Saitozyma podzolica
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10205 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A427XL15|A0A427XL15_9TREE MFS domain-containing protein OS=Saitozyma podzolica OX=1890683 GN=EHS25_007968 PE=4 SV=1
MM1 pKa = 7.21 ATVNSVSSMMSGMSSVSRR19 pKa = 11.84 PLPMHH24 pKa = 6.47 TAATPQPEE32 pKa = 4.39 APTLGLGLDD41 pKa = 4.04 LNLNPDD47 pKa = 3.34 VGEE50 pKa = 4.45 DD51 pKa = 3.28 TDD53 pKa = 4.36 GRR55 pKa = 11.84 SDD57 pKa = 3.86 PYY59 pKa = 10.36 MGSPSSARR67 pKa = 11.84 LQALNCSSSFLGFCMGSTITIPSTNTTLNPIFPEE101 pKa = 4.19 LNPDD105 pKa = 2.87 VDD107 pKa = 3.5 IYY109 pKa = 7.95 TARR112 pKa = 11.84 MEE114 pKa = 4.36 DD115 pKa = 3.01 EE116 pKa = 4.44 MMFSFSPQGVPGNSGDD132 pKa = 3.29 VDD134 pKa = 3.61 GFYY137 pKa = 10.34 DD138 pKa = 4.04 YY139 pKa = 11.24 PFTPVMSTLPLPLPALGSLGPSQVNTANEE168 pKa = 4.49 GTTMAATSQFLGDD181 pKa = 3.61 SS182 pKa = 3.7
Molecular weight: 19.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.656
IPC_protein 3.617
Toseland 3.401
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.745
Patrickios 0.769
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A427Y3E9|A0A427Y3E9_9TREE Uncharacterized protein OS=Saitozyma podzolica OX=1890683 GN=EHS25_003738 PE=4 SV=1
MM1 pKa = 7.31 SRR3 pKa = 11.84 SQVGEE8 pKa = 3.77 PLARR12 pKa = 11.84 ASPIGTYY19 pKa = 10.47 GPPCQSHH26 pKa = 6.36 SLSDD30 pKa = 3.89 QPPPLHH36 pKa = 6.78 PPPHH40 pKa = 6.59 LPPHH44 pKa = 6.29 VGLSGVHH51 pKa = 5.54 AHH53 pKa = 6.61 HH54 pKa = 7.19 PSSYY58 pKa = 8.88 KK59 pKa = 9.71 QSNGSVHH66 pKa = 6.86 AGHH69 pKa = 7.23 PHH71 pKa = 4.91 THH73 pKa = 7.19 ALPALPAPWRR83 pKa = 11.84 STCRR87 pKa = 11.84 TFPSPVAAGEE97 pKa = 4.04 MSPTTFKK104 pKa = 11.22 GGVAVKK110 pKa = 10.34 RR111 pKa = 11.84 GTAATKK117 pKa = 10.2 GSQRR121 pKa = 11.84 PARR124 pKa = 11.84 DD125 pKa = 3.65 HH126 pKa = 6.69 EE127 pKa = 4.85 SGSEE131 pKa = 3.95 DD132 pKa = 3.55 EE133 pKa = 4.63 DD134 pKa = 3.88 KK135 pKa = 11.06 KK136 pKa = 11.45 GKK138 pKa = 10.33 SKK140 pKa = 11.29 LEE142 pKa = 3.57 NRR144 pKa = 11.84 RR145 pKa = 11.84 EE146 pKa = 4.07 KK147 pKa = 11.16 NRR149 pKa = 11.84 VKK151 pKa = 10.3 QRR153 pKa = 11.84 KK154 pKa = 8.5 LRR156 pKa = 11.84 MRR158 pKa = 11.84 RR159 pKa = 11.84 ANHH162 pKa = 5.29 IVEE165 pKa = 5.0 LEE167 pKa = 3.98 TSITSLRR174 pKa = 11.84 SEE176 pKa = 4.2 HH177 pKa = 6.49 TSLQSAFSVSQLRR190 pKa = 11.84 EE191 pKa = 3.54 AHH193 pKa = 6.04 LEE195 pKa = 3.83 SRR197 pKa = 11.84 IRR199 pKa = 11.84 DD200 pKa = 3.38 LGLALFSDD208 pKa = 4.68 GLSGEE213 pKa = 4.06 VDD215 pKa = 3.0 ATRR218 pKa = 11.84 RR219 pKa = 11.84 AWSVVSSSSTPTGPLGCEE237 pKa = 4.19 GGGKK241 pKa = 9.6 RR242 pKa = 11.84 PRR244 pKa = 11.84 TISGRR249 pKa = 11.84 DD250 pKa = 3.52 PLNTLAQAALPLEE263 pKa = 4.58 HH264 pKa = 6.86 ARR266 pKa = 11.84 EE267 pKa = 4.35 GATLPPLVSQWNRR280 pKa = 11.84 PSSRR284 pKa = 11.84 HH285 pKa = 5.57 SEE287 pKa = 3.98 SDD289 pKa = 2.95 EE290 pKa = 4.2 KK291 pKa = 10.88 KK292 pKa = 10.42 CKK294 pKa = 10.2 RR295 pKa = 11.84 EE296 pKa = 3.95 ALDD299 pKa = 3.75 APLYY303 pKa = 10.16 PPTFGKK309 pKa = 10.72 SSTSRR314 pKa = 11.84 GYY316 pKa = 10.71 SRR318 pKa = 11.84 TRR320 pKa = 11.84 EE321 pKa = 3.81 HH322 pKa = 6.38 SPRR325 pKa = 11.84 RR326 pKa = 11.84 IPVEE330 pKa = 3.71 PTTAGASSAGISEE343 pKa = 4.37 PLVNPSLRR351 pKa = 11.84 SSRR354 pKa = 11.84 VASAPLPPLAPSSSSNSLFTTPEE377 pKa = 3.39 WAAPSPGFIRR387 pKa = 11.84 IEE389 pKa = 4.18 DD390 pKa = 4.3 LLSLMGPAAASLRR403 pKa = 11.84 SQAHH407 pKa = 4.63 TPKK410 pKa = 10.51 KK411 pKa = 9.54 RR412 pKa = 11.84 DD413 pKa = 3.33 QEE415 pKa = 4.17 RR416 pKa = 11.84 EE417 pKa = 3.76 RR418 pKa = 11.84 EE419 pKa = 4.54 GISDD423 pKa = 3.56 EE424 pKa = 4.04 AVLRR428 pKa = 11.84 RR429 pKa = 11.84 RR430 pKa = 11.84 GFGGRR435 pKa = 11.84 PVPRR439 pKa = 11.84 GRR441 pKa = 11.84 PRR443 pKa = 11.84 EE444 pKa = 4.25 SRR446 pKa = 11.84 CHH448 pKa = 6.04 ASSCGRR454 pKa = 11.84 CRR456 pKa = 11.84 PRR458 pKa = 11.84 FAVPVIIPPPDD469 pKa = 3.8 PPVSLLLCPHH479 pKa = 6.96 RR480 pKa = 11.84 PLAPAALVPFSLAIPFATPLAPDD503 pKa = 3.8 TANHH507 pKa = 6.25 SKK509 pKa = 10.57 LALSRR514 pKa = 11.84 CPTSPHH520 pKa = 6.44 HH521 pKa = 6.52 LLPSTTRR528 pKa = 11.84 PAAPQPLTRR537 pKa = 11.84 RR538 pKa = 11.84 RR539 pKa = 11.84 KK540 pKa = 4.89 THH542 pKa = 5.52 RR543 pKa = 11.84 RR544 pKa = 11.84 RR545 pKa = 11.84 PILLFRR551 pKa = 11.84 RR552 pKa = 11.84 RR553 pKa = 11.84 ASRR556 pKa = 11.84 ALSHH560 pKa = 6.31 HH561 pKa = 6.45 HH562 pKa = 6.89 PAGQGHH568 pKa = 7.16 PAPPQRR574 pKa = 11.84 NADD577 pKa = 3.54 SCLEE581 pKa = 4.69 LISHH585 pKa = 6.71 TLASVRR591 pKa = 11.84 EE592 pKa = 4.42 SGDD595 pKa = 3.49 PPDD598 pKa = 4.26 AEE600 pKa = 3.97 AWEE603 pKa = 4.47 LQDD606 pKa = 5.55 SYY608 pKa = 11.24 WDD610 pKa = 3.33 TARR613 pKa = 11.84 ASFRR617 pKa = 11.84 IPASHH622 pKa = 6.88 HH623 pKa = 5.92 SFSILSQSEE632 pKa = 3.91 HH633 pKa = 6.21 AGARR637 pKa = 11.84 LHH639 pKa = 6.55 ALSSALFSRR648 pKa = 11.84 VPRR651 pKa = 11.84 TNLTRR656 pKa = 11.84 SPFVDD661 pKa = 3.62 LRR663 pKa = 11.84 RR664 pKa = 11.84 RR665 pKa = 11.84 LSLRR669 pKa = 11.84 YY670 pKa = 8.95 IAIGSRR676 pKa = 11.84 PRR678 pKa = 11.84 HH679 pKa = 4.7 RR680 pKa = 11.84 TEE682 pKa = 4.76 ALASTPGTRR691 pKa = 11.84 VRR693 pKa = 11.84 LEE695 pKa = 3.99 PLGHH699 pKa = 4.73 QTSRR703 pKa = 11.84 RR704 pKa = 11.84 NDD706 pKa = 3.11 IQVITLLGSQATGLANAEE724 pKa = 3.85 YY725 pKa = 10.46 DD726 pKa = 3.76 LTVVSLASKK735 pKa = 9.49 DD736 pKa = 3.59 ACSMSLPDD744 pKa = 4.52 QDD746 pKa = 4.75 GNPPRR751 pKa = 11.84 LVNKK755 pKa = 10.21 YY756 pKa = 9.06 LDD758 pKa = 3.63 SVADD762 pKa = 3.41 HH763 pKa = 5.78 TTRR766 pKa = 11.84 SGTAQPAISRR776 pKa = 11.84 STHH779 pKa = 5.61 CLLPRR784 pKa = 11.84 RR785 pKa = 11.84 HH786 pKa = 7.07 DD787 pKa = 3.62 EE788 pKa = 4.27 RR789 pKa = 11.84 QHH791 pKa = 5.99 HH792 pKa = 6.36 QGLCLLEE799 pKa = 4.45 TGNDD803 pKa = 3.14 TWNIQPDD810 pKa = 4.12 GQTVEE815 pKa = 4.3 PLPFAGEE822 pKa = 4.68 GPQLL826 pKa = 3.61
Molecular weight: 89.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.399
IPC_protein 10.218
Toseland 10.628
ProMoST 10.365
Dawson 10.701
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 10.774
Grimsley 10.745
Solomon 10.862
Lehninger 10.818
Nozaki 10.643
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.038
Sillero 10.643
Patrickios 10.452
IPC_peptide 10.862
IPC2_peptide 9.677
IPC2.peptide.svr19 8.687
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10205
0
10205
5014707
50
4841
491.4
53.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.624 ± 0.026
1.044 ± 0.008
5.508 ± 0.018
6.08 ± 0.022
3.34 ± 0.015
7.84 ± 0.021
2.393 ± 0.011
4.247 ± 0.015
4.057 ± 0.02
8.982 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.114 ± 0.01
2.879 ± 0.011
6.95 ± 0.034
3.535 ± 0.019
6.442 ± 0.022
8.728 ± 0.031
5.958 ± 0.017
6.415 ± 0.016
1.487 ± 0.01
2.376 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here