His 2 virus (His2V) (Haloarcula hispanica virus 2)

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Gammapleolipovirus; Gammapleolipovirus His2

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q25BD3|Q25BD3_HIS2V Uncharacterized protein OS=His 2 virus OX=128710 PE=4 SV=1
MM1 pKa = 7.65HH2 pKa = 7.58YY3 pKa = 10.24LRR5 pKa = 11.84KK6 pKa = 8.88KK7 pKa = 9.28TITTTNTMNIKK18 pKa = 9.88KK19 pKa = 10.26AFVGVMLALTVISSMAMPAAAAGTLYY45 pKa = 10.96VGTSEE50 pKa = 4.41TNDD53 pKa = 2.83AVTYY57 pKa = 9.06STVDD61 pKa = 3.37DD62 pKa = 4.36AVANASDD69 pKa = 3.78GDD71 pKa = 4.33TIEE74 pKa = 4.85LAPGTYY80 pKa = 9.64NASIEE85 pKa = 4.37VNTANLTIQRR95 pKa = 11.84DD96 pKa = 3.87SSVSSGTVTIDD107 pKa = 3.68ASGEE111 pKa = 4.09KK112 pKa = 10.62YY113 pKa = 10.71GDD115 pKa = 3.34AFYY118 pKa = 11.03GSNDD122 pKa = 3.05SSLSLGTNVAVTEE135 pKa = 4.15NVVTVDD141 pKa = 4.01SANTGNADD149 pKa = 3.59TYY151 pKa = 10.79GTISDD156 pKa = 4.35AMDD159 pKa = 3.31NTTANSTISIAPGTYY174 pKa = 7.97NTSINVTSDD183 pKa = 2.66NVGFVNNAPASGNVTIDD200 pKa = 3.24ATNTTEE206 pKa = 3.88GSAFVGNYY214 pKa = 9.25SDD216 pKa = 3.61NVRR219 pKa = 11.84VGGDD223 pKa = 3.04ITVEE227 pKa = 3.96EE228 pKa = 4.52SVLGIGGGQFGEE240 pKa = 4.55SVGNALGANVFGIPFWAIGVVALLVGYY267 pKa = 9.99IYY269 pKa = 9.4QTEE272 pKa = 3.89AA273 pKa = 3.22

Molecular weight: 27.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q25BF0|Q25BF0_HIS2V Uncharacterized protein OS=His 2 virus OX=128710 PE=4 SV=1
MM1 pKa = 7.46KK2 pKa = 10.28KK3 pKa = 8.67VTILILKK10 pKa = 10.29ASLAPLYY17 pKa = 9.55MATCLKK23 pKa = 10.82NPAA26 pKa = 4.08

Molecular weight: 2.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

4584

26

720

131.0

14.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.523 ± 0.484

1.636 ± 0.496

6.021 ± 0.485

8.421 ± 0.758

3.294 ± 0.314

6.283 ± 0.47

1.745 ± 0.333

6.894 ± 0.379

6.108 ± 0.697

6.697 ± 0.509

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.291

6.043 ± 0.363

2.771 ± 0.264

3.905 ± 0.38

3.73 ± 0.405

6.501 ± 0.548

7.504 ± 0.631

7.286 ± 0.736

0.916 ± 0.107

4.516 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski