Sodalis phage phiSG1
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A3E2I5|A3E2I5_9CAUD Gp23 OS=Sodalis phage phiSG1 OX=373126 PE=4 SV=1
MM1 pKa = 7.72 PKK3 pKa = 10.27 DD4 pKa = 3.49 ISLDD8 pKa = 4.05 DD9 pKa = 3.59 IKK11 pKa = 11.1 EE12 pKa = 3.95 IALRR16 pKa = 11.84 AYY18 pKa = 10.17 RR19 pKa = 11.84 GGEE22 pKa = 3.84 VRR24 pKa = 11.84 SAYY27 pKa = 9.99 VDD29 pKa = 3.46 KK30 pKa = 11.45 SDD32 pKa = 4.22 DD33 pKa = 3.4 NSLISEE39 pKa = 4.84 EE40 pKa = 4.18 KK41 pKa = 10.06 TEE43 pKa = 4.38 LPPEE47 pKa = 4.41 SVDD50 pKa = 3.68 NPEE53 pKa = 3.86 EE54 pKa = 3.91 NAPEE58 pKa = 4.03 SEE60 pKa = 4.33 EE61 pKa = 3.98 TVEE64 pKa = 4.08 AVEE67 pKa = 5.09 DD68 pKa = 3.8 ATTVTDD74 pKa = 5.63 SPDD77 pKa = 3.76 CDD79 pKa = 3.86 TQSSEE84 pKa = 4.6 SEE86 pKa = 3.94 EE87 pKa = 4.12 TKK89 pKa = 11.05 ARR91 pKa = 11.84 DD92 pKa = 3.54 WPDD95 pKa = 3.27 LEE97 pKa = 5.78 LDD99 pKa = 3.12 IARR102 pKa = 11.84 ALWEE106 pKa = 4.98 GEE108 pKa = 4.23 LHH110 pKa = 6.23 WEE112 pKa = 5.11 FIPSEE117 pKa = 4.15 VEE119 pKa = 3.26 QWARR123 pKa = 11.84 KK124 pKa = 6.06 MVKK127 pKa = 9.99 RR128 pKa = 11.84 RR129 pKa = 11.84 DD130 pKa = 3.53 GEE132 pKa = 4.07 MMRR135 pKa = 11.84 WLLVLQQVPDD145 pKa = 3.17 ILSRR149 pKa = 11.84 SRR151 pKa = 11.84 EE152 pKa = 3.99 FIFKK156 pKa = 10.1 VIRR159 pKa = 11.84 SPHH162 pKa = 6.26 DD163 pKa = 3.26 PEE165 pKa = 6.47 IYY167 pKa = 8.87 LTPEE171 pKa = 4.66 AIDD174 pKa = 4.22 AYY176 pKa = 9.96 VASFVEE182 pKa = 4.2 QEE184 pKa = 4.41 TVEE187 pKa = 4.07 EE188 pKa = 4.61 VPWSEE193 pKa = 4.43 EE194 pKa = 3.49 ATADD198 pKa = 3.28 IAEE201 pKa = 4.37 TLTLEE206 pKa = 4.8 RR207 pKa = 11.84 DD208 pKa = 3.57 AQRR211 pKa = 11.84 LAKK214 pKa = 10.04 FLEE217 pKa = 4.68 PEE219 pKa = 4.17 AVDD222 pKa = 4.66 LPEE225 pKa = 6.35 GPDD228 pKa = 4.9 ADD230 pKa = 4.86 DD231 pKa = 3.36 EE232 pKa = 4.55 WVPGVPEE239 pKa = 4.68 GEE241 pKa = 4.34 PEE243 pKa = 4.14 TTVEE247 pKa = 4.91 SEE249 pKa = 4.07 TADD252 pKa = 3.47 APDD255 pKa = 3.17 IAEE258 pKa = 4.67 PEE260 pKa = 4.42 TVDD263 pKa = 4.52 AEE265 pKa = 4.28 ATEE268 pKa = 4.25 SEE270 pKa = 4.65 IYY272 pKa = 10.52 YY273 pKa = 10.55 SPEE276 pKa = 3.25 TDD278 pKa = 2.95 MPTFLRR284 pKa = 11.84 DD285 pKa = 3.41 LTPVNGAEE293 pKa = 3.81 EE294 pKa = 4.45 DD295 pKa = 3.59 GRR297 pKa = 11.84 LFATVDD303 pKa = 3.39 ALNEE307 pKa = 3.97 RR308 pKa = 11.84 LAGIPAGGSLVLWGLDD324 pKa = 2.87 NGLNHH329 pKa = 6.57 SADD332 pKa = 4.5 GYY334 pKa = 10.77 SSTQIRR340 pKa = 11.84 IALRR344 pKa = 11.84 RR345 pKa = 11.84 GKK347 pKa = 9.32 PAATYY352 pKa = 8.87 WYY354 pKa = 9.2 KK355 pKa = 10.71 DD356 pKa = 3.12 APRR359 pKa = 11.84 RR360 pKa = 11.84 QEE362 pKa = 3.58 DD363 pKa = 3.3 SRR365 pKa = 11.84 ALFIGSAVHH374 pKa = 6.81 AALLEE379 pKa = 4.1 PEE381 pKa = 4.47 RR382 pKa = 11.84 FATEE386 pKa = 4.02 YY387 pKa = 11.31 ACAPSVNLRR396 pKa = 11.84 TSEE399 pKa = 4.01 GKK401 pKa = 8.05 EE402 pKa = 3.66 TLASFEE408 pKa = 4.51 CDD410 pKa = 2.88 CATRR414 pKa = 11.84 NLIPIKK420 pKa = 10.58 RR421 pKa = 11.84 EE422 pKa = 3.97 DD423 pKa = 3.9 FDD425 pKa = 4.7 TVCMMRR431 pKa = 11.84 DD432 pKa = 3.26 SVLAFPVVRR441 pKa = 11.84 LLLSSGVGEE450 pKa = 4.72 LSVFWRR456 pKa = 11.84 MEE458 pKa = 3.64 NGLLLKK464 pKa = 10.12 IRR466 pKa = 11.84 PDD468 pKa = 3.1 WLGVYY473 pKa = 9.91 QDD475 pKa = 4.44 APFMLDD481 pKa = 3.52 VKK483 pKa = 10.28 TLDD486 pKa = 3.75 DD487 pKa = 3.88 VNDD490 pKa = 5.01 FGRR493 pKa = 11.84 QAHH496 pKa = 4.86 EE497 pKa = 4.08 HH498 pKa = 6.64 GYY500 pKa = 10.09 HH501 pKa = 5.8 IQAAYY506 pKa = 9.87 YY507 pKa = 10.23 GFVLEE512 pKa = 5.15 HH513 pKa = 5.99 VFGMVINGLFCTIGKK528 pKa = 8.5 RR529 pKa = 11.84 TEE531 pKa = 3.86 CGRR534 pKa = 11.84 YY535 pKa = 8.5 PVEE538 pKa = 5.12 LGPMDD543 pKa = 4.43 NGDD546 pKa = 3.92 SEE548 pKa = 5.18 EE549 pKa = 4.54 GLLQVHH555 pKa = 6.86 DD556 pKa = 5.72 AITVLNGPDD565 pKa = 4.62 DD566 pKa = 6.03 DD567 pKa = 4.11 IASCFTVFSRR577 pKa = 11.84 PSWAKK582 pKa = 8.98 QADD585 pKa = 3.9 RR586 pKa = 11.84 KK587 pKa = 10.3 RR588 pKa = 11.84 RR589 pKa = 11.84 EE590 pKa = 4.1 AQEE593 pKa = 3.91 VMAA596 pKa = 6.07
Molecular weight: 66.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.398
IPC2_protein 4.266
IPC_protein 4.24
Toseland 4.062
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.062
Rodwell 4.075
Grimsley 3.973
Solomon 4.19
Lehninger 4.139
Nozaki 4.291
DTASelect 4.457
Thurlkill 4.075
EMBOSS 4.075
Sillero 4.355
Patrickios 3.783
IPC_peptide 4.19
IPC2_peptide 4.342
IPC2.peptide.svr19 4.27
Protein with the highest isoelectric point:
>tr|A3E2L4|A3E2L4_9CAUD Gp55 OS=Sodalis phage phiSG1 OX=373126 PE=3 SV=1
MM1 pKa = 7.53 AMKK4 pKa = 10.3 DD5 pKa = 2.88 KK6 pKa = 10.99 SYY8 pKa = 10.37 AQRR11 pKa = 11.84 RR12 pKa = 11.84 FEE14 pKa = 3.76 RR15 pKa = 11.84 TRR17 pKa = 11.84 AHH19 pKa = 6.63 FEE21 pKa = 4.09 KK22 pKa = 10.73 INDD25 pKa = 3.52 DD26 pKa = 3.34 RR27 pKa = 11.84 ALARR31 pKa = 11.84 KK32 pKa = 9.08 VSKK35 pKa = 10.26 AWAKK39 pKa = 8.53 ITQPEE44 pKa = 4.16 QTLKK48 pKa = 10.78 AYY50 pKa = 9.43 RR51 pKa = 11.84 QQIIDD56 pKa = 3.18 ATEE59 pKa = 3.96 RR60 pKa = 11.84 YY61 pKa = 8.6 NALKK65 pKa = 10.54 KK66 pKa = 9.12 SARR69 pKa = 11.84 YY70 pKa = 9.69 RR71 pKa = 11.84 MAIARR76 pKa = 4.05
Molecular weight: 9.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.897
IPC_protein 10.657
Toseland 10.862
ProMoST 10.745
Dawson 10.95
Bjellqvist 10.628
Wikipedia 11.14
Rodwell 11.257
Grimsley 10.994
Solomon 11.052
Lehninger 11.023
Nozaki 10.818
DTASelect 10.628
Thurlkill 10.847
EMBOSS 11.257
Sillero 10.877
Patrickios 10.994
IPC_peptide 11.067
IPC2_peptide 9.37
IPC2.peptide.svr19 8.544
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
12786
71
724
250.7
27.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.48 ± 0.688
1.22 ± 0.151
5.85 ± 0.328
6.796 ± 0.364
3.582 ± 0.22
7.164 ± 0.34
2.112 ± 0.21
5.279 ± 0.259
5.529 ± 0.331
8.376 ± 0.285
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.464 ± 0.148
4.012 ± 0.283
4.106 ± 0.345
4.974 ± 0.409
6.499 ± 0.405
5.608 ± 0.254
5.545 ± 0.212
6.233 ± 0.271
1.251 ± 0.122
2.917 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here