Mesorhizobium sp. MaA-C15

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Mesorhizobium

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4601 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5D4GXC9|A0A5D4GXC9_9RHIZ LacI family DNA-binding transcriptional regulator OS=Mesorhizobium sp. MaA-C15 OX=2682781 GN=FY036_08375 PE=4 SV=1
MM1 pKa = 7.34SKK3 pKa = 10.29FVRR6 pKa = 11.84ILAFVCLAAFAAGTAANAAASTEE29 pKa = 4.02MSLMMSMSSMDD40 pKa = 4.09DD41 pKa = 3.19GGMADD46 pKa = 4.63CQDD49 pKa = 3.93CPGDD53 pKa = 3.82DD54 pKa = 4.0SQASEE59 pKa = 4.46CDD61 pKa = 3.56QFCVTSLAAICTPAGTDD78 pKa = 3.65LPNVTDD84 pKa = 3.65VVVIGTPTPTSDD96 pKa = 4.5LSGPPDD102 pKa = 3.62LHH104 pKa = 6.5PPRR107 pKa = 11.84TIRR110 pKa = 11.84LGG112 pKa = 3.43

Molecular weight: 11.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5D4GL30|A0A5D4GL30_9RHIZ DUF1269 domain-containing protein OS=Mesorhizobium sp. MaA-C15 OX=2682781 GN=FY036_22065 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.25GGRR28 pKa = 11.84GVIAARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4601

0

4601

1442372

36

2842

313.5

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.645 ± 0.052

0.785 ± 0.01

5.671 ± 0.029

6.09 ± 0.033

3.863 ± 0.024

8.678 ± 0.029

2.027 ± 0.018

5.317 ± 0.028

3.07 ± 0.028

9.875 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.702 ± 0.015

2.527 ± 0.018

4.945 ± 0.025

2.955 ± 0.022

7.218 ± 0.037

5.334 ± 0.024

5.16 ± 0.021

7.62 ± 0.031

1.312 ± 0.015

2.208 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski