Sodalis phage phiSG1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A3E2I5|A3E2I5_9CAUD Gp23 OS=Sodalis phage phiSG1 OX=373126 PE=4 SV=1
MM1 pKa = 7.72PKK3 pKa = 10.27DD4 pKa = 3.49ISLDD8 pKa = 4.05DD9 pKa = 3.59IKK11 pKa = 11.1EE12 pKa = 3.95IALRR16 pKa = 11.84AYY18 pKa = 10.17RR19 pKa = 11.84GGEE22 pKa = 3.84VRR24 pKa = 11.84SAYY27 pKa = 9.99VDD29 pKa = 3.46KK30 pKa = 11.45SDD32 pKa = 4.22DD33 pKa = 3.4NSLISEE39 pKa = 4.84EE40 pKa = 4.18KK41 pKa = 10.06TEE43 pKa = 4.38LPPEE47 pKa = 4.41SVDD50 pKa = 3.68NPEE53 pKa = 3.86EE54 pKa = 3.91NAPEE58 pKa = 4.03SEE60 pKa = 4.33EE61 pKa = 3.98TVEE64 pKa = 4.08AVEE67 pKa = 5.09DD68 pKa = 3.8ATTVTDD74 pKa = 5.63SPDD77 pKa = 3.76CDD79 pKa = 3.86TQSSEE84 pKa = 4.6SEE86 pKa = 3.94EE87 pKa = 4.12TKK89 pKa = 11.05ARR91 pKa = 11.84DD92 pKa = 3.54WPDD95 pKa = 3.27LEE97 pKa = 5.78LDD99 pKa = 3.12IARR102 pKa = 11.84ALWEE106 pKa = 4.98GEE108 pKa = 4.23LHH110 pKa = 6.23WEE112 pKa = 5.11FIPSEE117 pKa = 4.15VEE119 pKa = 3.26QWARR123 pKa = 11.84KK124 pKa = 6.06MVKK127 pKa = 9.99RR128 pKa = 11.84RR129 pKa = 11.84DD130 pKa = 3.53GEE132 pKa = 4.07MMRR135 pKa = 11.84WLLVLQQVPDD145 pKa = 3.17ILSRR149 pKa = 11.84SRR151 pKa = 11.84EE152 pKa = 3.99FIFKK156 pKa = 10.1VIRR159 pKa = 11.84SPHH162 pKa = 6.26DD163 pKa = 3.26PEE165 pKa = 6.47IYY167 pKa = 8.87LTPEE171 pKa = 4.66AIDD174 pKa = 4.22AYY176 pKa = 9.96VASFVEE182 pKa = 4.2QEE184 pKa = 4.41TVEE187 pKa = 4.07EE188 pKa = 4.61VPWSEE193 pKa = 4.43EE194 pKa = 3.49ATADD198 pKa = 3.28IAEE201 pKa = 4.37TLTLEE206 pKa = 4.8RR207 pKa = 11.84DD208 pKa = 3.57AQRR211 pKa = 11.84LAKK214 pKa = 10.04FLEE217 pKa = 4.68PEE219 pKa = 4.17AVDD222 pKa = 4.66LPEE225 pKa = 6.35GPDD228 pKa = 4.9ADD230 pKa = 4.86DD231 pKa = 3.36EE232 pKa = 4.55WVPGVPEE239 pKa = 4.68GEE241 pKa = 4.34PEE243 pKa = 4.14TTVEE247 pKa = 4.91SEE249 pKa = 4.07TADD252 pKa = 3.47APDD255 pKa = 3.17IAEE258 pKa = 4.67PEE260 pKa = 4.42TVDD263 pKa = 4.52AEE265 pKa = 4.28ATEE268 pKa = 4.25SEE270 pKa = 4.65IYY272 pKa = 10.52YY273 pKa = 10.55SPEE276 pKa = 3.25TDD278 pKa = 2.95MPTFLRR284 pKa = 11.84DD285 pKa = 3.41LTPVNGAEE293 pKa = 3.81EE294 pKa = 4.45DD295 pKa = 3.59GRR297 pKa = 11.84LFATVDD303 pKa = 3.39ALNEE307 pKa = 3.97RR308 pKa = 11.84LAGIPAGGSLVLWGLDD324 pKa = 2.87NGLNHH329 pKa = 6.57SADD332 pKa = 4.5GYY334 pKa = 10.77SSTQIRR340 pKa = 11.84IALRR344 pKa = 11.84RR345 pKa = 11.84GKK347 pKa = 9.32PAATYY352 pKa = 8.87WYY354 pKa = 9.2KK355 pKa = 10.71DD356 pKa = 3.12APRR359 pKa = 11.84RR360 pKa = 11.84QEE362 pKa = 3.58DD363 pKa = 3.3SRR365 pKa = 11.84ALFIGSAVHH374 pKa = 6.81AALLEE379 pKa = 4.1PEE381 pKa = 4.47RR382 pKa = 11.84FATEE386 pKa = 4.02YY387 pKa = 11.31ACAPSVNLRR396 pKa = 11.84TSEE399 pKa = 4.01GKK401 pKa = 8.05EE402 pKa = 3.66TLASFEE408 pKa = 4.51CDD410 pKa = 2.88CATRR414 pKa = 11.84NLIPIKK420 pKa = 10.58RR421 pKa = 11.84EE422 pKa = 3.97DD423 pKa = 3.9FDD425 pKa = 4.7TVCMMRR431 pKa = 11.84DD432 pKa = 3.26SVLAFPVVRR441 pKa = 11.84LLLSSGVGEE450 pKa = 4.72LSVFWRR456 pKa = 11.84MEE458 pKa = 3.64NGLLLKK464 pKa = 10.12IRR466 pKa = 11.84PDD468 pKa = 3.1WLGVYY473 pKa = 9.91QDD475 pKa = 4.44APFMLDD481 pKa = 3.52VKK483 pKa = 10.28TLDD486 pKa = 3.75DD487 pKa = 3.88VNDD490 pKa = 5.01FGRR493 pKa = 11.84QAHH496 pKa = 4.86EE497 pKa = 4.08HH498 pKa = 6.64GYY500 pKa = 10.09HH501 pKa = 5.8IQAAYY506 pKa = 9.87YY507 pKa = 10.23GFVLEE512 pKa = 5.15HH513 pKa = 5.99VFGMVINGLFCTIGKK528 pKa = 8.5RR529 pKa = 11.84TEE531 pKa = 3.86CGRR534 pKa = 11.84YY535 pKa = 8.5PVEE538 pKa = 5.12LGPMDD543 pKa = 4.43NGDD546 pKa = 3.92SEE548 pKa = 5.18EE549 pKa = 4.54GLLQVHH555 pKa = 6.86DD556 pKa = 5.72AITVLNGPDD565 pKa = 4.62DD566 pKa = 6.03DD567 pKa = 4.11IASCFTVFSRR577 pKa = 11.84PSWAKK582 pKa = 8.98QADD585 pKa = 3.9RR586 pKa = 11.84KK587 pKa = 10.3RR588 pKa = 11.84RR589 pKa = 11.84EE590 pKa = 4.1AQEE593 pKa = 3.91VMAA596 pKa = 6.07

Molecular weight:
66.75 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A3E2L4|A3E2L4_9CAUD Gp55 OS=Sodalis phage phiSG1 OX=373126 PE=3 SV=1
MM1 pKa = 7.53AMKK4 pKa = 10.3DD5 pKa = 2.88KK6 pKa = 10.99SYY8 pKa = 10.37AQRR11 pKa = 11.84RR12 pKa = 11.84FEE14 pKa = 3.76RR15 pKa = 11.84TRR17 pKa = 11.84AHH19 pKa = 6.63FEE21 pKa = 4.09KK22 pKa = 10.73INDD25 pKa = 3.52DD26 pKa = 3.34RR27 pKa = 11.84ALARR31 pKa = 11.84KK32 pKa = 9.08VSKK35 pKa = 10.26AWAKK39 pKa = 8.53ITQPEE44 pKa = 4.16QTLKK48 pKa = 10.78AYY50 pKa = 9.43RR51 pKa = 11.84QQIIDD56 pKa = 3.18ATEE59 pKa = 3.96RR60 pKa = 11.84YY61 pKa = 8.6NALKK65 pKa = 10.54KK66 pKa = 9.12SARR69 pKa = 11.84YY70 pKa = 9.69RR71 pKa = 11.84MAIARR76 pKa = 4.05

Molecular weight:
9.09 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12786

71

724

250.7

27.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.48 ± 0.688

1.22 ± 0.151

5.85 ± 0.328

6.796 ± 0.364

3.582 ± 0.22

7.164 ± 0.34

2.112 ± 0.21

5.279 ± 0.259

5.529 ± 0.331

8.376 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.464 ± 0.148

4.012 ± 0.283

4.106 ± 0.345

4.974 ± 0.409

6.499 ± 0.405

5.608 ± 0.254

5.545 ± 0.212

6.233 ± 0.271

1.251 ± 0.122

2.917 ± 0.234

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski