Camel associated porprismacovirus 3
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A1EL62|A0A0A1EL62_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 3 OX=2170107 GN=rep PE=4 SV=1
MM1 pKa = 7.45 QITSVACIPLSEE13 pKa = 4.42 GGKK16 pKa = 9.48 IATNYY21 pKa = 7.24 VTATYY26 pKa = 10.58 QEE28 pKa = 4.52 IYY30 pKa = 10.74 DD31 pKa = 3.93 LNTRR35 pKa = 11.84 EE36 pKa = 4.45 GKK38 pKa = 10.57 LSIIGIHH45 pKa = 5.95 TPTNIRR51 pKa = 11.84 PIAQLQGFFAQYY63 pKa = 10.99 KK64 pKa = 7.67 KK65 pKa = 10.71 FRR67 pKa = 11.84 YY68 pKa = 9.73 NGISKK73 pKa = 10.19 IAIQPAAQLPADD85 pKa = 4.13 PLQVSMEE92 pKa = 4.26 AGQTLDD98 pKa = 4.89 PRR100 pKa = 11.84 DD101 pKa = 4.06 LLNPILFHH109 pKa = 6.42 GAHH112 pKa = 5.68 GTSINAALNVAYY124 pKa = 10.12 RR125 pKa = 11.84 RR126 pKa = 11.84 TGHH129 pKa = 5.8 TFNTTSTNRR138 pKa = 11.84 EE139 pKa = 3.98 DD140 pKa = 3.06 VAAIYY145 pKa = 10.24 DD146 pKa = 3.71 FSGDD150 pKa = 3.65 EE151 pKa = 4.24 TPVARR156 pKa = 11.84 NLYY159 pKa = 9.73 YY160 pKa = 10.51 SALSDD165 pKa = 3.47 PSFTKK170 pKa = 10.5 FSPQQFIQLTDD181 pKa = 4.03 LVPMVHH187 pKa = 6.69 RR188 pKa = 11.84 VNTSMYY194 pKa = 9.78 FGPNQISEE202 pKa = 4.36 MAGSNNGSAGTAINTEE218 pKa = 3.89 IGRR221 pKa = 11.84 ASQGTVWNGNQEE233 pKa = 4.19 VTGPEE238 pKa = 4.1 AIKK241 pKa = 11.0 NEE243 pKa = 3.85 WTLGFQQFFSNGMAPLDD260 pKa = 3.61 WMPTRR265 pKa = 11.84 TFNNMGDD272 pKa = 3.69 VISTNEE278 pKa = 3.64 NRR280 pKa = 11.84 TNTEE284 pKa = 3.82 MFTEE288 pKa = 4.75 LPKK291 pKa = 10.88 LYY293 pKa = 9.47 MGVLIFPPSYY303 pKa = 9.86 NQKK306 pKa = 10.35 LYY308 pKa = 9.72 FRR310 pKa = 11.84 MVITHH315 pKa = 6.4 SFSFKK320 pKa = 10.99 DD321 pKa = 3.89 FGTYY325 pKa = 8.44 WSPEE329 pKa = 3.79 MGDD332 pKa = 3.23 WGLLEE337 pKa = 4.31 YY338 pKa = 10.71 FNEE341 pKa = 4.31 FDD343 pKa = 5.0 SSSTTQASSLNLTEE357 pKa = 4.83 ADD359 pKa = 3.49 AVLEE363 pKa = 4.11 NDD365 pKa = 3.71 GVMM368 pKa = 4.34
Molecular weight: 40.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.81
IPC2_protein 4.902
IPC_protein 4.838
Toseland 4.736
ProMoST 4.952
Dawson 4.8
Bjellqvist 4.94
Wikipedia 4.673
Rodwell 4.711
Grimsley 4.647
Solomon 4.8
Lehninger 4.762
Nozaki 4.914
DTASelect 5.067
Thurlkill 4.724
EMBOSS 4.698
Sillero 4.978
Patrickios 3.656
IPC_peptide 4.813
IPC2_peptide 4.978
IPC2.peptide.svr19 4.931
Protein with the highest isoelectric point:
>tr|A0A0A1EL62|A0A0A1EL62_9VIRU Putative replicase protein OS=Camel associated porprismacovirus 3 OX=2170107 GN=rep PE=4 SV=1
MM1 pKa = 6.53 VTVPTKK7 pKa = 10.15 HH8 pKa = 5.09 ACKK11 pKa = 9.91 RR12 pKa = 11.84 QVKK15 pKa = 9.19 MILEE19 pKa = 4.17 IFDD22 pKa = 3.42 TKK24 pKa = 10.72 RR25 pKa = 11.84 YY26 pKa = 9.89 IFAQEE31 pKa = 3.73 KK32 pKa = 9.5 GKK34 pKa = 10.55 NGLDD38 pKa = 2.97 HH39 pKa = 6.29 WQLRR43 pKa = 11.84 IQISGDD49 pKa = 3.05 KK50 pKa = 10.49 EE51 pKa = 4.23 EE52 pKa = 4.32 NFEE55 pKa = 4.09 RR56 pKa = 11.84 LQKK59 pKa = 10.52 LFCNKK64 pKa = 9.31 AHH66 pKa = 6.87 IEE68 pKa = 4.16 VANDD72 pKa = 2.45 SWEE75 pKa = 4.04 YY76 pKa = 9.95 EE77 pKa = 4.04 RR78 pKa = 11.84 KK79 pKa = 9.67 EE80 pKa = 3.98 GKK82 pKa = 9.22 FWSSEE87 pKa = 3.87 DD88 pKa = 3.24 TKK90 pKa = 11.07 EE91 pKa = 3.62 ILKK94 pKa = 10.16 IRR96 pKa = 11.84 FGKK99 pKa = 10.03 LRR101 pKa = 11.84 SEE103 pKa = 4.2 QKK105 pKa = 10.57 KK106 pKa = 9.68 ILQILSSQGDD116 pKa = 3.59 RR117 pKa = 11.84 EE118 pKa = 3.93 IDD120 pKa = 3.23 VWLDD124 pKa = 3.08 PTGCHH129 pKa = 5.5 GKK131 pKa = 9.28 SWLTIHH137 pKa = 6.61 LWEE140 pKa = 4.39 TGRR143 pKa = 11.84 ALVVPRR149 pKa = 11.84 SSTTAEE155 pKa = 3.92 KK156 pKa = 10.77 LSAFVCSSWRR166 pKa = 11.84 GEE168 pKa = 4.18 DD169 pKa = 3.31 IIVIDD174 pKa = 4.07 VPRR177 pKa = 11.84 ATKK180 pKa = 8.66 PTTSLLEE187 pKa = 4.11 TMEE190 pKa = 4.13 EE191 pKa = 4.16 LKK193 pKa = 11.09 DD194 pKa = 3.59 GLVFDD199 pKa = 4.61 HH200 pKa = 7.49 RR201 pKa = 11.84 YY202 pKa = 7.34 TGKK205 pKa = 8.03 TRR207 pKa = 11.84 NVRR210 pKa = 11.84 GTKK213 pKa = 10.57 LMVFTNSPLPLNKK226 pKa = 10.16 LSTDD230 pKa = 2.59 RR231 pKa = 11.84 WRR233 pKa = 11.84 LHH235 pKa = 6.81 GISADD240 pKa = 3.62 GSLSS244 pKa = 3.34
Molecular weight: 28.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.681
IPC2_protein 8.375
IPC_protein 8.302
Toseland 9.238
ProMoST 8.887
Dawson 9.428
Bjellqvist 9.194
Wikipedia 9.502
Rodwell 9.882
Grimsley 9.399
Solomon 9.516
Lehninger 9.502
Nozaki 9.502
DTASelect 9.092
Thurlkill 9.341
EMBOSS 9.648
Sillero 9.458
Patrickios 7.658
IPC_peptide 9.516
IPC2_peptide 7.775
IPC2.peptide.svr19 7.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
612
244
368
306.0
34.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.882 ± 1.21
0.817 ± 0.453
4.902 ± 0.461
6.209 ± 0.87
5.065 ± 0.759
6.863 ± 0.394
2.124 ± 0.411
6.373 ± 0.102
5.392 ± 2.224
8.17 ± 0.919
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.778 ± 0.628
5.719 ± 1.571
4.412 ± 0.851
4.412 ± 0.625
4.902 ± 1.139
7.68 ± 0.059
8.497 ± 0.617
4.739 ± 0.325
2.124 ± 0.637
2.941 ± 0.944
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here