Aerococcus urinaehominis
Average proteome isoelectric point is 5.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1595 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0X8FLI7|A0A0X8FLI7_9LACT ATP-dependent helicase/nuclease subunit A OS=Aerococcus urinaehominis OX=128944 GN=addA PE=3 SV=1
MM1 pKa = 7.12 SFSVKK6 pKa = 10.67 SMLGTSLATTAALVAIAPSVVADD29 pKa = 4.87 DD30 pKa = 4.58 YY31 pKa = 10.86 TIQWGDD37 pKa = 3.42 TLTDD41 pKa = 4.04 LAQKK45 pKa = 10.83 HH46 pKa = 5.41 NVTLDD51 pKa = 3.39 DD52 pKa = 4.32 LKK54 pKa = 11.08 AANGITDD61 pKa = 3.59 SGDD64 pKa = 3.79 LIIAGHH70 pKa = 5.5 TLVIPEE76 pKa = 4.74 LPQVVGQSQATDD88 pKa = 3.5 GQNVYY93 pKa = 8.46 TVQAGDD99 pKa = 3.83 TLNKK103 pKa = 9.76 IAAQFGTTADD113 pKa = 3.49 SLRR116 pKa = 11.84 ALNNIQGDD124 pKa = 4.47 LIFIGQQLVVAGQVAEE140 pKa = 4.25 AATVAPVVEE149 pKa = 4.8 EE150 pKa = 3.9 PAVAEE155 pKa = 4.18 EE156 pKa = 4.54 TTVEE160 pKa = 3.95 AEE162 pKa = 4.1 PAVVEE167 pKa = 4.06 TDD169 pKa = 3.35 KK170 pKa = 11.57 LDD172 pKa = 3.86 EE173 pKa = 5.05 AEE175 pKa = 4.25 LAQAVAYY182 pKa = 9.62 QAEE185 pKa = 4.61 EE186 pKa = 3.88 VAPIAEE192 pKa = 4.34 EE193 pKa = 4.04 LVAPASEE200 pKa = 3.98 AVGEE204 pKa = 4.22 QEE206 pKa = 4.82 VAATSSNEE214 pKa = 3.99 VEE216 pKa = 4.31 STEE219 pKa = 3.92 ATVTEE224 pKa = 4.53 EE225 pKa = 4.1 VAPKK229 pKa = 10.72 AEE231 pKa = 4.52 AKK233 pKa = 10.56 EE234 pKa = 4.06 EE235 pKa = 4.45 TPVATAEE242 pKa = 4.1 TAAAAPASAEE252 pKa = 3.91 ATAVDD257 pKa = 4.32 AEE259 pKa = 4.42 VAPAVQAAPAPAQEE273 pKa = 4.33 TVTVTEE279 pKa = 4.83 AEE281 pKa = 4.07 TAPIEE286 pKa = 4.07 EE287 pKa = 4.85 AAPVAQPVAYY297 pKa = 7.78 EE298 pKa = 4.15 APAVEE303 pKa = 4.59 APAPVKK309 pKa = 10.67 EE310 pKa = 4.08 EE311 pKa = 4.29 APAEE315 pKa = 4.04 TAPVEE320 pKa = 4.29 EE321 pKa = 4.57 EE322 pKa = 4.42 VVAEE326 pKa = 4.29 PVQEE330 pKa = 4.07 AAPAAEE336 pKa = 4.28 TQHH339 pKa = 6.33 NGSSVLEE346 pKa = 4.26 VADD349 pKa = 3.73 QYY351 pKa = 12.04 VGTPYY356 pKa = 10.63 VWGGRR361 pKa = 11.84 QPGGFDD367 pKa = 3.08 CSGFVQYY374 pKa = 10.51 VYY376 pKa = 10.85 RR377 pKa = 11.84 EE378 pKa = 4.07 AEE380 pKa = 4.01 GRR382 pKa = 11.84 EE383 pKa = 4.06 VGSWTGEE390 pKa = 3.7 QQYY393 pKa = 10.8 AGPQIAVEE401 pKa = 4.33 SAQQGDD407 pKa = 3.9 LLFWGDD413 pKa = 3.63 YY414 pKa = 9.95 GNPYY418 pKa = 9.59 HH419 pKa = 6.62 VAISTGDD426 pKa = 3.01 NGYY429 pKa = 9.11 IHH431 pKa = 7.01 SPQPGQTVGYY441 pKa = 8.05 GTVSEE446 pKa = 4.24 YY447 pKa = 9.95 WYY449 pKa = 9.93 PSFAVQMM456 pKa = 4.47
Molecular weight: 47.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.757
IPC_protein 3.719
Toseland 3.541
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.528
Rodwell 3.554
Grimsley 3.452
Solomon 3.656
Lehninger 3.617
Nozaki 3.783
DTASelect 3.897
Thurlkill 3.554
EMBOSS 3.541
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.668
IPC2_peptide 3.821
IPC2.peptide.svr19 3.745
Protein with the highest isoelectric point:
>tr|A0A0X8FMZ0|A0A0X8FMZ0_9LACT O-antigen polymerase OS=Aerococcus urinaehominis OX=128944 GN=AWM75_05845 PE=4 SV=1
MM1 pKa = 7.46 LRR3 pKa = 11.84 ALDD6 pKa = 3.37 KK7 pKa = 11.11 HH8 pKa = 6.08 GLVKK12 pKa = 10.64 GLMMGTGRR20 pKa = 11.84 ILRR23 pKa = 11.84 CHH25 pKa = 6.51 PFARR29 pKa = 11.84 GGFDD33 pKa = 3.28 PVPDD37 pKa = 3.63 HH38 pKa = 6.08 FTLRR42 pKa = 11.84 RR43 pKa = 11.84 NRR45 pKa = 11.84 SNDD48 pKa = 3.01 LPEE51 pKa = 5.48 AEE53 pKa = 4.39 KK54 pKa = 10.5 QALIADD60 pKa = 3.78 HH61 pKa = 6.58 HH62 pKa = 6.56 RR63 pKa = 11.84 RR64 pKa = 11.84 HH65 pKa = 5.77 HH66 pKa = 5.89 HH67 pKa = 5.5
Molecular weight: 7.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.32
IPC2_protein 9.428
IPC_protein 10.277
Toseland 10.95
ProMoST 10.906
Dawson 10.965
Bjellqvist 10.745
Wikipedia 11.242
Rodwell 11.023
Grimsley 10.994
Solomon 11.242
Lehninger 11.199
Nozaki 10.935
DTASelect 10.745
Thurlkill 10.921
EMBOSS 11.374
Sillero 10.921
Patrickios 10.847
IPC_peptide 11.257
IPC2_peptide 9.999
IPC2.peptide.svr19 9.029
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1595
0
1595
516534
37
3575
323.8
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.881 ± 0.09
0.594 ± 0.017
6.687 ± 0.058
5.898 ± 0.063
3.937 ± 0.047
6.859 ± 0.066
2.002 ± 0.027
6.959 ± 0.059
5.234 ± 0.067
9.986 ± 0.096
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.495 ± 0.04
4.373 ± 0.041
3.694 ± 0.052
5.747 ± 0.075
4.204 ± 0.044
5.725 ± 0.042
5.131 ± 0.083
6.879 ± 0.05
0.868 ± 0.023
3.849 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here