.09GIPDD227 pKa = 4.02YY228 pKa = 11.2VPPGPDD234 pKa = 2.98NCRR237 pKa = 11.84LIPNPNQKK245 pKa = 10.66DD246 pKa = 3.36SDD248 pKa = 4.17GNGVGDD254 pKa = 3.6VCEE257 pKa = 4.36EE258 pKa = 4.28DD259 pKa = 3.68FDD261 pKa = 5.16NDD263 pKa = 4.06TVVDD267 pKa = 3.93QLDD270 pKa = 3.73VCPEE274 pKa = 3.98SAEE277 pKa = 4.05VTLTDD282 pKa = 3.82FRR284 pKa = 11.84AYY286 pKa = 8.36QTVILDD292 pKa = 3.91PEE294 pKa = 5.2GDD296 pKa = 3.69AQIDD300 pKa = 4.13PNWVVLNQGMEE311 pKa = 4.11IVQTMNSDD319 pKa = 3.19PGLAVGYY326 pKa = 7.31TAFNGVDD333 pKa = 3.99FEE335 pKa = 4.97GTFHH339 pKa = 6.81VNTVTDD345 pKa = 3.57DD346 pKa = 4.08DD347 pKa = 4.34YY348 pKa = 12.06AGFIFSYY355 pKa = 10.41QDD357 pKa = 2.92SASFYY362 pKa = 10.36VVMWKK367 pKa = 7.78QTEE370 pKa = 3.86QTYY373 pKa = 8.13WQATPFRR380 pKa = 11.84AVAEE384 pKa = 4.5PGLQLKK390 pKa = 9.47AVKK393 pKa = 10.34SSTGPGEE400 pKa = 4.21HH401 pKa = 6.64LRR403 pKa = 11.84NALWHH408 pKa = 6.26TGHH411 pKa = 6.51TPDD414 pKa = 5.16HH415 pKa = 6.71VRR417 pKa = 11.84LLWKK421 pKa = 10.36DD422 pKa = 3.2PRR424 pKa = 11.84NVGWRR429 pKa = 11.84DD430 pKa = 3.08KK431 pKa = 11.01TSYY434 pKa = 10.07RR435 pKa = 11.84WQLAHH440 pKa = 7.17RR441 pKa = 11.84PQVGYY446 pKa = 10.38IRR448 pKa = 4.61

Molecular weight: 49.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A093QBZ4|A0A093QBZ4_9PASS Zinc transporter 5 (Fragment) OS=Manacus vitellinus OX=328815 GN=N305_14703 PE=3 SV=1
GG1 pKa = 7.22KK2 pKa = 10.54GRR4 pKa = 11.84FGKK7 pKa = 10.68GRR9 pKa = 11.84FGKK12 pKa = 10.65GRR14 pKa = 11.84FGKK17 pKa = 10.65GRR19 pKa = 11.84FGKK22 pKa = 10.65GRR24 pKa = 11.84FGKK27 pKa = 10.65GRR29 pKa = 11.84FGKK32 pKa = 10.65GRR34 pKa = 11.84FGKK37 pKa = 10.65GRR39 pKa = 11.84FGKK42 pKa = 10.65GRR44 pKa = 11.84FGKK47 pKa = 10.65GRR49 pKa = 11.84FGKK52 pKa = 10.65GRR54 pKa = 11.84FGKK57 pKa = 10.7GRR59 pKa = 11.84FGG61 pKa = 3.6

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12707

0

12707

5377145

31

6219

423.2

47.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.43 ± 0.018

2.273 ± 0.021

5.026 ± 0.016

7.204 ± 0.03

3.92 ± 0.017

5.955 ± 0.027

2.57 ± 0.011

4.915 ± 0.018

6.367 ± 0.026

9.758 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.213 ± 0.008

4.058 ± 0.013

5.352 ± 0.024

4.676 ± 0.02

5.28 ± 0.018

8.144 ± 0.025

5.354 ± 0.015

6.325 ± 0.016

1.209 ± 0.008

2.968 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski