Paenibacillus montanisoli
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5567 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A328U5M5|A0A328U5M5_9BACL Uncharacterized protein OS=Paenibacillus montanisoli OX=2081970 GN=DL346_01050 PE=4 SV=1
MM1 pKa = 7.23 QNDD4 pKa = 3.54 TALFFQFGDD13 pKa = 3.66 NNSASLACSTLRR25 pKa = 11.84 EE26 pKa = 4.11 LGYY29 pKa = 11.04 DD30 pKa = 3.45 PVIQQDD36 pKa = 3.67 AGVHH40 pKa = 3.64 IHH42 pKa = 6.03 MRR44 pKa = 11.84 GSDD47 pKa = 3.47 LTSALEE53 pKa = 3.69 IAQAHH58 pKa = 6.54 GGQLAVQSSIPDD70 pKa = 3.22 IEE72 pKa = 4.49 LTNNAYY78 pKa = 10.91 NMDD81 pKa = 4.92 SITIPAHH88 pKa = 5.23 VVNEE92 pKa = 3.92 DD93 pKa = 4.59 LIAQEE98 pKa = 4.52 EE99 pKa = 4.44 AQSGLRR105 pKa = 11.84 NWDD108 pKa = 3.18 DD109 pKa = 4.51 DD110 pKa = 4.31 APQEE114 pKa = 4.82 DD115 pKa = 5.03 SFIPDD120 pKa = 3.92 DD121 pKa = 3.72 GSYY124 pKa = 10.99 GYY126 pKa = 10.73 FSGDD130 pKa = 2.86 VHH132 pKa = 5.92 TT133 pKa = 5.42
Molecular weight: 14.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.91
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A328TXL6|A0A328TXL6_9BACL Uncharacterized protein OS=Paenibacillus montanisoli OX=2081970 GN=DL346_17695 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.82 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.48 GFRR19 pKa = 11.84 KK20 pKa = 10.06 RR21 pKa = 11.84 MASKK25 pKa = 10.64 NGRR28 pKa = 11.84 KK29 pKa = 9.12 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.84 VLSAA44 pKa = 4.11
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5567
0
5567
1805433
25
2578
324.3
36.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.934 ± 0.042
0.845 ± 0.011
5.182 ± 0.023
6.604 ± 0.032
4.178 ± 0.022
7.645 ± 0.03
2.179 ± 0.015
6.51 ± 0.032
5.088 ± 0.028
9.961 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.838 ± 0.015
3.651 ± 0.025
4.223 ± 0.02
3.669 ± 0.02
5.339 ± 0.028
6.123 ± 0.026
5.224 ± 0.024
6.968 ± 0.028
1.385 ± 0.014
3.452 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here