Lishi Spider Virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Lispiviridae; Arlivirus; Lishi arlivirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KRF3|A0A0B5KRF3_9MONO RNA-directed RNA polymerase L OS=Lishi Spider Virus 2 OX=1608058 GN=L PE=3 SV=1
MM1 pKa = 7.75CSNFKK6 pKa = 10.83KK7 pKa = 10.74FGICSNGMRR16 pKa = 11.84CKK18 pKa = 10.4KK19 pKa = 10.09FHH21 pKa = 7.56DD22 pKa = 4.19YY23 pKa = 11.56EE24 pKa = 4.43EE25 pKa = 5.38DD26 pKa = 3.53NLDD29 pKa = 3.43IQVGLNLIMGAISGISEE46 pKa = 4.17KK47 pKa = 10.56IKK49 pKa = 10.87NIEE52 pKa = 3.88NKK54 pKa = 9.47INEE57 pKa = 4.34IEE59 pKa = 4.27VVMSQKK65 pKa = 10.6PKK67 pKa = 10.37FQEE70 pKa = 4.13FPNNSDD76 pKa = 3.49SLNSRR81 pKa = 11.84LNMISEE87 pKa = 4.14QLDD90 pKa = 4.0TIKK93 pKa = 11.0GSIHH97 pKa = 5.13TQGIPLSTTFSNFRR111 pKa = 11.84GKK113 pKa = 9.56WSTTQNTNPKK123 pKa = 9.47GNKK126 pKa = 8.14TKK128 pKa = 10.8SSLNEE133 pKa = 3.71RR134 pKa = 11.84SIDD137 pKa = 3.67LYY139 pKa = 11.32EE140 pKa = 4.88KK141 pKa = 10.5IPAKK145 pKa = 10.03GAKK148 pKa = 10.06SMMNKK153 pKa = 9.52KK154 pKa = 10.32VSGKK158 pKa = 8.09EE159 pKa = 3.74AEE161 pKa = 4.15NRR163 pKa = 11.84TIRR166 pKa = 11.84NDD168 pKa = 3.1SHH170 pKa = 7.27EE171 pKa = 4.52SDD173 pKa = 4.51SDD175 pKa = 3.77NTSKK179 pKa = 11.16NSGGSFIKK187 pKa = 10.48EE188 pKa = 3.84LVSVQIEE195 pKa = 4.55SQSGTSEE202 pKa = 4.05SPEE205 pKa = 3.67LSGEE209 pKa = 4.16RR210 pKa = 11.84FQKK213 pKa = 10.68PFMNMEE219 pKa = 4.88LAPPLSPTGSCEE231 pKa = 4.15HH232 pKa = 6.83MEE234 pKa = 4.3TEE236 pKa = 5.18NPPEE240 pKa = 4.19HH241 pKa = 7.25QGMTDD246 pKa = 3.05NDD248 pKa = 3.7YY249 pKa = 11.63SDD251 pKa = 5.02DD252 pKa = 3.53NRR254 pKa = 11.84KK255 pKa = 9.23IFTQKK260 pKa = 9.23TPAIEE265 pKa = 4.14SFKK268 pKa = 10.99KK269 pKa = 10.37DD270 pKa = 3.5DD271 pKa = 4.29EE272 pKa = 4.93LDD274 pKa = 3.09IHH276 pKa = 6.92

Molecular weight: 30.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KK20|A0A0B5KK20_9MONO ORF2 OS=Lishi Spider Virus 2 OX=1608058 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.2FRR5 pKa = 11.84NILIIFISMLSPGLFIEE22 pKa = 5.32RR23 pKa = 11.84YY24 pKa = 9.33HH25 pKa = 6.87PIFFEE30 pKa = 4.3KK31 pKa = 10.64YY32 pKa = 9.21GVLIEE37 pKa = 4.36PNGVVTSYY45 pKa = 11.8DD46 pKa = 2.99KK47 pKa = 11.35VKK49 pKa = 10.24YY50 pKa = 8.89VTLIYY55 pKa = 10.24HH56 pKa = 6.47FPGLQKK62 pKa = 10.76AVTPSHH68 pKa = 6.75CNRR71 pKa = 11.84NASEE75 pKa = 3.74QLYY78 pKa = 10.68RR79 pKa = 11.84NTLKK83 pKa = 10.75HH84 pKa = 6.01QIKK87 pKa = 10.16IMYY90 pKa = 9.49DD91 pKa = 3.79LIPSSTHH98 pKa = 6.0TIMSNYY104 pKa = 8.9CSKK107 pKa = 10.62HH108 pKa = 5.55HH109 pKa = 6.55ALCLDD114 pKa = 4.03LEE116 pKa = 4.69KK117 pKa = 10.52KK118 pKa = 10.11DD119 pKa = 3.59RR120 pKa = 11.84PKK122 pKa = 10.75RR123 pKa = 11.84QIIAAIAALSGIAGLAISAYY143 pKa = 10.02DD144 pKa = 3.65WISSSEE150 pKa = 4.14LKK152 pKa = 10.67NHH154 pKa = 6.39LDD156 pKa = 3.35KK157 pKa = 11.47VSEE160 pKa = 4.9HH161 pKa = 5.47ITKK164 pKa = 9.74IDD166 pKa = 3.32NKK168 pKa = 10.93LIIQEE173 pKa = 4.02NLDD176 pKa = 3.63YY177 pKa = 11.34KK178 pKa = 9.96LTKK181 pKa = 10.34LSNDD185 pKa = 3.63IIKK188 pKa = 8.48KK189 pKa = 8.37TSMSFEE195 pKa = 4.3NYY197 pKa = 8.99HH198 pKa = 6.35KK199 pKa = 10.32FVKK202 pKa = 10.37EE203 pKa = 3.83YY204 pKa = 10.61VCQSSQFNNFLSHH217 pKa = 6.83HH218 pKa = 6.07VATTHH223 pKa = 7.71LEE225 pKa = 4.06MEE227 pKa = 4.39LQALQRR233 pKa = 11.84LINGEE238 pKa = 3.93PDD240 pKa = 3.21SYY242 pKa = 11.01IINAEE247 pKa = 4.05TLEE250 pKa = 4.9SILSSDD256 pKa = 3.86TEE258 pKa = 4.4VEE260 pKa = 3.88RR261 pKa = 11.84SIYY264 pKa = 10.85SSDD267 pKa = 3.21PSLFYY272 pKa = 10.6QVVRR276 pKa = 11.84SNLIHH281 pKa = 7.11SDD283 pKa = 3.1IEE285 pKa = 3.95KK286 pKa = 10.85SLFAFILEE294 pKa = 4.12VPIIQEE300 pKa = 4.14TMISPLYY307 pKa = 10.08KK308 pKa = 10.05VYY310 pKa = 11.12NGGWEE315 pKa = 4.3SKK317 pKa = 10.89GILHH321 pKa = 7.37KK322 pKa = 10.23IDD324 pKa = 4.83LPDD327 pKa = 3.2SFYY330 pKa = 10.97LYY332 pKa = 10.96SSLDD336 pKa = 3.52DD337 pKa = 3.56VDD339 pKa = 3.71YY340 pKa = 11.13HH341 pKa = 7.08AISLGKK347 pKa = 9.53SNCWKK352 pKa = 9.59RR353 pKa = 11.84NEE355 pKa = 4.49VVVCDD360 pKa = 4.0NSKK363 pKa = 10.92HH364 pKa = 5.36MMTEE368 pKa = 4.82DD369 pKa = 4.4MICLNSILKK378 pKa = 10.12RR379 pKa = 11.84EE380 pKa = 4.32YY381 pKa = 10.94YY382 pKa = 10.31DD383 pKa = 3.27SCDD386 pKa = 2.99IKK388 pKa = 10.75FSKK391 pKa = 9.08FTRR394 pKa = 11.84RR395 pKa = 11.84KK396 pKa = 8.94FVVKK400 pKa = 10.19ALSGVLVCGDD410 pKa = 4.51LEE412 pKa = 4.57VKK414 pKa = 10.32IITSAGSQFHH424 pKa = 6.23YY425 pKa = 10.45IKK427 pKa = 10.89NNKK430 pKa = 8.03QEE432 pKa = 4.07NNYY435 pKa = 8.38TKK437 pKa = 10.24YY438 pKa = 10.79YY439 pKa = 9.9SYY441 pKa = 11.86NEE443 pKa = 3.89FKK445 pKa = 10.87QIIVGNTIVSTNINKK460 pKa = 8.56MPIVRR465 pKa = 11.84RR466 pKa = 11.84NDD468 pKa = 3.81TISVNFDD475 pKa = 3.49YY476 pKa = 10.96DD477 pKa = 4.29LKK479 pKa = 10.16WADD482 pKa = 4.0KK483 pKa = 10.29ILHH486 pKa = 5.44EE487 pKa = 5.16RR488 pKa = 11.84PWTSLKK494 pKa = 10.43EE495 pKa = 3.91INLLKK500 pKa = 10.86DD501 pKa = 3.2SGFEE505 pKa = 4.04QYY507 pKa = 10.7HH508 pKa = 5.05GTISHH513 pKa = 6.97NLNQWSVFLWVTIILCLIILIYY535 pKa = 10.71LLIASRR541 pKa = 11.84RR542 pKa = 11.84KK543 pKa = 8.39ITSLEE548 pKa = 4.0DD549 pKa = 3.22RR550 pKa = 11.84LKK552 pKa = 10.13LTNGIIKK559 pKa = 10.49LSNKK563 pKa = 9.79RR564 pKa = 11.84SILL567 pKa = 3.69

Molecular weight: 65.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3044

276

2201

1014.7

117.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.365 ± 0.319

1.708 ± 0.062

5.519 ± 0.128

6.603 ± 0.599

4.698 ± 0.322

4.008 ± 0.514

2.497 ± 0.385

9.625 ± 0.568

8.279 ± 0.386

10.414 ± 1.366

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.43

7.096 ± 0.468

3.187 ± 0.373

3.318 ± 0.189

4.172 ± 0.456

9.428 ± 0.878

5.158 ± 0.201

4.402 ± 0.534

1.084 ± 0.172

4.238 ± 0.747
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski