Puniceibacterium antarcticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Puniceibacterium

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G8R816|A0A2G8R816_9RHOB MPN domain-containing protein OS=Puniceibacterium antarcticum OX=1206336 GN=P775_23625 PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 9.05RR3 pKa = 11.84TLVLSTALVGAMTLPAFAGSLVPATVEE30 pKa = 3.97PAPMAPAPVAAPMMASADD48 pKa = 3.28WTGFSLGAQAGYY60 pKa = 10.4GAFDD64 pKa = 4.37SEE66 pKa = 5.45NPDD69 pKa = 4.1DD70 pKa = 6.2DD71 pKa = 6.37DD72 pKa = 5.35DD73 pKa = 6.32AGTYY77 pKa = 8.76GLKK80 pKa = 10.39GGYY83 pKa = 9.85DD84 pKa = 4.04FDD86 pKa = 3.69MGSYY90 pKa = 10.68VLGLGMQYY98 pKa = 11.21DD99 pKa = 4.05ATDD102 pKa = 3.26VDD104 pKa = 5.01LGDD107 pKa = 4.49ANVDD111 pKa = 3.79GILRR115 pKa = 11.84AGGRR119 pKa = 11.84IGYY122 pKa = 9.31DD123 pKa = 2.95LGKK126 pKa = 8.3TLPYY130 pKa = 9.94FAAGYY135 pKa = 10.45AKK137 pKa = 10.68AFTDD141 pKa = 4.0DD142 pKa = 3.8SSVGDD147 pKa = 3.69SNGYY151 pKa = 8.61YY152 pKa = 10.32AGLGVEE158 pKa = 4.45RR159 pKa = 11.84MLTRR163 pKa = 11.84SVSLGGEE170 pKa = 3.82VLYY173 pKa = 11.37NKK175 pKa = 9.99FDD177 pKa = 4.2DD178 pKa = 4.3FDD180 pKa = 4.43NSFEE184 pKa = 4.24TDD186 pKa = 2.7ATTANVSVNYY196 pKa = 10.29RR197 pKa = 11.84FF198 pKa = 3.5

Molecular weight: 20.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G8RC10|A0A2G8RC10_9RHOB Uncharacterized protein OS=Puniceibacterium antarcticum OX=1206336 GN=P775_16640 PE=4 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.12RR4 pKa = 11.84TFQPSNLVRR13 pKa = 11.84KK14 pKa = 8.83HH15 pKa = 4.64RR16 pKa = 11.84HH17 pKa = 3.91GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37AGRR29 pKa = 11.84KK30 pKa = 8.54ILNARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.07SLSAA45 pKa = 3.93

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5603

0

5603

1590982

26

2394

284.0

30.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.066 ± 0.046

0.919 ± 0.011

6.013 ± 0.027

5.447 ± 0.029

3.718 ± 0.022

8.557 ± 0.03

2.054 ± 0.018

5.261 ± 0.023

3.154 ± 0.024

10.284 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.015

2.612 ± 0.017

4.994 ± 0.026

3.378 ± 0.019

6.649 ± 0.033

5.504 ± 0.025

5.677 ± 0.022

7.246 ± 0.031

1.385 ± 0.013

2.199 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski